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The map label for this gene is queA

Identifier: 50083827

GI number: 50083827

Start: 580735

End: 581769

Strand: Reverse

Name: queA

Synonym: ACIAD0591

Alternate gene names: 50083827

Gene position: 581769-580735 (Counterclockwise)

Preceding gene: 50083838

Following gene: 161349969

Centisome position: 16.17

GC content: 42.32

Gene sequence:

>1035_bases
ATGCAACTTTCCGACTTTAATTTTGATCTGCCTGATGAGCTGATTGCTCGCTATCCACTCGACACACGTTCTGCTTCTCG
CTTACTGCATTTAGATGCGCATGGTCATTATCACGATCATGCTTTTACCGATATTCTAGATTTGATCAATGACGGCGATT
TGCTGGTGCTTAATGACACCAAAGTAATGAAAGCACGGCTCAAAGGAAAACGCGCTTCAGGTGGTGCCATTGAAATTTTA
GTCGAGCGGATGTTGGACAATCAGATTGCACATTGTCATATTAAGGCCAGTAATACTCCCAAAGCAGGTGCAGAATTATT
TGTAGGGGAAAACGGCATTAAAGTTACGGTTCAGGGCCGTCATGAAAATTTGTTTGTAGTGGAGTTTTCGGAACCCATTT
TATCGGTATTGGATCGTTATGGCCAACTGCCAATTCCACCTTATTTTAATCGTGAAGCAGAAGAAATTGATACCGAACGT
TATCAAACTGTTTTTCACGATCCTCAAAAAATTGCCAGTGTTGCTGCTCCCACAGCCAGCCTGCATTTTGATCAGGATTT
ACTAGAAAAACTCGCAGCTAAAAATATCCAAAAAACCTTTGTCACGCTACATGTGGGGGCAGGTACATTCATGCCTGTAA
GAACAGATGACATTAAAAACCATATTATGCACAGCGAATGGTGTGATGTACCAGAATCGACCGCCGAGCTGATCCGTCAG
ACCAAGGCACGTGGCAATAAAGTGATTGCTGTAGGTACAACAGCTACACGTGCACTTGAAAGTGCCGCACAAGCAAATGG
TGGTAAAATTGATGCATGGACTGGTGATACTCAAATTTTTATTTATCCAGGTTATCAGTTCTGTGTAGTTGATCGTTTGA
TTACCAACTTCCATTTACCAGAATCCACCCTGCTTATGCTAGTTTCGGCGCTCTCTAATCGTAACAATATCTTGTCTGCT
TACGAACATGCAGTTCAATCAAAATATCGGTTCTTTAGTTACGGCGATGCGATGCTGATTGATCAAGCGGTCTAA

Upstream 100 bases:

>100_bases
CACTTTTAATACAAACAATAAGTTTCAATAAAAAAAGGTCGCTGCCCAATCACTTTGAAAAGCACTATACTATCGCCAAG
TTTTGGCCTGTACTTGAGTT

Downstream 100 bases:

>100_bases
ATTATGAGGCTTGCCTCTTTAATTATAGTGTCGTTGTTTTTGTAGGAAACACAGCCCGCTAGTTTGTGAAAATCTGATGT
CTTTTAGTCAGATCGGATTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MQLSDFNFDLPDELIARYPLDTRSASRLLHLDAHGHYHDHAFTDILDLINDGDLLVLNDTKVMKARLKGKRASGGAIEIL
VERMLDNQIAHCHIKASNTPKAGAELFVGENGIKVTVQGRHENLFVVEFSEPILSVLDRYGQLPIPPYFNREAEEIDTER
YQTVFHDPQKIASVAAPTASLHFDQDLLEKLAAKNIQKTFVTLHVGAGTFMPVRTDDIKNHIMHSEWCDVPESTAELIRQ
TKARGNKVIAVGTTATRALESAAQANGGKIDAWTGDTQIFIYPGYQFCVVDRLITNFHLPESTLLMLVSALSNRNNILSA
YEHAVQSKYRFFSYGDAMLIDQAV

Sequences:

>Translated_344_residues
MQLSDFNFDLPDELIARYPLDTRSASRLLHLDAHGHYHDHAFTDILDLINDGDLLVLNDTKVMKARLKGKRASGGAIEIL
VERMLDNQIAHCHIKASNTPKAGAELFVGENGIKVTVQGRHENLFVVEFSEPILSVLDRYGQLPIPPYFNREAEEIDTER
YQTVFHDPQKIASVAAPTASLHFDQDLLEKLAAKNIQKTFVTLHVGAGTFMPVRTDDIKNHIMHSEWCDVPESTAELIRQ
TKARGNKVIAVGTTATRALESAAQANGGKIDAWTGDTQIFIYPGYQFCVVDRLITNFHLPESTLLMLVSALSNRNNILSA
YEHAVQSKYRFFSYGDAMLIDQAV
>Mature_344_residues
MQLSDFNFDLPDELIARYPLDTRSASRLLHLDAHGHYHDHAFTDILDLINDGDLLVLNDTKVMKARLKGKRASGGAIEIL
VERMLDNQIAHCHIKASNTPKAGAELFVGENGIKVTVQGRHENLFVVEFSEPILSVLDRYGQLPIPPYFNREAEEIDTER
YQTVFHDPQKIASVAAPTASLHFDQDLLEKLAAKNIQKTFVTLHVGAGTFMPVRTDDIKNHIMHSEWCDVPESTAELIRQ
TKARGNKVIAVGTTATRALESAAQANGGKIDAWTGDTQIFIYPGYQFCVVDRLITNFHLPESTLLMLVSALSNRNNILSA
YEHAVQSKYRFFSYGDAMLIDQAV

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=55.9420289855072, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_ACIAD (Q6FEJ3)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_045337.1
- ProteinModelPortal:   Q6FEJ3
- SMR:   Q6FEJ3
- STRING:   Q6FEJ3
- GeneID:   2879573
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD0591
- NMPDR:   fig|62977.3.peg.129
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q6FEJ3
- ProtClustDB:   PRK00147
- BioCyc:   ASP62977:ACIAD0591-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38445; Mature: 38445

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSDFNFDLPDELIARYPLDTRSASRLLHLDAHGHYHDHAFTDILDLINDGDLLVLNDT
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCH
KVMKARLKGKRASGGAIEILVERMLDNQIAHCHIKASNTPKAGAELFVGENGIKVTVQGR
HHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEECCCCEEEEEEEC
HENLFVVEFSEPILSVLDRYGQLPIPPYFNREAEEIDTERYQTVFHDPQKIASVAAPTAS
CCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHH
LHFDQDLLEKLAAKNIQKTFVTLHVGAGTFMPVRTDDIKNHIMHSEWCDVPESTAELIRQ
CCCCHHHHHHHHHCCCEEEEEEEEECCCEEEEEECHHHHHHHCCHHHCCCCHHHHHHHHH
TKARGNKVIAVGTTATRALESAAQANGGKIDAWTGDTQIFIYPGYQFCVVDRLITNFHLP
HHHCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHCCCC
ESTLLMLVSALSNRNNILSAYEHAVQSKYRFFSYGDAMLIDQAV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCEEEEECCC
>Mature Secondary Structure
MQLSDFNFDLPDELIARYPLDTRSASRLLHLDAHGHYHDHAFTDILDLINDGDLLVLNDT
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCH
KVMKARLKGKRASGGAIEILVERMLDNQIAHCHIKASNTPKAGAELFVGENGIKVTVQGR
HHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEECCCCEEEEEEEC
HENLFVVEFSEPILSVLDRYGQLPIPPYFNREAEEIDTERYQTVFHDPQKIASVAAPTAS
CCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHH
LHFDQDLLEKLAAKNIQKTFVTLHVGAGTFMPVRTDDIKNHIMHSEWCDVPESTAELIRQ
CCCCHHHHHHHHHCCCEEEEEEEEECCCEEEEEECHHHHHHHCCHHHCCCCHHHHHHHHH
TKARGNKVIAVGTTATRALESAAQANGGKIDAWTGDTQIFIYPGYQFCVVDRLITNFHLP
HHHCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHCCCC
ESTLLMLVSALSNRNNILSAYEHAVQSKYRFFSYGDAMLIDQAV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA