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The map label for this gene is hutC [H]

Identifier: 161524274

GI number: 161524274

Start: 1202544

End: 1203239

Strand: Direct

Name: hutC [H]

Synonym: Bmul_1099

Alternate gene names: 161524274

Gene position: 1202544-1203239 (Clockwise)

Preceding gene: 161524273

Following gene: 161524275

Centisome position: 34.87

GC content: 67.24

Gene sequence:

>696_bases
ATGAGCGCGCCGGTCTACCAGGAGATCAAGGATTTCATCCTGGCCCGCATCCACGCCGGCGAATGGGCGGAGGGCGATCA
GGTGCCGTCCGAGAACGAACTGGCCCGCGAGTTCAAGGTCGCGCGCATGACCGTCAACCGCGCGCTGCGCGAGTTGACGG
ACGAGCAGGTGCTCACGCGGATGAAGGGCGCCGGCACCTACGTCGCGCGGCCGAAGTACGAGTCGACGCTGGTCGCGATC
CGCAGCATCTCCGAGGAGGTCGACGCGCGCGGACATGCGTATCACGCGCGCGTGCTCGGCCTCGAGACGATCCGCGCCGA
CGACACGCTCGCCGACGAGATGCAGGTGGCCGTGCGCGCCAAGCTGTTTCATTCGCAGGTGCTGCACTTCGAGAACGACG
AACCGGTGCAGCTCGAAGAGCGCTGGGTGAACCCGGCGGTCGCGCCGGATTACGCGGCGCAGGACTTCACGAACACGACG
CCGAACCAGTACCTGATGCGCGCGGCGCCGCTGCAGCGCGTCGAGTACCGGATCGAAGCGGCGGTGCCCGCGCCCGAGCG
GCGCGAGCAGCTTCGGATGGACGACGTCGAGCCGTGTCTGGTTTTACATCGACGCACCTGGTCGCAGGGCGTCGTCGCCT
CGGTGGCGAATTTGTGGCATCCCGGCAGCCGTTATCGCTTCACCGGGCATTTCTGA

Upstream 100 bases:

>100_bases
CACTACTTTGCGCCGGACATCGCCGTGATCGCGAAGCTCGTCGTCGAGCGCGCGTTCGCGAAGCACTGCCCGTTCGCCTT
CGCATCGGAGCAGTAAGCCG

Downstream 100 bases:

>100_bases
TTCCTATTGATATTTCCTTTCCTTCGGGAGCAGTCGTCATGAACCATCCGAAACACATCGATCCCCGTCTCGATCCGACG
CGCACGATCCGCGCCCCGCG

Product: histidine utilization repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSAPVYQEIKDFILARIHAGEWAEGDQVPSENELAREFKVARMTVNRALRELTDEQVLTRMKGAGTYVARPKYESTLVAI
RSISEEVDARGHAYHARVLGLETIRADDTLADEMQVAVRAKLFHSQVLHFENDEPVQLEERWVNPAVAPDYAAQDFTNTT
PNQYLMRAAPLQRVEYRIEAAVPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF

Sequences:

>Translated_231_residues
MSAPVYQEIKDFILARIHAGEWAEGDQVPSENELAREFKVARMTVNRALRELTDEQVLTRMKGAGTYVARPKYESTLVAI
RSISEEVDARGHAYHARVLGLETIRADDTLADEMQVAVRAKLFHSQVLHFENDEPVQLEERWVNPAVAPDYAAQDFTNTT
PNQYLMRAAPLQRVEYRIEAAVPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF
>Mature_230_residues
SAPVYQEIKDFILARIHAGEWAEGDQVPSENELAREFKVARMTVNRALRELTDEQVLTRMKGAGTYVARPKYESTLVAIR
SISEEVDARGHAYHARVLGLETIRADDTLADEMQVAVRAKLFHSQVLHFENDEPVQLEERWVNPAVAPDYAAQDFTNTTP
NQYLMRAAPLQRVEYRIEAAVPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF

Specific function: Repressor which binds to the hutP region in the histidine utilization (hut) operon. It blocks the expression of all the hut genes in the absence of inducer [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786950, Length=199, Percent_Identity=31.1557788944724, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87082252, Length=147, Percent_Identity=25.8503401360544, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788418, Length=211, Percent_Identity=24.1706161137441, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790118, Length=139, Percent_Identity=23.7410071942446, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010248
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 26425; Mature: 26294

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVYQEIKDFILARIHAGEWAEGDQVPSENELAREFKVARMTVNRALRELTDEQVLTR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGAGTYVARPKYESTLVAIRSISEEVDARGHAYHARVLGLETIRADDTLADEMQVAVRA
HHCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KLFHSQVLHFENDEPVQLEERWVNPAVAPDYAAQDFTNTTPNQYLMRAAPLQRVEYRIEA
HHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
AVPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF
CCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure 
SAPVYQEIKDFILARIHAGEWAEGDQVPSENELAREFKVARMTVNRALRELTDEQVLTR
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGAGTYVARPKYESTLVAIRSISEEVDARGHAYHARVLGLETIRADDTLADEMQVAVRA
HHCCCCEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
KLFHSQVLHFENDEPVQLEERWVNPAVAPDYAAQDFTNTTPNQYLMRAAPLQRVEYRIEA
HHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
AVPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF
CCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2203754; 2834335 [H]