Click here to switch to the map view.

The map label for this gene is glnG [H]

Identifier: 161524299

GI number: 161524299

Start: 1233763

End: 1235277

Strand: Direct

Name: glnG [H]

Synonym: Bmul_1124

Alternate gene names: 161524299

Gene position: 1233763-1235277 (Clockwise)

Preceding gene: 161524298

Following gene: 161524300

Centisome position: 35.78

GC content: 69.5

Gene sequence:

>1515_bases
ATGAAGCCGATCTGGATAGTAGACGACGATCAATCGATCCGCTGGGTGCTCGAGAAGGCGCTCGCGCGCGACAGCTTCGC
GACGAAGAGCTTCGCGAACGTGCGCGACGCGCTCGCCGCGCTCGATCACGAGACGCCGCAGGTGCTGGTATCCGACATCC
GGATGCCGGGCGGGTCCGGGCTCGAGCTGCTGCAGGCCGTGCACGAGCGCCTGCCGGGGCTGCCCGTCATCATCATGACT
GCGTTCTCCGATCTCGACAGCGCGGTCGCGGCCTTCCAGGGCGGCGCGTTCGAATATCTCGCGAAGCCGTTCGACGTCGA
CAAGGCCGTCGAGCTGATTCGCCGCGCGGTCGAAGAGAGCCTGCGCGGCGGCACGCCGCAGGACGACCGCGTCGCCGAGG
CGCCCGAGATGCTCGGCCAGGCACCCGCGATGCAGGACATGTTTCGCGCGATCGGCCGGCTGTCGCATTCGGCCGCGACC
GTGCTGATCACCGGCGAATCGGGCACCGGCAAGGAGCTCGTCGCGCGTGCGCTGCACCGTCATAGCCCGCGTGCGAACGG
CCCGTTCATCGCGCTGAACACGGCCGCGATTCCGAAGGATCTGCTCGAATCGGAACTGTTCGGCCACGAGCGCGGCGCGT
TCACGGGCGCGCAGACGACGCGGCAGGGCCGCTTCGAGCAGGCCGAGAACGGCACGCTGTTTCTCGACGAAATCGGCGAC
ATGCCGTTCGATCTGCAGACGCGGCTGCTGCGCGTGCTGTCCGACGGGCAGTTCTATCGCGTCGGCGGTCACAGTCCGCT
GCGCGCGAACGTACGCGTGATCGCCGCGACCCATCAGAACCTCGAGGCGCGCGTGCGGCAGGGGCTGTTCCGCGAAGACC
TCTATCACCGGCTGAACGTGATCCGGCTGCGGCTGCCGCCGCTGCGCGAGCGCAGCGAGGATATCCCGCTGCTCACGCGC
CATTTCCTGCAGAAGAGTGCGCGCGATCTCGGCGTCGAGCCGAAGCGCGTGTCGGACGAGGCGCTCGCGTATCTGACCTC
GCTGCCGTTTCCCGGCAACGTGCGCCAGCTCGAAAACCTCGCGAACTGGCTGACCGTGATGGCGCCCGCGCAGACGGTCG
AAATCAAGGATCTGCCGCCCGATCTCGTCCCGTCGGGCGCGCCGATCGCCGTATCGGCCGGCGACGGTGCGAACGCGCCC
GGCAGCCACGACGCGCCGGCCGCGCCGACGGTCGCCGCCGCGGCGCCGCTGGCATCGATGGCCGGCACGGCTGCGCCGAA
CGGTGCGCCTGCCGGCTACCCGATGTGGGAGCACGGGCTGCGCACCGAAGTCGCGCGATTGCTGCGCGAGAATTCGGCCG
GCGTGATGGACGAACTCGCGCGCCGCTTCGAGGCGGCCGTGATTCGCGAGGCGCTCGACTTCACGCGCGGCCGCAAGGTC
GAGGCCGCCGAGCGGCTCGGCATCGGCCGCAACACGATCACGCGCAAGATCCAGGAACTGCATCTGGAGCCCTGA

Upstream 100 bases:

>100_bases
ATCGAGGTCGAAAGCCGGCCGGGACGTACCGAATTTCAGATTCTGTTGCCGCTCGACGCATGAGCGGCGACATTGCGATA
CACCAATATCTGACCGACCT

Downstream 100 bases:

>100_bases
GCCTGCATCGCGGCATGCGCGGCGCCGCGCATGCCGCGATCGGACATAATCGCGCTTTGTGTTGCGCGCGCGATTCCGTC
ATGTCCGATTCCTTCCGCTG

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSGLELLQAVHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGTPQDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNVIRLRLPPLRERSEDIPLLTR
HFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVPSGAPIAVSAGDGANAP
GSHDAPAAPTVAAAAPLASMAGTAAPNGAPAGYPMWEHGLRTEVARLLRENSAGVMDELARRFEAAVIREALDFTRGRKV
EAAERLGIGRNTITRKIQELHLEP

Sequences:

>Translated_504_residues
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSGLELLQAVHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGTPQDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNVIRLRLPPLRERSEDIPLLTR
HFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVPSGAPIAVSAGDGANAP
GSHDAPAAPTVAAAAPLASMAGTAAPNGAPAGYPMWEHGLRTEVARLLRENSAGVMDELARRFEAAVIREALDFTRGRKV
EAAERLGIGRNTITRKIQELHLEP
>Mature_504_residues
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSGLELLQAVHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGTPQDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNVIRLRLPPLRERSEDIPLLTR
HFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVPSGAPIAVSAGDGANAP
GSHDAPAAPTVAAAAPLASMAGTAAPNGAPAGYPMWEHGLRTEVARLLRENSAGVMDELARRFEAAVIREALDFTRGRKV
EAAERLGIGRNTITRKIQELHLEP

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=501, Percent_Identity=56.2874251497006, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1788550, Length=506, Percent_Identity=39.1304347826087, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1790437, Length=392, Percent_Identity=42.3469387755102, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788905, Length=370, Percent_Identity=41.6216216216216, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082117, Length=225, Percent_Identity=53.7777777777778, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1789233, Length=380, Percent_Identity=38.1578947368421, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1789087, Length=291, Percent_Identity=43.9862542955326, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=48.936170212766, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1786524, Length=262, Percent_Identity=46.9465648854962, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=253, Percent_Identity=43.4782608695652, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=46.0176991150442, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081858, Length=239, Percent_Identity=34.3096234309623, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=36.283185840708, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789809, Length=111, Percent_Identity=29.7297297297297, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54892; Mature: 54892

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
LELLQAVHERLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGTPQDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIPLLTRHFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENL
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLVPSGAPIAVSAGDGANAPGSHDAPAAPTVAAAAPLASM
HHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
AGTAAPNGAPAGYPMWEHGLRTEVARLLRENSAGVMDELARRFEAAVIREALDFTRGRKV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAERLGIGRNTITRKIQELHLEP
HHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
LELLQAVHERLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGTPQDDRVAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIPLLTRHFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENL
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLVPSGAPIAVSAGDGANAPGSHDAPAAPTVAAAAPLASM
HHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
AGTAAPNGAPAGYPMWEHGLRTEVARLLRENSAGVMDELARRFEAAVIREALDFTRGRKV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAERLGIGRNTITRKIQELHLEP
HHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]