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The map label for this gene is tgt

Identifier: 161349969

GI number: 161349969

Start: 579286

End: 580416

Strand: Reverse

Name: tgt

Synonym: ACIAD0590

Alternate gene names: 161349969

Gene position: 580416-579286 (Counterclockwise)

Preceding gene: 50083827

Following gene: 50083825

Centisome position: 16.13

GC content: 46.6

Gene sequence:

>1131_bases
ATGAAGTTTGAAAAATTAGGTCAATCTGGCCGCGCCCGCCGTGGTCGTTTAACCCTTGCACACGGTGTGGTCGAAACACC
TGTGTTTATGCCCGTTGGCACATACGGCACTGTAAAAGGTATGTTACCGCGCGATATCGAAGAAATTCAAGCGCAAATTA
TTTTAGGCAACACCTTTCACCTGTACTTGCGTCCGGGCCTTGAGGTGATTAAAGAGCATGGTGGATTACATGAATTCATC
AAGTGGGATAAACCGATTCTGACCGATTCAGGCGGCTTTCAGGTGTTTAGCCTTGGCGCGATGCGTAAAATCAAAGAAGA
AGGTGTGACCTTCCGCTCACCGATTGACGGTTCAAAAGTGTTTTTGTCACCTGAGATTTCTATGGAAATCCAGCAAGTGC
TCAATTCCGACATCGTGATGATTTTTGACGAATGCACGCCCTACCCAGCCACTCACGAAGAAGCGCAAAAATCGTTGCAG
TTGTCTTTGCGCTGGGCCAAACGTTGTAAAACCCATCATCATGAGCAACTCAATAATACCAATGCGCTATTTGGAATTAT
TCAGGGCGGTATGTATGAAGATCTTCGTGATGAATCCCTCAATGGTTTGCTCGAAATTGGATTTGATGGCTATGCCATCG
GTGGGTTATCGGTAGGCGAACCGAAAGAAGAAATGATCAAGGTACTCGATTATCTGCCGGCTAAAATGCCTGAAGATAAG
CCGCGTTACCTGATGGGTGTGGGTAAACCAGAAGATATCGTCGAAGCGGTGCGCCGTGGTGTCGATATGTTTGACTGCGT
GATGCCGACCCGTAATGCACGTAACGGTCATTACTTTGTGACAGACGGTTTAGTACGTATCCGCAACAGTAAATATCGTC
ATGATCAAAGCCCGCTCGATCCACATTGCGATTGCTATACTTGCAAAAACTTTACCCGTGCCTACCTTTACCATCTTGAA
AAATGTGGTGAAATGTTGGCATCAATGCTCGGTACAATTCACAACTTACGGTATTATCAACGCCTGACTCAAGGCATTCG
TGATGCACTTGAGCAGGGTACCTTTGATGAGTTTGTCACCGACTTCTACACGCGTCGTGGTTTAGATGTACCACCCGCAC
CGGTGGATTAA

Upstream 100 bases:

>100_bases
TACATTTAGTAGTAGCGCTACACGTCCAAATCAAGGAAAATAGCCGCCTTTAAATAGGTTAATCAGCGAACTGTTTTTTC
GCCTTGATTTGGAAAGTTGC

Downstream 100 bases:

>100_bases
TTCATCAGAATTTGCGCTCAACAAAAAGACAAGGTAAATTGCAGAGCGAACTGGTATTTTTGTCATGATTTGACGCCCAT
TTTTTAACTTTAGGAAACCA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKFEKLGQSGRARRGRLTLAHGVVETPVFMPVGTYGTVKGMLPRDIEEIQAQIILGNTFHLYLRPGLEVIKEHGGLHEFI
KWDKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKVFLSPEISMEIQQVLNSDIVMIFDECTPYPATHEEAQKSLQ
LSLRWAKRCKTHHHEQLNNTNALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPAKMPEDK
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQSPLDPHCDCYTCKNFTRAYLYHLE
KCGEMLASMLGTIHNLRYYQRLTQGIRDALEQGTFDEFVTDFYTRRGLDVPPAPVD

Sequences:

>Translated_376_residues
MKFEKLGQSGRARRGRLTLAHGVVETPVFMPVGTYGTVKGMLPRDIEEIQAQIILGNTFHLYLRPGLEVIKEHGGLHEFI
KWDKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKVFLSPEISMEIQQVLNSDIVMIFDECTPYPATHEEAQKSLQ
LSLRWAKRCKTHHHEQLNNTNALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPAKMPEDK
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQSPLDPHCDCYTCKNFTRAYLYHLE
KCGEMLASMLGTIHNLRYYQRLTQGIRDALEQGTFDEFVTDFYTRRGLDVPPAPVD
>Mature_376_residues
MKFEKLGQSGRARRGRLTLAHGVVETPVFMPVGTYGTVKGMLPRDIEEIQAQIILGNTFHLYLRPGLEVIKEHGGLHEFI
KWDKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKVFLSPEISMEIQQVLNSDIVMIFDECTPYPATHEEAQKSLQ
LSLRWAKRCKTHHHEQLNNTNALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPAKMPEDK
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQSPLDPHCDCYTCKNFTRAYLYHLE
KCGEMLASMLGTIHNLRYYQRLTQGIRDALEQGTFDEFVTDFYTRRGLDVPPAPVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=352, Percent_Identity=44.0340909090909, Blast_Score=309, Evalue=3e-84,
Organism=Escherichia coli, GI1786607, Length=376, Percent_Identity=67.0212765957447, Blast_Score=542, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17544680, Length=363, Percent_Identity=41.8732782369146, Blast_Score=297, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI25146502, Length=247, Percent_Identity=26.3157894736842, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19920510, Length=356, Percent_Identity=45.2247191011236, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI21355311, Length=257, Percent_Identity=26.0700389105058, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_ACIAD (Q6FEJ4)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045336.2
- ProteinModelPortal:   Q6FEJ4
- SMR:   Q6FEJ4
- STRING:   Q6FEJ4
- GeneID:   2881193
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0590
- NMPDR:   fig|62977.3.peg.128
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q6FEJ4
- ProtClustDB:   PRK00112
- BioCyc:   ASP62977:ACIAD0590-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42710; Mature: 42710

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: ACT_SITE 99-99 BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFEKLGQSGRARRGRLTLAHGVVETPVFMPVGTYGTVKGMLPRDIEEIQAQIILGNTFH
CCHHHHCCCCCCCCCCEEEECCHHCCCCEEECCCCCCCCCCCCCCHHHHHHEEEECCEEE
LYLRPGLEVIKEHGGLHEFIKWDKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKV
EEECCHHHHHHHCCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECCCCCCEE
FLSPEISMEIQQVLNSDIVMIFDECTPYPATHEEAQKSLQLSLRWAKRCKTHHHEQLNNT
EECCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPAKMPEDK
CHHHHHHCCCHHHHHHHHHCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCC
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQSPLD
CCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCC
PHCDCYTCKNFTRAYLYHLEKCGEMLASMLGTIHNLRYYQRLTQGIRDALEQGTFDEFVT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
DFYTRRGLDVPPAPVD
HHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKFEKLGQSGRARRGRLTLAHGVVETPVFMPVGTYGTVKGMLPRDIEEIQAQIILGNTFH
CCHHHHCCCCCCCCCCEEEECCHHCCCCEEECCCCCCCCCCCCCCHHHHHHEEEECCEEE
LYLRPGLEVIKEHGGLHEFIKWDKPILTDSGGFQVFSLGAMRKIKEEGVTFRSPIDGSKV
EEECCHHHHHHHCCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECCCCCCEE
FLSPEISMEIQQVLNSDIVMIFDECTPYPATHEEAQKSLQLSLRWAKRCKTHHHEQLNNT
EECCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NALFGIIQGGMYEDLRDESLNGLLEIGFDGYAIGGLSVGEPKEEMIKVLDYLPAKMPEDK
CHHHHHHCCCHHHHHHHHHCCCEEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCC
PRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYFVTDGLVRIRNSKYRHDQSPLD
CCEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCC
PHCDCYTCKNFTRAYLYHLEKCGEMLASMLGTIHNLRYYQRLTQGIRDALEQGTFDEFVT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
DFYTRRGLDVPPAPVD
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA