Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 161524297

GI number: 161524297

Start: 1231004

End: 1232419

Strand: Direct

Name: glnA [H]

Synonym: Bmul_1122

Alternate gene names: 161524297

Gene position: 1231004-1232419 (Clockwise)

Preceding gene: 161524295

Following gene: 161524298

Centisome position: 35.7

GC content: 63.06

Gene sequence:

>1416_bases
ATGAGTAAAACCGTCGCCGACGTCATGCAGCTCGTGAAGGACGAGGACGTCAAGTTTGTCGATTTCCGCTTCACGGATAC
GCGCGGCAAGGAGCAGCACGTGTCGGTGCCGGTTTCGGCGTTTGACGAAGACAAGTTCGAAAGCGGCCATGCGTTCGACG
GCTCGTCGATCGCGGGCTGGAAGGGCATCGAGGCGTCGGACATGCTGCTCATGCCGGATCCGAGCGCTGCGTTCATCGAC
CCGTTCTACGAAGAGTCGACGCTCGTGCTGACCTGCGACGTGGTCGAGCCGGCGGACGGCAAGGGCTACGAGCGCGACCC
GCGTTCGCTCGCGAAGCGCGCGGAAGCGTACCTGAAGAGCACGGGCCTCGGCGACACCGCGTACTTCGGTCCGGAGCCGG
AATTCTTCATCTTCGACTCGGTCCAGTGGAACACGGACATGTCGGGCTGCTTCGTGAAGATCAACTCGGAAGAAGCGCCG
TGGTCGGCCGGCAAGGAATTCGAAGGCGGCAACACGGGCCACCGTCCGGGCACGAAGGGCGGCTACTTCCCGGTCGCGCC
GGTCGACACGTTCCAGGACATGCGTTCGGAAATGTGCCTGCTGCTCGAACAGCTCGGCATTCCGGTCGAAGTGCACCACC
ACGAAGTGGCAGGCCAGGGCCAGAACGAAATCGGCACGAAGTTCTCGACGCTCGTGCAGCGCGCCGACTGGACGCAATGG
TCGAAGTACATCATCCACAACGTCGCGCACTCGTACGGCAAGACGGCGACGTTCATGCCGAAGCCGGTCGTCGGCGACAA
CGGTTCGGGCATGCACGTTCACCAGTCGATCTGGAAGGACGGCCAGAACCTGTTCGCGGGCAACGGCTATGCCGGCCTGT
CGGAATTCGCGCTGTTCTACATCGGCGGGATCATCAAGCACGCCCGCGCGCTGAACGCGATCACGAACCCGACGACGAAC
TCGTACAAGCGTCTGGTGCCGCACTTCGAGGCGCCGGTCAAGCTCGCGTACTCGGCACGCAACCGTTCGGCATCGATCCG
CATTCCGCACGTGTCGAACCCGAAGGGCCGCCGTATCGAAACGCGCTTCCCGGATCCGATGGCGAACCCGTACCTGTGCT
TCTCGGCGCTGATGATGGCCGGTCTGGACGGCGTGCAGAACAAGATCCATCCGGGCGAAGCAGCGGACAAGAACCTGTAC
GACCTGCCGCCGGAAGAGGATGCAAAGATCCCGACCGTCTGCGCGGGCCTCGATCAGGCGCTCGAAGCGCTCGACAAGGA
TCGCGAGTTCCTGACGCGCGGCGGCGTGTTCACGGACGGCATGATCGACGCGTACCTCGCGCTGAAGGAGCAGGAGCTGG
CGAAGTTCCGCATGACGACGCATCCGATCGAGTTCGAGATGTACTACTCGCTGTAA

Upstream 100 bases:

>100_bases
AAGAATTCGGTCGAATCGGGGCGCTGCGCACGGGCCCGATCAGCTCGATAAACGAGGCGGTTCCCAGTCCGCCGGAATTG
TTCAATCAGGAGAAGAGGTT

Downstream 100 bases:

>100_bases
TCGGCGATGGCGCTTCGATTCAGCGCCGTCATGCCGATGCGTTCCCGTCGCGCGGTTCGCGGCGGGGACGGCGGAGTCTG
AAAAGGGGACGGCGCCCAGC

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGWKGIEASDMLLMPDPSAAFID
PFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGLGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAP
WSAGKEFEGGNTGHRPGTKGGYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQW
SKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALFYIGGIIKHARALNAITNPTTN
SYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLY
DLPPEEDAKIPTVCAGLDQALEALDKDREFLTRGGVFTDGMIDAYLALKEQELAKFRMTTHPIEFEMYYSL

Sequences:

>Translated_471_residues
MSKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGWKGIEASDMLLMPDPSAAFID
PFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGLGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAP
WSAGKEFEGGNTGHRPGTKGGYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQW
SKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALFYIGGIIKHARALNAITNPTTN
SYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLY
DLPPEEDAKIPTVCAGLDQALEALDKDREFLTRGGVFTDGMIDAYLALKEQELAKFRMTTHPIEFEMYYSL
>Mature_470_residues
SKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGWKGIEASDMLLMPDPSAAFIDP
FYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKSTGLGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAPW
SAGKEFEGGNTGHRPGTKGGYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQWS
KYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALFYIGGIIKHARALNAITNPTTNS
YKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIETRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLYD
LPPEEDAKIPTVCAGLDQALEALDKDREFLTRGGVFTDGMIDAYLALKEQELAKFRMTTHPIEFEMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=65.0862068965517, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=402, Percent_Identity=26.3681592039801, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52240; Mature: 52109

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGW
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDPSAAFIDPFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKS
CCCCCCCEEEECCCCCHHCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGLGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAPWSAGKEFEGGNTGHRPGTKG
CCCCCCCCCCCCCCEEEEECEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQW
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHCCHHHH
SKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALFY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPTTNSYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIE
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEE
TRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLYDLPPEEDAKIPTVCAGLDQA
CCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALDKDREFLTRGGVFTDGMIDAYLALKEQELAKFRMTTHPIEFEMYYSL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
SKTVADVMQLVKDEDVKFVDFRFTDTRGKEQHVSVPVSAFDEDKFESGHAFDGSSIAGW
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDPSAAFIDPFYEESTLVLTCDVVEPADGKGYERDPRSLAKRAEAYLKS
CCCCCCCEEEECCCCCHHCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
TGLGDTAYFGPEPEFFIFDSVQWNTDMSGCFVKINSEEAPWSAGKEFEGGNTGHRPGTKG
CCCCCCCCCCCCCCEEEEECEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPVDTFQDMRSEMCLLLEQLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQW
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCCHHHHHHHHHHHHHCCHHHH
SKYIIHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSEFALFY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPTTNSYKRLVPHFEAPVKLAYSARNRSASIRIPHVSNPKGRRIE
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCEEE
TRFPDPMANPYLCFSALMMAGLDGVQNKIHPGEAADKNLYDLPPEEDAKIPTVCAGLDQA
CCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LEALDKDREFLTRGGVFTDGMIDAYLALKEQELAKFRMTTHPIEFEMYYSL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]