Click here to switch to the map view.

The map label for this gene is purK [H]

Identifier: 161523855

GI number: 161523855

Start: 738345

End: 739541

Strand: Direct

Name: purK [H]

Synonym: Bmul_0675

Alternate gene names: 161523855

Gene position: 738345-739541 (Clockwise)

Preceding gene: 161523854

Following gene: 161523856

Centisome position: 21.41

GC content: 71.09

Gene sequence:

>1197_bases
ATGACCGCAACTCCTGATTCCGTTTCCCCGATCCTGCCCGGCGCGTGGCTGGGCATGGTCGGCGGCGGCCAGCTCGGCCG
CATGTTCTGTTTTGCCGCCCAGTCGATGGGCTATCGCGTCGCCGTGCTCGATCCCGATCCGGCGAGCCCCGCCGGCGCGG
TCGCCGATCGCCACCTGCGCGCCGCGTATGACGACGAAGCCGCGCTGACCGAACTGGCCGCGCTGTGCGACGCGGTATCG
ACCGAATTCGAGAACGTGCCGGCCGCGAGCCTCGATTTCCTCGCGCGCACGACGTTCGTCGCGCCGGCCGGCCGCTGCGT
CGCGATCGCGCAGGACCGGATCGCCGAAAAGCGCTTCATCGAGGCGTCGGGCGTACCGGTCGCGCCGCACGTCGCGATCG
AATCGGCCGACGCGCTCGCGGCGCTCGACGACGCAGCGCTCGACGCGGTGCTGCCGGGCATTCTGAAGACCGCGCGGCTC
GGCTACGACGGCAAGGGCCAGGTGCGTGTGCGCACCGCGCAGGAAGCGCGCGAGGCGTATGCGTCGCTCGGCGGCGTGCC
GTGCGTGCTCGAAAAGCGCCTGCCGCTGAAATACGAGGTGTCCGCACTGATCGCGCGCGGCGCCGACGGCCGCTCGGCCG
CGTTCCCGCTCGCGCAGAACGCGCACCACAACGGCATTCTCGCGCTGACCGTCGTGCCGGCGCCGGCCGCCGACGACGCG
CTCGTCGCGCAGGCGCAGCAGGCCGCCGTGCGCATCGCCGATACGCTCGGCTACGTCGGCGTGCTGTGCGTCGAGTTCTT
CGTGCTCGAAGACGGTTCGCTCGTGGCGAACGAGATGGCGCCGCGCCCGCACAATTCCGGCCACTACACGGTCGATGCGT
GCGCAACGAGCCAGTTCGAGCAGCAGGTGCGCGCAATGACGCGCATGCCGCTCGGCAATCCGCGCCAGCATTCGCCGGCC
GCGATGCTGAACATTCTCGGCGACGTCTGGTTTCCGCAAGGCGCGGAAAGCGACGCCGTCACGCCGCCGTGGGACGCGGT
TGCCGCGATGCCGACCGCGCATCTGCATCTGTACGGCAAGGAAGAGGCGCGCGTCGGCCGCAAGATGGGCCACGTGAATT
TCACGGCCGCGACGCGCGACGAAGCGGTCGCCGCCGCGCACGCGTGCGCGCAGCTGCTGCGCGTGCCGCTCGACTGA

Upstream 100 bases:

>100_bases
CCGCCGCTCGAATGACGGGACGGCGCGCGCCGCACGGCACGCGCCGGATTTTCCCAGTGCGGCGGGTGCTTCCCGTTTCC
CGTCGCGATCGATCACTGAC

Downstream 100 bases:

>100_bases
GGCGCCGGCCGATGTCCACCGATCTCCCGAAGTCCGTGACGCCCGCGCAGATCGACGCGGCGGCCGCGCTGCTCGATGCG
GGGCAGCTCGTCGCGTTTCC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLRAAYDDEAALTELAALCDAVS
TEFENVPAASLDFLARTTFVAPAGRCVAIAQDRIAEKRFIEASGVPVAPHVAIESADALAALDDAALDAVLPGILKTARL
GYDGKGQVRVRTAQEAREAYASLGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNAHHNGILALTVVPAPAADDA
LVAQAQQAAVRIADTLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACATSQFEQQVRAMTRMPLGNPRQHSPA
AMLNILGDVWFPQGAESDAVTPPWDAVAAMPTAHLHLYGKEEARVGRKMGHVNFTAATRDEAVAAAHACAQLLRVPLD

Sequences:

>Translated_398_residues
MTATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLRAAYDDEAALTELAALCDAVS
TEFENVPAASLDFLARTTFVAPAGRCVAIAQDRIAEKRFIEASGVPVAPHVAIESADALAALDDAALDAVLPGILKTARL
GYDGKGQVRVRTAQEAREAYASLGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNAHHNGILALTVVPAPAADDA
LVAQAQQAAVRIADTLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACATSQFEQQVRAMTRMPLGNPRQHSPA
AMLNILGDVWFPQGAESDAVTPPWDAVAAMPTAHLHLYGKEEARVGRKMGHVNFTAATRDEAVAAAHACAQLLRVPLD
>Mature_397_residues
TATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLRAAYDDEAALTELAALCDAVST
EFENVPAASLDFLARTTFVAPAGRCVAIAQDRIAEKRFIEASGVPVAPHVAIESADALAALDDAALDAVLPGILKTARLG
YDGKGQVRVRTAQEAREAYASLGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNAHHNGILALTVVPAPAADDAL
VAQAQQAAVRIADTLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACATSQFEQQVRAMTRMPLGNPRQHSPAA
MLNILGDVWFPQGAESDAVTPPWDAVAAMPTAHLHLYGKEEARVGRKMGHVNFTAATRDEAVAAAHACAQLLRVPLD

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=379, Percent_Identity=34.8284960422164, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1788155, Length=385, Percent_Identity=27.7922077922078, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324702, Length=377, Percent_Identity=32.0954907161804, Blast_Score=200, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41725; Mature: 41594

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLR
CCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
AAYDDEAALTELAALCDAVSTEFENVPAASLDFLARTTFVAPAGRCVAIAQDRIAEKRFI
HHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
EASGVPVAPHVAIESADALAALDDAALDAVLPGILKTARLGYDGKGQVRVRTAQEAREAY
HCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHH
ASLGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNAHHNGILALTVVPAPAADDA
HHHCCCCEEECCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHH
LVAQAQQAAVRIADTLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACATSQFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEEHHHHHHHH
QQVRAMTRMPLGNPRQHSPAAMLNILGDVWFPQGAESDAVTPPWDAVAAMPTAHLHLYGK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCEEEEEECC
EEARVGRKMGHVNFTAATRDEAVAAAHACAQLLRVPLD
HHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLR
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
AAYDDEAALTELAALCDAVSTEFENVPAASLDFLARTTFVAPAGRCVAIAQDRIAEKRFI
HHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHH
EASGVPVAPHVAIESADALAALDDAALDAVLPGILKTARLGYDGKGQVRVRTAQEAREAY
HCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHH
ASLGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNAHHNGILALTVVPAPAADDA
HHHCCCCEEECCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHH
LVAQAQQAAVRIADTLGYVGVLCVEFFVLEDGSLVANEMAPRPHNSGHYTVDACATSQFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCCCCCEEEEHHHHHHHH
QQVRAMTRMPLGNPRQHSPAAMLNILGDVWFPQGAESDAVTPPWDAVAAMPTAHLHLYGK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCEEEEEECC
EEARVGRKMGHVNFTAATRDEAVAAAHACAQLLRVPLD
HHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]