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The map label for this gene is leuB [H]

Identifier: 160903141

GI number: 160903141

Start: 1869592

End: 1870587

Strand: Direct

Name: leuB [H]

Synonym: Pmob_1706

Alternate gene names: 160903141

Gene position: 1869592-1870587 (Clockwise)

Preceding gene: 160903140

Following gene: 160903142

Centisome position: 86.17

GC content: 35.54

Gene sequence:

>996_bases
ATGTATACAGTTACTTTGATTCCGGGTGATGGAATAGGTCCAGAAATAACCTCTGTAGTGGTAGAGATTTTTGAACATTT
GAAAGCTCCTATTAGTTGGGATTTAGTGGAAGCTGGAGAGAAGGTAATTGAAAAATATGGAACTCCACTGCCTGATTATG
TGATCGATTCGATTAGGAAAAATAAAGTAGCCTTGAAAGGTCCTATAACTACGCCGATAGGCAAAGGATTTAGAAGTGTG
AACGTTACACTAAGAGAACGGTTCAATTTATATGCAAATTTAAGGCCTGTTAAAAGTTTAGAGGGTCTCAATACTAAGTA
CAATAATGTCGATTTGGTTGTAGTGAGAGAAAATACCGAATGTTTGTATAAAGGGATTGAGTATAAGATTGACGATACAG
CTTGTGCCGTCAGAGTTATAACAAAAAGCGCAAGTGAAAAGATCGCATATTTTGCATTTAATTATGCAAAGGAAAATAAA
AGAAAGAAAGTCACAGCTGTTCATAAGGCTAACATTCTAAAGATTACAGATGGCTTATTTTTGGAAGCTATTAGAAAAGT
AGCAAATGAATATCCTGAAATAGAGTACGAAGAAAAGATCATTGACAATATGTCAATGCAGTTGGTGTTGAATCCTGAAA
AATTTGATGTAGTTGTAGCCCCAAATTTGTACGGAGATATCCTTTCCGATTTAGCTGCAGGTTTAATAGGAGGATTGGGT
CTTGCTCCAGCTGCTAATATTGGTGAGGATACTGCTATCTTTGAGGCAGTCCATGGGAGTGCTCCGGATATAGCTAATAA
AGGTATAGCAAACCCGATCGCCTTATTAAGCTCATCCATTATGTTGTTAGATTATTTAAAGCTGAACGAGTTAGCAAAAA
AACTCGAAAACGCTATTTCCTCAACAGTTAAAGATATAGATAGTTTAACCCCGGATTTAGGTGGAAAAGGTAACATAGAG
TCTATAAAAAATAAAATTATTTCATTTTTGAATTGA

Upstream 100 bases:

>100_bases
CTCTCCCCAAAGTGATGCTAAAGATTTTACAAAGTGGGGGTTTGGTTAATTATTACAAAAAATATGGTACTTTAGAATTA
ATGGAATAGTAGGTGATTTT

Downstream 100 bases:

>100_bases
ATGGTTGGTGATTTTTTTGAAAGATAGAATTTATAAGATAGTTATAAAGGTTGGTAGTAGTTCAATAGCCGATGAGAATG
GAATAAATGTTGGTAAGATG

Product: isocitrate dehydrogenase (NAD(+))

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MYTVTLIPGDGIGPEITSVVVEIFEHLKAPISWDLVEAGEKVIEKYGTPLPDYVIDSIRKNKVALKGPITTPIGKGFRSV
NVTLRERFNLYANLRPVKSLEGLNTKYNNVDLVVVRENTECLYKGIEYKIDDTACAVRVITKSASEKIAYFAFNYAKENK
RKKVTAVHKANILKITDGLFLEAIRKVANEYPEIEYEEKIIDNMSMQLVLNPEKFDVVVAPNLYGDILSDLAAGLIGGLG
LAPAANIGEDTAIFEAVHGSAPDIANKGIANPIALLSSSIMLLDYLKLNELAKKLENAISSTVKDIDSLTPDLGGKGNIE
SIKNKIISFLN

Sequences:

>Translated_331_residues
MYTVTLIPGDGIGPEITSVVVEIFEHLKAPISWDLVEAGEKVIEKYGTPLPDYVIDSIRKNKVALKGPITTPIGKGFRSV
NVTLRERFNLYANLRPVKSLEGLNTKYNNVDLVVVRENTECLYKGIEYKIDDTACAVRVITKSASEKIAYFAFNYAKENK
RKKVTAVHKANILKITDGLFLEAIRKVANEYPEIEYEEKIIDNMSMQLVLNPEKFDVVVAPNLYGDILSDLAAGLIGGLG
LAPAANIGEDTAIFEAVHGSAPDIANKGIANPIALLSSSIMLLDYLKLNELAKKLENAISSTVKDIDSLTPDLGGKGNIE
SIKNKIISFLN
>Mature_331_residues
MYTVTLIPGDGIGPEITSVVVEIFEHLKAPISWDLVEAGEKVIEKYGTPLPDYVIDSIRKNKVALKGPITTPIGKGFRSV
NVTLRERFNLYANLRPVKSLEGLNTKYNNVDLVVVRENTECLYKGIEYKIDDTACAVRVITKSASEKIAYFAFNYAKENK
RKKVTAVHKANILKITDGLFLEAIRKVANEYPEIEYEEKIIDNMSMQLVLNPEKFDVVVAPNLYGDILSDLAAGLIGGLG
LAPAANIGEDTAIFEAVHGSAPDIANKGIANPIALLSSSIMLLDYLKLNELAKKLENAISSTVKDIDSLTPDLGGKGNIE
SIKNKIISFLN

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=329, Percent_Identity=50.4559270516717, Blast_Score=327, Evalue=7e-90,
Organism=Homo sapiens, GI4758582, Length=330, Percent_Identity=44.2424242424242, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI28178838, Length=310, Percent_Identity=44.5161290322581, Blast_Score=256, Evalue=1e-68,
Organism=Homo sapiens, GI28178816, Length=341, Percent_Identity=40.4692082111437, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI28178821, Length=342, Percent_Identity=40.3508771929825, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI28178819, Length=198, Percent_Identity=42.9292929292929, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1787381, Length=358, Percent_Identity=32.9608938547486, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788101, Length=353, Percent_Identity=32.0113314447592, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI87081683, Length=330, Percent_Identity=33.9393939393939, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71986051, Length=327, Percent_Identity=50.4587155963303, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI25144293, Length=337, Percent_Identity=39.4658753709199, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17505779, Length=330, Percent_Identity=38.1818181818182, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17550882, Length=320, Percent_Identity=40.625, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324709, Length=334, Percent_Identity=47.3053892215569, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6324291, Length=334, Percent_Identity=41.9161676646707, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=40.6876790830946, Blast_Score=246, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=29.9168975069252, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24643270, Length=330, Percent_Identity=52.1212121212121, Blast_Score=359, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24643268, Length=330, Percent_Identity=52.1212121212121, Blast_Score=358, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24661184, Length=334, Percent_Identity=47.0059880239521, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI161078637, Length=333, Percent_Identity=39.3393393393393, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI161078635, Length=333, Percent_Identity=39.3393393393393, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI161078633, Length=333, Percent_Identity=39.3393393393393, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24650122, Length=333, Percent_Identity=39.3393393393393, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI161078639, Length=328, Percent_Identity=39.3292682926829, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI281362242, Length=332, Percent_Identity=40.6626506024096, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24648872, Length=332, Percent_Identity=40.6626506024096, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI20130355, Length=329, Percent_Identity=33.7386018237082, Blast_Score=211, Evalue=7e-55,
Organism=Drosophila melanogaster, GI45550436, Length=332, Percent_Identity=22.5903614457831, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: 8180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36255; Mature: 36255

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTVTLIPGDGIGPEITSVVVEIFEHLKAPISWDLVEAGEKVIEKYGTPLPDYVIDSIRK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHC
NKVALKGPITTPIGKGFRSVNVTLRERFNLYANLRPVKSLEGLNTKYNNVDLVVVRENTE
CCEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEECCCH
CLYKGIEYKIDDTACAVRVITKSASEKIAYFAFNYAKENKRKKVTAVHKANILKITDGLF
HHHCCCCEEECCHHHHHHHHHHHHCHHEEHEEEHHHHHCCHHHHHHHHHCCEEEECHHHH
LEAIRKVANEYPEIEYEEKIIDNMSMQLVLNPEKFDVVVAPNLYGDILSDLAAGLIGGLG
HHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCC
LAPAANIGEDTAIFEAVHGSAPDIANKGIANPIALLSSSIMLLDYLKLNELAKKLENAIS
CCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STVKDIDSLTPDLGGKGNIESIKNKIISFLN
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MYTVTLIPGDGIGPEITSVVVEIFEHLKAPISWDLVEAGEKVIEKYGTPLPDYVIDSIRK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHC
NKVALKGPITTPIGKGFRSVNVTLRERFNLYANLRPVKSLEGLNTKYNNVDLVVVRENTE
CCEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEEECCCH
CLYKGIEYKIDDTACAVRVITKSASEKIAYFAFNYAKENKRKKVTAVHKANILKITDGLF
HHHCCCCEEECCHHHHHHHHHHHHCHHEEHEEEHHHHHCCHHHHHHHHHCCEEEECHHHH
LEAIRKVANEYPEIEYEEKIIDNMSMQLVLNPEKFDVVVAPNLYGDILSDLAAGLIGGLG
HHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCC
LAPAANIGEDTAIFEAVHGSAPDIANKGIANPIALLSSSIMLLDYLKLNELAKKLENAIS
CCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STVKDIDSLTPDLGGKGNIESIKNKIISFLN
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]