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The map label for this gene is emrB [H]

Identifier: 16127945

GI number: 16127945

Start: 3969768

End: 3971369

Strand: Direct

Name: emrB [H]

Synonym: CC_3715

Alternate gene names: 16127945

Gene position: 3969768-3971369 (Clockwise)

Preceding gene: 16127944

Following gene: 16127946

Centisome position: 98.83

GC content: 65.98

Gene sequence:

>1602_bases
ATGGCCGACGCCGCCCTCCCCGCCGATAGCCCCAGCGCGCCAGCAACGCCCGGCGCGACAGCGCCGGTCGATTGGACAAA
GCTGTTTCTGGGCTTTGGGGCCATGGTCATCGGCCAGTTCATGGCCATTCTCGACATTCAGATCGTGGCGGCGTCCCTGC
CGCAGATCCAGGCCGGGGTCGGGGCCAGCACCGACCAGGTCAGCTGGATCCAGACCGCCTATCTGATCCCCGAAGTCGTG
ATGATCCCGCTATCGGGATACCTCTCGCGCCTGTGGGGCACGCAGAAGCTCTATCTGGCGTCCTGCGCCGGCTTCATCGT
CATGAGCATCCTGACGGGGCTGTCGACCTCGATCGACATGATGATCATCACCCGGGCCCTGCAGGGCTTCATCGGCGGGG
CGATGATCCCGACGGTGTTCGCCGTCGCCTTCACGGCCTTTCCACCTGAGCGTCGGGTGACGGCCAGTGTGATCATGGGC
CTGATCGTCACCCTGGCCCCGACGGTCGGCCCGACCCTGGGCGGTCACCTGACCGAGTGGCTGAGCTGGCGATGGCTGTT
CTTCATCAACGTGCCGACCGGCCTGATCGTACTGTTCGGCGTGGCGCGCTGGGGCGACTTCGACAAGGGCGATCCCAGCC
TGGCCAAGGGCTTCGACTGGTTCGGCCTCGCGGTGATGGCCGTTTTCCTGATGAGCATGCAGTACGTTCTGGAGGAAGGC
GCCAAGGAGGACTGGTTATCCGACAGCCTGATCCTGTGGTTGACCGTCGTCGCGGTCTTCGGCGGGGTCGTCTTCGTGTG
GCGCCAACTGACCTATCGCAACCCGATCGTCGAGCTGCGCGCCTTCGCCAATCGCAATTTTACCGTCGGCGTCCTGATGA
CCGCGGTGTCCGGGGCCAGCCTGTTTGGCGGCACCTTCCTGCTGCCGCAGTTCCTGGGGCGCGTGCGACACTATTCGGCG
TCCGAGGTGGGCACGACCATGGTCATATCGGGCCTGTCGATGTTCCTGACCGGTCCGATCGCCGGGCGCCTCGTCCGCAA
GACCGATCCACGCCTGCCGATGTGCGTGGGCTTCCTGCTGGCCGGCTTTGGCATGTATATGGCCCACGGGGTGACCAAGG
ACTGGGGCTTCTGGGAGTTCGCCGGCGTTCAGGCTTGCCGGGGGGTCGGCGTGATGATCGCCATGATCGCCACCCAGCAG
ATCACGATGAGCACCCTGCCGCCTCACATGGTCAAGAACGCTTCGGGGCTCGTGAACCTGTCGCGTAACACCGGCGGCGC
GATCGGCCTCGCCTTGTTGGCCACCTCGATCACACAACAGACGGCGCTCTACTACGACGACCTGACCTCGAAGGTGACCC
AGGGCAGCGCTGCGGCCGGCATGATCGCGGGCCTCACCGAGCGCATGATGCAGCTGGGCGTCGCCGATCCGGCCGGCGCG
GCGCGCAAGGCCGTCGGCGGCATGATGCAGCAGCAGGCGACCGTGATGGCCTTCGGCGACAGTTTCACCCTGCTGGCGAT
CGGCTGCTTTATCGCGGCGGCTGTTTCCCTGTTGGCCGCGCCCGTCAAGAACGCGCCACCCCCGCCGTCTGACGCCCACT
GA

Upstream 100 bases:

>100_bases
TGGATGTCCGCGATCGCTCGGGCCCCTCGTTCGCAGAGGCGGCGCAAGTGACGCCGCAGGTGGCCCGACAGGGCGCGGCC
CGCTAGTCGGCCCGCCTGTC

Downstream 100 bases:

>100_bases
TGTCTGGAGTCTTCTGATGCCCCGCGTCCCAGGACAGATCGATGTCGCCAAGACCGAGGCGATCCTCGACGCGGCCGTCG
AGGTCATCGGCGAGCGCGGT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MADAALPADSPSAPATPGATAPVDWTKLFLGFGAMVIGQFMAILDIQIVAASLPQIQAGVGASTDQVSWIQTAYLIPEVV
MIPLSGYLSRLWGTQKLYLASCAGFIVMSILTGLSTSIDMMIITRALQGFIGGAMIPTVFAVAFTAFPPERRVTASVIMG
LIVTLAPTVGPTLGGHLTEWLSWRWLFFINVPTGLIVLFGVARWGDFDKGDPSLAKGFDWFGLAVMAVFLMSMQYVLEEG
AKEDWLSDSLILWLTVVAVFGGVVFVWRQLTYRNPIVELRAFANRNFTVGVLMTAVSGASLFGGTFLLPQFLGRVRHYSA
SEVGTTMVISGLSMFLTGPIAGRLVRKTDPRLPMCVGFLLAGFGMYMAHGVTKDWGFWEFAGVQACRGVGVMIAMIATQQ
ITMSTLPPHMVKNASGLVNLSRNTGGAIGLALLATSITQQTALYYDDLTSKVTQGSAAAGMIAGLTERMMQLGVADPAGA
ARKAVGGMMQQQATVMAFGDSFTLLAIGCFIAAAVSLLAAPVKNAPPPPSDAH

Sequences:

>Translated_533_residues
MADAALPADSPSAPATPGATAPVDWTKLFLGFGAMVIGQFMAILDIQIVAASLPQIQAGVGASTDQVSWIQTAYLIPEVV
MIPLSGYLSRLWGTQKLYLASCAGFIVMSILTGLSTSIDMMIITRALQGFIGGAMIPTVFAVAFTAFPPERRVTASVIMG
LIVTLAPTVGPTLGGHLTEWLSWRWLFFINVPTGLIVLFGVARWGDFDKGDPSLAKGFDWFGLAVMAVFLMSMQYVLEEG
AKEDWLSDSLILWLTVVAVFGGVVFVWRQLTYRNPIVELRAFANRNFTVGVLMTAVSGASLFGGTFLLPQFLGRVRHYSA
SEVGTTMVISGLSMFLTGPIAGRLVRKTDPRLPMCVGFLLAGFGMYMAHGVTKDWGFWEFAGVQACRGVGVMIAMIATQQ
ITMSTLPPHMVKNASGLVNLSRNTGGAIGLALLATSITQQTALYYDDLTSKVTQGSAAAGMIAGLTERMMQLGVADPAGA
ARKAVGGMMQQQATVMAFGDSFTLLAIGCFIAAAVSLLAAPVKNAPPPPSDAH
>Mature_532_residues
ADAALPADSPSAPATPGATAPVDWTKLFLGFGAMVIGQFMAILDIQIVAASLPQIQAGVGASTDQVSWIQTAYLIPEVVM
IPLSGYLSRLWGTQKLYLASCAGFIVMSILTGLSTSIDMMIITRALQGFIGGAMIPTVFAVAFTAFPPERRVTASVIMGL
IVTLAPTVGPTLGGHLTEWLSWRWLFFINVPTGLIVLFGVARWGDFDKGDPSLAKGFDWFGLAVMAVFLMSMQYVLEEGA
KEDWLSDSLILWLTVVAVFGGVVFVWRQLTYRNPIVELRAFANRNFTVGVLMTAVSGASLFGGTFLLPQFLGRVRHYSAS
EVGTTMVISGLSMFLTGPIAGRLVRKTDPRLPMCVGFLLAGFGMYMAHGVTKDWGFWEFAGVQACRGVGVMIAMIATQQI
TMSTLPPHMVKNASGLVNLSRNTGGAIGLALLATSITQQTALYYDDLTSKVTQGSAAAGMIAGLTERMMQLGVADPAGAA
RKAVGGMMQQQATVMAFGDSFTLLAIGCFIAAAVSLLAAPVKNAPPPPSDAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=474, Percent_Identity=30.5907172995781, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788710, Length=496, Percent_Identity=29.4354838709677, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081983, Length=406, Percent_Identity=25.615763546798, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790195, Length=335, Percent_Identity=24.4776119402985, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1788392, Length=254, Percent_Identity=28.740157480315, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=31.0077519379845, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322958, Length=167, Percent_Identity=26.3473053892216, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=151, Percent_Identity=27.8145695364238, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56506; Mature: 56375

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAALPADSPSAPATPGATAPVDWTKLFLGFGAMVIGQFMAILDIQIVAASLPQIQAGV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
GASTDQVSWIQTAYLIPEVVMIPLSGYLSRLWGTQKLYLASCAGFIVMSILTGLSTSIDM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
MIITRALQGFIGGAMIPTVFAVAFTAFPPERRVTASVIMGLIVTLAPTVGPTLGGHLTEW
HHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LSWRWLFFINVPTGLIVLFGVARWGDFDKGDPSLAKGFDWFGLAVMAVFLMSMQYVLEEG
HCEEEEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC
AKEDWLSDSLILWLTVVAVFGGVVFVWRQLTYRNPIVELRAFANRNFTVGVLMTAVSGAS
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCH
LFGGTFLLPQFLGRVRHYSASEVGTTMVISGLSMFLTGPIAGRLVRKTDPRLPMCVGFLL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHH
AGFGMYMAHGVTKDWGFWEFAGVQACRGVGVMIAMIATQQITMSTLPPHMVKNASGLVNL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEE
SRNTGGAIGLALLATSITQQTALYYDDLTSKVTQGSAAAGMIAGLTERMMQLGVADPAGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHH
ARKAVGGMMQQQATVMAFGDSFTLLAIGCFIAAAVSLLAAPVKNAPPPPSDAH
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ADAALPADSPSAPATPGATAPVDWTKLFLGFGAMVIGQFMAILDIQIVAASLPQIQAGV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
GASTDQVSWIQTAYLIPEVVMIPLSGYLSRLWGTQKLYLASCAGFIVMSILTGLSTSIDM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
MIITRALQGFIGGAMIPTVFAVAFTAFPPERRVTASVIMGLIVTLAPTVGPTLGGHLTEW
HHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LSWRWLFFINVPTGLIVLFGVARWGDFDKGDPSLAKGFDWFGLAVMAVFLMSMQYVLEEG
HCEEEEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCC
AKEDWLSDSLILWLTVVAVFGGVVFVWRQLTYRNPIVELRAFANRNFTVGVLMTAVSGAS
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCH
LFGGTFLLPQFLGRVRHYSASEVGTTMVISGLSMFLTGPIAGRLVRKTDPRLPMCVGFLL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHH
AGFGMYMAHGVTKDWGFWEFAGVQACRGVGVMIAMIATQQITMSTLPPHMVKNASGLVNL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEE
SRNTGGAIGLALLATSITQQTALYYDDLTSKVTQGSAAAGMIAGLTERMMQLGVADPAGA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHH
ARKAVGGMMQQQATVMAFGDSFTLLAIGCFIAAAVSLLAAPVKNAPPPPSDAH
HHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]