| Definition | Burkholderia multivorans ATCC 17616 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010084 |
| Length | 3,448,466 |
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The map label for this gene is murE [H]
Identifier: 161526010
GI number: 161526010
Start: 3096207
End: 3097748
Strand: Reverse
Name: murE [H]
Synonym: Bmul_2841
Alternate gene names: 161526010
Gene position: 3097748-3096207 (Counterclockwise)
Preceding gene: 161526011
Following gene: 161526009
Centisome position: 89.83
GC content: 71.4
Gene sequence:
>1542_bases ATGAGCGCCGCTCGCAGTTCCCATCCGGCGCATCAGCAAATCGCAGCCGCGCTTGCGTGGCTGCGTCAGCATGTAGCCCC CGCCGCGCAACTGCATGCGGACACGCGCAGCCTTCGGGCCGGCGACGTGTTCGTCGCCTATGCGGTCGACGGGGCCGACA ATCGCGCATTCATTGCCGACGCGCTCGCGCGCGGCGCGGCCGCCGTGCTGTATCAGCCCGAAGGACTCGCCGCCACGCCG AACGCGCCCGTCGCGCTCGCGGTGCCGGCGCTCGACCAGCTCGCCGGCGAGATTGCGAGCGGCTGGTACGGCGATCCGAG CGACGGCCTGCTCGCGATCGGCGTGACCGGCACGAACGGCAAGACGTCGTGCACGCAGTGGATCGCCGCCGCGCTGACGG CGCTGCACCAGCCGTGCGCCATCATCGGCACGCTCGGCAGCGGAATGCCGGGCCACCTCGTGCCGACCGGCTTCACGACG CCCGATGCGCCGCAGCTGCAGCGCAGCCTCGCGCAGCTGCGCGACGGCGGCGCGCAGGCGGTCGCGATGGAAGTGTCGTC GCACGCGCTGCATCAGGGGCGCGTGAACGGCACGGCGTTCGACATCGCCGTCTTCACGAATCTGACGCAGGATCACCTCG ACTATCACCGCACGTTCGAAGCGTACGAAGCCGCGAAGGCGAAGCTGTTCGCCTGGCGCGGACTGCGTGCGGCGGTCGTG AACCGCGACGACGCGGCCGGCCGCCGCCTGCTCGAAAAACTCGCGGGCCGCGTGCGCACGATCGCGTACGGAATCGGCGA TGCGCAGCAGGCGCCCGACGCCGATCGCGAACTGGTCGCGATCGATGTGCGCGCGACCGCGACCGGCACGGCATTCCGTC TGCGTTCGTCGTGGGGCGACGCGGACGTCGAGGTCGGCACGCTCGGCACGTTCAATGTCAGCAACCTGCTCGCCGTGCTC GGCGCGCTGCTCGCCGCCGACGTGCCGTTCGACGCGGCGCTTGCCGAGATCGCGCGGCTCGAGCCCGTCAACGGCCGGAT GCAGCGTCTCGGCGGCCGGCTGCAGAACGACGAGCCGCTCGTCGTGATCGATTACGCACATACGCCCGACGCGCTCGAGA AGACGCTCGACGCGCTGCGCCCGATCGCCGTCGCGCGCGGCGGCCGGCTGATCTGCATGTTCGGCTGCGGCGGCGATCGC GATGCGACCAAGCGTCCCCTGATGGGCGCGATCGCCGAACGGCTCGCCGACGACGTCGTCGTCACGAGCGACAACCCGCG CAGCGAGGATCCGCAAGCGATCATCGATCAGATCGTCGCCGGCATGACGGCGCCCGATCGCGCGCGCCGCATCGAGGATC GCGCGAGCGCGATCCTGCAGGCCGTGCGCGGCGCGGCGCGCGAGGACGTCGTCGTGCTCGCCGGCAAGGGACACGAGGCA ACGCAGGAAATCATGGGCAAGAAGCGCGCATTCTCCGACCAGGACCACGCGCGGCTCGCGCTCGCGGCGCGCGCGACGCA CGGCAAGGGAGGCGGCGAATGA
Upstream 100 bases:
>100_bases CCGCCGCGCCTTCGGCCGGTGCGCAAAAGCTGGCCGCGGGCGGCAAGGCGAAGCATATGATCGTATCCAGCACCACACGC AATTCACCAGGAGTCGTTCG
Downstream 100 bases:
>100_bases CGATGCTGAGTCTCGACGAAGCCGCGCGCCTGATTCCCGGCGCGACGGTTCATGGCGATGCGCACGTGACGTTCGAGCGC GTGTCGACCGACAGCCGCAC
Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase
Products: NA
Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]
Number of amino acids: Translated: 513; Mature: 512
Protein sequence:
>513_residues MSAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFVAYAVDGADNRAFIADALARGAAAVLYQPEGLAATP NAPVALAVPALDQLAGEIASGWYGDPSDGLLAIGVTGTNGKTSCTQWIAAALTALHQPCAIIGTLGSGMPGHLVPTGFTT PDAPQLQRSLAQLRDGGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRAAVV NRDDAAGRRLLEKLAGRVRTIAYGIGDAQQAPDADRELVAIDVRATATGTAFRLRSSWGDADVEVGTLGTFNVSNLLAVL GALLAADVPFDAALAEIARLEPVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIAVARGGRLICMFGCGGDR DATKRPLMGAIAERLADDVVVTSDNPRSEDPQAIIDQIVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGHEA TQEIMGKKRAFSDQDHARLALAARATHGKGGGE
Sequences:
>Translated_513_residues MSAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFVAYAVDGADNRAFIADALARGAAAVLYQPEGLAATP NAPVALAVPALDQLAGEIASGWYGDPSDGLLAIGVTGTNGKTSCTQWIAAALTALHQPCAIIGTLGSGMPGHLVPTGFTT PDAPQLQRSLAQLRDGGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRAAVV NRDDAAGRRLLEKLAGRVRTIAYGIGDAQQAPDADRELVAIDVRATATGTAFRLRSSWGDADVEVGTLGTFNVSNLLAVL GALLAADVPFDAALAEIARLEPVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIAVARGGRLICMFGCGGDR DATKRPLMGAIAERLADDVVVTSDNPRSEDPQAIIDQIVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGHEA TQEIMGKKRAFSDQDHARLALAARATHGKGGGE >Mature_512_residues SAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFVAYAVDGADNRAFIADALARGAAAVLYQPEGLAATPN APVALAVPALDQLAGEIASGWYGDPSDGLLAIGVTGTNGKTSCTQWIAAALTALHQPCAIIGTLGSGMPGHLVPTGFTTP DAPQLQRSLAQLRDGGAQAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRAAVVN RDDAAGRRLLEKLAGRVRTIAYGIGDAQQAPDADRELVAIDVRATATGTAFRLRSSWGDADVEVGTLGTFNVSNLLAVLG ALLAADVPFDAALAEIARLEPVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIAVARGGRLICMFGCGGDRD ATKRPLMGAIAERLADDVVVTSDNPRSEDPQAIIDQIVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGHEAT QEIMGKKRAFSDQDHARLALAARATHGKGGGE
Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]
COG id: COG0769
COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family. MurE subfamily [H]
Homologues:
Organism=Escherichia coli, GI1786273, Length=470, Percent_Identity=44.6808510638298, Blast_Score=315, Evalue=6e-87,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR005761 [H]
Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.13 [H]
Molecular weight: Translated: 53854; Mature: 53723
Theoretical pI: Translated: 6.51; Mature: 6.51
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 2.3 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFVAYAVDGADNRAFIAD CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHH ALARGAAAVLYQPEGLAATPNAPVALAVPALDQLAGEIASGWYGDPSDGLLAIGVTGTNG HHHCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC KTSCTQWIAAALTALHQPCAIIGTLGSGMPGHLVPTGFTTPDAPQLQRSLAQLRDGGAQA CHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCEE VAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRAAVV EEEEHHHHHHHCCCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE NRDDAAGRRLLEKLAGRVRTIAYGIGDAQQAPDADRELVAIDVRATATGTAFRLRSSWGD CCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCC ADVEVGTLGTFNVSNLLAVLGALLAADVPFDAALAEIARLEPVNGRMQRLGGRLQNDEPL CCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCE VVIDYAHTPDALEKTLDALRPIAVARGGRLICMFGCGGDRDATKRPLMGAIAERLADDVV EEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCEE VTSDNPRSEDPQAIIDQIVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGHEA EECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHH TQEIMGKKRAFSDQDHARLALAARATHGKGGGE HHHHHHHHHCCCCCCHHEEEEEEECCCCCCCCC >Mature Secondary Structure SAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLRAGDVFVAYAVDGADNRAFIAD CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHH ALARGAAAVLYQPEGLAATPNAPVALAVPALDQLAGEIASGWYGDPSDGLLAIGVTGTNG HHHCCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC KTSCTQWIAAALTALHQPCAIIGTLGSGMPGHLVPTGFTTPDAPQLQRSLAQLRDGGAQA CHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCEE VAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRAAVV EEEEHHHHHHHCCCCCCEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE NRDDAAGRRLLEKLAGRVRTIAYGIGDAQQAPDADRELVAIDVRATATGTAFRLRSSWGD CCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCC ADVEVGTLGTFNVSNLLAVLGALLAADVPFDAALAEIARLEPVNGRMQRLGGRLQNDEPL CCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCE VVIDYAHTPDALEKTLDALRPIAVARGGRLICMFGCGGDRDATKRPLMGAIAERLADDVV EEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCEE VTSDNPRSEDPQAIIDQIVAGMTAPDRARRIEDRASAILQAVRGAAREDVVVLAGKGHEA EECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHH TQEIMGKKRAFSDQDHARLALAARATHGKGGGE HHHHHHHHHCCCCCCHHEEEEEEECCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11823852 [H]