| Definition | Burkholderia multivorans ATCC 17616 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010084 |
| Length | 3,448,466 |
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The map label for this gene is murF [H]
Identifier: 161526009
GI number: 161526009
Start: 3094804
End: 3096210
Strand: Reverse
Name: murF [H]
Synonym: Bmul_2840
Alternate gene names: 161526009
Gene position: 3096210-3094804 (Counterclockwise)
Preceding gene: 161526010
Following gene: 161526008
Centisome position: 89.79
GC content: 72.0
Gene sequence:
>1407_bases ATGACGATGCTGAGTCTCGACGAAGCCGCGCGCCTGATTCCCGGCGCGACGGTTCATGGCGATGCGCACGTGACGTTCGA GCGCGTGTCGACCGACAGCCGCACCGTCGGTGCGGGCGACCTGTTCGTCGCGCTGAAAGGCGAGCGCTTCGACGCGCACG ACTTTCTCGCGGACGTCGCTGCACGCGGCGCCGCGGCCGCCCTGGTGGCGCAGGCGCCGGCGGGCATCGCGCTGCCGATG ATCGTCGGCGGCGAAACGCGCGCGGCGCTCGGCGCGCTCGCGCACGGCTGGCGCAAACGATTCGCGCTGCCGGTCGTCGC GGTGACGGGCAGCAACGGCAAGACGACCGTGAAGGAAATGATCGCGTCGATCTTCGCCGCGGCGGTCGGCGCCCACGCAC GGCTCGCGACGGCCGGCAATCTGAACAACGACGTCGGCCTGCCGCTGACGCTGCTGCGCCTGGCGGCCGCGCACCGCCTC GCGGTGATCGAGCTCGGAATGAATCATCGGGGCGAAACGGAGATCCTCGCGCGCCTGACGGCGCCGACGGTCGCGCTCGT GAACAACGCACAGCGCGAGCACCAGGAGTTCATGGCGACGGTCGAAGCCGTCGCGCTCGAACATGCGGCCGTGATCCATG CGCTGCCGCCCGACGGCGTCGCGGTGTTTCCGGCCGACGATGCGTACGCGAGCATCTGGCGCGTCGCGGCGACCGGCAAC CGGATTCTCGATTTCGCGCTGCACGACGACGAACGGCAGACGGCGGCGCAAGTCACCGGCCGGCTGCACGGCGGCGAGCT CGCGATCGATACGCCGTCGGGCACGCTGACCGTGCGGCTGCGCGCGCTCGGCGAGCACAACGCGCGCAATGCGCTGGCCG CGACGGCCGCCGCGCTGGCCGCAGGCGTCGCGCTGTCGGCGATCAAGCAGGGCCTCGAATCGTTCGAACCGGTGAAGGGC CGGCTGCAGGTGCGTCGCGCGAGCGCGGGCAGCCTCGCCGGCGCGACGGTCGTCGACGATACGTACAACGCGAACCCCGA CTCGATGCGCGCGGCGATCGACGTGCTCGCTGCGCAACCGGCGCCGCGCGTGCTGGTGATCGGCGACATGGGCGAGGTCG GCGACGAAGGGCCGGCGTTTCACCGTGAGGTAGGCGCATACGCGCGCGAGCGCGGCATCGACGCGCTGTTCGCGCTCGGT GACGCATCGCGCGACGCCTGCGCGGCGTACGGCGAAGCGGCGCGCCATTTCGACGACGTCGGCACGCTCGTCGACGCGCT GCTCGCGGCGGGCTACGGCGCCGACGCGTCGGTGCTCGTGAAGGGCTCGCGGTACATGAAGATGGAGCGCGTGGTCGACG CGCTGACGAACCAACCCGCGGCGGGCACGACGCCCGCCGCCCACTGA
Upstream 100 bases:
>100_bases CAACGCAGGAAATCATGGGCAAGAAGCGCGCATTCTCCGACCAGGACCACGCGCGGCTCGCGCTCGCGGCGCGCGCGACG CACGGCAAGGGAGGCGGCGA
Downstream 100 bases:
>100_bases GTAGAAGGAAGGAAGCATGCTGCTGGCGCTGGCGCAATGGCTGCAAGGAGACGCAAGCTTTTTGCGCTTGTTCACGTACC TGACGTTCCGTGCCGTGATG
Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 468; Mature: 467
Protein sequence:
>468_residues MTMLSLDEAARLIPGATVHGDAHVTFERVSTDSRTVGAGDLFVALKGERFDAHDFLADVAARGAAAALVAQAPAGIALPM IVGGETRAALGALAHGWRKRFALPVVAVTGSNGKTTVKEMIASIFAAAVGAHARLATAGNLNNDVGLPLTLLRLAAAHRL AVIELGMNHRGETEILARLTAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYASIWRVAATGN RILDFALHDDERQTAAQVTGRLHGGELAIDTPSGTLTVRLRALGEHNARNALAATAAALAAGVALSAIKQGLESFEPVKG RLQVRRASAGSLAGATVVDDTYNANPDSMRAAIDVLAAQPAPRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALG DASRDACAAYGEAARHFDDVGTLVDALLAAGYGADASVLVKGSRYMKMERVVDALTNQPAAGTTPAAH
Sequences:
>Translated_468_residues MTMLSLDEAARLIPGATVHGDAHVTFERVSTDSRTVGAGDLFVALKGERFDAHDFLADVAARGAAAALVAQAPAGIALPM IVGGETRAALGALAHGWRKRFALPVVAVTGSNGKTTVKEMIASIFAAAVGAHARLATAGNLNNDVGLPLTLLRLAAAHRL AVIELGMNHRGETEILARLTAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYASIWRVAATGN RILDFALHDDERQTAAQVTGRLHGGELAIDTPSGTLTVRLRALGEHNARNALAATAAALAAGVALSAIKQGLESFEPVKG RLQVRRASAGSLAGATVVDDTYNANPDSMRAAIDVLAAQPAPRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALG DASRDACAAYGEAARHFDDVGTLVDALLAAGYGADASVLVKGSRYMKMERVVDALTNQPAAGTTPAAH >Mature_467_residues TMLSLDEAARLIPGATVHGDAHVTFERVSTDSRTVGAGDLFVALKGERFDAHDFLADVAARGAAAALVAQAPAGIALPMI VGGETRAALGALAHGWRKRFALPVVAVTGSNGKTTVKEMIASIFAAAVGAHARLATAGNLNNDVGLPLTLLRLAAAHRLA VIELGMNHRGETEILARLTAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYASIWRVAATGNR ILDFALHDDERQTAAQVTGRLHGGELAIDTPSGTLTVRLRALGEHNARNALAATAAALAAGVALSAIKQGLESFEPVKGR LQVRRASAGSLAGATVVDDTYNANPDSMRAAIDVLAAQPAPRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALGD ASRDACAAYGEAARHFDDVGTLVDALLAAGYGADASVLVKGSRYMKMERVVDALTNQPAAGTTPAAH
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=438, Percent_Identity=43.1506849315069, Blast_Score=274, Evalue=1e-74,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 48374; Mature: 48243
Theoretical pI: Translated: 6.20; Mature: 6.20
Prosite motif: PS00211 ABC_TRANSPORTER_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTMLSLDEAARLIPGATVHGDAHVTFERVSTDSRTVGAGDLFVALKGERFDAHDFLADVA CCEECHHHHHHHCCCCEECCCCEEEEEEECCCCCEECCCEEEEEEECCCCCHHHHHHHHH ARGAAAALVAQAPAGIALPMIVGGETRAALGALAHGWRKRFALPVVAVTGSNGKTTVKEM HCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH IASIFAAAVGAHARLATAGNLNNDVGLPLTLLRLAAAHRLAVIELGMNHRGETEILARLT HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHC APTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYASIWRVAATGN CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCHHHHHHHHHEECCC RILDFALHDDERQTAAQVTGRLHGGELAIDTPSGTLTVRLRALGEHNARNALAATAAALA EEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHH AGVALSAIKQGLESFEPVKGRLQVRRASAGSLAGATVVDDTYNANPDSMRAAIDVLAAQP HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC APRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALGDASRDACAAYGEAARHFDDV CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHH GTLVDALLAAGYGADASVLVKGSRYMKMERVVDALTNQPAAGTTPAAH HHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC >Mature Secondary Structure TMLSLDEAARLIPGATVHGDAHVTFERVSTDSRTVGAGDLFVALKGERFDAHDFLADVA CEECHHHHHHHCCCCEECCCCEEEEEEECCCCCEECCCEEEEEEECCCCCHHHHHHHHH ARGAAAALVAQAPAGIALPMIVGGETRAALGALAHGWRKRFALPVVAVTGSNGKTTVKEM HCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH IASIFAAAVGAHARLATAGNLNNDVGLPLTLLRLAAAHRLAVIELGMNHRGETEILARLT HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHC APTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYASIWRVAATGN CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCHHHHHHHHHEECCC RILDFALHDDERQTAAQVTGRLHGGELAIDTPSGTLTVRLRALGEHNARNALAATAAALA EEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHH AGVALSAIKQGLESFEPVKGRLQVRRASAGSLAGATVVDDTYNANPDSMRAAIDVLAAQP HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCC APRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALGDASRDACAAYGEAARHFDDV CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHH GTLVDALLAAGYGADASVLVKGSRYMKMERVVDALTNQPAAGTTPAAH HHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]