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The map label for this gene is rffA [H]

Identifier: 161486669

GI number: 161486669

Start: 313619

End: 314752

Strand: Direct

Name: rffA [H]

Synonym: VV0305

Alternate gene names: 161486669

Gene position: 313619-314752 (Clockwise)

Preceding gene: 37678487

Following gene: 37678490

Centisome position: 9.35

GC content: 37.04

Gene sequence:

>1134_bases
ATGATTCCATTCAATAAACCTCCAGTTACTGGTGAAGAACTTCAATTTATTCAGGAAGCTATTTCTTGTGGGAAGCTTTC
GGGAGATGGAGAATTCTCAAGAAAGTGCGAAAATTGGTTTGTATCACATACTGGTACGACTAAAGCTCTTCTTACTCCTT
CATGTACTCATGCGCTAGAAATGGCTGCGATGCTCATCGATATCCAGCCTGGTGATGAAGTAATAATGCCAAGTTATACA
TTCGTTAGTACAGCCAACGCTTTTGTATTAAGAGGGGCCAAAGTAGTATTTGTGGACATTCGAGCTGATACGATGAATAT
CGACGAATCCAAGATAGAGGTAGCTATCACCGAGCGCACTAAGGCGATCGTGCCCGTACATTATGCAGGGGTGGCTTGTG
ATATGGACACTATAATGGGTTTATCTGACAAATATGGTTTGTATGTTATTGAAGATGCAGCCCAAGGGATGATGTCAACT
TATAAAGGTCGAGCGTTAGGTAGTATTGGTCATATAGGGACCTATAGTTTTCATGAAACCAAAAATTATACGAGTGGTGG
TGAAGGTGGTTTACTGTTGGTTAATGATCAGAGTTTTTCTCAACGAGCAGAAATAATTAGAGAAAAAGGCACTAATCGCA
ATCAGTTTTTTAGAGGTATGGTTGATAAATATACATGGGTAGATGTTGGTAGTAGTATGTTACCTAGTGAAATTCAATCT
GCTTATTTATGGGCCCAGTTGATTAATGCTAAAAGAATTAATGAGAAGAGAATTAATGATTGGAATTACTATTTTGATAG
TTTGTTACCCTTGAAGGAAAAAGGATTAATTGAGTTGCCCAACATTCCAATTGAATGTACACACAATGCTCATATGTTCT
ACATTAAACTACCGGACTTAACCCAAAGAACAAGGTTTATTGAAGCCATGTTTAAACAAGGTGTTCTTTCTGTTTTTCAT
TATATACCATTACATTCTTCGCCTGCAGGAAAAAGTTTTGGTCGATTCAATGGTAAGGATATCTATACAACCATGGAAAG
TGAAAAATTAGTTCGCTTACCTATGTTTTATGGATTGGCTCTCTCAGAGAGAAATAAGATCATATCTACTATTTTTGATA
TTTTGGAGTCTTAA

Upstream 100 bases:

>100_bases
CTCTGCTACTAACTATACATCAATTTCGGTGGTAGTATAGTACCTACAAGCTAGGGTACTAGTTTCGGTAGTATGTTGAT
AAATGTTTGAGACAAATTAT

Downstream 100 bases:

>100_bases
TGTTTCAAGATAAACATGTGTTTTTTGTAGATGTTGAAGATAATTTTATATATTTAAATGTAGATGCTTTGATTTATAGG
GCGGGCGTTGAACCTAGTAA

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MIPFNKPPVTGEELQFIQEAISCGKLSGDGEFSRKCENWFVSHTGTTKALLTPSCTHALEMAAMLIDIQPGDEVIMPSYT
FVSTANAFVLRGAKVVFVDIRADTMNIDESKIEVAITERTKAIVPVHYAGVACDMDTIMGLSDKYGLYVIEDAAQGMMST
YKGRALGSIGHIGTYSFHETKNYTSGGEGGLLLVNDQSFSQRAEIIREKGTNRNQFFRGMVDKYTWVDVGSSMLPSEIQS
AYLWAQLINAKRINEKRINDWNYYFDSLLPLKEKGLIELPNIPIECTHNAHMFYIKLPDLTQRTRFIEAMFKQGVLSVFH
YIPLHSSPAGKSFGRFNGKDIYTTMESEKLVRLPMFYGLALSERNKIISTIFDILES

Sequences:

>Translated_377_residues
MIPFNKPPVTGEELQFIQEAISCGKLSGDGEFSRKCENWFVSHTGTTKALLTPSCTHALEMAAMLIDIQPGDEVIMPSYT
FVSTANAFVLRGAKVVFVDIRADTMNIDESKIEVAITERTKAIVPVHYAGVACDMDTIMGLSDKYGLYVIEDAAQGMMST
YKGRALGSIGHIGTYSFHETKNYTSGGEGGLLLVNDQSFSQRAEIIREKGTNRNQFFRGMVDKYTWVDVGSSMLPSEIQS
AYLWAQLINAKRINEKRINDWNYYFDSLLPLKEKGLIELPNIPIECTHNAHMFYIKLPDLTQRTRFIEAMFKQGVLSVFH
YIPLHSSPAGKSFGRFNGKDIYTTMESEKLVRLPMFYGLALSERNKIISTIFDILES
>Mature_377_residues
MIPFNKPPVTGEELQFIQEAISCGKLSGDGEFSRKCENWFVSHTGTTKALLTPSCTHALEMAAMLIDIQPGDEVIMPSYT
FVSTANAFVLRGAKVVFVDIRADTMNIDESKIEVAITERTKAIVPVHYAGVACDMDTIMGLSDKYGLYVIEDAAQGMMST
YKGRALGSIGHIGTYSFHETKNYTSGGEGGLLLVNDQSFSQRAEIIREKGTNRNQFFRGMVDKYTWVDVGSSMLPSEIQS
AYLWAQLINAKRINEKRINDWNYYFDSLLPLKEKGLIELPNIPIECTHNAHMFYIKLPDLTQRTRFIEAMFKQGVLSVFH
YIPLHSSPAGKSFGRFNGKDIYTTMESEKLVRLPMFYGLALSERNKIISTIFDILES

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=373, Percent_Identity=64.6112600536193, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI145693159, Length=384, Percent_Identity=30.2083333333333, Blast_Score=175, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42349; Mature: 42349

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFNKPPVTGEELQFIQEAISCGKLSGDGEFSRKCENWFVSHTGTTKALLTPSCTHALE
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHH
MAAMLIDIQPGDEVIMPSYTFVSTANAFVLRGAKVVFVDIRADTMNIDESKIEVAITERT
HHHHEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEEECCCCCCCCCEEEEEEECCC
KAIVPVHYAGVACDMDTIMGLSDKYGLYVIEDAAQGMMSTYKGRALGSIGHIGTYSFHET
CEEEEEEECCEEECHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCEECCC
KNYTSGGEGGLLLVNDQSFSQRAEIIREKGTNRNQFFRGMVDKYTWVDVGSSMLPSEIQS
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCEEEEECCCCCCHHHHHH
AYLWAQLINAKRINEKRINDWNYYFDSLLPLKEKGLIELPNIPIECTHNAHMFYIKLPDL
HHHHHHHHHHHHCCHHCCCCHHHHHHHCCCCCCCCCEECCCCCEEECCCCEEEEEECCCH
TQRTRFIEAMFKQGVLSVFHYIPLHSSPAGKSFGRFNGKDIYTTMESEKLVRLPMFYGLA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECHHHHCEE
LSERNKIISTIFDILES
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPFNKPPVTGEELQFIQEAISCGKLSGDGEFSRKCENWFVSHTGTTKALLTPSCTHALE
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEECCCCCEEEECCCHHHHHH
MAAMLIDIQPGDEVIMPSYTFVSTANAFVLRGAKVVFVDIRADTMNIDESKIEVAITERT
HHHHEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEEECCCCCCCCCEEEEEEECCC
KAIVPVHYAGVACDMDTIMGLSDKYGLYVIEDAAQGMMSTYKGRALGSIGHIGTYSFHET
CEEEEEEECCEEECHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCEECCC
KNYTSGGEGGLLLVNDQSFSQRAEIIREKGTNRNQFFRGMVDKYTWVDVGSSMLPSEIQS
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCEEEEECCCCCCHHHHHH
AYLWAQLINAKRINEKRINDWNYYFDSLLPLKEKGLIELPNIPIECTHNAHMFYIKLPDL
HHHHHHHHHHHHCCHHCCCCHHHHHHHCCCCCCCCCEECCCCCEEECCCCEEEEEECCCH
TQRTRFIEAMFKQGVLSVFHYIPLHSSPAGKSFGRFNGKDIYTTMESEKLVRLPMFYGLA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECHHHHCEE
LSERNKIISTIFDILES
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]