Click here to switch to the map view.

The map label for this gene is kdsA

Identifier: 161486643

GI number: 161486643

Start: 934595

End: 935449

Strand: Direct

Name: kdsA

Synonym: VV0935

Alternate gene names: 161486643

Gene position: 934595-935449 (Clockwise)

Preceding gene: 37679118

Following gene: 37679120

Centisome position: 27.86

GC content: 48.07

Gene sequence:

>855_bases
ATGATGGAACAGAAAATTGTTCAGATTGGTGATATTCCAGTTGCCAACGATAAGCCATTTACTTTGTTTGCTGGCATGAA
CGTACTAGAGTCACGTGATTTGGCGATGCAGATCTGTGAGCACTATGTGAAAGTGACCGATAAGTTGGGTATTCCTTACG
TATTCAAAGCATCTTTTGATAAAGCCAACCGCAGTTCTGTTCACTCATACCGTGGCCCTGGTCTTGAAGAAGGGATGAAA
ATCTTCCAAGAGCTGAAAGACACGTTTGGCGTAAAGATCATCACCGATGTTCACACGGAAGCACAAGCGCAGCCTGTAGC
GGATGTGGTGGATGTTATCCAACTACCTGCGTTCTTGGCTCGCCAGACTGACCTTGTGGAAGCAATGGCCAAAACGGGCG
CGGTAATCAACGTGAAGAAACCTCAGTTCATGAGCCCAGGTCAAGTGGGCAACATCGTTGAGAAATTTGCGGAGTGTGGC
AACGATAAGATCATTCTTTGTGAGCGTGGTTCTTGTCATGGTTACGATAACCTAGTCGTAGATATGCTGGGTTTTGGCGT
AATGAAAAAAGCGTCAAAAGGTAGCCCAATCATTTTTGATGTGACGCACTCACTGCAAATGCGCGACCCATCAGGCGCGG
CTTCAGGCGGTCGCCGTGAACAGACCGTTGAACTAGCGAAAGCCGGTTTGGCAACGGGTATTGCTGGTCTGTTTATTGAG
GCGCACCCAAATCCAGACCAAGCACGTTGTGATGGCCCATCGGCATTGCCACTCGACAAACTAGAGCCGTTCCTTGCGCA
AATGAAAGCACTGGACGATCTTGTGAAAAGCTTCGCGAGTATCGATATCCGATAA

Upstream 100 bases:

>100_bases
GATCAGTGTCCCGATGACCCAGCGGCAGAGCTGCTAAAAAAACAAGTTAACGTGATGAGTGGCAAAGCGGTCACTCTACA
TTGATCAAATAAGAGACATA

Downstream 100 bases:

>100_bases
TATTTTCGTGTTATCGAGATTAAAAAAAGGACCGAGAAAACGGTCCTTTTTTGTTTTTTCCGTCGGCTTCTCTTTCGTGA
TGTGGTGGCTTTTTATTCTG

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MMEQKIVQIGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFDKANRSSVHSYRGPGLEEGMK
IFQELKDTFGVKIITDVHTEAQAQPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECG
NDKIILCERGSCHGYDNLVVDMLGFGVMKKASKGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIE
AHPNPDQARCDGPSALPLDKLEPFLAQMKALDDLVKSFASIDIR

Sequences:

>Translated_284_residues
MMEQKIVQIGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFDKANRSSVHSYRGPGLEEGMK
IFQELKDTFGVKIITDVHTEAQAQPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECG
NDKIILCERGSCHGYDNLVVDMLGFGVMKKASKGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIE
AHPNPDQARCDGPSALPLDKLEPFLAQMKALDDLVKSFASIDIR
>Mature_284_residues
MMEQKIVQIGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFDKANRSSVHSYRGPGLEEGMK
IFQELKDTFGVKIITDVHTEAQAQPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECG
NDKIILCERGSCHGYDNLVVDMLGFGVMKKASKGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIE
AHPNPDQARCDGPSALPLDKLEPFLAQMKALDDLVKSFASIDIR

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=281, Percent_Identity=81.1387900355872, Blast_Score=476, Evalue=1e-136,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_VIBVU (Q8DFG3)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759258.1
- ProteinModelPortal:   Q8DFG3
- SMR:   Q8DFG3
- GeneID:   1177202
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0249
- HOGENOM:   HBG575990
- OMA:   QLILCDR
- ProtClustDB:   PRK05198
- BioCyc:   VVUL216895:VV1_0249-MONOMER
- BRENDA:   2.5.1.55
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 30851; Mature: 30851

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEQKIVQIGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFD
CCCCCEEHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
KANRSSVHSYRGPGLEEGMKIFQELKDTFGVKIITDVHTEAQAQPVADVVDVIQLPAFLA
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHCCHHHHHHHHHHHHHHHH
RQTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECGNDKIILCERGSCHGYDNLVV
HHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
DMLGFGVMKKASKGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIE
HHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEE
AHPNPDQARCDGPSALPLDKLEPFLAQMKALDDLVKSFASIDIR
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMEQKIVQIGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFD
CCCCCEEHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
KANRSSVHSYRGPGLEEGMKIFQELKDTFGVKIITDVHTEAQAQPVADVVDVIQLPAFLA
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHCCHHHHHHHHHHHHHHHH
RQTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECGNDKIILCERGSCHGYDNLVV
HHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
DMLGFGVMKKASKGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIE
HHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEE
AHPNPDQARCDGPSALPLDKLEPFLAQMKALDDLVKSFASIDIR
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA