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The map label for this gene is metG

Identifier: 161485679

GI number: 161485679

Start: 1024811

End: 1026958

Strand: Direct

Name: metG

Synonym: MM_0867

Alternate gene names: 161485679

Gene position: 1024811-1026958 (Clockwise)

Preceding gene: 21226966

Following gene: 21226975

Centisome position: 25.02

GC content: 48.84

Gene sequence:

>2148_bases
ATGGAGATAAGAATGTCAAAATCATCCAGTAAACCCGTACTTGTCACATGCGGCCTGCCCTATGCCAATGGCAAGGCACA
TATCGGGCATCTCCGGACCTATGTGCCCGCTGATATTTATGCCCGTTCCCTGAAAAAAGAGGGGAGGGAAGTAACCTTTG
TTTGTGGCTCGGACACCCATGGAACCCCTATTGTCGTAAACGCCGAAGAACTGGGAATTACCCCAACAGAACTTGTAGAA
ATATATCACAAACACTTTGACGAAACTTTCAAGCAGCTTGGAATTTATTTTGATGCTTTCGGGACAACGGATGATCCTGA
AAACCACAACCGGACCCACGACATAGTGAGCAGGCTGATTGAAAAAGGATATGTCTACCCGAAAATTATAGAAATCGCCT
ACTGTCCGGCATGCAACCGCTTCCTTCCTGACCGTTATGTAGAAGGAGCCTGCCCTCACTGCGGAGAAACTGCCAGAGGA
GATGAATGCGACCAGGGCTGCGGAAAACATCTTGAGCCCGGGGAGCTTCAGAACCCTGTATGTACCATCTGCGGAGGCCC
TGCAGAGTACCGCCAGCAGGAACACTTCTTTTTCAAGCTTTCCGAGTTCAGCAACTACCTGATGGATTACCTCTCAAAAG
ACCTCGGAGGGACCACAAATGCCATCAACTACGCACGCGGCTGGGTAAAGCAGGGGCTTACGGACTGGTGCATTACACGG
AACCTTGAGTGGGGAGTTAAATTCCCGGGACACGAAGACCTTGTGGTTTATGTCTGGGTGGACGCTCCGATAGGTTATAT
CGCTTTTACCGAGGAATGGGCTGCACAGGCAGGAGACTCCTGGGAAAAGTTCTGGAAGGGAGACGGGGAAATAGTCCACT
TTATAGGAGGGGACATAACCTATCACCATTGCATCTTCTGGCCTGCCATGCTTAAAGGCGCAGATTACTCCGTGCCAACA
GCAGTTGTAGCTTCAGGGATGGTAAAAATCGAAGACAGGAAGTTCTCGAAAACCAGAGGCTATGTAGTCTGGGTTGGGGA
AGACTATCTTGACCACGGCTTCCATCCCGACCTTCTCAGGTATTACCTTGCAAGCTATACCTCCCACACCAAGGAACTGA
ACTTCTCCTGGCGCGTGCTTCAGGAAAAGATCAATGCCGAACTGGTTGCAGTTCTCGGAAACTTCCTTTACAGGACCATG
CTCTTTGCTTTCAAGAACTACGGAGAAGTCCCGGAAGGTGAAGTAGAGCCTGAAATCCGGAAAGAGATAGAAAAAGCTCT
CGTAGAGGTCAAAGCTGCAATGGAAGAGTACGAGTTCAAGAAAGCAGTGGACTCTGCCATGGCTCTTGCATCTTTCGGTA
ACACTTATTTCCAGTCCCATGAACCCTGGAAACTGATAAAAGAAGACAGGGATGCATGCGGACAGGTCGTCTATAACTGC
CTGCACCTGGCAAAAGCCCTGAGCCTGATATTTGAACCCGTTATCCCACAGACAATGGAGAAAGCCTGGAAAGGGCTAGG
GCACGAGAGCGACATACATGCCGCACTTTATGAAGAGACCCTTGTGCCCCTGAAAGCAGGCACAAAACTTGCAAAACCTG
AAATCCTCTTTACCAAGCTCGAAGATGACAGGATAGGAGAGATGGAAGAGATTGCAAACCAGCGTGTAGCAGCCGCAAAT
GCAAAGAGAAACGGAGTAAAAGGAGGCGAAAAAGAGCCTTCAAAGTCCGAAGGAATGGGACCCTCTGAAGCATCAAAAGC
ATCCGAAAAAACAGTAGATGTTGCAACAGCAGAAGAGAAAGTACAGGTAGAAACCCTTCCAGTGATCGATTACGAGGACT
TTGCAAAACTTGACATAAGGGTAGGAAAAGTACTCCTCGTCGAACCTGTGAAAAAGTCAAAAAAGCTCCTGAGGATTGAA
GTGGATATAGGAGAGGAAAAGCCCAGGCAGCTTGTTGCAGGCATGGCTTCATATTACACTCCAGAAGAGCTTGTAGGCAA
ATCCGTAATCGTACTCGCCAACCTGAAACCTGCCAAACTCTGTGGAGTTGAGTCAAACGGTATGATGCTTGCAGCCGATG
ACGGCGGAGCAATTGTGGCAGCCCTGATGCCGGATAAAGAGATTAAGTCGGGTTCAAGGATAAGGTAA

