Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 160903142

GI number: 160903142

Start: 1870604

End: 1871770

Strand: Direct

Name: proB [H]

Synonym: Pmob_1707

Alternate gene names: 160903142

Gene position: 1870604-1871770 (Clockwise)

Preceding gene: 160903141

Following gene: 160903144

Centisome position: 86.22

GC content: 35.39

Gene sequence:

>1167_bases
TTGAAAGATAGAATTTATAAGATAGTTATAAAGGTTGGTAGTAGTTCAATAGCCGATGAGAATGGAATAAATGTTGGTAA
GATGAATAAAATCGTCGAGCAAGTGACTGAATTAAAGTTTAAAAGAAAAAAAGATATAATTATTGTTTCATCAGGAGCTA
TTGCCGCTGGCAAAAGTGAGTTAGGTTACCGTTATAAACCTTATTCTGTTTCTGAAAAGCAGGCTTGTGCAGCAGTTGGT
CAAGGTGTGTTGATCTCTAAATATCGTGATCTTTTTGCAGTTAAAAATATTAAAGTGGCTCAAATACTTTTAACGGCTGA
CGATTTTTCTAATCGTCAGCGTTATTTAAATGCATACAATACGATGAATGCACTTTTAGAAAATGATGTAGTACCTATTG
TAAATGAAAACGATACAGTAACGACAGATGAAATTAAATTAGGCGATAATGATGTTTTGTCTGCTCAAGTTGCAAGCTTG
TTGGAAGCAGACTTATTGATCATTCTCTCGGATATTCCAGGATTATTAAAAAATTTAGGGAACCCGAAATCGCTGATTAA
TATTGTTGAAGAAGTTACCCCCGAGCTTGAAAATCTTGCCAACGGAAAGAGTGGCAAACTTGGTACTGGAGGAATGAGTT
CAAAATTAAAAGCAGCAAAGATTTCCAATGCTTCAGGAATACCTTTAACAATTCTCCCATCTTACGAAGAGGGTATCATC
ACAAAAGCTGTTGATAATATTGAAAATACGAAGTTCAACATTGGTACAACCTTTTTACCTAAAGATAAAAAGATGAGCAA
AAGGAAAAGGTGGATTCAGTTCAGCTTAAAGCCAAAAGGAGAATTGATTGTGGATAATGGAGCTCGCGAGGCACTTTTAA
AAGGAAAAAGTTTGTTAGCGGTAGGAATTGTAAGTTTAACAGGAAATTTTTCCATCGGAGATCTGGTGATAGTCTCCAAT
AAAAAAGGAGAAAATATCGGAAGGGGTTTGGTAAACTATTCATCAAAGGAATTGAAAGAAATAATTGGTAAGAAAACCAA
TGAAATTCTTACGTTCAAAGACAACGTTGGTCCTGAAGAAGTAATACACAGGGATAATTTAGTTATAATGGAGGCAACGG
AGGGATTTGAACCCTCGAATAACGGTTTTGCAGACCGCCGCCTTTGA

Upstream 100 bases:

>100_bases
GATATAGATAGTTTAACCCCGGATTTAGGTGGAAAAGGTAACATAGAGTCTATAAAAAATAAAATTATTTCATTTTTGAA
TTGAATGGTTGGTGATTTTT

Downstream 100 bases:

>100_bases
CCCCTTGGCTACGTTGCCAAATTGGCGGGGACGACGGGACTCGAACCCGCGGCCACCGGTGTGACAGACCGGCATGATAA
CCAACTTCACCACGTCCCCT

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKDRIYKIVIKVGSSSIADENGINVGKMNKIVEQVTELKFKRKKDIIIVSSGAIAAGKSELGYRYKPYSVSEKQACAAVG
QGVLISKYRDLFAVKNIKVAQILLTADDFSNRQRYLNAYNTMNALLENDVVPIVNENDTVTTDEIKLGDNDVLSAQVASL
LEADLLIILSDIPGLLKNLGNPKSLINIVEEVTPELENLANGKSGKLGTGGMSSKLKAAKISNASGIPLTILPSYEEGII
TKAVDNIENTKFNIGTTFLPKDKKMSKRKRWIQFSLKPKGELIVDNGAREALLKGKSLLAVGIVSLTGNFSIGDLVIVSN
KKGENIGRGLVNYSSKELKEIIGKKTNEILTFKDNVGPEEVIHRDNLVIMEATEGFEPSNNGFADRRL

Sequences:

>Translated_388_residues
MKDRIYKIVIKVGSSSIADENGINVGKMNKIVEQVTELKFKRKKDIIIVSSGAIAAGKSELGYRYKPYSVSEKQACAAVG
QGVLISKYRDLFAVKNIKVAQILLTADDFSNRQRYLNAYNTMNALLENDVVPIVNENDTVTTDEIKLGDNDVLSAQVASL
LEADLLIILSDIPGLLKNLGNPKSLINIVEEVTPELENLANGKSGKLGTGGMSSKLKAAKISNASGIPLTILPSYEEGII
TKAVDNIENTKFNIGTTFLPKDKKMSKRKRWIQFSLKPKGELIVDNGAREALLKGKSLLAVGIVSLTGNFSIGDLVIVSN
KKGENIGRGLVNYSSKELKEIIGKKTNEILTFKDNVGPEEVIHRDNLVIMEATEGFEPSNNGFADRRL
>Mature_388_residues
MKDRIYKIVIKVGSSSIADENGINVGKMNKIVEQVTELKFKRKKDIIIVSSGAIAAGKSELGYRYKPYSVSEKQACAAVG
QGVLISKYRDLFAVKNIKVAQILLTADDFSNRQRYLNAYNTMNALLENDVVPIVNENDTVTTDEIKLGDNDVLSAQVASL
LEADLLIILSDIPGLLKNLGNPKSLINIVEEVTPELENLANGKSGKLGTGGMSSKLKAAKISNASGIPLTILPSYEEGII
TKAVDNIENTKFNIGTTFLPKDKKMSKRKRWIQFSLKPKGELIVDNGAREALLKGKSLLAVGIVSLTGNFSIGDLVIVSN
KKGENIGRGLVNYSSKELKEIIGKKTNEILTFKDNVGPEEVIHRDNLVIMEATEGFEPSNNGFADRRL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=33.1034482758621, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=40.5994550408719, Blast_Score=246, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17569967, Length=270, Percent_Identity=35.1851851851852, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17569969, Length=270, Percent_Identity=35.1851851851852, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320506, Length=431, Percent_Identity=34.1067285382831, Blast_Score=192, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6321822, Length=423, Percent_Identity=34.9881796690307, Blast_Score=186, Evalue=4e-48,
Organism=Drosophila melanogaster, GI21357643, Length=286, Percent_Identity=33.5664335664336, Blast_Score=127, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 42296; Mature: 42296

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDRIYKIVIKVGSSSIADENGINVGKMNKIVEQVTELKFKRKKDIIIVSSGAIAAGKSE
CCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHH
LGYRYKPYSVSEKQACAAVGQGVLISKYRDLFAVKNIKVAQILLTADDFSNRQRYLNAYN
CCCEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHH
TMNALLENDVVPIVNENDTVTTDEIKLGDNDVLSAQVASLLEADLLIILSDIPGLLKNLG
HHHHHHHCCCEEEECCCCEEEECEEECCCCHHHHHHHHHHHHHHHHEEEHHHHHHHHHCC
NPKSLINIVEEVTPELENLANGKSGKLGTGGMSSKLKAAKISNASGIPLTILPSYEEGII
CHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCHH
TKAVDNIENTKFNIGTTFLPKDKKMSKRKRWIQFSLKPKGELIVDNGAREALLKGKSLLA
HHHHHCCCCCEEECCEEECCCCHHHHHHHCEEEEEECCCCCEEEECCHHHHHHCCCCEEE
VGIVSLTGNFSIGDLVIVSNKKGENIGRGLVNYSSKELKEIIGKKTNEILTFKDNVGPEE
EEEEEEECCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCHHH
VIHRDNLVIMEATEGFEPSNNGFADRRL
HHCCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKDRIYKIVIKVGSSSIADENGINVGKMNKIVEQVTELKFKRKKDIIIVSSGAIAAGKSE
CCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHH
LGYRYKPYSVSEKQACAAVGQGVLISKYRDLFAVKNIKVAQILLTADDFSNRQRYLNAYN
CCCEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHH
TMNALLENDVVPIVNENDTVTTDEIKLGDNDVLSAQVASLLEADLLIILSDIPGLLKNLG
HHHHHHHCCCEEEECCCCEEEECEEECCCCHHHHHHHHHHHHHHHHEEEHHHHHHHHHCC
NPKSLINIVEEVTPELENLANGKSGKLGTGGMSSKLKAAKISNASGIPLTILPSYEEGII
CHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCHH
TKAVDNIENTKFNIGTTFLPKDKKMSKRKRWIQFSLKPKGELIVDNGAREALLKGKSLLA
HHHHHCCCCCEEECCEEECCCCHHHHHHHCEEEEEECCCCCEEEECCHHHHHHCCCCEEE
VGIVSLTGNFSIGDLVIVSNKKGENIGRGLVNYSSKELKEIIGKKTNEILTFKDNVGPEE
EEEEEEECCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCHHH
VIHRDNLVIMEATEGFEPSNNGFADRRL
HHCCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA