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The map label for this gene is sigE [H]

Identifier: 161486847

GI number: 161486847

Start: 1192285

End: 1192992

Strand: Direct

Name: sigE [H]

Synonym: CTC01126

Alternate gene names: 161486847

Gene position: 1192285-1192992 (Clockwise)

Preceding gene: 28210820

Following gene: 28210822

Centisome position: 42.59

GC content: 26.55

Gene sequence:

>708_bases
ATGTTTAAATTAAAAGTATTTCTAAACAAAATATTATCTAAGTTAAAAATATTTGCAAGAAATATATATTATATAGGTGG
TAGCGATGTTTTACCTCCACCTTTAACAAGAGAAGAAGAGGAAAACTTAGTAGAAAAACTTAACTATGGTGATGAGAGTA
TAAGATCTATCTTAATAGAAAGAAATTTAAGACTTGTAGTTTATATTGCAAGGAAATTTGAAAATACCGGTATAGGAATC
GAAGATTTAGTATCTGTAGGAACTATAGGTTTAATTAAAGCTGTAAATACCTTTGATCCAAGCAAAAAAATAAAATTGGC
AACCTATGCTTCAAGATGTATTGAAAATGAAATACTTATGTATCTGAGAAGAAATAATAAGATAAAATCTGAAATATCCT
TTTATGAACCATTAAATGTAGATTGGGATGGCAATGAGTTATTGCTTTCGGATATACTAGGAACTGATACTGATATTGTT
TATGATTATATAGAAGAAGAAGTAGATAAAGAATTACTTTTAATAGCTATGGATAAATTAAATAATAGGGAAAAACAAAT
AGTTAATTTAAGGTTTGGACTAGGAGGGAAAACTGAAAAAACTCAAAAGGAAGTAGCAGATATGTTGGGCATATCTCAAT
CCTATATATCTAGATTAGAAAAGCGAATAATTAAAAGATTAAAAAAGGAAATAAATAAAATGATATGA

Upstream 100 bases:

>100_bases
TTAAAAACTTTTCGTAATATCAATGGAAAAAACTATTAATAATTGTTCATTATTCATTATTAGTGATAAATGGTTGTTAA
CTATATATTGGAGGGATATT

Downstream 100 bases:

>100_bases
TTTTATTATGTATAAAAGTCGGCTTCTAAGGAAATAATTATAATGCAATTATACTAAAGGGGCTGATTTACTTATGATAA
TAAATAAAGTAGAAATATGC

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MFKLKVFLNKILSKLKIFARNIYYIGGSDVLPPPLTREEEENLVEKLNYGDESIRSILIERNLRLVVYIARKFENTGIGI
EDLVSVGTIGLIKAVNTFDPSKKIKLATYASRCIENEILMYLRRNNKIKSEISFYEPLNVDWDGNELLLSDILGTDTDIV
YDYIEEEVDKELLLIAMDKLNNREKQIVNLRFGLGGKTEKTQKEVADMLGISQSYISRLEKRIIKRLKKEINKMI

Sequences:

>Translated_235_residues
MFKLKVFLNKILSKLKIFARNIYYIGGSDVLPPPLTREEEENLVEKLNYGDESIRSILIERNLRLVVYIARKFENTGIGI
EDLVSVGTIGLIKAVNTFDPSKKIKLATYASRCIENEILMYLRRNNKIKSEISFYEPLNVDWDGNELLLSDILGTDTDIV
YDYIEEEVDKELLLIAMDKLNNREKQIVNLRFGLGGKTEKTQKEVADMLGISQSYISRLEKRIIKRLKKEINKMI
>Mature_235_residues
MFKLKVFLNKILSKLKIFARNIYYIGGSDVLPPPLTREEEENLVEKLNYGDESIRSILIERNLRLVVYIARKFENTGIGI
EDLVSVGTIGLIKAVNTFDPSKKIKLATYASRCIENEILMYLRRNNKIKSEISFYEPLNVDWDGNELLLSDILGTDTDIV
YDYIEEEVDKELLLIAMDKLNNREKQIVNLRFGLGGKTEKTQKEVADMLGISQSYISRLEKRIIKRLKKEINKMI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=250, Percent_Identity=27.6, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789871, Length=106, Percent_Identity=40.5660377358491, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27183; Mature: 27183

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLKVFLNKILSKLKIFARNIYYIGGSDVLPPPLTREEEENLVEKLNYGDESIRSILIE
CCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
RNLRLVVYIARKFENTGIGIEDLVSVGTIGLIKAVNTFDPSKKIKLATYASRCIENEILM
CCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
YLRRNNKIKSEISFYEPLNVDWDGNELLLSDILGTDTDIVYDYIEEEVDKELLLIAMDKL
HHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
NNREKQIVNLRFGLGGKTEKTQKEVADMLGISQSYISRLEKRIIKRLKKEINKMI
CCCHHHEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFKLKVFLNKILSKLKIFARNIYYIGGSDVLPPPLTREEEENLVEKLNYGDESIRSILIE
CCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
RNLRLVVYIARKFENTGIGIEDLVSVGTIGLIKAVNTFDPSKKIKLATYASRCIENEILM
CCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
YLRRNNKIKSEISFYEPLNVDWDGNELLLSDILGTDTDIVYDYIEEEVDKELLLIAMDKL
HHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
NNREKQIVNLRFGLGGKTEKTQKEVADMLGISQSYISRLEKRIIKRLKKEINKMI
CCCHHHEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883192; 11466286; 7961408 [H]