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The map label for this gene is ptrB [C]

Identifier: 161524300

GI number: 161524300

Start: 1235292

End: 1237457

Strand: Direct

Name: ptrB [C]

Synonym: Bmul_1125

Alternate gene names: 161524300

Gene position: 1235292-1237457 (Clockwise)

Preceding gene: 161524299

Following gene: 161524303

Centisome position: 35.82

GC content: 71.19

Gene sequence:

>2166_bases
ATGCGCGGCGCCGCGCATGCCGCGATCGGACATAATCGCGCTTTGTGTTGCGCGCGCGATTCCGTCATGTCCGATTCCTT
CCGCTGGCCCGCCGCGGCCGATCCCTTCCGTTTTCTCGAAGCGCTCGACAGCGCGCGCGCCCGCGCGTGGGTCGACGAGC
AGAACGCGCGCACGCGCGCCGCGCTGCGCGACGACGACGCGTACCGCACACTCGCCGCACGGCTCGCACGCGCGTATCTG
CCGCGTGAACGGCCGGTGATTCCGACGCGCTGGCGCGACTGGGCCTACGACCTGTGGCAGGACGATCTGCATCCGAAGGG
CCTCTGGCGTCGCGCGCGCTGGGACGACTGGCGTGCGGGCCGCCCCGAATGGAAGACGCTGCTCGACGTCGATGCGCTCG
GTGCGGCGGAAGGCGAATCGTGGGTGTTCGAGCAGGACGCGATCCTGTATCCGCACGGCGATCGCGCATTGCTGTCGCTG
TCGCCGGGCGGCGCGGATGCGGTCGTCGTCCGCGAATTCGATCTCGTGCTGCGACGCTTCGTCGACGACGGCTTCACGAT
CGACGTGCCGGGCCATCACACGGTCGGCTGGATCGATCGCGATACGGTGTACGTGAGCTGGGATCGCGGCGACGCGCATT
CGACCGCAGCCGGCTATCCGTACGACGTGCGGCGCTGGGTGCGCGGCACGCCGCTCGCGCAGGCGCCGGTCGTGTTTCGC
GGCGAACCGGACGACATCAGCGCCGGTGCGTGGTTCGATCCAATCGACGGTCGTCACGTCGCGTGGCGCAGCGTCGACTT
CTTCGACGCGCATACCTACCGGCTGACCGAGGCGGGCGAGTGGGCGCGCTACGACGTGCCGACGCACGTCGAGGTCGGCT
TCTGGGAAGGCTGGCTCGTGCTAGAGCCGCGGCTCGACTGGGACTGCGACGGCGTGCGTCATGCGGGCGGGTCGTTGCTC
GCGATCCGCGAGCGCGCGTTTCTCGACGGTTCGCGCGCGTTCACGACGCTGTTCGAGCCGCAGCCGTCGACGTCCGCCTG
CACGTGGACGCATACGCGCCACACGCTGATCGCGAGCTGGCTCGACGACGTGCACAACCGCACGCTGCTATGGGAGCCGC
GCGAGACGGCCGACGGTACGTGGACCTGGGATGCGCGGCCGCTCGATTGGCCGGGCGACGGGCAGATCGACGTCGAGCCC
GTCGAGCCGACGCTGAACGACGAGGTCTACGTCGACGTCGACACCTATCTCGATCCGCCCGAATGCTGGCTCGCCGACCT
TGCCGACCGCGCGGCGGATGCCGCGTCGCGGCGCGTGCTGCTCGACCGGCCGCCGGTGCAGTTCGATGCCGCCGGGCTCG
TCGTGCGTCGCGCGAGTGCACGCTCGCGCGACGGCACGCGCGTGCCGTACACGCTGATCGGCCCGCGCGATGCGCTCGAC
GGCGCTACGCGCGTCGCGCGGCCGTGCCTGTTGTCGGGCTATGGCGGCTTCGCGCTGCCGAACCTGCCCGGCTACAGCGA
CGCGCTCGGCATCGGCTGGCTCGAGCGCGGCGGCGTCGCGGCGTTCGCGCACATCCGCGGCGGCGGCGAATTCGGGCCGC
GCTGGCACGTCGACGCACAGCGCGAACATCGGCAACGTTCGTTCGACGATTTCATCGCGGTCGCGGAGGACCTGATCGCG
ACCGGCGTGACGACGGCCGCGCAGCTCGGCATCGAAGGCGGCAGCAACGGCGGGCTGCTGGTCGCCGCGTGCATGGTGCA
GCGCCCGGCGCTGTTCGGCGCGGTGCTGTGCCGCGTGCCGCTGCTCGACATGCGCCGCTATCCGAAGCTGCATGCGGGCG
CCGCATGGCTCGACGAATACGGCGACCCGGACGATCCCGACGAAGGGGCGGCGCTCGCCGCGTATTCGCCGTATCACCGC
GTGCGCGACGGCGTCGCGTATCCGCCGCTGCTGCTGACGACGTCGACGCGCGACGACCGCGTGCATCCGGCGCACGCGCG
CAAGATGGCCGCGCGCATGCAGGCGCTCGGTCATCGCAACGTGTGGTACTGGGAGAACACCGACGGCGGTCACGGCAGCG
CCGACGATCTGGAGCGCGCCGAATCCGACGCGGCCGAATTCGGCTTTCTGTGGACCCATCTCGGGCCCGCGCCCGCGCGC
GGCTGA

Upstream 100 bases:

>100_bases
GCCGCAAGGTCGAGGCCGCCGAGCGGCTCGGCATCGGCCGCAACACGATCACGCGCAAGATCCAGGAACTGCATCTGGAG
CCCTGAGCCTGCATCGCGGC

Downstream 100 bases:

>100_bases
GCGCACGCGGGGCAGGAGCAGGGCAGAAGCGGCAAGGCGGCGGCGTCAGCCGACGACCGCGACGACCGGCGTATGGTCGG
ACGGCTGCTCCCACGTGCGC

Product: peptidase S9 prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MRGAAHAAIGHNRALCCARDSVMSDSFRWPAAADPFRFLEALDSARARAWVDEQNARTRAALRDDDAYRTLAARLARAYL
PRERPVIPTRWRDWAYDLWQDDLHPKGLWRRARWDDWRAGRPEWKTLLDVDALGAAEGESWVFEQDAILYPHGDRALLSL
SPGGADAVVVREFDLVLRRFVDDGFTIDVPGHHTVGWIDRDTVYVSWDRGDAHSTAAGYPYDVRRWVRGTPLAQAPVVFR
GEPDDISAGAWFDPIDGRHVAWRSVDFFDAHTYRLTEAGEWARYDVPTHVEVGFWEGWLVLEPRLDWDCDGVRHAGGSLL
AIRERAFLDGSRAFTTLFEPQPSTSACTWTHTRHTLIASWLDDVHNRTLLWEPRETADGTWTWDARPLDWPGDGQIDVEP
VEPTLNDEVYVDVDTYLDPPECWLADLADRAADAASRRVLLDRPPVQFDAAGLVVRRASARSRDGTRVPYTLIGPRDALD
GATRVARPCLLSGYGGFALPNLPGYSDALGIGWLERGGVAAFAHIRGGGEFGPRWHVDAQREHRQRSFDDFIAVAEDLIA
TGVTTAAQLGIEGGSNGGLLVAACMVQRPALFGAVLCRVPLLDMRRYPKLHAGAAWLDEYGDPDDPDEGAALAAYSPYHR
VRDGVAYPPLLLTTSTRDDRVHPAHARKMAARMQALGHRNVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAR
G

Sequences:

>Translated_721_residues
MRGAAHAAIGHNRALCCARDSVMSDSFRWPAAADPFRFLEALDSARARAWVDEQNARTRAALRDDDAYRTLAARLARAYL
PRERPVIPTRWRDWAYDLWQDDLHPKGLWRRARWDDWRAGRPEWKTLLDVDALGAAEGESWVFEQDAILYPHGDRALLSL
SPGGADAVVVREFDLVLRRFVDDGFTIDVPGHHTVGWIDRDTVYVSWDRGDAHSTAAGYPYDVRRWVRGTPLAQAPVVFR
GEPDDISAGAWFDPIDGRHVAWRSVDFFDAHTYRLTEAGEWARYDVPTHVEVGFWEGWLVLEPRLDWDCDGVRHAGGSLL
AIRERAFLDGSRAFTTLFEPQPSTSACTWTHTRHTLIASWLDDVHNRTLLWEPRETADGTWTWDARPLDWPGDGQIDVEP
VEPTLNDEVYVDVDTYLDPPECWLADLADRAADAASRRVLLDRPPVQFDAAGLVVRRASARSRDGTRVPYTLIGPRDALD
GATRVARPCLLSGYGGFALPNLPGYSDALGIGWLERGGVAAFAHIRGGGEFGPRWHVDAQREHRQRSFDDFIAVAEDLIA
TGVTTAAQLGIEGGSNGGLLVAACMVQRPALFGAVLCRVPLLDMRRYPKLHAGAAWLDEYGDPDDPDEGAALAAYSPYHR
VRDGVAYPPLLLTTSTRDDRVHPAHARKMAARMQALGHRNVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAR
G
>Mature_721_residues
MRGAAHAAIGHNRALCCARDSVMSDSFRWPAAADPFRFLEALDSARARAWVDEQNARTRAALRDDDAYRTLAARLARAYL
PRERPVIPTRWRDWAYDLWQDDLHPKGLWRRARWDDWRAGRPEWKTLLDVDALGAAEGESWVFEQDAILYPHGDRALLSL
SPGGADAVVVREFDLVLRRFVDDGFTIDVPGHHTVGWIDRDTVYVSWDRGDAHSTAAGYPYDVRRWVRGTPLAQAPVVFR
GEPDDISAGAWFDPIDGRHVAWRSVDFFDAHTYRLTEAGEWARYDVPTHVEVGFWEGWLVLEPRLDWDCDGVRHAGGSLL
AIRERAFLDGSRAFTTLFEPQPSTSACTWTHTRHTLIASWLDDVHNRTLLWEPRETADGTWTWDARPLDWPGDGQIDVEP
VEPTLNDEVYVDVDTYLDPPECWLADLADRAADAASRRVLLDRPPVQFDAAGLVVRRASARSRDGTRVPYTLIGPRDALD
GATRVARPCLLSGYGGFALPNLPGYSDALGIGWLERGGVAAFAHIRGGGEFGPRWHVDAQREHRQRSFDDFIAVAEDLIA
TGVTTAAQLGIEGGSNGGLLVAACMVQRPALFGAVLCRVPLLDMRRYPKLHAGAAWLDEYGDPDDPDEGAALAAYSPYHR
VRDGVAYPPLLLTTSTRDDRVHPAHARKMAARMQALGHRNVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAR
G

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=315, Percent_Identity=35.2380952380952, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI284172438, Length=211, Percent_Identity=27.4881516587678, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI284172431, Length=211, Percent_Identity=27.4881516587678, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI284172420, Length=211, Percent_Identity=27.4881516587678, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI284172413, Length=211, Percent_Identity=27.4881516587678, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI70778815, Length=211, Percent_Identity=27.4881516587678, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI108860686, Length=211, Percent_Identity=27.4881516587678, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI108860692, Length=208, Percent_Identity=27.4038461538462, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788150, Length=290, Percent_Identity=29.6551724137931, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24583414, Length=274, Percent_Identity=36.8613138686131, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI221510989, Length=316, Percent_Identity=34.8101265822785, Blast_Score=171, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 80447; Mature: 80447

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGAAHAAIGHNRALCCARDSVMSDSFRWPAAADPFRFLEALDSARARAWVDEQNARTRA
CCCCCCCCCCCCCEEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
ALRDDDAYRTLAARLARAYLPRERPVIPTRWRDWAYDLWQDDLHPKGLWRRARWDDWRAG
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCC
RPEWKTLLDVDALGAAEGESWVFEQDAILYPHGDRALLSLSPGGADAVVVREFDLVLRRF
CCCHHHEEEHHHCCCCCCCCEEEECCCEEEECCCCEEEEECCCCCCEEEEHHHHHHHHHH
VDDGFTIDVPGHHTVGWIDRDTVYVSWDRGDAHSTAAGYPYDVRRWVRGTPLAQAPVVFR
HCCCCEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEE
GEPDDISAGAWFDPIDGRHVAWRSVDFFDAHTYRLTEAGEWARYDVPTHVEVGFWEGWLV
CCCCCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCEEECCCCEEEEEEECCEEE
LEPRLDWDCDGVRHAGGSLLAIRERAFLDGSRAFTTLFEPQPSTSACTWTHTRHTLIASW
ECCCCCCCCCCHHHCCCCEEEEEHHHHCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHH
LDDVHNRTLLWEPRETADGTWTWDARPLDWPGDGQIDVEPVEPTLNDEVYVDVDTYLDPP
HHHHCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCH
ECWLADLADRAADAASRRVLLDRPPVQFDAAGLVVRRASARSRDGTRVPYTLIGPRDALD
HHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCHHHH
GATRVARPCLLSGYGGFALPNLPGYSDALGIGWLERGGVAAFAHIRGGGEFGPRWHVDAQ
HHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCH
REHRQRSFDDFIAVAEDLIATGVTTAAQLGIEGGSNGGLLVAACMVQRPALFGAVLCRVP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEHHHHHHCHHHHHHHHHHCC
LLDMRRYPKLHAGAAWLDEYGDPDDPDEGAALAAYSPYHRVRDGVAYPPLLLTTSTRDDR
HHHHHCCCCHHCCHHHHHHCCCCCCCCCCCEEEECCCHHHHHCCCCCCCEEEEECCCCCC
VHPAHARKMAARMQALGHRNVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAR
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCC
G
C
>Mature Secondary Structure
MRGAAHAAIGHNRALCCARDSVMSDSFRWPAAADPFRFLEALDSARARAWVDEQNARTRA
CCCCCCCCCCCCCEEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
ALRDDDAYRTLAARLARAYLPRERPVIPTRWRDWAYDLWQDDLHPKGLWRRARWDDWRAG
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCC
RPEWKTLLDVDALGAAEGESWVFEQDAILYPHGDRALLSLSPGGADAVVVREFDLVLRRF
CCCHHHEEEHHHCCCCCCCCEEEECCCEEEECCCCEEEEECCCCCCEEEEHHHHHHHHHH
VDDGFTIDVPGHHTVGWIDRDTVYVSWDRGDAHSTAAGYPYDVRRWVRGTPLAQAPVVFR
HCCCCEEECCCCCEEEEEECCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEE
GEPDDISAGAWFDPIDGRHVAWRSVDFFDAHTYRLTEAGEWARYDVPTHVEVGFWEGWLV
CCCCCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCEEECCCCEEEEEEECCEEE
LEPRLDWDCDGVRHAGGSLLAIRERAFLDGSRAFTTLFEPQPSTSACTWTHTRHTLIASW
ECCCCCCCCCCHHHCCCCEEEEEHHHHCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHH
LDDVHNRTLLWEPRETADGTWTWDARPLDWPGDGQIDVEPVEPTLNDEVYVDVDTYLDPP
HHHHCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCH
ECWLADLADRAADAASRRVLLDRPPVQFDAAGLVVRRASARSRDGTRVPYTLIGPRDALD
HHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCHHHH
GATRVARPCLLSGYGGFALPNLPGYSDALGIGWLERGGVAAFAHIRGGGEFGPRWHVDAQ
HHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCH
REHRQRSFDDFIAVAEDLIATGVTTAAQLGIEGGSNGGLLVAACMVQRPALFGAVLCRVP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEHHHHHHCHHHHHHHHHHCC
LLDMRRYPKLHAGAAWLDEYGDPDDPDEGAALAAYSPYHRVRDGVAYPPLLLTTSTRDDR
HHHHHCCCCHHCCHHHHHHCCCCCCCCCCCEEEECCCHHHHHCCCCCCCEEEEECCCCCC
VHPAHARKMAARMQALGHRNVWYWENTDGGHGSADDLERAESDAAEFGFLWTHLGPAPAR
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]