Upstream 100 bases:

>100_bases
GATAATCAACTATCCCTCAAATAAGTTCCCTTGTAAACACGATAAGCGAAATATATTTCAGAATTGCAGTCATCCAGAAG
AGATACATCTAGAAAAATAT

Downstream 100 bases:

>100_bases
AGATTGAAAAAAAGTTAGAATCGGGTAAAATCAAAATAATCAGGTAAAATCAAAATAACATAGAAGCTTAAACCCGGCTT
TAGACAGGTTAAAGTTTGAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MEIRMSKSSSKPVLVTCGLPYANGKAHIGHLRTYVPADIYARSLKKEGREVTFVCGSDTHGTPIVVNAEELGITPTELVE
IYHKHFDETFKQLGIYFDAFGTTDDPENHNRTHDIVSRLIEKGYVYPKIIEIAYCPACNRFLPDRYVEGACPHCGETARG
DECDQGCGKHLEPGELQNPVCTICGGPAEYRQQEHFFFKLSEFSNYLMDYLSKDLGGTTNAINYARGWVKQGLTDWCITR
NLEWGVKFPGHEDLVVYVWVDAPIGYIAFTEEWAAQAGDSWEKFWKGDGEIVHFIGGDITYHHCIFWPAMLKGADYSVPT
AVVASGMVKIEDRKFSKTRGYVVWVGEDYLDHGFHPDLLRYYLASYTSHTKELNFSWRVLQEKINAELVAVLGNFLYRTM
LFAFKNYGEVPEGEVEPEIRKEIEKALVEVKAAMEEYEFKKAVDSAMALASFGNTYFQSHEPWKLIKEDRDACGQVVYNC
LHLAKALSLIFEPVIPQTMEKAWKGLGHESDIHAALYEETLVPLKAGTKLAKPEILFTKLEDDRIGEMEEIANQRVAAAN
AKRNGVKGGEKEPSKSEGMGPSEASKASEKTVDVATAEEKVQVETLPVIDYEDFAKLDIRVGKVLLVEPVKKSKKLLRIE
VDIGEEKPRQLVAGMASYYTPEELVGKSVIVLANLKPAKLCGVESNGMMLAADDGGAIVAALMPDKEIKSGSRIR

Sequences:

>Translated_715_residues
MEIRMSKSSSKPVLVTCGLPYANGKAHIGHLRTYVPADIYARSLKKEGREVTFVCGSDTHGTPIVVNAEELGITPTELVE
IYHKHFDETFKQLGIYFDAFGTTDDPENHNRTHDIVSRLIEKGYVYPKIIEIAYCPACNRFLPDRYVEGACPHCGETARG
DECDQGCGKHLEPGELQNPVCTICGGPAEYRQQEHFFFKLSEFSNYLMDYLSKDLGGTTNAINYARGWVKQGLTDWCITR
NLEWGVKFPGHEDLVVYVWVDAPIGYIAFTEEWAAQAGDSWEKFWKGDGEIVHFIGGDITYHHCIFWPAMLKGADYSVPT
AVVASGMVKIEDRKFSKTRGYVVWVGEDYLDHGFHPDLLRYYLASYTSHTKELNFSWRVLQEKINAELVAVLGNFLYRTM
LFAFKNYGEVPEGEVEPEIRKEIEKALVEVKAAMEEYEFKKAVDSAMALASFGNTYFQSHEPWKLIKEDRDACGQVVYNC
LHLAKALSLIFEPVIPQTMEKAWKGLGHESDIHAALYEETLVPLKAGTKLAKPEILFTKLEDDRIGEMEEIANQRVAAAN
AKRNGVKGGEKEPSKSEGMGPSEASKASEKTVDVATAEEKVQVETLPVIDYEDFAKLDIRVGKVLLVEPVKKSKKLLRIE
VDIGEEKPRQLVAGMASYYTPEELVGKSVIVLANLKPAKLCGVESNGMMLAADDGGAIVAALMPDKEIKSGSRIR
>Mature_715_residues
MEIRMSKSSSKPVLVTCGLPYANGKAHIGHLRTYVPADIYARSLKKEGREVTFVCGSDTHGTPIVVNAEELGITPTELVE
IYHKHFDETFKQLGIYFDAFGTTDDPENHNRTHDIVSRLIEKGYVYPKIIEIAYCPACNRFLPDRYVEGACPHCGETARG
DECDQGCGKHLEPGELQNPVCTICGGPAEYRQQEHFFFKLSEFSNYLMDYLSKDLGGTTNAINYARGWVKQGLTDWCITR
NLEWGVKFPGHEDLVVYVWVDAPIGYIAFTEEWAAQAGDSWEKFWKGDGEIVHFIGGDITYHHCIFWPAMLKGADYSVPT
AVVASGMVKIEDRKFSKTRGYVVWVGEDYLDHGFHPDLLRYYLASYTSHTKELNFSWRVLQEKINAELVAVLGNFLYRTM
LFAFKNYGEVPEGEVEPEIRKEIEKALVEVKAAMEEYEFKKAVDSAMALASFGNTYFQSHEPWKLIKEDRDACGQVVYNC
LHLAKALSLIFEPVIPQTMEKAWKGLGHESDIHAALYEETLVPLKAGTKLAKPEILFTKLEDDRIGEMEEIANQRVAAAN
AKRNGVKGGEKEPSKSEGMGPSEASKASEKTVDVATAEEKVQVETLPVIDYEDFAKLDIRVGKVLLVEPVKKSKKLLRIE
VDIGEEKPRQLVAGMASYYTPEELVGKSVIVLANLKPAKLCGVESNGMMLAADDGGAIVAALMPDKEIKSGSRIR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=568, Percent_Identity=34.5070422535211, Blast_Score=324, Evalue=2e-88,
Organism=Homo sapiens, GI24308436, Length=522, Percent_Identity=25.6704980842912, Blast_Score=153, Evalue=6e-37,
Organism=Homo sapiens, GI4507947, Length=82, Percent_Identity=45.1219512195122, Blast_Score=74, Evalue=7e-13,
Organism=Escherichia coli, GI1788432, Length=720, Percent_Identity=33.0555555555556, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789455, Length=110, Percent_Identity=39.0909090909091, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540794, Length=623, Percent_Identity=30.1765650080257, Blast_Score=304, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI212645096, Length=519, Percent_Identity=25.626204238921, Blast_Score=167, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71992781, Length=377, Percent_Identity=25.9946949602122, Blast_Score=128, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321704, Length=568, Percent_Identity=31.1619718309859, Blast_Score=276, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=23.5514018691589, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24655311, Length=561, Percent_Identity=32.620320855615, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI21358483, Length=515, Percent_Identity=23.495145631068, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21357223, Length=100, Percent_Identity=36, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_METMA (Q8PYJ4)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632891.2
- ProteinModelPortal:   Q8PYJ4
- SMR:   Q8PYJ4
- GeneID:   1479209
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0867
- NMPDR:   fig|192952.1.peg.867
- HOGENOM:   HBG721667
- OMA:   WGIWIDE
- ProtClustDB:   PRK00133
- BioCyc:   MMAZ192952:MM0867-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 79965; Mature: 79965

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 337-337

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRMSKSSSKPVLVTCGLPYANGKAHIGHLRTYVPADIYARSLKKEGREVTFVCGSDTH
CCEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCC
GTPIVVNAEELGITPTELVEIYHKHFDETFKQLGIYFDAFGTTDDPENHNRTHDIVSRLI
CCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHH
EKGYVYPKIIEIAYCPACNRFLPDRYVEGACPHCGETARGDECDQGCGKHLEPGELQNPV
HCCCCCHHHEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCE
CTICGGPAEYRQQEHFFFKLSEFSNYLMDYLSKDLGGTTNAINYARGWVKQGLTDWCITR
EEEECCHHHHCCCHHHEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHC
NLEWGVKFPGHEDLVVYVWVDAPIGYIAFTEEWAAQAGDSWEKFWKGDGEIVHFIGGDIT
CCCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHHCCCCHHHHHCCCCCEEEEECCCEE
YHHCIFWPAMLKGADYSVPTAVVASGMVKIEDRKFSKTRGYVVWVGEDYLDHGFHPDLLR
EHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCCCCCCCCEEEEECCHHHHCCCCHHHHH
YYLASYTSHTKELNFSWRVLQEKINAELVAVLGNFLYRTMLFAFKNYGEVPEGEVEPEIR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
KEIEKALVEVKAAMEEYEFKKAVDSAMALASFGNTYFQSHEPWKLIKEDRDACGQVVYNC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHH
LHLAKALSLIFEPVIPQTMEKAWKGLGHESDIHAALYEETLVPLKAGTKLAKPEILFTKL
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCEEEEEC
EDDRIGEMEEIANQRVAAANAKRNGVKGGEKEPSKSEGMGPSEASKASEKTVDVATAEEK
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHCEEEEEECCCC
VQVETLPVIDYEDFAKLDIRVGKVLLVEPVKKSKKLLRIEVDIGEEKPRQLVAGMASYYT
EEEEEEEEECCHHHHHHHEEECCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCC
PEELVGKSVIVLANLKPAKLCGVESNGMMLAADDGGAIVAALMPDKEIKSGSRIR
CHHHCCCEEEEEECCCCHHEECCCCCCEEEEECCCCEEEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MEIRMSKSSSKPVLVTCGLPYANGKAHIGHLRTYVPADIYARSLKKEGREVTFVCGSDTH
CCEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCEEEEEECCCCC
GTPIVVNAEELGITPTELVEIYHKHFDETFKQLGIYFDAFGTTDDPENHNRTHDIVSRLI
CCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHH
EKGYVYPKIIEIAYCPACNRFLPDRYVEGACPHCGETARGDECDQGCGKHLEPGELQNPV
HCCCCCHHHEEEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCE
CTICGGPAEYRQQEHFFFKLSEFSNYLMDYLSKDLGGTTNAINYARGWVKQGLTDWCITR
EEEECCHHHHCCCHHHEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHC
NLEWGVKFPGHEDLVVYVWVDAPIGYIAFTEEWAAQAGDSWEKFWKGDGEIVHFIGGDIT
CCCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHHCCCCHHHHHCCCCCEEEEECCCEE
YHHCIFWPAMLKGADYSVPTAVVASGMVKIEDRKFSKTRGYVVWVGEDYLDHGFHPDLLR
EHHHHHHHHHHCCCCCCCCHHHHHCCCEEEECCCCCCCCCEEEEECCHHHHCCCCHHHHH
YYLASYTSHTKELNFSWRVLQEKINAELVAVLGNFLYRTMLFAFKNYGEVPEGEVEPEIR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
KEIEKALVEVKAAMEEYEFKKAVDSAMALASFGNTYFQSHEPWKLIKEDRDACGQVVYNC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHH
LHLAKALSLIFEPVIPQTMEKAWKGLGHESDIHAALYEETLVPLKAGTKLAKPEILFTKL
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCEEEEEC
EDDRIGEMEEIANQRVAAANAKRNGVKGGEKEPSKSEGMGPSEASKASEKTVDVATAEEK
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHCEEEEEECCCC
VQVETLPVIDYEDFAKLDIRVGKVLLVEPVKKSKKLLRIEVDIGEEKPRQLVAGMASYYT
EEEEEEEEECCHHHHHHHEEECCEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCC
PEELVGKSVIVLANLKPAKLCGVESNGMMLAADDGGAIVAALMPDKEIKSGSRIR
CHHHCCCEEEEEECCCCHHEECCCCCCEEEEECCCCEEEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824