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The map label for this gene is appF [H]

Identifier: 160902772

GI number: 160902772

Start: 1417530

End: 1418738

Strand: Direct

Name: appF [H]

Synonym: Pmob_1325

Alternate gene names: 160902772

Gene position: 1417530-1418738 (Clockwise)

Preceding gene: 160902771

Following gene: 160902773

Centisome position: 65.34

GC content: 34.99

Gene sequence:

>1209_bases
ATGCTTTTAAAAATCCGTAATCTGAAAAAGTATTTTCCAATTAAACAGGGGTTTTTGATAGATAGAACGGTCGGGTACAT
TAAAGCAGTGGATGGGGTCAATTTGGACTTAGATATAAATGAGACTTATGGATTGGTTGGAGAGTCAGGTTGTGGAAAGA
CCACGATTGGTAAAACGATTTTGAGATTGGAAGATCCTACTTCGGGGAAGATTTTTTTAAACGGAGAAGAAACTTCTCAT
TATTTTATGAATAAAAGAGCTGGAGCAAAGTATTTGGAAAGGGAATACATCGAATACGCTCATGAACTAGAAAAAGAGTT
AGGGAGCAAAGAGAAGGTTCTTGAAAGTCTTGAAGAAGAAGAGAGAAAGTATATACAATATTACTATGATAATGGAAAAG
AAAAATTTTTGGATTATATGTTTAAAGATATAGATAGAAAAAGAGCAGATTTCAGAAGAAATGTTCAAATAGTGTTTCAG
GATCCTACATCTTCTTTGAATCCAAGAATGACTGTAGGACAGACGTTAATTGAACCACTGTTGTTTCACAAGATGGCAAA
AAATAAAATAGAGGCAAAAAATATCGCCCTTGATATGTTGAAAAAAGTTGGTTTGAAACCTTACCATGCTGACAGATATC
CTCATCAATTTTCTGGTGGGCAAAGACAGAGGATTGCCGTAGCACGTGCGATTATTTTAGACCCAAAGTTGATTATCTTG
GATGAACCTACTTCTGCGTTAGATGTTTCAGTACAGGCACAGATAATAAATCTTTTAAAGACTTTACAGAGTGAGTTCAA
CGTTGGATATCTCTTTATTTCTCATGATTTAGGGGTTGTTAGGTTCATTTCAAACCATGTTGGGGTAATGTACCTTGGAA
GATTAGTTGAGTTTGGAGACGGTTCAGAAATATTTGATAGAACCTTACATCCATATTCACAAGCTTTGCTAAATGCCGCT
CCTCTTCCAGATCCAAGAAAACGAAGAGATAGAAAAAAGTTTTTGGTTAAAGGTCAAGTACCAAGTCCAGTTAATAGGCC
TAAGGGATGCTTTTTCAACCCAAGATGTCCCTACGCTATGGATATATGTAAAAAAGATTACCCTGAATACTATAAAATAA
ACGAAAACCATTATGTTGCTTGTTTCTTATACAAAGAGGATAGAGATAGAGGTTTATCACAAGAAAAAGAGGGTAAGTTG
GTAAAATAA

Upstream 100 bases:

>100_bases
CAGAAGTTGAAGAAAATCATTACATAAGATGTTTCAATCCTATATCTGATACTACTCCTGTTCATTATGACGATGTGATA
GATAAGAAGGAGGCTCTTTG

Downstream 100 bases:

>100_bases
CTTAAAGGAGGGATTCACATGAGAAAGTTGTTGGTACTTTTAGTAGTAGCTTTAAGCGCTTTGACGATTTTTGCGCAAGA
AGCTTACAACCCCGAGTGGC

Product: oligopeptide/dipeptide ABC transporter ATPase

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLLKIRNLKKYFPIKQGFLIDRTVGYIKAVDGVNLDLDINETYGLVGESGCGKTTIGKTILRLEDPTSGKIFLNGEETSH
YFMNKRAGAKYLEREYIEYAHELEKELGSKEKVLESLEEEERKYIQYYYDNGKEKFLDYMFKDIDRKRADFRRNVQIVFQ
DPTSSLNPRMTVGQTLIEPLLFHKMAKNKIEAKNIALDMLKKVGLKPYHADRYPHQFSGGQRQRIAVARAIILDPKLIIL
DEPTSALDVSVQAQIINLLKTLQSEFNVGYLFISHDLGVVRFISNHVGVMYLGRLVEFGDGSEIFDRTLHPYSQALLNAA
PLPDPRKRRDRKKFLVKGQVPSPVNRPKGCFFNPRCPYAMDICKKDYPEYYKINENHYVACFLYKEDRDRGLSQEKEGKL
VK

Sequences:

>Translated_402_residues
MLLKIRNLKKYFPIKQGFLIDRTVGYIKAVDGVNLDLDINETYGLVGESGCGKTTIGKTILRLEDPTSGKIFLNGEETSH
YFMNKRAGAKYLEREYIEYAHELEKELGSKEKVLESLEEEERKYIQYYYDNGKEKFLDYMFKDIDRKRADFRRNVQIVFQ
DPTSSLNPRMTVGQTLIEPLLFHKMAKNKIEAKNIALDMLKKVGLKPYHADRYPHQFSGGQRQRIAVARAIILDPKLIIL
DEPTSALDVSVQAQIINLLKTLQSEFNVGYLFISHDLGVVRFISNHVGVMYLGRLVEFGDGSEIFDRTLHPYSQALLNAA
PLPDPRKRRDRKKFLVKGQVPSPVNRPKGCFFNPRCPYAMDICKKDYPEYYKINENHYVACFLYKEDRDRGLSQEKEGKL
VK
>Mature_402_residues
MLLKIRNLKKYFPIKQGFLIDRTVGYIKAVDGVNLDLDINETYGLVGESGCGKTTIGKTILRLEDPTSGKIFLNGEETSH
YFMNKRAGAKYLEREYIEYAHELEKELGSKEKVLESLEEEERKYIQYYYDNGKEKFLDYMFKDIDRKRADFRRNVQIVFQ
DPTSSLNPRMTVGQTLIEPLLFHKMAKNKIEAKNIALDMLKKVGLKPYHADRYPHQFSGGQRQRIAVARAIILDPKLIIL
DEPTSALDVSVQAQIINLLKTLQSEFNVGYLFISHDLGVVRFISNHVGVMYLGRLVEFGDGSEIFDRTLHPYSQALLNAA
PLPDPRKRRDRKKFLVKGQVPSPVNRPKGCFFNPRCPYAMDICKKDYPEYYKINENHYVACFLYKEDRDRGLSQEKEGKL
VK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG4608

COG function: function code E; ABC-type oligopeptide transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI1787500, Length=238, Percent_Identity=49.1596638655462, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1789962, Length=238, Percent_Identity=52.1008403361345, Blast_Score=241, Evalue=8e-65,
Organism=Escherichia coli, GI145693107, Length=344, Percent_Identity=38.953488372093, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1787758, Length=217, Percent_Identity=46.5437788018433, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI48994883, Length=255, Percent_Identity=43.1372549019608, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1789963, Length=262, Percent_Identity=42.3664122137405, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788506, Length=178, Percent_Identity=46.6292134831461, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1787759, Length=391, Percent_Identity=30.1790281329923, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787547, Length=316, Percent_Identity=29.1139240506329, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1789891, Length=191, Percent_Identity=42.9319371727749, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787548, Length=271, Percent_Identity=32.8413284132841, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1787089, Length=166, Percent_Identity=39.7590361445783, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994997, Length=182, Percent_Identity=34.6153846153846, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787370, Length=154, Percent_Identity=37.012987012987, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787712, Length=176, Percent_Identity=32.9545454545455, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786872, Length=162, Percent_Identity=33.9506172839506, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786398, Length=169, Percent_Identity=34.9112426035503, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788225, Length=286, Percent_Identity=26.2237762237762, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788644, Length=185, Percent_Identity=30.8108108108108, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789890, Length=169, Percent_Identity=34.3195266272189, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789672, Length=175, Percent_Identity=33.7142857142857, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789032, Length=233, Percent_Identity=30.0429184549356, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081709, Length=176, Percent_Identity=29.5454545454545, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081791, Length=173, Percent_Identity=31.2138728323699, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787029, Length=129, Percent_Identity=34.1085271317829, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081834, Length=144, Percent_Identity=36.8055555555556, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790162, Length=168, Percent_Identity=31.547619047619, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788761, Length=86, Percent_Identity=40.6976744186046, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082267, Length=201, Percent_Identity=28.3582089552239, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786563, Length=105, Percent_Identity=40.9523809523809, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788450, Length=179, Percent_Identity=25.1396648044693, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790467, Length=153, Percent_Identity=28.7581699346405, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787112, Length=110, Percent_Identity=36.3636363636364, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788165, Length=85, Percent_Identity=41.1764705882353, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1786253, Length=159, Percent_Identity=28.9308176100629, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787164, Length=93, Percent_Identity=35.4838709677419, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786345, Length=108, Percent_Identity=31.4814814814815, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17565938, Length=93, Percent_Identity=43.010752688172, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193211015, Length=182, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193211017, Length=182, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71996809, Length=182, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324985, Length=118, Percent_Identity=37.2881355932203, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24641565, Length=99, Percent_Identity=40.4040404040404, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR010066
- InterPro:   IPR013563 [H]

Pfam domain/function: PF00005 ABC_tran; PF08352 oligo_HPY [H]

EC number: NA

Molecular weight: Translated: 46515; Mature: 46515

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKIRNLKKYFPIKQGFLIDRTVGYIKAVDGVNLDLDINETYGLVGESGCGKTTIGKTI
CEEEECCHHHHCCCCCCEEEEHHHHHHEEECCCEEEEECCCCCCCCCCCCCCCHHHCCEE
LRLEDPTSGKIFLNGEETSHYFMNKRAGAKYLEREYIEYAHELEKELGSKEKVLESLEEE
EEECCCCCCEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ERKYIQYYYDNGKEKFLDYMFKDIDRKRADFRRNVQIVFQDPTSSLNPRMTVGQTLIEPL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHH
LFHKMAKNKIEAKNIALDMLKKVGLKPYHADRYPHQFSGGQRQRIAVARAIILDPKLIIL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCEEEEE
DEPTSALDVSVQAQIINLLKTLQSEFNVGYLFISHDLGVVRFISNHVGVMYLGRLVEFGD
ECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHCHHHHHHHHHHHCCC
GSEIFDRTLHPYSQALLNAAPLPDPRKRRDRKKFLVKGQVPSPVNRPKGCFFNPRCPYAM
CHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCHHH
DICKKDYPEYYKINENHYVACFLYKEDRDRGLSQEKEGKLVK
HHHHCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLLKIRNLKKYFPIKQGFLIDRTVGYIKAVDGVNLDLDINETYGLVGESGCGKTTIGKTI
CEEEECCHHHHCCCCCCEEEEHHHHHHEEECCCEEEEECCCCCCCCCCCCCCCHHHCCEE
LRLEDPTSGKIFLNGEETSHYFMNKRAGAKYLEREYIEYAHELEKELGSKEKVLESLEEE
EEECCCCCCEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ERKYIQYYYDNGKEKFLDYMFKDIDRKRADFRRNVQIVFQDPTSSLNPRMTVGQTLIEPL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHH
LFHKMAKNKIEAKNIALDMLKKVGLKPYHADRYPHQFSGGQRQRIAVARAIILDPKLIIL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCEEEEE
DEPTSALDVSVQAQIINLLKTLQSEFNVGYLFISHDLGVVRFISNHVGVMYLGRLVEFGD
ECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHCHHHHHHHHHHHCCC
GSEIFDRTLHPYSQALLNAAPLPDPRKRRDRKKFLVKGQVPSPVNRPKGCFFNPRCPYAM
CHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCHHH
DICKKDYPEYYKINENHYVACFLYKEDRDRGLSQEKEGKLVK
HHHHCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]