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The map label for this gene is ntrB [H]

Identifier: 161524298

GI number: 161524298

Start: 1232544

End: 1233725

Strand: Direct

Name: ntrB [H]

Synonym: Bmul_1123

Alternate gene names: 161524298

Gene position: 1232544-1233725 (Clockwise)

Preceding gene: 161524297

Following gene: 161524299

Centisome position: 35.74

GC content: 66.58

Gene sequence:

>1182_bases
ATGAATCTTTGGCGCGGCAACGCGCGACACGCACGCACGATGGTTCTGAAGAATCTGATCAAGGCGAAGACGGGGCAGCC
CGAGCAATTGACCGACGACGAGCGGCTCGCCCGTTCCGGCCTGCTCGCCGGGCTCGAGGCGCTGCCGACGGTCGTGCTCG
TGCTCGACCGCAAGACGCTCAGGATCGCGTTCGCGAATCCGTCCGCGGAGGCGATGCTCGACATCTCGCGCCGCCAGCTC
GCGCAGCGGCCGTGGGGCGAGATTTTCCCGAACGCGAACGAGCTCGCCTCGACGATCACCGCGATCGGCGAGGAGCGCTT
TCACGCGACGCATCTGGACACCGTGCTCGACCGGCCCGGCCACGAGCTGCTGCACGTGCATGCGATCGTCGGCTTTCTCG
AGAGCGCGCCCGATTTCGTGCTCGTCGAGCTGTTCGAGAACGAGCGGCAGTCGCGCACCGATCGCGAGGAGCGCATTCAC
GACCTGACCGCGGTCAACAAGCAGCTGATCCGCAATCTCGCGCACGAGATCAAGAACCCGCTCGGCGGCATCCGCGGGGC
CGCGCAACTGCTCGAATTCGAGCTCGGCGAGCGCGAACGCGGCGAGCTGCGCGAATACACGCAGGTGATCATCAAGGAGT
CCGATCGCCTGCAGACGCTCGTCGACCGGCTGCTGGAGCCGCACCGGCATCCGCACATCGTCGGCGACGTGAACATCCAC
GAAGTATGCGAACGCGTGCGCGCCGTGATGCTCGCCGAATTCCCGCGCGGGCTCACGATCGTGCGCGACTACGACGTCAG
CGTGCCGGACCTGCGCGGCGACAAGGAGCAGCTGATCCAGGCGCTGCTGAACATCGTGCGCAACGCGGCGCAGGCATTGC
GCGAACGGATCGCGCAGGGCGACGCGAAGATCGAACTGCGCACGCGCGTCGCGCGCAAGGTCACGATCGCGAAGCGGCTG
TACCGGCTGGCACTGGACTTGCATGTGATCGACAACGGCCCCGGCATTCCGGACGAGATCCGCGACCGGATCTTCTATCC
GCTCGTCTCCGGCCGCGACGACGGCAGCGGCCTCGGCCTGACGCTCGCCCAGACGTTCGTGCAGCAGCACGACGGGACGA
TCGAGGTCGAAAGCCGGCCGGGACGTACCGAATTTCAGATTCTGTTGCCGCTCGACGCATGA

Upstream 100 bases:

>100_bases
GCCGTCATGCCGATGCGTTCCCGTCGCGCGGTTCGCGGCGGGGACGGCGGAGTCTGAAAAGGGGACGGCGCCCAGCCGTC
CCTTTTTTGTTCGCGACTGG

Downstream 100 bases:

>100_bases
GCGGCGACATTGCGATACACCAATATCTGACCGACCTATGAAGCCGATCTGGATAGTAGACGACGATCAATCGATCCGCT
GGGTGCTCGAGAAGGCGCTC

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNLWRGNARHARTMVLKNLIKAKTGQPEQLTDDERLARSGLLAGLEALPTVVLVLDRKTLRIAFANPSAEAMLDISRRQL
AQRPWGEIFPNANELASTITAIGEERFHATHLDTVLDRPGHELLHVHAIVGFLESAPDFVLVELFENERQSRTDREERIH
DLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIH
EVCERVRAVMLAEFPRGLTIVRDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRVARKVTIAKRL
YRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGTIEVESRPGRTEFQILLPLDA

Sequences:

>Translated_393_residues
MNLWRGNARHARTMVLKNLIKAKTGQPEQLTDDERLARSGLLAGLEALPTVVLVLDRKTLRIAFANPSAEAMLDISRRQL
AQRPWGEIFPNANELASTITAIGEERFHATHLDTVLDRPGHELLHVHAIVGFLESAPDFVLVELFENERQSRTDREERIH
DLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIH
EVCERVRAVMLAEFPRGLTIVRDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRVARKVTIAKRL
YRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGTIEVESRPGRTEFQILLPLDA
>Mature_393_residues
MNLWRGNARHARTMVLKNLIKAKTGQPEQLTDDERLARSGLLAGLEALPTVVLVLDRKTLRIAFANPSAEAMLDISRRQL
AQRPWGEIFPNANELASTITAIGEERFHATHLDTVLDRPGHELLHVHAIVGFLESAPDFVLVELFENERQSRTDREERIH
DLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIH
EVCERVRAVMLAEFPRGLTIVRDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRVARKVTIAKRL
YRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGTIEVESRPGRTEFQILLPLDA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=351, Percent_Identity=42.4501424501425, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=27.1708683473389, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790436, Length=265, Percent_Identity=28.6792452830189, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=27.1604938271605, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44427; Mature: 44427

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLWRGNARHARTMVLKNLIKAKTGQPEQLTDDERLARSGLLAGLEALPTVVLVLDRKTL
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEE
RIAFANPSAEAMLDISRRQLAQRPWGEIFPNANELASTITAIGEERFHATHLDTVLDRPG
EEEECCCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HELLHVHAIVGFLESAPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIKNP
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHH
EVCERVRAVMLAEFPRGLTIVRDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQG
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DAKIELRTRVARKVTIAKRLYRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGLGL
CCEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
TLAQTFVQQHDGTIEVESRPGRTEFQILLPLDA
HHHHHHHHHCCCEEEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNLWRGNARHARTMVLKNLIKAKTGQPEQLTDDERLARSGLLAGLEALPTVVLVLDRKTL
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEE
RIAFANPSAEAMLDISRRQLAQRPWGEIFPNANELASTITAIGEERFHATHLDTVLDRPG
EEEECCCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HELLHVHAIVGFLESAPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIKNP
HHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHH
EVCERVRAVMLAEFPRGLTIVRDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQG
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DAKIELRTRVARKVTIAKRLYRLALDLHVIDNGPGIPDEIRDRIFYPLVSGRDDGSGLGL
CCEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
TLAQTFVQQHDGTIEVESRPGRTEFQILLPLDA
HHHHHHHHHCCCEEEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]