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The map label for this gene is kefB [H]

Identifier: 161523710

GI number: 161523710

Start: 577653

End: 579689

Strand: Direct

Name: kefB [H]

Synonym: Bmul_0530

Alternate gene names: 161523710

Gene position: 577653-579689 (Clockwise)

Preceding gene: 161523698

Following gene: 161523711

Centisome position: 16.75

GC content: 68.97

Gene sequence:

>2037_bases
GTGGCCCCGACGAACGAGGACGCTCCGCCCGTGATTTCCCCGCTCGAAATGACCCTGCTGCTGCTGCTGGCCTCGGTGGT
AGGCGTCGTCGTATTTCGCTCGCTGAATCTGCCGCCGATGCTCGGCTATCTGGCCGTCGGCATCCTGGTCGGCCCGCACG
CGTTCGGCGTGGCGGGCGACCTCGAGCGCGCCGAGCATCTCGCCGAGTTCGGCGTCGTGTTCCTGATGTTCTCGATCGGC
CTCGAGTTCTCGCTCGCGAAGCTGCGCGCGATGCAGCGGCTCGTGTTCGGCCTCGGGCTGATGCAGGTGGTCGCGACGCT
CGCGCTCGCGGTCGTGCTCGGCATGCTGTTCCGGCGCTGGGTCGACATCACGTGGCAGGGCAGCGTCGCGCTCGGCGGCG
CGCTTGCGATGTCGTCGACGGCGATCGTCTCGAAGATGCTCGCCGAGCGGCTCGAGATCGAGACCGAGCACGGCCGCAAC
ATCTTCGGCGTGCTGCTGTTCCAGGATCTCGCGGTGGTGCCGCTGCTGATCGTGATCGCTGCATTCGGCGCCGAGTCGTC
GAAGGATCTCGCGCTGACGCTCGGCTTCGCGGCCGTCAAGATCGTGATCGCGCTCTCGCTGCTGCTCGTCATCGGCCAGC
GCTTCATGACGCGCTGGCTCAACGTCGTCGCGCGGCGCCGCTCGCAGGAACTCTTCGTGCTGAACCTGCTGCTCGTCACG
CTCGGCGCCGCGTTCATCACCGACCGCTTCGGGCTGTCGCTCGCGCTCGGCGCATTCATCGCCGGCATGCTGATCGCCGA
GACGCCGTTCCGTCATCAGGTCGAGGAGGACATCAAGCCGTTTCGCGACGTGCTGCTCGGCCTGTTCTTCGTGACGACCG
GGATGCTGCTCGACCCGCGCGTGATCTGGGAGCATCCGCTGCTCGTCGGCGGCTTTTTCGTCGGCCAGATCCTGTTCAAG
GCGACGATGATCGCGGGCCTCGCGCGGCTGTTCGGCGCGACGCCCGGCGTCGCGATGCGCACCGGCATCGGGCTTGCGCA
GGCCGGCGAATTCGGCTTCGTGCTGCTGAACCTGATCCTCGATCGCCATCTCGTCGACGCGACGCTGCTGCAGGCGATTC
TTGCGTCGATGCTGCTGTCGATGCTCGCCGCGCCGTTCCTGATCCAGAACGCCGACCGCATCGTGATGCGGCTGTCGTCG
ACCGAATGGATGCAGCAGTCGCTGCAGATGACGCGCATCGCGACGCAGAGCCTCAAGCAGCGCGGCCACGTGATCATCTG
CGGCTACGGCCGCGCCGGCCAGAACCTCGCGCGGATGCTCGAACAGGAAGGCATCTCGTACGTCGCGCTCGACCTCGACC
CCGATCGCGTGAGCGCGGCCGCGACGGCCGGCGAATCGGTCGTGTTCGGCGACGCCGCGCGCCGCGAGTCGCTGCTCGCG
GCCGGTATCCATCGCGCGGCGGCCGTCGCCATCACGTACGCGAACACGCCGTCCGCGCTGCGCGTGCTGCACCACGTGCA
CGAACTCGAGCCGACGCTGCCGGCGATCGTGCGCACCGTCGACGACGCCGATCTCGAGAAGCTGCTCGCGGCCGGCGCGA
CCGAGGTGATTCCGGAGATCGTCGAAGGCAGCCTGATGCTCGCGTCGCATACGCTGGTGCTCGTCGGCGTGCCGATGCGC
AAGGTCGTGCGGCGCGTCGAGGAGATGCGCGACGAACGCTACAGCCTGCTGCGCGGCTATTTCCACGGCGCCGACGACGC
GGACGACGACGATCACGAGCAGGTACGGCTACAATCGGTGCCGGTCGACGCGCGCGCGGACGCGGTCGGGCGCTCGCTGG
AGGAGCTCGGGCTCTACGCGCTGGGGCTCGAAGTCACCGCGATCCGCCGGCACGGCATTCGCGGCGTCGAACCCGATCCG
CAGACGAAGCTGCGTGCGAGCGACATCGTCGTGCTGCGCGGGCTGCCCGAGGCGCTCGCGCTCGCGGAGGAGCGGCTCTC
GCGCCATCGCCGCGATCCGCCGGCCGTGGCCGCGTGA

Upstream 100 bases:

>100_bases
TCATGCGCGGAGTATAGCAAAGGCGTTTGTTCGCCGCGCCGGCCCATTCGGACGTGTCCGATGCCGCCGGTCCATCGCCA
CTTGCCGCGCCCGTCTTGCC

Downstream 100 bases:

>100_bases
GCCGCGATTCATTACCCGACCCGTATTTTTTCGAAACGGTGCCCGATCTTCGCGCACCGTTTTTTTCGGAGAGTTTCATG
TCGCATTCGTCGTCGGGCGC

Product: sodium/hydrogen exchanger

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MAPTNEDAPPVISPLEMTLLLLLASVVGVVVFRSLNLPPMLGYLAVGILVGPHAFGVAGDLERAEHLAEFGVVFLMFSIG
LEFSLAKLRAMQRLVFGLGLMQVVATLALAVVLGMLFRRWVDITWQGSVALGGALAMSSTAIVSKMLAERLEIETEHGRN
IFGVLLFQDLAVVPLLIVIAAFGAESSKDLALTLGFAAVKIVIALSLLLVIGQRFMTRWLNVVARRRSQELFVLNLLLVT
LGAAFITDRFGLSLALGAFIAGMLIAETPFRHQVEEDIKPFRDVLLGLFFVTTGMLLDPRVIWEHPLLVGGFFVGQILFK
ATMIAGLARLFGATPGVAMRTGIGLAQAGEFGFVLLNLILDRHLVDATLLQAILASMLLSMLAAPFLIQNADRIVMRLSS
TEWMQQSLQMTRIATQSLKQRGHVIICGYGRAGQNLARMLEQEGISYVALDLDPDRVSAAATAGESVVFGDAARRESLLA
AGIHRAAAVAITYANTPSALRVLHHVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVLVGVPMR
KVVRRVEEMRDERYSLLRGYFHGADDADDDDHEQVRLQSVPVDARADAVGRSLEELGLYALGLEVTAIRRHGIRGVEPDP
QTKLRASDIVVLRGLPEALALAEERLSRHRRDPPAVAA

Sequences:

>Translated_678_residues
MAPTNEDAPPVISPLEMTLLLLLASVVGVVVFRSLNLPPMLGYLAVGILVGPHAFGVAGDLERAEHLAEFGVVFLMFSIG
LEFSLAKLRAMQRLVFGLGLMQVVATLALAVVLGMLFRRWVDITWQGSVALGGALAMSSTAIVSKMLAERLEIETEHGRN
IFGVLLFQDLAVVPLLIVIAAFGAESSKDLALTLGFAAVKIVIALSLLLVIGQRFMTRWLNVVARRRSQELFVLNLLLVT
LGAAFITDRFGLSLALGAFIAGMLIAETPFRHQVEEDIKPFRDVLLGLFFVTTGMLLDPRVIWEHPLLVGGFFVGQILFK
ATMIAGLARLFGATPGVAMRTGIGLAQAGEFGFVLLNLILDRHLVDATLLQAILASMLLSMLAAPFLIQNADRIVMRLSS
TEWMQQSLQMTRIATQSLKQRGHVIICGYGRAGQNLARMLEQEGISYVALDLDPDRVSAAATAGESVVFGDAARRESLLA
AGIHRAAAVAITYANTPSALRVLHHVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVLVGVPMR
KVVRRVEEMRDERYSLLRGYFHGADDADDDDHEQVRLQSVPVDARADAVGRSLEELGLYALGLEVTAIRRHGIRGVEPDP
QTKLRASDIVVLRGLPEALALAEERLSRHRRDPPAVAA
>Mature_677_residues
APTNEDAPPVISPLEMTLLLLLASVVGVVVFRSLNLPPMLGYLAVGILVGPHAFGVAGDLERAEHLAEFGVVFLMFSIGL
EFSLAKLRAMQRLVFGLGLMQVVATLALAVVLGMLFRRWVDITWQGSVALGGALAMSSTAIVSKMLAERLEIETEHGRNI
FGVLLFQDLAVVPLLIVIAAFGAESSKDLALTLGFAAVKIVIALSLLLVIGQRFMTRWLNVVARRRSQELFVLNLLLVTL
GAAFITDRFGLSLALGAFIAGMLIAETPFRHQVEEDIKPFRDVLLGLFFVTTGMLLDPRVIWEHPLLVGGFFVGQILFKA
TMIAGLARLFGATPGVAMRTGIGLAQAGEFGFVLLNLILDRHLVDATLLQAILASMLLSMLAAPFLIQNADRIVMRLSST
EWMQQSLQMTRIATQSLKQRGHVIICGYGRAGQNLARMLEQEGISYVALDLDPDRVSAAATAGESVVFGDAARRESLLAA
GIHRAAAVAITYANTPSALRVLHHVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVLVGVPMRK
VVRRVEEMRDERYSLLRGYFHGADDADDDDHEQVRLQSVPVDARADAVGRSLEELGLYALGLEVTAIRRHGIRGVEPDPQ
TKLRASDIVVLRGLPEALALAEERLSRHRRDPPAVAA

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=343, Percent_Identity=27.4052478134111, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1789749, Length=543, Percent_Identity=33.3333333333333, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1786232, Length=609, Percent_Identity=30.5418719211823, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1786685, Length=535, Percent_Identity=29.3457943925234, Blast_Score=194, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 73467; Mature: 73335

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPTNEDAPPVISPLEMTLLLLLASVVGVVVFRSLNLPPMLGYLAVGILVGPHAFGVAGD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCCCC
LERAEHLAEFGVVFLMFSIGLEFSLAKLRAMQRLVFGLGLMQVVATLALAVVLGMLFRRW
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDITWQGSVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLLIVIA
HCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHH
AFGAESSKDLALTLGFAAVKIVIALSLLLVIGQRFMTRWLNVVARRRSQELFVLNLLLVT
HHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LGAAFITDRFGLSLALGAFIAGMLIAETPFRHQVEEDIKPFRDVLLGLFFVTTGMLLDPR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VIWEHPLLVGGFFVGQILFKATMIAGLARLFGATPGVAMRTGIGLAQAGEFGFVLLNLIL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHCCCCHHHHHHHHHH
DRHLVDATLLQAILASMLLSMLAAPFLIQNADRIVMRLSSTEWMQQSLQMTRIATQSLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RGHVIICGYGRAGQNLARMLEQEGISYVALDLDPDRVSAAATAGESVVFGDAARRESLLA
CCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEECCHHHHHHHHH
AGIHRAAAVAITYANTPSALRVLHHVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEI
HHHHHHHEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCHHHHHHHH
VEGSLMLASHTLVLVGVPMRKVVRRVEEMRDERYSLLRGYFHGADDADDDDHEQVRLQSV
HCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEHHCC
PVDARADAVGRSLEELGLYALGLEVTAIRRHGIRGVEPDPQTKLRASDIVVLRGLPEALA
CCCCHHHHHHCCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHH
LAEERLSRHRRDPPAVAA
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
APTNEDAPPVISPLEMTLLLLLASVVGVVVFRSLNLPPMLGYLAVGILVGPHAFGVAGD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCCCC
LERAEHLAEFGVVFLMFSIGLEFSLAKLRAMQRLVFGLGLMQVVATLALAVVLGMLFRRW
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDITWQGSVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLLIVIA
HCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHH
AFGAESSKDLALTLGFAAVKIVIALSLLLVIGQRFMTRWLNVVARRRSQELFVLNLLLVT
HHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LGAAFITDRFGLSLALGAFIAGMLIAETPFRHQVEEDIKPFRDVLLGLFFVTTGMLLDPR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
VIWEHPLLVGGFFVGQILFKATMIAGLARLFGATPGVAMRTGIGLAQAGEFGFVLLNLIL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHCCCCHHHHHHHHHH
DRHLVDATLLQAILASMLLSMLAAPFLIQNADRIVMRLSSTEWMQQSLQMTRIATQSLKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RGHVIICGYGRAGQNLARMLEQEGISYVALDLDPDRVSAAATAGESVVFGDAARRESLLA
CCCEEEEECCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEECCHHHHHHHHH
AGIHRAAAVAITYANTPSALRVLHHVHELEPTLPAIVRTVDDADLEKLLAAGATEVIPEI
HHHHHHHEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCHHHHHHHH
VEGSLMLASHTLVLVGVPMRKVVRRVEEMRDERYSLLRGYFHGADDADDDDHEQVRLQSV
HCCCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEHHCC
PVDARADAVGRSLEELGLYALGLEVTAIRRHGIRGVEPDPQTKLRASDIVVLRGLPEALA
CCCCHHHHHHCCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHH
LAEERLSRHRRDPPAVAA
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA