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The map label for this gene is rlmI [H]

Identifier: 161526252

GI number: 161526252

Start: 3365948

End: 3367162

Strand: Direct

Name: rlmI [H]

Synonym: Bmul_3083

Alternate gene names: 161526252

Gene position: 3365948-3367162 (Clockwise)

Preceding gene: 161526251

Following gene: 161526253

Centisome position: 97.61

GC content: 69.22

Gene sequence:

>1215_bases
ATGCAAACCGTCACACTGAAGCCGTCCAAGGAAAAATCGCTGCTGCGTCGCCATCCATGGATCTACGCGAACGCGATCGA
CCGCGTCGACGGCAAACCGGCGCCGGGCGCGACGGTCGTCGTGCGCGCGCACGACGGCCGCTTTCTCGCGCGCGGCGCGT
ACAGCCCGCAGTCGCAGATCCGCGTGCGCGTATGGAGCTTCGACGAGAACGAGCCGATCGATCACGCGTTCTTCAAGCGC
CGCGTGCAGCGCGCGGTGGCGCACCGCACCGCGATGGTGTCGGGCACCGGCGCCGTGCGCCTTGTATTCGGCGAGGCCGA
CGGGCTGCCGGGCCTGATCGTCGACCACTACGTGGCGGATACCGCCGAAGCCGGCGCCGCGCCGCGCGGCCAGCTCGTCT
GCCAGTTCATGGCGGCGGGCGTCGAAGCGTGGAAGGACGCGATCGTCGCGGCGCTGGTCGGCGCGACCGGCTGCCCGAAC
GTCTACGAGCGCTCGGACGTGTCGATCCGCGACAAGGAAGGGCTCGAACAGACGACCGGCGTGCTCGCCGGCGACGCGCC
GCCCGCGACGCTGATCGTCGACGAAAACGGCGTGCGCTATCACGTCGACGTGCCGAACGGCCACAAGACCGGCTTCTACG
TCGATCAGCGCGACAACCGCGCGCTCGTCGCGCAGCATGCGGCGGGCCGCGACGTGCTGAACTGCTTCTGCTACACGGGC
GGCTTTTCGCTGGCGGCGCTCAAGGGCGGCGCGACGCGCGTCGTGTCGATCGACTCGTCCGGCGACGCGCTCGCGCTCGC
GCAGCAGAACGTCGTCGCGAACGGCTTCGATCCCGCGCGCGCGACCTGGCTCGACGCCGATGCGTTCAAGACGCTGCGCC
GCCTCGTCGACGAAGGCGAGCGCTTCGACCTGATCGTGCTCGATCCGCCGAAATTCGCGCCGACGCGCGACAGCGTGGAC
CGCGCCGCACGCGCGTACAAGGACATCAACCTGAGCGGGTTCCGCCTGCTGCGTCCGGGCGGCCTGCTGTTCACCTATTC
GTGCTCGGGCGCGATCGACATGGATCTGTTCCAGAAGATCGTCGCGGGCGCGGCGGCCGACGCGAAAGTCGACGCGCGGA
TCCTGAAGCGCTTGGGCGCGGGCGTCGATCACCCGCTGCTCGCCGCGTTCCCGGAAGGCGAATATTTGAAGGGGCTGCTG
TTGCAAGTCGTGTAA

Upstream 100 bases:

>100_bases
CCGCGCGCGAAGAAGCGCGACTGACGCGCTTTCCGTGTTATCCGCCGCGCGCGGCGACCGGCGACGGTCGGCTCCGCCCG
CGGCCCATTTCGAATTCATC

Downstream 100 bases:

>100_bases
GCGCCCCGATCTTCGCGCAGGGCGTGCGCCGCGGCAGGCCCGGCCGGCCGCGGCGCGCCACCCGCCGCCCGCTGCGCGCC
ACGCAGCCCGACCTCGCGAC

Product: PUA domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQTVTLKPSKEKSLLRRHPWIYANAIDRVDGKPAPGATVVVRAHDGRFLARGAYSPQSQIRVRVWSFDENEPIDHAFFKR
RVQRAVAHRTAMVSGTGAVRLVFGEADGLPGLIVDHYVADTAEAGAAPRGQLVCQFMAAGVEAWKDAIVAALVGATGCPN
VYERSDVSIRDKEGLEQTTGVLAGDAPPATLIVDENGVRYHVDVPNGHKTGFYVDQRDNRALVAQHAAGRDVLNCFCYTG
GFSLAALKGGATRVVSIDSSGDALALAQQNVVANGFDPARATWLDADAFKTLRRLVDEGERFDLIVLDPPKFAPTRDSVD
RAARAYKDINLSGFRLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAGVDHPLLAAFPEGEYLKGLL
LQVV

Sequences:

>Translated_404_residues
MQTVTLKPSKEKSLLRRHPWIYANAIDRVDGKPAPGATVVVRAHDGRFLARGAYSPQSQIRVRVWSFDENEPIDHAFFKR
RVQRAVAHRTAMVSGTGAVRLVFGEADGLPGLIVDHYVADTAEAGAAPRGQLVCQFMAAGVEAWKDAIVAALVGATGCPN
VYERSDVSIRDKEGLEQTTGVLAGDAPPATLIVDENGVRYHVDVPNGHKTGFYVDQRDNRALVAQHAAGRDVLNCFCYTG
GFSLAALKGGATRVVSIDSSGDALALAQQNVVANGFDPARATWLDADAFKTLRRLVDEGERFDLIVLDPPKFAPTRDSVD
RAARAYKDINLSGFRLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAGVDHPLLAAFPEGEYLKGLL
LQVV
>Mature_404_residues
MQTVTLKPSKEKSLLRRHPWIYANAIDRVDGKPAPGATVVVRAHDGRFLARGAYSPQSQIRVRVWSFDENEPIDHAFFKR
RVQRAVAHRTAMVSGTGAVRLVFGEADGLPGLIVDHYVADTAEAGAAPRGQLVCQFMAAGVEAWKDAIVAALVGATGCPN
VYERSDVSIRDKEGLEQTTGVLAGDAPPATLIVDENGVRYHVDVPNGHKTGFYVDQRDNRALVAQHAAGRDVLNCFCYTG
GFSLAALKGGATRVVSIDSSGDALALAQQNVVANGFDPARATWLDADAFKTLRRLVDEGERFDLIVLDPPKFAPTRDSVD
RAARAYKDINLSGFRLLRPGGLLFTYSCSGAIDMDLFQKIVAGAAADAKVDARILKRLGAGVDHPLLAAFPEGEYLKGLL
LQVV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=403, Percent_Identity=46.1538461538462, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787181, Length=169, Percent_Identity=31.9526627218935, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43489; Mature: 43489

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVTLKPSKEKSLLRRHPWIYANAIDRVDGKPAPGATVVVRAHDGRFLARGAYSPQSQI
CCEEECCCCHHHHHHHHCCCEEEEHHHCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCE
RVRVWSFDENEPIDHAFFKRRVQRAVAHRTAMVSGTGAVRLVFGEADGLPGLIVDHYVAD
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCCCCCHHHHHHHHH
TAEAGAAPRGQLVCQFMAAGVEAWKDAIVAALVGATGCPNVYERSDVSIRDKEGLEQTTG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHC
VLAGDAPPATLIVDENGVRYHVDVPNGHKTGFYVDQRDNRALVAQHAAGRDVLNCFCYTG
EEECCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEEECCCCHHHHEEEEECC
GFSLAALKGGATRVVSIDSSGDALALAQQNVVANGFDPARATWLDADAFKTLRRLVDEGE
CCEEEEECCCCEEEEEECCCCCEEEEECCCCEECCCCCCHHEEECHHHHHHHHHHHCCCC
RFDLIVLDPPKFAPTRDSVDRAARAYKDINLSGFRLLRPGGLLFTYSCSGAIDMDLFQKI
CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHH
VAGAAADAKVDARILKRLGAGVDHPLLAAFPEGEYLKGLLLQVV
HHCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MQTVTLKPSKEKSLLRRHPWIYANAIDRVDGKPAPGATVVVRAHDGRFLARGAYSPQSQI
CCEEECCCCHHHHHHHHCCCEEEEHHHCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCE
RVRVWSFDENEPIDHAFFKRRVQRAVAHRTAMVSGTGAVRLVFGEADGLPGLIVDHYVAD
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCEEEEEEECCCCCCCHHHHHHHHH
TAEAGAAPRGQLVCQFMAAGVEAWKDAIVAALVGATGCPNVYERSDVSIRDKEGLEQTTG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHC
VLAGDAPPATLIVDENGVRYHVDVPNGHKTGFYVDQRDNRALVAQHAAGRDVLNCFCYTG
EEECCCCCEEEEECCCCCEEEEECCCCCCCCEEEECCCCCEEEEECCCCHHHHEEEEECC
GFSLAALKGGATRVVSIDSSGDALALAQQNVVANGFDPARATWLDADAFKTLRRLVDEGE
CCEEEEECCCCEEEEEECCCCCEEEEECCCCEECCCCCCHHEEECHHHHHHHHHHHCCCC
RFDLIVLDPPKFAPTRDSVDRAARAYKDINLSGFRLLRPGGLLFTYSCSGAIDMDLFQKI
CEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHH
VAGAAADAKVDARILKRLGAGVDHPLLAAFPEGEYLKGLLLQVV
HHCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA