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The map label for this gene is gshA [H]

Identifier: 161526312

GI number: 161526312

Start: 3435272

End: 3436885

Strand: Reverse

Name: gshA [H]

Synonym: Bmul_3143

Alternate gene names: 161526312

Gene position: 3436885-3435272 (Counterclockwise)

Preceding gene: 161526313

Following gene: 161526310

Centisome position: 99.66

GC content: 66.17

Gene sequence:

>1614_bases
ATGTCGAACACCATGACTCACCGCCAGTCCGAACTGTTGCTGAATCGCCTCGATGCGCTGAGCACGGGCCCGACGCGGCA
GCATCTGCCCGACGGCCTGCGCGGCATCGAAAAGGAAAGCCTGCGCGTGACGCGCGACGGGATGATCGCGTTCACGCCGC
ACCCGCGCGCGCTTGGTTCGGCTCTCACGCATCCGTCGTTGACGACCGATTATTCCGAAGCGCTGCTCGAGCTGATCACA
CCCGCGGAAGGAGACGCCGCGCTGACGCTCGAACGCCTCGACGAACTGCATCGCTACGTGTACGCGTCGCTCGGCGACGA
GCTGTTGTGGAACAACTCGATGCCCGGCCTGCTCCCGGCCGACGACGAGATCCCGATCGCGAACTACGGCACGTCGAACA
TCGGTCGGCTGAAGACGGTATATCGCCGCGGCCTCGCGTATCGCTACGGCCGCACGATGCAATGCATCGCGGGCATCCAC
TACAACTACTCGCTGCACGAGGAAGTGTGGCGGCGCCTGCATGCGGACGAAGGATCGACCGCGACGCTCGTCGACTATCA
GTCCGAGCGCTATCTCGCGCTGATCCGCAACTTCCGGCGCCGCAGCTGGCTGCTGATGTATCTGTTCGGCGCATCGCCCG
CGCTCGACACGAGGTTCCTGCGCGGCAAGCCGAACACGCTCGAAGCGTTCGACGCCGATACGCTGTACCGCCCGTATGCC
ACGAGCCTGCGCATGAGCGATCTCGGCTACTCGAACACGACCGCACAGGCCGCGCTGCACGTCGACTACAACACGCTGCC
CGGCTATCTCGACGCGCTGTCGAAGGCCGTCAGCGAGCCGTATCCGCCGTACGAGGCGATCGGCACGCATCGCGACGGCG
AGTGGATCCAGATCAACACGAACGTGCTGCAGATCGAGAACGAGTTCTACTCGACGATCCGGCCGAAGCGCGTCACCTAT
TCGGGTGAGCGTCCGCTGCATGCGCTCGCCTCGCGCGGCGTGCAGTACATCGAAGTGCGCTGCCTCGACATCGATCCGTT
CGAGCCGACCGGCATCGCAATCGACACCGCGCGCTTTCTCGACGCGTTCCTGCTCGCATGCGCGCTCGACGCGAGCCCGA
CGCTTGACTGCGACGCGTACAAGGAAGCGAATGCGAACTTCGCGAGCGTGACGATGGAGGGGCGCAAGCCGGGGCTGATG
CTGACGCGCGACGGCGAGCCGATCGCGATGCGCGCGTGGGCCGATGCGCTGATGGCCGACATCGAAACGATCGGCCGCCG
TCTCGACGAGATTCGCGGCGACGATGCGCATGCGCGCGCGATCGCCGCGCAGCGCGAGAAACTCGCCGATCCGGAACGCA
CGCCGTCGGCGCGCGTGCTGCGCACAATGCGCGAGAACGGCCAGTCGTTCCTCGCGTTCGCGCGCGCGCAGAGCGAAGCG
CATGCCGCGTACTACCGTGCGCATCGGCCGTCGGCGGACGCGATGCGCGCATCGACCGCACTCGCCGAGCAGTCGCTCGC
CGAGCAGGCGGAGCTGGAAGCGAAGGAGGCCGGATCGTTCGATGCGTTCGTCGCGGCCTATCGTGCGTATACGCTGAACC
GCTTCAGCGTCTGA

Upstream 100 bases:

>100_bases
GCTTTCGGCGAGATGTTAAAGTGCCCGCTACCAACGAAGCGACCAATAATCCAGCCGGCCGCGCGGCATCGTCCTGCGGC
CGTCCCGCACTTGCGCATCC

Downstream 100 bases:

>100_bases
CGTGCGTCGCGGCGTAGTGCGCCGCGCACGTCGGCGCGCGCGGGGCGATCTCGCGGCGGGCGTCGCAGCACGTGCGGCGC
GTTCGGCGGCGCGCCATCAA

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSNTMTHRQSELLLNRLDALSTGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTDYSEALLELIT
PAEGDAALTLERLDELHRYVYASLGDELLWNNSMPGLLPADDEIPIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIH
YNYSLHEEVWRRLHADEGSTATLVDYQSERYLALIRNFRRRSWLLMYLFGASPALDTRFLRGKPNTLEAFDADTLYRPYA
TSLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTY
SGERPLHALASRGVQYIEVRCLDIDPFEPTGIAIDTARFLDAFLLACALDASPTLDCDAYKEANANFASVTMEGRKPGLM
LTRDGEPIAMRAWADALMADIETIGRRLDEIRGDDAHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFARAQSEA
HAAYYRAHRPSADAMRASTALAEQSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV

Sequences:

>Translated_537_residues
MSNTMTHRQSELLLNRLDALSTGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTDYSEALLELIT
PAEGDAALTLERLDELHRYVYASLGDELLWNNSMPGLLPADDEIPIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIH
YNYSLHEEVWRRLHADEGSTATLVDYQSERYLALIRNFRRRSWLLMYLFGASPALDTRFLRGKPNTLEAFDADTLYRPYA
TSLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTY
SGERPLHALASRGVQYIEVRCLDIDPFEPTGIAIDTARFLDAFLLACALDASPTLDCDAYKEANANFASVTMEGRKPGLM
LTRDGEPIAMRAWADALMADIETIGRRLDEIRGDDAHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFARAQSEA
HAAYYRAHRPSADAMRASTALAEQSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV
>Mature_536_residues
SNTMTHRQSELLLNRLDALSTGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTDYSEALLELITP
AEGDAALTLERLDELHRYVYASLGDELLWNNSMPGLLPADDEIPIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHY
NYSLHEEVWRRLHADEGSTATLVDYQSERYLALIRNFRRRSWLLMYLFGASPALDTRFLRGKPNTLEAFDADTLYRPYAT
SLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEFYSTIRPKRVTYS
GERPLHALASRGVQYIEVRCLDIDPFEPTGIAIDTARFLDAFLLACALDASPTLDCDAYKEANANFASVTMEGRKPGLML
TRDGEPIAMRAWADALMADIETIGRRLDEIRGDDAHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFARAQSEAH
AAYYRAHRPSADAMRASTALAEQSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=504, Percent_Identity=42.6587301587302, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 60090; Mature: 59959

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTMTHRQSELLLNRLDALSTGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGS
CCCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEEEECCCCHHHHH
ALTHPSLTTDYSEALLELITPAEGDAALTLERLDELHRYVYASLGDELLWNNSMPGLLPA
HHCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
DDEIPIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLHEEVWRRLHADEGST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCE
ATLVDYQSERYLALIRNFRRRSWLLMYLFGASPALDTRFLRGKPNTLEAFDADTLYRPYA
EEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCHH
TSLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINT
HHEEHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEC
NVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIAIDTARFL
CEEEECHHHHHHCCCCEEEECCCCCHHHHHHCCCEEEEEEEEECCCCCCCCEEEHHHHHH
DAFLLACALDASPTLDCDAYKEANANFASVTMEGRKPGLMLTRDGEPIAMRAWADALMAD
HHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEECCCCCCEEEEECCCCEEHHHHHHHHHHH
IETIGRRLDEIRGDDAHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFARAQSEA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
HAAYYRAHRPSADAMRASTALAEQSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNTMTHRQSELLLNRLDALSTGPTRQHLPDGLRGIEKESLRVTRDGMIAFTPHPRALGS
CCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEEEECCCCHHHHH
ALTHPSLTTDYSEALLELITPAEGDAALTLERLDELHRYVYASLGDELLWNNSMPGLLPA
HHCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
DDEIPIANYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLHEEVWRRLHADEGST
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCE
ATLVDYQSERYLALIRNFRRRSWLLMYLFGASPALDTRFLRGKPNTLEAFDADTLYRPYA
EEEEECCCCHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCHH
TSLRMSDLGYSNTTAQAALHVDYNTLPGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINT
HHEEHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEC
NVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIAIDTARFL
CEEEECHHHHHHCCCCEEEECCCCCHHHHHHCCCEEEEEEEEECCCCCCCCEEEHHHHHH
DAFLLACALDASPTLDCDAYKEANANFASVTMEGRKPGLMLTRDGEPIAMRAWADALMAD
HHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEECCCCCCEEEEECCCCEEHHHHHHHHHHH
IETIGRRLDEIRGDDAHARAIAAQREKLADPERTPSARVLRTMRENGQSFLAFARAQSEA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
HAAYYRAHRPSADAMRASTALAEQSLAEQAELEAKEAGSFDAFVAAYRAYTLNRFSV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA