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The map label for this gene is ushA

Identifier: 37679120

GI number: 37679120

Start: 935821

End: 937515

Strand: Direct

Name: ushA

Synonym: VV0936

Alternate gene names: 37679120

Gene position: 935821-937515 (Clockwise)

Preceding gene: 161486643

Following gene: 37679121

Centisome position: 27.9

GC content: 47.73

Gene sequence:

>1695_bases
GTGGAATCCCCAAAAAGTTCAAAAGGTATGACAATGAAGCAACGCCTGATTGTAAAAACCGCGCTTAGCGCAGCTATTCT
GGCAACTCTGGCGGGTTGTGCAACTCAGCCAACTCAAGAATGGGCAGCAGATACAACTTACAAACTGACAGTACTGCACA
CAAACGACCACCACGGCCGTTTCTGGCAAAACAAATACGGTGAGTACGGTATGGCAGCTCGCAAAACTCTGATCGATGAG
TTGCGTGCTGAAATCCAAGCGGAAGGCGGCAGCGTACTGCTACTTTCAGGTGGTGATATCAACACAGGTGTTCCTGAGTC
AGACCTTCAAGATGCTGAGCCAGATTTCAAAGGCATGAGCAAAATCGGTTACGACGCAATGGCGCTGGGTAACCACGAGT
TTGACAATCCTCTAGACGTACTGATGAAGCAGAAAGAGTGGGCTAACTTCCCAATGCTGTCTGCCAACATTTACGACAAG
AAAACCGGCGAGCGCATGTTCCAAGCTTACGAGATGTTCGATAAGCAAGGTATCAAAATTGCGGTTATCGGTCTAACAAC
CGAAGATACAGCTAAGCTAGGCAACCCAGAATTCATCGGCGCAATTGACTTCCGTGATCCGAAGGAAGAAGCGAAGAAGC
TGATTGCTGAGCTAAAAGAAACAGAAAAACCAGATTTGATCTTCGCCGTGACGCACATGGGTCACTACGAAGATGGTAAG
CGTGGCATCAACGCTCCTGGCGATGTGGCACTGGCTCGTTACCTAAACGAAGGTGACCTAGACATGATCGTCGGTGGTCA
CTCGCAAGAGCCGGTTTGTATGGAAGCACCAAATGTGGTGAAGAAAAACTTCAAACCAGCAGACGAGTGTAAGCCAGACC
AACAAAACGGCACTTACATCGTGCAAGCGCACGAGTGGGGTAAATACGTTGGCCGTGCTGATTACGAGTTCCGCAATGGT
GAGCTACGCATGGTGAGCTACGATCTGATTCCGGTTAACCTGAAGAAGAAAGTGGAAGTCGATGGTAAGAGCCAGCGCGT
CTTCATTGAAAGCGAAATTAAAGAAGATACTGCATTGCTAGAGTTCCTACGTCCTTACCAAGAGAAAGGTCAAGAGCAAC
TTAACGTGAAGATTGCAGACACAAACGGCAAGCTTGAAGGTGACCGTAACGTGGTTCGCTTCCAGCAAACTAACCTAGGT
CGTTTGATCGCAGTATCACACATGGAGCGTGCAAAAGCAGACTTTGCAGTAATGAACTCGGGTGGTGTACGTGACTCGAT
TGAAGCGGGTGAAGTGACTTACAAAGACGTTCTTACTGTTCAGCCTTTTGCAAACATCCTGACTTACACTGATATGACGG
GTAAAGAAGTTCTGGATTACCTAAACGTTGTTGCAACGAAACCTGTTGATTCAGGTGCTTATGCGCAATTCGCTGGTATC
TCAATGACAGTGGCTAACGGCAAAGTATCGAACGTATTCATTGGTGGCAAACAGCTTCGTCTAGATGAAACTTACCGCTT
CACCGTACCAAGCTACAACGCCGCGGGTGGTGATGGTTACCCTAAACTGACTGGCCACCCTGGTTACGTGAACACTGGTT
TTGTGGATGCTGAAGTACTGAAAGAGTTCCTAGAGAAGAACAGCCCAATCGATGTGAACAAGTTCGCGCCAAACGGTGAG
ATTGTTTACAAATAA

Upstream 100 bases:

>100_bases
TGGTTTGGCCAGTTACTTTAAACTGTATCAAGTTTTACAGCCAACCTAACTCGGTTTAATAAATTGAGCTTATGTTTAAA
AAGGCCTGTAGATATTTGCA

Downstream 100 bases:

>100_bases
TCGATAAGAGAAAGAGTTTGACTCAATCTCGCTGATATCTTTAACTAAAGCGCTATTTCGATCTGGAATAGCGCTTTTTG
TTTATACAGGAGAAAGACGA

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MESPKSSKGMTMKQRLIVKTALSAAILATLAGCATQPTQEWAADTTYKLTVLHTNDHHGRFWQNKYGEYGMAARKTLIDE
LRAEIQAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGNHEFDNPLDVLMKQKEWANFPMLSANIYDK
KTGERMFQAYEMFDKQGIKIAVIGLTTEDTAKLGNPEFIGAIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYEDGK
RGINAPGDVALARYLNEGDLDMIVGGHSQEPVCMEAPNVVKKNFKPADECKPDQQNGTYIVQAHEWGKYVGRADYEFRNG
ELRMVSYDLIPVNLKKKVEVDGKSQRVFIESEIKEDTALLEFLRPYQEKGQEQLNVKIADTNGKLEGDRNVVRFQQTNLG
RLIAVSHMERAKADFAVMNSGGVRDSIEAGEVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
SMTVANGKVSNVFIGGKQLRLDETYRFTVPSYNAAGGDGYPKLTGHPGYVNTGFVDAEVLKEFLEKNSPIDVNKFAPNGE
IVYK

Sequences:

>Translated_564_residues
MESPKSSKGMTMKQRLIVKTALSAAILATLAGCATQPTQEWAADTTYKLTVLHTNDHHGRFWQNKYGEYGMAARKTLIDE
LRAEIQAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGNHEFDNPLDVLMKQKEWANFPMLSANIYDK
KTGERMFQAYEMFDKQGIKIAVIGLTTEDTAKLGNPEFIGAIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYEDGK
RGINAPGDVALARYLNEGDLDMIVGGHSQEPVCMEAPNVVKKNFKPADECKPDQQNGTYIVQAHEWGKYVGRADYEFRNG
ELRMVSYDLIPVNLKKKVEVDGKSQRVFIESEIKEDTALLEFLRPYQEKGQEQLNVKIADTNGKLEGDRNVVRFQQTNLG
RLIAVSHMERAKADFAVMNSGGVRDSIEAGEVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
SMTVANGKVSNVFIGGKQLRLDETYRFTVPSYNAAGGDGYPKLTGHPGYVNTGFVDAEVLKEFLEKNSPIDVNKFAPNGE
IVYK
>Mature_564_residues
MESPKSSKGMTMKQRLIVKTALSAAILATLAGCATQPTQEWAADTTYKLTVLHTNDHHGRFWQNKYGEYGMAARKTLIDE
LRAEIQAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGNHEFDNPLDVLMKQKEWANFPMLSANIYDK
KTGERMFQAYEMFDKQGIKIAVIGLTTEDTAKLGNPEFIGAIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYEDGK
RGINAPGDVALARYLNEGDLDMIVGGHSQEPVCMEAPNVVKKNFKPADECKPDQQNGTYIVQAHEWGKYVGRADYEFRNG
ELRMVSYDLIPVNLKKKVEVDGKSQRVFIESEIKEDTALLEFLRPYQEKGQEQLNVKIADTNGKLEGDRNVVRFQQTNLG
RLIAVSHMERAKADFAVMNSGGVRDSIEAGEVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
SMTVANGKVSNVFIGGKQLRLDETYRFTVPSYNAAGGDGYPKLTGHPGYVNTGFVDAEVLKEFLEKNSPIDVNKFAPNGE
IVYK

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=550, Percent_Identity=23.6363636363636, Blast_Score=106, Evalue=7e-23,
Organism=Escherichia coli, GI1786687, Length=549, Percent_Identity=61.0200364298725, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=490, Percent_Identity=23.265306122449, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922446, Length=507, Percent_Identity=26.6272189349112, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24654424, Length=507, Percent_Identity=26.6272189349112, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922444, Length=567, Percent_Identity=23.9858906525573, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28573524, Length=526, Percent_Identity=25.6653992395437, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19921980, Length=507, Percent_Identity=26.4299802761341, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24652512, Length=507, Percent_Identity=26.4299802761341, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161076508, Length=507, Percent_Identity=26.4299802761341, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24641187, Length=549, Percent_Identity=23.1329690346084, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI221329836, Length=556, Percent_Identity=21.7625899280576, Blast_Score=95, Evalue=2e-19,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 62497; Mature: 62497

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESPKSSKGMTMKQRLIVKTALSAAILATLAGCATQPTQEWAADTTYKLTVLHTNDHHGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEEEECCCCCC
FWQNKYGEYGMAARKTLIDELRAEIQAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMS
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHH
KIGYDAMALGNHEFDNPLDVLMKQKEWANFPMLSANIYDKKTGERMFQAYEMFDKQGIKI
HCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEE
AVIGLTTEDTAKLGNPEFIGAIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYEDGK
EEEEECCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
RGINAPGDVALARYLNEGDLDMIVGGHSQEPVCMEAPNVVKKNFKPADECKPDQQNGTYI
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCHHCCCCCCCCCEE
VQAHEWGKYVGRADYEFRNGELRMVSYDLIPVNLKKKVEVDGKSQRVFIESEIKEDTALL
EEECHHHHHCCCCCCEEECCEEEEEEEEEEEECCCEEEEECCCCCEEEEECHHHHHHHHH
EFLRPYQEKGQEQLNVKIADTNGKLEGDRNVVRFQQTNLGRLIAVSHMERAKADFAVMNS
HHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHCCCEEEECC
GGVRDSIEAGEVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
CCCCCCCCCCCEEHHHEEEECCHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCEEECCE
SMTVANGKVSNVFIGGKQLRLDETYRFTVPSYNAAGGDGYPKLTGHPGYVNTGFVDAEVL
EEEEECCCEEEEEECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KEFLEKNSPIDVNKFAPNGEIVYK
HHHHHCCCCCCEEEECCCCCEEEC
>Mature Secondary Structure
MESPKSSKGMTMKQRLIVKTALSAAILATLAGCATQPTQEWAADTTYKLTVLHTNDHHGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEEEECCCCCC
FWQNKYGEYGMAARKTLIDELRAEIQAEGGSVLLLSGGDINTGVPESDLQDAEPDFKGMS
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHH
KIGYDAMALGNHEFDNPLDVLMKQKEWANFPMLSANIYDKKTGERMFQAYEMFDKQGIKI
HCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEE
AVIGLTTEDTAKLGNPEFIGAIDFRDPKEEAKKLIAELKETEKPDLIFAVTHMGHYEDGK
EEEEECCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
RGINAPGDVALARYLNEGDLDMIVGGHSQEPVCMEAPNVVKKNFKPADECKPDQQNGTYI
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCHHCCCCCCCCCEE
VQAHEWGKYVGRADYEFRNGELRMVSYDLIPVNLKKKVEVDGKSQRVFIESEIKEDTALL
EEECHHHHHCCCCCCEEECCEEEEEEEEEEEECCCEEEEECCCCCEEEEECHHHHHHHHH
EFLRPYQEKGQEQLNVKIADTNGKLEGDRNVVRFQQTNLGRLIAVSHMERAKADFAVMNS
HHHHHHHHCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEHHHHHHCCCEEEECC
GGVRDSIEAGEVTYKDVLTVQPFANILTYTDMTGKEVLDYLNVVATKPVDSGAYAQFAGI
CCCCCCCCCCCEEHHHEEEECCHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCEEECCE
SMTVANGKVSNVFIGGKQLRLDETYRFTVPSYNAAGGDGYPKLTGHPGYVNTGFVDAEVL
EEEEECCCEEEEEECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
KEFLEKNSPIDVNKFAPNGEIVYK
HHHHHCCCCCCEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA