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The map label for this gene is penA [H]

Identifier: 161526011

GI number: 161526011

Start: 3097745

End: 3099595

Strand: Reverse

Name: penA [H]

Synonym: Bmul_2842

Alternate gene names: 161526011

Gene position: 3099595-3097745 (Counterclockwise)

Preceding gene: 161526012

Following gene: 161526010

Centisome position: 89.88

GC content: 66.45

Gene sequence:

>1851_bases
ATGAAGAAGCCTCCGCAGAAGCGCCACGACGTGAAGTTCTCGTCGAGCCCCGTGCTGTCCGTGCATCTGCCGATGTGGCG
CTCGAAGCTCGTCGTGTTCATGCTGTTCATGGCGTTCGTCGCGCTCGCGGCCCGCGCCTTCTGGATCCAGGGGCCCGGCA
ACGCGTTCTATCAGAAGCAGGGCGAAAGCCGGTATCAGCGCACGATCGAGCTGCCGGCCACGCGCGGCAAGATCCTCGAC
CGCAACGGGCTCGTGCTCGCGACGAGCCTGCCCGTGCGCGCGATCTGGGCGATTCCGGACGCGGTGCCCGACGACCTCGA
CGCCGACAAGATCAACCAGCTCGGCAAGCTGCTCGGCATGACGCCGAAGGAGCTGCGCGTCAAGCTGTCGGAAGACAAGG
GCTTCGTCTACGTGAAGCGCCAGGTGCCGATCGACGTCGCGGACAAGGTCGCCGCGCTCGACATTCCCGGCATCTATCAG
CGCAACGAATACAAGCGCTTCTATCCGGAAGGCGAGATCACCGCGCACCTGATCGGCTTCACGAACGTCGAGGACGAAGG
GCAGGAGGGCGTCGAGCTCGGCGACCAGAAGCTGCTGTCGGGCACGTCCGGCATGCGCCGCGTGATCAAGGACCGTCTCG
GCCACATCGTCGAGGACGTCGCCGAGCAGATTCCGCCGCACAACGGCACGGACGTCGATCTGTCGATCGACAGCAAGATC
CAGTACATCGCATACGCGAACCTGAAGGCCGCCGTCGAGAAGTTCAAGGCGAAGGCCGGCGCGGCGATGGTCGTCGACGT
GCGTACCGGCGAAGTGCTCGCGCTCGTCAACTATCCGACGTACAACCCGAACGACCGCTCGCGGCTGACCGGCGAGCAAC
TGCGCAACCGGATCCTGACCGACGTGTTCGAGCCGGGCTCGATCATGAAGCCGTTTACGGTGTCGCTCGCGCTCGACCTG
CACCGCGTGACGCCGAACACGCTCGTCGAAACGGGCAACGGGCATTTCGTGCTCGACGGTGCGCCGATCACCGACGATGC
CGGTTTCGGCACGCTGACGGTCGGCGGCGTGATCCAGAAGTCGAGCAACATCGGCGCGACGAAGATCGCGATGACGATGC
GGCCCGAGGAAATGTGGAACATGTATACGAGCATCGGCCTCGGCCAGGCGCCGAAGGTCGGCTTCCCGGGCGCGGCCGCG
GGCCGGCTGCGTCCGTGGAAGAGCTGGCGCCGCATCGAGCAGGCGACGATGTCGTACGGCTACGGGCTGTCCGCCTCGCT
GTTCCAGCTCGCGCGCGCCTACACGGCGATCGCGCACGACGGCGAACTGATGCCCGTGACCATTTTCAAGACCGACCCCA
AGCAGCCGGTCGCCGGTACGCAGGTGTTTACGCCGACCACCGCGCGCGAAGTGCGGGCGATGCTCGAGACCGTGGTCGCG
CCGGGCGGCACGTCGCCGGATGCGGCGGTGCCCGGCTATCGCGTCGGCGGCAAGAGCGGTACCGCGTACAAGCATGAAGG
CCACGGCTATACGCGCAAGTACCGCGCGTCGTTCGTCGGGATGGCGCCGATGCCGAATCCGCGGATCGTCGTCGCGGTGT
CGGTCGACGAGCCGACAGCCGGCAGCCACTTCGGCGGACAGGTGTCCGGCCCCGTCTTCTCGGCGATCGCCGGCGATACG
CTGCGCGCGCTGAACGTGCCGCCGAACATGCCGATCAAGCAGCTCGTCGTGTCGGACGATTCGCCGGCCGCCGCGCCTTC
GGCCGGTGCGCAAAAGCTGGCCGCGGGCGGCAAGGCGAAGCATATGATCGTATCCAGCACCACACGCAATTCACCAGGAG
TCGTTCGATGA

Upstream 100 bases:

>100_bases
GCACGCAGTATCTGACGCTGCCGCCGGGCGCCGCGAAGGCGGTCGACGCGCCGATCCCCGCGTCGGCCGACTCGGCCGGC
AAGGGCAAGGGAGGCGCGCG

Downstream 100 bases:

>100_bases
GCGCCGCTCGCAGTTCCCATCCGGCGCATCAGCAAATCGCAGCCGCGCTTGCGTGGCTGCGTCAGCATGTAGCCCCCGCC
GCGCAACTGCATGCGGACAC

Product: peptidoglycan glycosyltransferase

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MKKPPQKRHDVKFSSSPVLSVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYQKQGESRYQRTIELPATRGKILD
RNGLVLATSLPVRAIWAIPDAVPDDLDADKINQLGKLLGMTPKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQ
RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLSGTSGMRRVIKDRLGHIVEDVAEQIPPHNGTDVDLSIDSKI
QYIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRLTGEQLRNRILTDVFEPGSIMKPFTVSLALDL
HRVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQKSSNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAAA
GRLRPWKSWRRIEQATMSYGYGLSASLFQLARAYTAIAHDGELMPVTIFKTDPKQPVAGTQVFTPTTAREVRAMLETVVA
PGGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSAIAGDT
LRALNVPPNMPIKQLVVSDDSPAAAPSAGAQKLAAGGKAKHMIVSSTTRNSPGVVR

Sequences:

>Translated_616_residues
MKKPPQKRHDVKFSSSPVLSVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYQKQGESRYQRTIELPATRGKILD
RNGLVLATSLPVRAIWAIPDAVPDDLDADKINQLGKLLGMTPKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQ
RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLSGTSGMRRVIKDRLGHIVEDVAEQIPPHNGTDVDLSIDSKI
QYIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRLTGEQLRNRILTDVFEPGSIMKPFTVSLALDL
HRVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQKSSNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAAA
GRLRPWKSWRRIEQATMSYGYGLSASLFQLARAYTAIAHDGELMPVTIFKTDPKQPVAGTQVFTPTTAREVRAMLETVVA
PGGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSAIAGDT
LRALNVPPNMPIKQLVVSDDSPAAAPSAGAQKLAAGGKAKHMIVSSTTRNSPGVVR
>Mature_616_residues
MKKPPQKRHDVKFSSSPVLSVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYQKQGESRYQRTIELPATRGKILD
RNGLVLATSLPVRAIWAIPDAVPDDLDADKINQLGKLLGMTPKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQ
RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKLLSGTSGMRRVIKDRLGHIVEDVAEQIPPHNGTDVDLSIDSKI
QYIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRLTGEQLRNRILTDVFEPGSIMKPFTVSLALDL
HRVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQKSSNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAAA
GRLRPWKSWRRIEQATMSYGYGLSASLFQLARAYTAIAHDGELMPVTIFKTDPKQPVAGTQVFTPTTAREVRAMLETVVA
PGGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSAIAGDT
LRALNVPPNMPIKQLVVSDDSPAAAPSAGAQKLAAGGKAKHMIVSSTTRNSPGVVR

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=576, Percent_Identity=40.7986111111111, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786854, Length=603, Percent_Identity=24.3781094527363, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 66626; Mature: 66626

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPPQKRHDVKFSSSPVLSVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYQKQ
CCCCCCCHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCC
GESRYQRTIELPATRGKILDRNGLVLATSLPVRAIWAIPDAVPDDLDADKINQLGKLLGM
CCCHHHEEEECCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCC
TPKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQRNEYKRFYPEGEITAHLIGF
CHHHHEEEEECCCCEEEEEEECCCCHHCCEEEECCCCCCCCCCCHHCCCCCCEEEEEEEE
TNVEDEGQEGVELGDQKLLSGTSGMRRVIKDRLGHIVEDVAEQIPPHNGTDVDLSIDSKI
CCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCE
QYIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRLTGEQLRNRILT
EEEEEHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCHHCCHHHHHHHHHH
DVFEPGSIMKPFTVSLALDLHRVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQK
HHCCCCCCCCCEEEEEEEEEEECCCCCEEECCCCEEEECCCCCCCCCCCCEEEECCEEEC
SSNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYG
CCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
YGLSASLFQLARAYTAIAHDGELMPVTIFKTDPKQPVAGTQVFTPTTAREVRAMLETVVA
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHC
PGGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTA
CCCCCCCCCCCCEEECCCCCCCEEECCCCCCHHHHHHEEECCCCCCCCEEEEEEECCCCC
GSHFGGQVSGPVFSAIAGDTLRALNVPPNMPIKQLVVSDDSPAAAPSAGAQKLAAGGKAK
CCCCCCCCCCHHHHHHCCCCEEEECCCCCCCHHHEEECCCCCCCCCCCCHHHHHCCCCCC
HMIVSSTTRNSPGVVR
EEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKPPQKRHDVKFSSSPVLSVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYQKQ
CCCCCCCHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCC
GESRYQRTIELPATRGKILDRNGLVLATSLPVRAIWAIPDAVPDDLDADKINQLGKLLGM
CCCHHHEEEECCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCC
TPKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQRNEYKRFYPEGEITAHLIGF
CHHHHEEEEECCCCEEEEEEECCCCHHCCEEEECCCCCCCCCCCHHCCCCCCEEEEEEEE
TNVEDEGQEGVELGDQKLLSGTSGMRRVIKDRLGHIVEDVAEQIPPHNGTDVDLSIDSKI
CCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCE
QYIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRLTGEQLRNRILT
EEEEEHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCHHCCHHHHHHHHHH
DVFEPGSIMKPFTVSLALDLHRVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQK
HHCCCCCCCCCEEEEEEEEEEECCCCCEEECCCCEEEECCCCCCCCCCCCEEEECCEEEC
SSNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYG
CCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
YGLSASLFQLARAYTAIAHDGELMPVTIFKTDPKQPVAGTQVFTPTTAREVRAMLETVVA
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHC
PGGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTA
CCCCCCCCCCCCEEECCCCCCCEEECCCCCCHHHHHHEEECCCCCCCCEEEEEEECCCCC
GSHFGGQVSGPVFSAIAGDTLRALNVPPNMPIKQLVVSDDSPAAAPSAGAQKLAAGGKAK
CCCCCCCCCCHHHHHHCCCCEEEECCCCCCCHHHEEECCCCCCCCCCCCHHHHHCCCCCC
HMIVSSTTRNSPGVVR
EEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]