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The map label for this gene is nth [H]

Identifier: 16127961

GI number: 16127961

Start: 3987130

End: 3987855

Strand: Direct

Name: nth [H]

Synonym: CC_3731

Alternate gene names: 16127961

Gene position: 3987130-3987855 (Clockwise)

Preceding gene: 16127958

Following gene: 16127963

Centisome position: 99.26

GC content: 65.84

Gene sequence:

>726_bases
ATGGTCGAGACTCCCCGTAAAGCCGCTTTGGTCAGCAAGACGCCGGTCCGCAAGGCCGCCAGGAAGCCCGTCAAACGCCT
CTCGCCGGCCCAGCGCGAGCGCGTCGCTGTCCTGTTCGACCGGTTCGAGGGCTTGGACCTGCATCCCAAGACGGAGCTCA
ACTATTCCAACGCCTATGAGCTGGTCACGGCCGTCGCCCTGTCGGCCCAGGCCACCGACGTCCAGGTCAACAAGGCCACC
GGCCCGCTGTTCCAGGTCGCCAACAGCGCCGAGGCGATGCTGGCGCTCGGGGAAGAGGGGCTGACCAAGTACATCGCCTC
GATCGGCCTGTTCCGCAGCAAGGCCAAGAATGTCATCGCCGCCGCCCACATCATCATGAACCAGCATGGGGGCGAGGTTC
CGCTCAACCGCGAAGACCTGGAAGCCCTGCCCGGCGTTGGCCGCAAGACCGCCAGCGTGGTGTTGAACGAGCTGGATATC
GAGCCGGCCATCGCGGTCGACACCCACGTGTTCCGGGTCTCGCATCGCCTGAAGCTGTCGAGCGGCAAGACACCGGACGC
GGTCGAGCAGGACCTGATGCGGGTGGTCCCGCCGCCCTACCAGACGCGCGCCCACCACTGGCTGATCCTGCACGGCCGCT
ATGTGTGCGTGGCGCGAAAGCCCAAATGCGAGATCTGCAAGATCAGCGACCTGTGTCCATCGCGGGAGCTCTTCTTGGGG
GCGTGA

Upstream 100 bases:

>100_bases
GGGCGATCACCCGGTCCATGTAGATCGGCTTGGCCATGGCGCGAATTTAGGTGTCAGCCGCGCTCTTGGCGAGCGGAGTC
CCCCACGGCATGGTCGCCCC

Downstream 100 bases:

>100_bases
GCCCAAAAATCCTCTCCCTGTGGGAGAGGTGTCGGCGCAGCCGACGGAGAGGGGAGAGGCCGCGCCAAGCGACTTCCCCC
TCCGGCGCTTCGCGCCACCT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MVETPRKAALVSKTPVRKAARKPVKRLSPAQRERVAVLFDRFEGLDLHPKTELNYSNAYELVTAVALSAQATDVQVNKAT
GPLFQVANSAEAMLALGEEGLTKYIASIGLFRSKAKNVIAAAHIIMNQHGGEVPLNREDLEALPGVGRKTASVVLNELDI
EPAIAVDTHVFRVSHRLKLSSGKTPDAVEQDLMRVVPPPYQTRAHHWLILHGRYVCVARKPKCEICKISDLCPSRELFLG
A

Sequences:

>Translated_241_residues
MVETPRKAALVSKTPVRKAARKPVKRLSPAQRERVAVLFDRFEGLDLHPKTELNYSNAYELVTAVALSAQATDVQVNKAT
GPLFQVANSAEAMLALGEEGLTKYIASIGLFRSKAKNVIAAAHIIMNQHGGEVPLNREDLEALPGVGRKTASVVLNELDI
EPAIAVDTHVFRVSHRLKLSSGKTPDAVEQDLMRVVPPPYQTRAHHWLILHGRYVCVARKPKCEICKISDLCPSRELFLG
A
>Mature_241_residues
MVETPRKAALVSKTPVRKAARKPVKRLSPAQRERVAVLFDRFEGLDLHPKTELNYSNAYELVTAVALSAQATDVQVNKAT
GPLFQVANSAEAMLALGEEGLTKYIASIGLFRSKAKNVIAAAHIIMNQHGGEVPLNREDLEALPGVGRKTASVVLNELDI
EPAIAVDTHVFRVSHRLKLSSGKTPDAVEQDLMRVVPPPYQTRAHHWLILHGRYVCVARKPKCEICKISDLCPSRELFLG
A

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=29.0502793296089, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI1787920, Length=199, Percent_Identity=55.7788944723618, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=30.5084745762712, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550361, Length=182, Percent_Identity=28.021978021978, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26455; Mature: 26455

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVETPRKAALVSKTPVRKAARKPVKRLSPAQRERVAVLFDRFEGLDLHPKTELNYSNAYE
CCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LVTAVALSAQATDVQVNKATGPLFQVANSAEAMLALGEEGLTKYIASIGLFRSKAKNVIA
HHHHHHHHCCCCEEEEECCCCHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AAHIIMNQHGGEVPLNREDLEALPGVGRKTASVVLNELDIEPAIAVDTHVFRVSHRLKLS
HHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEHHHHHEEHHHEEEEC
SGKTPDAVEQDLMRVVPPPYQTRAHHWLILHGRYVCVARKPKCEICKISDLCPSRELFLG
CCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEECCCCCCEEECCCCCCCCHHCCC
A
C
>Mature Secondary Structure
MVETPRKAALVSKTPVRKAARKPVKRLSPAQRERVAVLFDRFEGLDLHPKTELNYSNAYE
CCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LVTAVALSAQATDVQVNKATGPLFQVANSAEAMLALGEEGLTKYIASIGLFRSKAKNVIA
HHHHHHHHCCCCEEEEECCCCHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AAHIIMNQHGGEVPLNREDLEALPGVGRKTASVVLNELDIEPAIAVDTHVFRVSHRLKLS
HHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEHHHHHEEHHHEEEEC
SGKTPDAVEQDLMRVVPPPYQTRAHHWLILHGRYVCVARKPKCEICKISDLCPSRELFLG
CCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEECCCCCCEEECCCCCCCCHHCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]