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The map label for this gene is emrA [H]

Identifier: 16127944

GI number: 16127944

Start: 3968563

End: 3969753

Strand: Direct

Name: emrA [H]

Synonym: CC_3714

Alternate gene names: 16127944

Gene position: 3968563-3969753 (Clockwise)

Preceding gene: 16127943

Following gene: 16127945

Centisome position: 98.8

GC content: 68.35

Gene sequence:

>1191_bases
TTGGACGACTGCGTCCATTTATCCCTTGTATGTTGCGCCGGACCTGACTATCCGGCGCGCTTGTTGTCATTCGACAAGGG
CTTGGCCATGGCGGCGTCAGAGAAGAAGAAGCTGGTTCCCCTGATCGTCGGTGGCGTGGCGATCGCTGCGGTTCTGGTCG
GCGGAACCCTGTGGTTCCTTGACAAGCAGCACTTCGAGAGCACGGACAACGCCTTCGTCCAGGCCGACACTGTGCAGGTC
AGCCCGCAAGTCTCCGGCTATGTCGCCGAGGTGCTTGTCGCGGATAACCAACGCGTCGAGCCCGGTCAGGTGCTGGCCAA
GATCGATCCGTCGACCTTCCAGGCGCGGGTGGACCAGGCGATCGCCAACGCCGCGGCGGCGGACGCCGCCGTTAGGGCGA
TCGATGACAAGAACAGCCTGGAGCAGGCCGTGATCGCCCAGAGGGCGGCCGGCGTGACCAGCGCCCAGGCCGACGCCAGC
CGCGCCAAGGCCGACCTTGACCGCTACAACGCCCTGGCCGACCAGGGCTGGGTGTCGCAGCAGAAGGTGCAGACCACACG
CGCGGCCGCGACCCAGTCCGCCGCCGGCGTCGCCAGCGCCCAGGCCGCGCTGGAAGCCGAGAAGCGCGCCGCCCAATCGC
TGGGCTCGGCCAAGGCCCAGGCAATCGCCCAGGCCGCCGCCGCCCATGCCGCTGTCGAACAGGCCAAGCTGGACCTTGAA
CGCACCGTGATCCGCGCGCCGGTCAGCGGTGTGGTCGGCGCCCGCGCCGTCCGCCCGGGCCAACTGGTTCAGCCGGGCGT
CGCCCTGATGTCGGTGGTGCCGCTGGGCCAGGCCTATGTCGTCGCCAACTTCAAGGAGACCCAGGTCGCCCGCCTGCGCA
TCGGCCAGCCCGTCGAAATCAAGGCAGACGCCTTCGGCAAAGAAAAGATCGTCGGCAAGGTCGACAGCTTCGCACCCGCT
ACGGGCCAAGAGTTCGCGCTGATCCCGATTGAGAACGCCGTCGGCAACTTCACCAAGATCACCCAGCGCCTCCCGGTGAA
GATCGCCGTCGACCGCAGCCAGCTGGGCGCAGCCCTGCGGCCCGGTCTGTCGGTCGAGATCAAGGTGGATGTCCGCGATC
GCTCGGGCCCCTCGTTCGCAGAGGCGGCGCAAGTGACGCCGCAGGTGGCCCGACAGGGCGCGGCCCGCTAG

Upstream 100 bases:

>100_bases
CAATGCAAGCGGAAATTGCGGCGCGGTGTCGCAGCCCCATGATTCGCCCCCTCCGTCCTGCGGCGGATAGAGGCGTTTGA
TCGAGCGCCTCTGTTGATTT

Downstream 100 bases:

>100_bases
TCGGCCCGCCTGTCATGGCCGACGCCGCCCTCCCCGCCGATAGCCCCAGCGCGCCAGCAACGCCCGGCGCGACAGCGCCG
GTCGATTGGACAAAGCTGTT

Product: HlyD family secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MDDCVHLSLVCCAGPDYPARLLSFDKGLAMAASEKKKLVPLIVGGVAIAAVLVGGTLWFLDKQHFESTDNAFVQADTVQV
SPQVSGYVAEVLVADNQRVEPGQVLAKIDPSTFQARVDQAIANAAAADAAVRAIDDKNSLEQAVIAQRAAGVTSAQADAS
RAKADLDRYNALADQGWVSQQKVQTTRAAATQSAAGVASAQAALEAEKRAAQSLGSAKAQAIAQAAAAHAAVEQAKLDLE
RTVIRAPVSGVVGARAVRPGQLVQPGVALMSVVPLGQAYVVANFKETQVARLRIGQPVEIKADAFGKEKIVGKVDSFAPA
TGQEFALIPIENAVGNFTKITQRLPVKIAVDRSQLGAALRPGLSVEIKVDVRDRSGPSFAEAAQVTPQVARQGAAR

Sequences:

>Translated_396_residues
MDDCVHLSLVCCAGPDYPARLLSFDKGLAMAASEKKKLVPLIVGGVAIAAVLVGGTLWFLDKQHFESTDNAFVQADTVQV
SPQVSGYVAEVLVADNQRVEPGQVLAKIDPSTFQARVDQAIANAAAADAAVRAIDDKNSLEQAVIAQRAAGVTSAQADAS
RAKADLDRYNALADQGWVSQQKVQTTRAAATQSAAGVASAQAALEAEKRAAQSLGSAKAQAIAQAAAAHAAVEQAKLDLE
RTVIRAPVSGVVGARAVRPGQLVQPGVALMSVVPLGQAYVVANFKETQVARLRIGQPVEIKADAFGKEKIVGKVDSFAPA
TGQEFALIPIENAVGNFTKITQRLPVKIAVDRSQLGAALRPGLSVEIKVDVRDRSGPSFAEAAQVTPQVARQGAAR
>Mature_396_residues
MDDCVHLSLVCCAGPDYPARLLSFDKGLAMAASEKKKLVPLIVGGVAIAAVLVGGTLWFLDKQHFESTDNAFVQADTVQV
SPQVSGYVAEVLVADNQRVEPGQVLAKIDPSTFQARVDQAIANAAAADAAVRAIDDKNSLEQAVIAQRAAGVTSAQADAS
RAKADLDRYNALADQGWVSQQKVQTTRAAATQSAAGVASAQAALEAEKRAAQSLGSAKAQAIAQAAAAHAAVEQAKLDLE
RTVIRAPVSGVVGARAVRPGQLVQPGVALMSVVPLGQAYVVANFKETQVARLRIGQPVEIKADAFGKEKIVGKVDSFAPA
TGQEFALIPIENAVGNFTKITQRLPVKIAVDRSQLGAALRPGLSVEIKVDVRDRSGPSFAEAAQVTPQVARQGAAR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=343, Percent_Identity=36.1516034985423, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788711, Length=328, Percent_Identity=33.5365853658537, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1790519, Length=311, Percent_Identity=31.8327974276527, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081951, Length=329, Percent_Identity=27.9635258358663, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790024, Length=289, Percent_Identity=28.0276816608997, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790012, Length=280, Percent_Identity=28.2142857142857, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789637, Length=295, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789900, Length=305, Percent_Identity=26.5573770491803, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41221; Mature: 41221

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDCVHLSLVCCAGPDYPARLLSFDKGLAMAASEKKKLVPLIVGGVAIAAVLVGGTLWFL
CCCCEEEEEEEECCCCCHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEEE
DKQHFESTDNAFVQADTVQVSPQVSGYVAEVLVADNQRVEPGQVLAKIDPSTFQARVDQA
EHHHCCCCCCCEEEEEEEEECCCHHHHHEEEEECCCCCCCCCCEEEEECCHHHHHHHHHH
IANAAAADAAVRAIDDKNSLEQAVIAQRAAGVTSAQADASRAKADLDRYNALADQGWVSQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
QKVQTTRAAATQSAAGVASAQAALEAEKRAAQSLGSAKAQAIAQAAAAHAAVEQAKLDLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTVIRAPVSGVVGARAVRPGQLVQPGVALMSVVPLGQAYVVANFKETQVARLRIGQPVEI
HHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCEE
KADAFGKEKIVGKVDSFAPATGQEFALIPIENAVGNFTKITQRLPVKIAVDRSQLGAALR
ECCCCCCHHHHHHHHCCCCCCCCCEEEEECHHHHCHHHHHHHHCCEEEEECHHHHCHHHC
PGLSVEIKVDVRDRSGPSFAEAAQVTPQVARQGAAR
CCCEEEEEEEEECCCCCCHHHHHHCCHHHHHCCCCC
>Mature Secondary Structure
MDDCVHLSLVCCAGPDYPARLLSFDKGLAMAASEKKKLVPLIVGGVAIAAVLVGGTLWFL
CCCCEEEEEEEECCCCCHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHEEE
DKQHFESTDNAFVQADTVQVSPQVSGYVAEVLVADNQRVEPGQVLAKIDPSTFQARVDQA
EHHHCCCCCCCEEEEEEEEECCCHHHHHEEEEECCCCCCCCCCEEEEECCHHHHHHHHHH
IANAAAADAAVRAIDDKNSLEQAVIAQRAAGVTSAQADASRAKADLDRYNALADQGWVSQ
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
QKVQTTRAAATQSAAGVASAQAALEAEKRAAQSLGSAKAQAIAQAAAAHAAVEQAKLDLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTVIRAPVSGVVGARAVRPGQLVQPGVALMSVVPLGQAYVVANFKETQVARLRIGQPVEI
HHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCEE
KADAFGKEKIVGKVDSFAPATGQEFALIPIENAVGNFTKITQRLPVKIAVDRSQLGAALR
ECCCCCCHHHHHHHHCCCCCCCCCEEEEECHHHHCHHHHHHHHCCEEEEECHHHHCHHHC
PGLSVEIKVDVRDRSGPSFAEAAQVTPQVARQGAAR
CCCEEEEEEEEECCCCCCHHHHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]