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The map label for this gene is ada [H]

Identifier: 161526314

GI number: 161526314

Start: 3438147

End: 3439244

Strand: Reverse

Name: ada [H]

Synonym: Bmul_3145

Alternate gene names: 161526314

Gene position: 3439244-3438147 (Counterclockwise)

Preceding gene: 161526316

Following gene: 161526313

Centisome position: 99.73

GC content: 71.31

Gene sequence:

>1098_bases
ATGAAAACCGCCTATCCGACCGACGATGCCCGCTGGGGCGCCGTGACCGCCCGCGATCCGCACGCGGACGGCGCGTTTTT
CTACGCAGTCCGCACGACGGGCGTGTTCTGCCGTCCGAGCTGCGCGTCGCGGCTGCCGCGCCGCGAGCACGTGGCGTTCT
TCGACGATCCGGCCGCCGCGCGGGCGGCAGGCTTCCGGCCCTGCAAACGCTGTCAGCCGGAAGGGCTGCCGCGCGAGCTC
GAGATCGTCAATCGCGCCTGCGCGGTGCTCGATGCCCATTCGGAGCGTTTGACGCTGCAGCAGCTCAGTGACGCCGTGCA
TGTGAGCCCGTTTCATCTTCAGCGGCTGTTCAAGCGCGTGGTCGGCGTGTCGCCGCGGCAATACCAGGCCGCACAGCGCG
GCGCCGCGCTGCGCCAGGCGCTGCAGCGCGGGCAGCCCGTCACGCGCGCCGCGGTCGACGCCGGTTTCAATTCGCCGTCG
CGGCTCTACGCGTCGGTGTCGCGCGAGCTCGGGATGGCGCCGTCCGCGTTTCGCCGGCAAGGCGCGGGGCTGCGGATCGA
CTATGCGACCGCGCCGACGTCGCTCGGCACGGTGCTCGTCGCCGCGACCGACAAAGGCATTTGCCGGATCGCGTTCGGCG
ACGAACCGGCCGCGCTCGTGCACGAACTGAAGGCGAGCTTCGCGCGTGCCGAGCTCGTCGAATCGCCGGCGCGGCTCGCA
CCGTTCATCGCGCAGATCGGCGCCTATCTGGACGGCACGCGGCACACCTTCGACCTGCCGCTCGACATCGCGCCGACGGC
GTTCCAGCAGCGCGTCTGGGAAGCGCTCACGCATATTCCGTACGGCGAGACGCGCAGCTATTCGGAGATTGCCGCGGCGC
TCGGCGCGCCGGGCGCGGTGCGCGCGGTTGCGTCCGCGTGCGCGTCGAATCCGGTCGCACTCGCGATTCCGTGTCATCGT
GTCGTGCAAAAGGGCGGCGCGCTCGCCGGCTATCGGTGGGGCGTGCGCCGCAAGGCGACGCTGCTCGCATCCGAAGCGCG
CCGCGCACGCGACGACGCACCGGCCACGGCCGCCATCGAGGAGGACAGCACAGCTTGA

Upstream 100 bases:

>100_bases
CCCGATTTGCGCTGCGGATGACAGCGCAAGATTCGGCGCGCGACCGGTCGTGCCGGCGCCTAAAGTAGGCACCGTCTACA
CTCGAGAGTGCGAGGTTACG

Downstream 100 bases:

>100_bases
GCATCGAAATCGCAACGATCGCATCGACAGCGGACGGCGGCCACGTGCCGCCGTCCGCGCAACTGGAACTGCGCTTCAAG
GCGCCGTACGACTGGCCGCG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKTAYPTDDARWGAVTARDPHADGAFFYAVRTTGVFCRPSCASRLPRREHVAFFDDPAAARAAGFRPCKRCQPEGLPREL
EIVNRACAVLDAHSERLTLQQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRQALQRGQPVTRAAVDAGFNSPS
RLYASVSRELGMAPSAFRRQGAGLRIDYATAPTSLGTVLVAATDKGICRIAFGDEPAALVHELKASFARAELVESPARLA
PFIAQIGAYLDGTRHTFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAAALGAPGAVRAVASACASNPVALAIPCHR
VVQKGGALAGYRWGVRRKATLLASEARRARDDAPATAAIEEDSTA

Sequences:

>Translated_365_residues
MKTAYPTDDARWGAVTARDPHADGAFFYAVRTTGVFCRPSCASRLPRREHVAFFDDPAAARAAGFRPCKRCQPEGLPREL
EIVNRACAVLDAHSERLTLQQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRQALQRGQPVTRAAVDAGFNSPS
RLYASVSRELGMAPSAFRRQGAGLRIDYATAPTSLGTVLVAATDKGICRIAFGDEPAALVHELKASFARAELVESPARLA
PFIAQIGAYLDGTRHTFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAAALGAPGAVRAVASACASNPVALAIPCHR
VVQKGGALAGYRWGVRRKATLLASEARRARDDAPATAAIEEDSTA
>Mature_365_residues
MKTAYPTDDARWGAVTARDPHADGAFFYAVRTTGVFCRPSCASRLPRREHVAFFDDPAAARAAGFRPCKRCQPEGLPREL
EIVNRACAVLDAHSERLTLQQLSDAVHVSPFHLQRLFKRVVGVSPRQYQAAQRGAALRQALQRGQPVTRAAVDAGFNSPS
RLYASVSRELGMAPSAFRRQGAGLRIDYATAPTSLGTVLVAATDKGICRIAFGDEPAALVHELKASFARAELVESPARLA
PFIAQIGAYLDGTRHTFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAAALGAPGAVRAVASACASNPVALAIPCHR
VVQKGGALAGYRWGVRRKATLLASEARRARDDAPATAAIEEDSTA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=149, Percent_Identity=36.241610738255, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788542, Length=343, Percent_Identity=48.6880466472303, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI1787596, Length=166, Percent_Identity=33.7349397590361, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=55.2941176470588, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=55.2941176470588, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=40.4761904761905, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17137554, Length=90, Percent_Identity=51.1111111111111, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39237; Mature: 39237

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAYPTDDARWGAVTARDPHADGAFFYAVRTTGVFCRPSCASRLPRREHVAFFDDPAAA
CCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCEEECCHHHHHCCCHHCCEEECCCHHH
RAAGFRPCKRCQPEGLPRELEIVNRACAVLDAHSERLTLQQLSDAVHVSPFHLQRLFKRV
HHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
VGVSPRQYQAAQRGAALRQALQRGQPVTRAAVDAGFNSPSRLYASVSRELGMAPSAFRRQ
HCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHC
GAGLRIDYATAPTSLGTVLVAATDKGICRIAFGDEPAALVHELKASFARAELVESPARLA
CCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHH
PFIAQIGAYLDGTRHTFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAAALGAPGAV
HHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHH
RAVASACASNPVALAIPCHRVVQKGGALAGYRWGVRRKATLLASEARRARDDAPATAAIE
HHHHHHHCCCCEEEEECHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCCCEEECC
EDSTA
CCCCC
>Mature Secondary Structure
MKTAYPTDDARWGAVTARDPHADGAFFYAVRTTGVFCRPSCASRLPRREHVAFFDDPAAA
CCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCEEECCHHHHHCCCHHCCEEECCCHHH
RAAGFRPCKRCQPEGLPRELEIVNRACAVLDAHSERLTLQQLSDAVHVSPFHLQRLFKRV
HHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
VGVSPRQYQAAQRGAALRQALQRGQPVTRAAVDAGFNSPSRLYASVSRELGMAPSAFRRQ
HCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHC
GAGLRIDYATAPTSLGTVLVAATDKGICRIAFGDEPAALVHELKASFARAELVESPARLA
CCCEEEEECCCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHH
PFIAQIGAYLDGTRHTFDLPLDIAPTAFQQRVWEALTHIPYGETRSYSEIAAALGAPGAV
HHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHH
RAVASACASNPVALAIPCHRVVQKGGALAGYRWGVRRKATLLASEARRARDDAPATAAIE
HHHHHHHCCCCEEEEECHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHHCCCCCCEEECC
EDSTA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]