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The map label for this gene is dnaN [H]

Identifier: 161523182

GI number: 161523182

Start: 1817

End: 2923

Strand: Direct

Name: dnaN [H]

Synonym: Bmul_0002

Alternate gene names: 161523182

Gene position: 1817-2923 (Clockwise)

Preceding gene: 161523181

Following gene: 161523183

Centisome position: 0.05

GC content: 63.78

Gene sequence:

>1107_bases
ATGCAACTGGTCAAGACCGAACGAGACACCCTCCTCAGGCCGCTGCAAACGGTCAGCGGCATCGTCGAGCGCCGCCACAC
GTTGCCGATCCTCGCCAACCTGCTGATCACGAAGAACGGCCCGGACGTTTCTTTCCTGTCGACCGACCTGGAGCTGCAGA
TCACGACGCGCGCCGATTTCGGCGTCGGCAGCGACCAGGTGGCCACCACCGTCGCGGCCCGCAAGCTGCTCGACATCCTG
CGCGCGATGCCCGACGGCCAGGTCACGCTGTCGCTCGCGGACAAGCGCCTCACCGTTCAGTCGGGCAAGAGCCGCTTCGC
ACTCCAGACGCTCGCGGCCGACGAGTTCCCGACCGTCGCGCAGGCGAAGGATTTCGGCGCGAGTCTGACCGTCCCGCAGA
AGTCGTTCCGCCAGTTGCTCGGCATGGTGCACTTCGCGATGGCCCAGCAGGACATCCGCTACTACCTGAACGGCATGCTG
CTCGTCGTCGACGGCGATCAGCTGATGGCCGTGGCCACCGACGGCCACCGTCTCGCGTTCTCGTCGATGAAGATCGACGG
CGGCACGTTCGGGCGCCAGGAAGTGATCGTTCCGCGCAAGACCATTCTCGAGCTGCAGCGCCTGCTCGAAGACATCGACG
ACACCGTCACGATCGACATCGCCCAGACGCAGGCGAAGTTCACGTTCGGCCAGGTCGAGTTGGTGTCGAAGCTCGTCGAG
GGCAAGTTCCCCGACTTCCAGCGCGTGATTCCGAAGGCGCACAAGAACACGTTCCAGATCGGCCGCGAGGAACTGCAGCG
TTCGCTGCAACGTGCGGCGATCCTGACCTCGGACAAGTTCAAGGGCGTGCGCTGCATCGTCGCGCCGGGCCAGCTGAAGA
TCATGTCGACCAACGCCGATCAGGAAGAGGCGCAGGAAGAACTGGAAATCGCGTATCAGGGCGATACGGTCGACATCGGC
TTCAACGTCACGTATCTGCTCGACGTGCTCGCGAACCTGAAGGTCGACACCGTGCAGGTGAGCCTCGGCGACGCGAGCTC
GAGCGCACTGATCACCGTGCCCGAGAACGACGAGTTCAAGTATGTCGTGATGCCGATGCGCATCTGA

Upstream 100 bases:

>100_bases
ATACAGGTTTGACCGCCCCGGCGTCGGCGGGCAATACGTCCGGCCGCGGGGCGCTGCGAGGCTGCTGCGCTGCAGGCCGT
CATCTCAACGAAGGAACTCT

Downstream 100 bases:

>100_bases
CGCGCGCGACACACGACACATTCCAGGGGGCGGCAAGCCCCTTTGGCGTTTTTATGGCGATTCGACAACCCCTCCTTCCC
GCGCCGGGCCGGCGCCCTGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGSDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQAKDFGASLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAFSSMKIDGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
GKFPDFQRVIPKAHKNTFQIGREELQRSLQRAAILTSDKFKGVRCIVAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIG
FNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKYVVMPMRI

Sequences:

>Translated_368_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGSDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQAKDFGASLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAFSSMKIDGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
GKFPDFQRVIPKAHKNTFQIGREELQRSLQRAAILTSDKFKGVRCIVAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIG
FNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKYVVMPMRI
>Mature_368_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGSDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQAKDFGASLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAFSSMKIDGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
GKFPDFQRVIPKAHKNTFQIGREELQRSLQRAAILTSDKFKGVRCIVAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIG
FNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=363, Percent_Identity=43.801652892562, Blast_Score=302, Evalue=2e-83,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40700; Mature: 40700

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGSDQVATTVAARKLLDILRAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVA
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCEEHHHHCCCCCCCHH
QAKDFGASLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGMLLVVDGDQLMAVATDGHRLAF
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCCEEEE
SSMKIDGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
EEEEECCCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHEEHHHHHHHHHHHC
GKFPDFQRVIPKAHKNTFQIGREELQRSLQRAAILTSDKFKGVRCIVAPGQLKIMSTNAD
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCC
QEEAQEELEIAYQGDTVDIGFNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFK
HHHHHHHHEEEECCCEEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCEE
YVVMPMRI
EEEEEECC
>Mature Secondary Structure
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGSDQVATTVAARKLLDILRAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVA
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCEEHHHHCCCCCCCHH
QAKDFGASLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGMLLVVDGDQLMAVATDGHRLAF
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCCEEEE
SSMKIDGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
EEEEECCCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHEEHHHHHHHHHHHC
GKFPDFQRVIPKAHKNTFQIGREELQRSLQRAAILTSDKFKGVRCIVAPGQLKIMSTNAD
CCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCC
QEEAQEELEIAYQGDTVDIGFNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFK
HHHHHHHHEEEECCCEEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCEE
YVVMPMRI
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]