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The map label for this gene is nanE [H]

Identifier: 160901996

GI number: 160901996

Start: 565612

End: 567120

Strand: Direct

Name: nanE [H]

Synonym: Pmob_0520

Alternate gene names: 160901996

Gene position: 565612-567120 (Clockwise)

Preceding gene: 160901977

Following gene: 160901997

Centisome position: 26.07

GC content: 34.86

Gene sequence:

>1509_bases
ATGAAAATGTTGAAGAAGAATGCATTCATTGTATCCTGTCAAGCAGAAGAAGGGGAACCCCTATATGGGAAAGAAACTAT
TGTAAAGATGGCTATGGCGGCTCAGGCTGGAGGAGCAGACGCTATCAGGGCTCTTTCTCCTGAAAATATTCGAGAGATAA
AAAAAATTGTTAACCTTCCAGTAATAGGAATAACTAAAAACAGGAGTTTAAAAGGGGCATTTATCACAACCACTAAAAAA
GATATAGATAATTTAGTTGATGCGGACTCAGATTTTATTGCTATTGATTGTACAAGAAGAGAAAGACCTGAACCTTTACC
AGAACTTTTTGACTATTTAAGAAAATATTACCCCCACATTGGAATAATAGCGGATATAGCTGACATAGAAGATGTGAAAT
ATGTTTTACCTTTAAAGCCCGACTATATAGCAACTACACTCGCTGGTTATACTGATTATACCTCCGGAAGAGATTTACCA
GATTTAGATTTAATAGAAAAAACTGTAAAAATAACGAATATACCTGTTATCGCTGAAGGGAACATCTCTACTCCTCAATA
TGCAAGGCAAGCAATCTTATACGGAGCATATGCTGTTACTGTTGGATCGGCCATTACTAGGCCTCATGTAATTGCTAAAA
ATTTTAAAGATCACCTTAAGGACTTTAGAAATGATGAAATTTCTGCTGTAGGCATAGATATAGGAGGAACTTGGACTCGT
GGAGTTTTAACTGATAGATTTGGAGAAATTATAAAAAGTAAAAAGATACCTACAGCAGCAACAGGCAAAGAAGTTATTTC
AAATATGCTTTCTCTAATTAAAGGTTTAATAACGAATGAGACCCACTTTATGGGAATTGCTACCGGTGGAAAAATTAATT
TTAAGTCAGGAATTGTCAATTTTTCAACTGGTTTGATTCCTGATTGGGAAGGAGTACAAATTGCAGATATTGTAGAACAA
GAATTTCATATACGGCCAAAGGTTGATAATGATGCTAATTGTGCCGCATATTTCCAGCATTACATCACAAAAGTCAACAA
TTTATTGATGATAACCGTAGGGACAGGTCTTGGTGGAGGAATAATTATTAACGGGAAGATAATAAGAGGAATTATGGGAG
GTGGTGGAGAAATAGGTCACATAGTATATCCAGGAAATAACAAACGATGTACTTGTGGAAAAGTTGGTTGCGTTGAAACT
TTGCTTTCTGGAAGATACTTAAGAGAAAAAGTATATGAAAAGAATAATGAAATATCCCTAAATAATATAAAAGATTATGC
AAAAGTTATGGCTTGGTTAATTGATACAGTTAAAACTACCGTGGACTTTGAAAAATGTTACCTTGGTGGAGTCATTCCAA
AGTACGGTGAGAAAGTATTAAAGGAAATTCGGTCCTCTTATGAAAAAATAAGAAATGAAAGTGGCGAATTTATTGAATTT
AGTCAATTAGGTGAATTTGCAGGCGCAAGGGGATCGGCAATACTTTCATTTCAAAAAGGAGAAATCTAA

Upstream 100 bases:

>100_bases
TTTGACTTTTCAGAAAATAAGAATTATAATGAAAATGTAGATAAATTTCGTTATTTAGTTTTTTATATGAAAATTATTTT
CAATATTTGGAGGAAGATCT

Downstream 100 bases:

>100_bases
AAATGAGTAAAATTCAAGCTTTTCTTAATTCTAGGATGCCTACTTTCACTAAATCTGAAAAGAAAGTAGCGGAGTATATA
TTAAATAGTAAAAAAGAGGA

Product: putative N-acetylmannosamine-6-phosphate epimerase

Products: NA

Alternate protein names: ManNAc-6-P epimerase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKMLKKNAFIVSCQAEEGEPLYGKETIVKMAMAAQAGGADAIRALSPENIREIKKIVNLPVIGITKNRSLKGAFITTTKK
DIDNLVDADSDFIAIDCTRRERPEPLPELFDYLRKYYPHIGIIADIADIEDVKYVLPLKPDYIATTLAGYTDYTSGRDLP
DLDLIEKTVKITNIPVIAEGNISTPQYARQAILYGAYAVTVGSAITRPHVIAKNFKDHLKDFRNDEISAVGIDIGGTWTR
GVLTDRFGEIIKSKKIPTAATGKEVISNMLSLIKGLITNETHFMGIATGGKINFKSGIVNFSTGLIPDWEGVQIADIVEQ
EFHIRPKVDNDANCAAYFQHYITKVNNLLMITVGTGLGGGIIINGKIIRGIMGGGGEIGHIVYPGNNKRCTCGKVGCVET
LLSGRYLREKVYEKNNEISLNNIKDYAKVMAWLIDTVKTTVDFEKCYLGGVIPKYGEKVLKEIRSSYEKIRNESGEFIEF
SQLGEFAGARGSAILSFQKGEI

Sequences:

>Translated_502_residues
MKMLKKNAFIVSCQAEEGEPLYGKETIVKMAMAAQAGGADAIRALSPENIREIKKIVNLPVIGITKNRSLKGAFITTTKK
DIDNLVDADSDFIAIDCTRRERPEPLPELFDYLRKYYPHIGIIADIADIEDVKYVLPLKPDYIATTLAGYTDYTSGRDLP
DLDLIEKTVKITNIPVIAEGNISTPQYARQAILYGAYAVTVGSAITRPHVIAKNFKDHLKDFRNDEISAVGIDIGGTWTR
GVLTDRFGEIIKSKKIPTAATGKEVISNMLSLIKGLITNETHFMGIATGGKINFKSGIVNFSTGLIPDWEGVQIADIVEQ
EFHIRPKVDNDANCAAYFQHYITKVNNLLMITVGTGLGGGIIINGKIIRGIMGGGGEIGHIVYPGNNKRCTCGKVGCVET
LLSGRYLREKVYEKNNEISLNNIKDYAKVMAWLIDTVKTTVDFEKCYLGGVIPKYGEKVLKEIRSSYEKIRNESGEFIEF
SQLGEFAGARGSAILSFQKGEI
>Mature_502_residues
MKMLKKNAFIVSCQAEEGEPLYGKETIVKMAMAAQAGGADAIRALSPENIREIKKIVNLPVIGITKNRSLKGAFITTTKK
DIDNLVDADSDFIAIDCTRRERPEPLPELFDYLRKYYPHIGIIADIADIEDVKYVLPLKPDYIATTLAGYTDYTSGRDLP
DLDLIEKTVKITNIPVIAEGNISTPQYARQAILYGAYAVTVGSAITRPHVIAKNFKDHLKDFRNDEISAVGIDIGGTWTR
GVLTDRFGEIIKSKKIPTAATGKEVISNMLSLIKGLITNETHFMGIATGGKINFKSGIVNFSTGLIPDWEGVQIADIVEQ
EFHIRPKVDNDANCAAYFQHYITKVNNLLMITVGTGLGGGIIINGKIIRGIMGGGGEIGHIVYPGNNKRCTCGKVGCVET
LLSGRYLREKVYEKNNEISLNNIKDYAKVMAWLIDTVKTTVDFEKCYLGGVIPKYGEKVLKEIRSSYEKIRNESGEFIEF
SQLGEFAGARGSAILSFQKGEI

Specific function: Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) (Potential) [H]

COG id: COG3010

COG function: function code G; Putative N-acetylmannosamine-6-phosphate epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nanE family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=202, Percent_Identity=31.6831683168317, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI190014632, Length=202, Percent_Identity=31.6831683168317, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI4885285, Length=202, Percent_Identity=31.6831683168317, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI298566315, Length=202, Percent_Identity=31.6831683168317, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1789617, Length=223, Percent_Identity=38.5650224215247, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787363, Length=309, Percent_Identity=23.9482200647249, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790522, Length=304, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87082127, Length=150, Percent_Identity=28, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082230, Length=244, Percent_Identity=26.6393442622951, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786891, Length=194, Percent_Identity=24.2268041237113, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI87081733, Length=191, Percent_Identity=27.2251308900524, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR007260
- InterPro:   IPR011060 [H]

Pfam domain/function: PF04131 NanE [H]

EC number: =5.1.3.9 [H]

Molecular weight: Translated: 55248; Mature: 55248

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMLKKNAFIVSCQAEEGEPLYGKETIVKMAMAAQAGGADAIRALSPENIREIKKIVNLP
CCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHCCC
VIGITKNRSLKGAFITTTKKDIDNLVDADSDFIAIDCTRRERPEPLPELFDYLRKYYPHI
EEEEECCCCCCCEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCC
GIIADIADIEDVKYVLPLKPDYIATTLAGYTDYTSGRDLPDLDLIEKTVKITNIPVIAEG
EEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEECCEEEECC
NISTPQYARQAILYGAYAVTVGSAITRPHVIAKNFKDHLKDFRNDEISAVGIDIGGTWTR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHH
GVLTDRFGEIIKSKKIPTAATGKEVISNMLSLIKGLITNETHFMGIATGGKINFKSGIVN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCEE
FSTGLIPDWEGVQIADIVEQEFHIRPKVDNDANCAAYFQHYITKVNNLLMITVGTGLGGG
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCC
IIINGKIIRGIMGGGGEIGHIVYPGNNKRCTCGKVGCVETLLSGRYLREKVYEKNNEISL
EEECCEEEEEEECCCCCEEEEEECCCCCEEECCCHHHHHHHHCCHHHHHHHHCCCCCEEH
NNIKDYAKVMAWLIDTVKTTVDFEKCYLGGVIPKYGEKVLKEIRSSYEKIRNESGEFIEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEH
SQLGEFAGARGSAILSFQKGEI
HHHHHHCCCCCCEEEEECCCCC
>Mature Secondary Structure
MKMLKKNAFIVSCQAEEGEPLYGKETIVKMAMAAQAGGADAIRALSPENIREIKKIVNLP
CCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHCCC
VIGITKNRSLKGAFITTTKKDIDNLVDADSDFIAIDCTRRERPEPLPELFDYLRKYYPHI
EEEEECCCCCCCEEEECCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCC
GIIADIADIEDVKYVLPLKPDYIATTLAGYTDYTSGRDLPDLDLIEKTVKITNIPVIAEG
EEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEECCEEEECC
NISTPQYARQAILYGAYAVTVGSAITRPHVIAKNFKDHLKDFRNDEISAVGIDIGGTWTR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHH
GVLTDRFGEIIKSKKIPTAATGKEVISNMLSLIKGLITNETHFMGIATGGKINFKSGIVN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCEE
FSTGLIPDWEGVQIADIVEQEFHIRPKVDNDANCAAYFQHYITKVNNLLMITVGTGLGGG
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCC
IIINGKIIRGIMGGGGEIGHIVYPGNNKRCTCGKVGCVETLLSGRYLREKVYEKNNEISL
EEECCEEEEEEECCCCCEEEEEECCCCCEEECCCHHHHHHHHCCHHHHHHHHCCCCCEEH
NNIKDYAKVMAWLIDTVKTTVDFEKCYLGGVIPKYGEKVLKEIRSSYEKIRNESGEFIEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEH
SQLGEFAGARGSAILSFQKGEI
HHHHHHCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]