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The map label for this gene is ebgA [C]

Identifier: 160901973

GI number: 160901973

Start: 540297

End: 542678

Strand: Direct

Name: ebgA [C]

Synonym: Pmob_0497

Alternate gene names: 160901973

Gene position: 540297-542678 (Clockwise)

Preceding gene: 160901972

Following gene: 160901974

Centisome position: 24.9

GC content: 34.13

Gene sequence:

>2382_bases
TTGAAAATATCTTTAAATGGAAAATGGAAGGTTTACGATAACGAAAAAGAGTTTGAGTTTGATGGAAACGTTCCTGGAAC
CGTACAAGGAGACCTTGTGGATTTGAATCTCATGCCTCACCCTTATGTAGGAGAAAATGAAAAACTTTTCAAAAGGTTAG
AATGGAAAAATTGGATATACGAGAAGAAATTTCACATAGAAGAGATAAACGATGAGCTACGTTACGATCTTGTATTAGAG
GGTGTGGATACCCTTTCAAATATTTACCTTAACGATAATTTTGTTGGAGAAACCGAGGATATGTTCATAGAATACCGGTT
TGATATCAAAAAGTATTTGAAAATTGGAGAAAATTCTTTAAAAATAGAGATTAAATCTCCCATAGATCTCCCTAAAAGAT
TTGAAAAAAATTATGGGAAATTACACTCTGGCGAAGAAACCGCTAGAGTTTACATAAGAAAAGCTCAGTATTCCTATGGC
TGGGACTGGGGAGCAAGAATAGCTACAAGCGGGATATACAGAGATATATACATAGAAAGTTACAAAGATGCTAGACTTTT
TGGTTCCACCGCTTTTTTGGAGACTTTAGATGGAAAAGTTAACTTTTCCGGTTACATTGATTCGTGTATCAACTATACTA
AAAACTACGAGGTAGAAATCCTTTTCAATGGAAATCCAGTAATTACATTACCAGTAACCACTAGTGTTGAAAATTACAAG
TTTGAAGGTTCTAAAAGAATAGAGAATTTAAAGCTTTGGTATCCACACGATTTGGGTGAAAGTTATTTATACGAAGTTGA
GTTCAGACTTAAGGAAAATAAAGAAGTAATCTATGGTGAAAAAAAGAAAATAGGTTTTAGAATCGTCAAAGTAGTGAGAG
AAAATGATTCAGAAGGAGAAAGCTTTATCTTCGAGATAAACGGCAGAAAAATCTTCGCCAAAGGCGCAAATTGGATACCT
GCTGAAAATATCTTAAGTTGGTTAAAAGGGAGTGATTACGATAAACTTCTCAAAATGGCGAAGGAATCCAATATGAACAT
GTTGAGAGTATGGGGAGGTGGCCTTTACGAAGATCCTGCATTTTACAACAGATGTGATGAGTTAGGAATATTAGTTTGGC
AAGACTTCATGTTTGCATGTGCGGAATATCCTGACCATATAGATTGGTTTAGAAAGCTTGCAAATGAAGAAGTAAAACAT
CAAGTATTGAAACTAAGACACCATCCAAGTATAGTCCTTTGGTGCGGAAATAATGAGAATAATTGGGGCTTTGAAGAATG
GGACTACAAATTGAAAGTGGATGGGAAAAATCTTGGAAATAGACTTTATTTGGAAGATTTTCCTAAAATATGTGCTCAAG
AAGATCCATCGAGACTGTACTGGCCATCAAGCCCATACGGTGGCAGTAGAGCTAACTCTTCTGAAGCAGGAGACAGACAT
GTTTGGGAAATCTGGTCCGGATGGCAAGATTATAAATACTATACATGGGACACTTCAAAGTTCGTAAGTGAATTTGGATT
TCAAGCGGCTCCAGATCCGAAAACTATTGATTTCTTTGCAGAAGACAAAGAAAAAGATATATTTTCACCAACGATGTTAA
ATCATAATAAACAAGTTGAAGGTCCCGAAAGACTCTTAAGGTTCATCAATGGGCATTATGGATTGATTGACAACTTTGAT
TCAATTGTTTATTTAACTCAACTCAACCAAGCAGAGGCTATTAAAACAGGAGTAGAACACTGGAGAAACAGAAAATACAA
AACAGCCGGAACTCTTTATTGGCAAATAAATGATTCATGGCCTGTTTTCAGTTGGGCTTCAATAGATTATTTTAAAAGGC
CCAAGCCTTTATACTTTTACACTAAAAGATTTTATAATCAATTGTTAGCGATTGCCAAAAATAAAGATGAAAAGGTCATA
ATATCACTGATAAACGATGGAATCAAAATCAAACTTGATTTGGAATTTCAACTTTGGTCTCTAAATGGGAAAAAACTTAT
GCAAAAAGAATATTCAAACATAAAAACGTCCGAAGATTCTGTAATAACTGTTGATCAACTCAATATTTCCAACATTGACT
TAGATAACTCCATAGCTTACTTAATTCTAAAACAAAATGGAAAAACCATAATAGAAAACCACGAATTATTTGCAGATTTA
AGAAAAAATAAATTAAACGATCCAAAGATTACATACGAAAAAGAAGGAAACAACTTGATATTGAGTTGTGAAAAACCGGC
GTGGGGAGTAAACGTTAGGGTAAATGGAGAAAATTATTCTCAAGATAACTTCTTTACTCTTTTTCCATCCTATCAAAAAG
TTTTAAATAATATTGAAGGGGAAATTAGCATAAAAAGCGCCTTTGATTATTTGAGGAGATGA

Upstream 100 bases:

>100_bases
AAAATATAAAATCATCAAAGAGACGCTATATATAAGGTTTTAGAGTTTTTAAGGTTTTAAAGACAGTACCAAAAAATATT
AATAGGAGGAGAAATTTCAA

Downstream 100 bases:

>100_bases
GTATTATGGATTTTAATTTTGATCATTTAGCAATCACAGTTTCCGATTTGGAAAAATCTGTTGGATTTTACAGAGATATT
ATGGGTTTTAGAGTTTTAGG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Glycoside Hydrolase Family Protein; Beta-Mannosidase Protein; Glycoside Hydrolase; Mannosidase; Coagulation Factor 5/8 Type Domain Protein; Glycoside Hydrolase Family 2 TIM Barrel; Glycoside Hydrolase Family 2 Protein; Glycosidase; Beta-Galactosidase; Glycosyl Hydrolase; Beta-Galactosidase/Beta-Glucuronidase; Beta-Glucuronidase; Exported Glycosyl Hydrolase; Beta-Galactosidase/Beta- Glucuronidase Family Protein; O-Glycosyl Hydrolase; Beta-D-Mannosidase; Beta-Mannosidase Man2A; DS Domain/Family 2 Glycosyl Hydrolase; Beta-Galactosidase/Beta; Mannosylglycoprotein Endo-Beta-Mannosidase; Beta-Mannosidase-Related Protein; BETA-Mannosidase Protein

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MKISLNGKWKVYDNEKEFEFDGNVPGTVQGDLVDLNLMPHPYVGENEKLFKRLEWKNWIYEKKFHIEEINDELRYDLVLE
GVDTLSNIYLNDNFVGETEDMFIEYRFDIKKYLKIGENSLKIEIKSPIDLPKRFEKNYGKLHSGEETARVYIRKAQYSYG
WDWGARIATSGIYRDIYIESYKDARLFGSTAFLETLDGKVNFSGYIDSCINYTKNYEVEILFNGNPVITLPVTTSVENYK
FEGSKRIENLKLWYPHDLGESYLYEVEFRLKENKEVIYGEKKKIGFRIVKVVRENDSEGESFIFEINGRKIFAKGANWIP
AENILSWLKGSDYDKLLKMAKESNMNMLRVWGGGLYEDPAFYNRCDELGILVWQDFMFACAEYPDHIDWFRKLANEEVKH
QVLKLRHHPSIVLWCGNNENNWGFEEWDYKLKVDGKNLGNRLYLEDFPKICAQEDPSRLYWPSSPYGGSRANSSEAGDRH
VWEIWSGWQDYKYYTWDTSKFVSEFGFQAAPDPKTIDFFAEDKEKDIFSPTMLNHNKQVEGPERLLRFINGHYGLIDNFD
SIVYLTQLNQAEAIKTGVEHWRNRKYKTAGTLYWQINDSWPVFSWASIDYFKRPKPLYFYTKRFYNQLLAIAKNKDEKVI
ISLINDGIKIKLDLEFQLWSLNGKKLMQKEYSNIKTSEDSVITVDQLNISNIDLDNSIAYLILKQNGKTIIENHELFADL
RKNKLNDPKITYEKEGNNLILSCEKPAWGVNVRVNGENYSQDNFFTLFPSYQKVLNNIEGEISIKSAFDYLRR

Sequences:

>Translated_793_residues
MKISLNGKWKVYDNEKEFEFDGNVPGTVQGDLVDLNLMPHPYVGENEKLFKRLEWKNWIYEKKFHIEEINDELRYDLVLE
GVDTLSNIYLNDNFVGETEDMFIEYRFDIKKYLKIGENSLKIEIKSPIDLPKRFEKNYGKLHSGEETARVYIRKAQYSYG
WDWGARIATSGIYRDIYIESYKDARLFGSTAFLETLDGKVNFSGYIDSCINYTKNYEVEILFNGNPVITLPVTTSVENYK
FEGSKRIENLKLWYPHDLGESYLYEVEFRLKENKEVIYGEKKKIGFRIVKVVRENDSEGESFIFEINGRKIFAKGANWIP
AENILSWLKGSDYDKLLKMAKESNMNMLRVWGGGLYEDPAFYNRCDELGILVWQDFMFACAEYPDHIDWFRKLANEEVKH
QVLKLRHHPSIVLWCGNNENNWGFEEWDYKLKVDGKNLGNRLYLEDFPKICAQEDPSRLYWPSSPYGGSRANSSEAGDRH
VWEIWSGWQDYKYYTWDTSKFVSEFGFQAAPDPKTIDFFAEDKEKDIFSPTMLNHNKQVEGPERLLRFINGHYGLIDNFD
SIVYLTQLNQAEAIKTGVEHWRNRKYKTAGTLYWQINDSWPVFSWASIDYFKRPKPLYFYTKRFYNQLLAIAKNKDEKVI
ISLINDGIKIKLDLEFQLWSLNGKKLMQKEYSNIKTSEDSVITVDQLNISNIDLDNSIAYLILKQNGKTIIENHELFADL
RKNKLNDPKITYEKEGNNLILSCEKPAWGVNVRVNGENYSQDNFFTLFPSYQKVLNNIEGEISIKSAFDYLRR
>Mature_793_residues
MKISLNGKWKVYDNEKEFEFDGNVPGTVQGDLVDLNLMPHPYVGENEKLFKRLEWKNWIYEKKFHIEEINDELRYDLVLE
GVDTLSNIYLNDNFVGETEDMFIEYRFDIKKYLKIGENSLKIEIKSPIDLPKRFEKNYGKLHSGEETARVYIRKAQYSYG
WDWGARIATSGIYRDIYIESYKDARLFGSTAFLETLDGKVNFSGYIDSCINYTKNYEVEILFNGNPVITLPVTTSVENYK
FEGSKRIENLKLWYPHDLGESYLYEVEFRLKENKEVIYGEKKKIGFRIVKVVRENDSEGESFIFEINGRKIFAKGANWIP
AENILSWLKGSDYDKLLKMAKESNMNMLRVWGGGLYEDPAFYNRCDELGILVWQDFMFACAEYPDHIDWFRKLANEEVKH
QVLKLRHHPSIVLWCGNNENNWGFEEWDYKLKVDGKNLGNRLYLEDFPKICAQEDPSRLYWPSSPYGGSRANSSEAGDRH
VWEIWSGWQDYKYYTWDTSKFVSEFGFQAAPDPKTIDFFAEDKEKDIFSPTMLNHNKQVEGPERLLRFINGHYGLIDNFD
SIVYLTQLNQAEAIKTGVEHWRNRKYKTAGTLYWQINDSWPVFSWASIDYFKRPKPLYFYTKRFYNQLLAIAKNKDEKVI
ISLINDGIKIKLDLEFQLWSLNGKKLMQKEYSNIKTSEDSVITVDQLNISNIDLDNSIAYLILKQNGKTIIENHELFADL
RKNKLNDPKITYEKEGNNLILSCEKPAWGVNVRVNGENYSQDNFFTLFPSYQKVLNNIEGEISIKSAFDYLRR

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI84798622, Length=724, Percent_Identity=30.3867403314917, Blast_Score=315, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17550784, Length=719, Percent_Identity=27.3991655076495, Blast_Score=247, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24643838, Length=779, Percent_Identity=26.9576379974326, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24643840, Length=721, Percent_Identity=26.9070735090153, Blast_Score=230, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.1.25

Molecular weight: Translated: 93255; Mature: 93255

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLNGKWKVYDNEKEFEFDGNVPGTVQGDLVDLNLMPHPYVGENEKLFKRLEWKNWIY
CEEEECCEEEEECCCCCEEECCCCCCCEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
EKKFHIEEINDELRYDLVLEGVDTLSNIYLNDNFVGETEDMFIEYRFDIKKYLKIGENSL
HCEEEHHHHCCHHEEEEEEECHHHHHEEEECCCCCCCCCCEEEEEEECHHHHHHCCCCEE
KIEIKSPIDLPKRFEKNYGKLHSGEETARVYIRKAQYSYGWDWGARIATSGIYRDIYIES
EEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHCCCEEEEEEEC
YKDARLFGSTAFLETLDGKVNFSGYIDSCINYTKNYEVEILFNGNPVITLPVTTSVENYK
CCCCCCCCCHHHHHHCCCCEEEHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCEE
FEGSKRIENLKLWYPHDLGESYLYEVEFRLKENKEVIYGEKKKIGFRIVKVVRENDSEGE
ECCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCEEEECCCHHHHHEEEEEECCCCCCCC
SFIFEINGRKIFAKGANWIPAENILSWLKGSDYDKLLKMAKESNMNMLRVWGGGLYEDPA
EEEEEECCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCH
FYNRCDELGILVWQDFMFACAEYPDHIDWFRKLANEEVKHQVLKLRHHPSIVLWCGNNEN
HHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
NWGFEEWDYKLKVDGKNLGNRLYLEDFPKICAQEDPSRLYWPSSPYGGSRANSSEAGDRH
CCCCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
VWEIWSGWQDYKYYTWDTSKFVSEFGFQAAPDPKTIDFFAEDKEKDIFSPTMLNHNKQVE
HHHHHCCCCCCEEEEECHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHCCCCCCCC
GPERLLRFINGHYGLIDNFDSIVYLTQLNQAEAIKTGVEHWRNRKYKTAGTLYWQINDSW
CHHHHHHHHCCCCCEECCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCC
PVFSWASIDYFKRPKPLYFYTKRFYNQLLAIAKNKDEKVIISLINDGIKIKLDLEFQLWS
CEEEEECCHHHCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEEEEEE
LNGKKLMQKEYSNIKTSEDSVITVDQLNISNIDLDNSIAYLILKQNGKTIIENHELFADL
CCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEEEECHHHHHHH
RKNKLNDPKITYEKEGNNLILSCEKPAWGVNVRVNGENYSQDNFFTLFPSYQKVLNNIEG
HHCCCCCCCEEEEECCCEEEEEECCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHCCCC
EISIKSAFDYLRR
CEEHHHHHHHHCC
>Mature Secondary Structure
MKISLNGKWKVYDNEKEFEFDGNVPGTVQGDLVDLNLMPHPYVGENEKLFKRLEWKNWIY
CEEEECCEEEEECCCCCEEECCCCCCCEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
EKKFHIEEINDELRYDLVLEGVDTLSNIYLNDNFVGETEDMFIEYRFDIKKYLKIGENSL
HCEEEHHHHCCHHEEEEEEECHHHHHEEEECCCCCCCCCCEEEEEEECHHHHHHCCCCEE
KIEIKSPIDLPKRFEKNYGKLHSGEETARVYIRKAQYSYGWDWGARIATSGIYRDIYIES
EEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHCCCEEEEEEEC
YKDARLFGSTAFLETLDGKVNFSGYIDSCINYTKNYEVEILFNGNPVITLPVTTSVENYK
CCCCCCCCCHHHHHHCCCCEEEHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCEE
FEGSKRIENLKLWYPHDLGESYLYEVEFRLKENKEVIYGEKKKIGFRIVKVVRENDSEGE
ECCCCCCCEEEEECCCCCCCCEEEEEEEEEECCCEEEECCCHHHHHEEEEEECCCCCCCC
SFIFEINGRKIFAKGANWIPAENILSWLKGSDYDKLLKMAKESNMNMLRVWGGGLYEDPA
EEEEEECCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCH
FYNRCDELGILVWQDFMFACAEYPDHIDWFRKLANEEVKHQVLKLRHHPSIVLWCGNNEN
HHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
NWGFEEWDYKLKVDGKNLGNRLYLEDFPKICAQEDPSRLYWPSSPYGGSRANSSEAGDRH
CCCCCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
VWEIWSGWQDYKYYTWDTSKFVSEFGFQAAPDPKTIDFFAEDKEKDIFSPTMLNHNKQVE
HHHHHCCCCCCEEEEECHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHCCCCCCCC
GPERLLRFINGHYGLIDNFDSIVYLTQLNQAEAIKTGVEHWRNRKYKTAGTLYWQINDSW
CHHHHHHHHCCCCCEECCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCC
PVFSWASIDYFKRPKPLYFYTKRFYNQLLAIAKNKDEKVIISLINDGIKIKLDLEFQLWS
CEEEEECCHHHCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEEEEEE
LNGKKLMQKEYSNIKTSEDSVITVDQLNISNIDLDNSIAYLILKQNGKTIIENHELFADL
CCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEEEECHHHHHHH
RKNKLNDPKITYEKEGNNLILSCEKPAWGVNVRVNGENYSQDNFFTLFPSYQKVLNNIEG
HHCCCCCCCEEEEECCCEEEEEECCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHCCCC
EISIKSAFDYLRR
CEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA