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The map label for this gene is queF [H]

Identifier: 161486732

GI number: 161486732

Start: 2182700

End: 2183530

Strand: Direct

Name: queF [H]

Synonym: Psyr_1907

Alternate gene names: 161486732

Gene position: 2182700-2183530 (Clockwise)

Preceding gene: 66045147

Following gene: 66045151

Centisome position: 35.82

GC content: 60.41

Gene sequence:

>831_bases
ATGCATCCCGCAGCCGAACACTCGCCGCTGGGCAAGTCCAGTGAATACATCGCCACCTACACGCCGTCTCTGTTGTTCCC
GATCCCGCGTGCCGCCAAATGGGCCGAGCTTGGGCTCACCGCGCAGACGCTGCCTTATCAGGGTGTCGATTTCTGGAATT
GCTACGAGCTGTCGTGGTTGCTGCCTTCCGGCAAGCCGGTGGTGGCCATTGGCGAGTTCAGTATCCCGGCCGAATCGCCG
AACATCATCGAATCGAAGTCATTCAAGCTGTACCTCAATTCGTTGAATCAGACGGCTTTCGCGACCGTCGAACAGCTGCA
GACCACCCTCGAACAGGATCTCTCGGCTGCTGCGGGCAAGCCGGTTGGCGTGCGTATTCGCAGCCTGGCGGAAATCGAAG
AGGAGGGTGTTGCTGCGTTGCCGGGCGTGTGTATCGACGACCTGGACATCTCGGTCAGCAGCTATGACCGGCCGCAGCCA
GAGTTGCTGTGTTGTGATGACTCGCGGGTGGTGGCAGAGAGCGTGCACAGTCATCTGCTCAAATCCAACTGTCCGGTCAC
CAGCCAGCCTGACTGGGGCAGCGTAGTGGTCGAATATCGCGGCGCAGCGCTGGATCACGCCAGCCTGCTGGCGTATATCG
TCAGCTTCCGCCAGCACTCGGATTTTCATGAACAGTGCGTGGAAAGAATCTTCCTCGACCTGCAGCGCCTGCTCAAGCCG
GAGAAGCTCACCGTGTATGCGCGCTACGTGCGCCGCGGTGGTCTGGACATCAACCCGTACCGCAGCACTGAAACGCTCGA
CGTAGACAACCGCAGGCTGGCGCGGCAGTAG

Upstream 100 bases:

>100_bases
AGGGCGGCCTGCGAGGGTGGCGGGGCAGAGCGGATTTTCGCTGAAAGCCCCAGGCAAACGCTATAAACTCGCCGCGTTCA
TTCATGATCCAGGTATCGCC

Downstream 100 bases:

>100_bases
TGTCTGAGGCCCGCGGAGCGGACGCAGAGCGTCCGGAGCGACATTCCCACGCGGAGCGTGAGGAACGATAGGTGTCAGTA
GTGCCGATGCTCCGCGTCGC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRAAKWAELGLTAQTLPYQGVDFWNCYELSWLLPSGKPVVAIGEFSIPAESP
NIIESKSFKLYLNSLNQTAFATVEQLQTTLEQDLSAAAGKPVGVRIRSLAEIEEEGVAALPGVCIDDLDISVSSYDRPQP
ELLCCDDSRVVAESVHSHLLKSNCPVTSQPDWGSVVVEYRGAALDHASLLAYIVSFRQHSDFHEQCVERIFLDLQRLLKP
EKLTVYARYVRRGGLDINPYRSTETLDVDNRRLARQ

Sequences:

>Translated_276_residues
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRAAKWAELGLTAQTLPYQGVDFWNCYELSWLLPSGKPVVAIGEFSIPAESP
NIIESKSFKLYLNSLNQTAFATVEQLQTTLEQDLSAAAGKPVGVRIRSLAEIEEEGVAALPGVCIDDLDISVSSYDRPQP
ELLCCDDSRVVAESVHSHLLKSNCPVTSQPDWGSVVVEYRGAALDHASLLAYIVSFRQHSDFHEQCVERIFLDLQRLLKP
EKLTVYARYVRRGGLDINPYRSTETLDVDNRRLARQ
>Mature_276_residues
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRAAKWAELGLTAQTLPYQGVDFWNCYELSWLLPSGKPVVAIGEFSIPAESP
NIIESKSFKLYLNSLNQTAFATVEQLQTTLEQDLSAAAGKPVGVRIRSLAEIEEEGVAALPGVCIDDLDISVSSYDRPQP
ELLCCDDSRVVAESVHSHLLKSNCPVTSQPDWGSVVVEYRGAALDHASLLAYIVSFRQHSDFHEQCVERIFLDLQRLLKP
EKLTVYARYVRRGGLDINPYRSTETLDVDNRRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG2904

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=277, Percent_Identity=50.5415162454874, Blast_Score=277, Evalue=7e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 30703; Mature: 30703

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRAAKWAELGLTAQTLPYQGVDFWNCYELSWL
CCCCCCCCCCCCCCCEEEEECCHHEECCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEE
LPSGKPVVAIGEFSIPAESPNIIESKSFKLYLNSLNQTAFATVEQLQTTLEQDLSAAAGK
CCCCCCEEEEECEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
PVGVRIRSLAEIEEEGVAALPGVCIDDLDISVSSYDRPQPELLCCDDSRVVAESVHSHLL
CHHHHHHHHHHHHHCCCHHCCCCEECCCCCEECCCCCCCCCEEEECCCHHHHHHHHHHHH
KSNCPVTSQPDWGSVVVEYRGAALDHASLLAYIVSFRQHSDFHEQCVERIFLDLQRLLKP
HCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EKLTVYARYVRRGGLDINPYRSTETLDVDNRRLARQ
CHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHCCC
>Mature Secondary Structure
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRAAKWAELGLTAQTLPYQGVDFWNCYELSWL
CCCCCCCCCCCCCCCEEEEECCHHEECCCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEE
LPSGKPVVAIGEFSIPAESPNIIESKSFKLYLNSLNQTAFATVEQLQTTLEQDLSAAAGK
CCCCCCEEEEECEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
PVGVRIRSLAEIEEEGVAALPGVCIDDLDISVSSYDRPQPELLCCDDSRVVAESVHSHLL
CHHHHHHHHHHHHHCCCHHCCCCEECCCCCEECCCCCCCCCEEEECCCHHHHHHHHHHHH
KSNCPVTSQPDWGSVVVEYRGAALDHASLLAYIVSFRQHSDFHEQCVERIFLDLQRLLKP
HCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EKLTVYARYVRRGGLDINPYRSTETLDVDNRRLARQ
CHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA