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The map label for this gene is opuE [H]

Identifier: 160877524

GI number: 160877524

Start: 5226462

End: 5227958

Strand: Direct

Name: opuE [H]

Synonym: Sbal195_4423

Alternate gene names: 160877524

Gene position: 5226462-5227958 (Clockwise)

Preceding gene: 160877523

Following gene: 160877525

Centisome position: 97.74

GC content: 47.09

Gene sequence:

>1497_bases
ATGAATTACCTCGCTTACATTTCCTTAGCTATCTACTTTATCGTTATGCTCGCCATTGGCTTATTCGCTTACAAAAAATC
CACCAGCGATGTGTCAGGCTATATTCTTGGCGGCCGTAAGGTAAGTCCACAGGTCACCGCACTCTCAGCGGGTGCATCTG
ACATGAGTGGCTGGATGCTAATGGGTCTTCCCGGTGCTATGTTTTTGGTTGGTTTCGAAACCATTTACATCGCACTTGGG
CTATTGATTGGTGCTCTAATCAATTACTTGGTCGTCGCACCAAAACTTAGGGTTTATACCGAGGTGGCGGACAACGCCCT
CACTATCCCGGAGTTTTTTGCTAAACGCTTCGGCAATGCCAACGGCAGTATCCGTATTATTTCGGCCGTGATCATTGTGA
TCTTCTTTACCTTGTATACCTCAGCAGGTTTAGTGGCTGGCGGTAAATTGTTTGAGTCAGCATTTGGCCTGAACTACGAC
ATAGGTTTAGTCGTCACACTAGCGGTTGTGGTTTCTTATACCCTGTTAGGTGGATTCTTGGCAGTGAGCCTCACTGACTT
TGTCCAAGGCTGTATTATGTTTGTCGCCTTAGTCTTAGTTCCTGTCGTGGCATATCAAGAATTTACCAGCGCTGACACTA
TGTTGAACTTTGCGTATCAGTCGATTCCACACTTCACCGATGCGATGCAAAACGTCACTATGTTAGGGCTTATCTCAAGC
TTATCTTGGGGACTCGGTTACTTTGGCCAACCGCATATTATTGTGCGTTTTATGGCGATTCGTTCAGTAGCTGACATTAA
AACCGCCAGACACATAGGTATGGGCTGGATGACAGTGACCATTGTGGGTGCACTGGCAACAGGTCTTGTCGGTATTGCTT
ACGCCAATAAATTTGGTATGAAGCTGACCGATCCTGAAACCATCTTTATCGTATTTTCTGAGTTGTTGTTCCATCCGATC
ATTAGTGGCTTCTTACTGGCCGCCATTCTGGCCGCGATCATGAGCACCATTTCATCGCAGCTATTAGTGTCATCAAGCTC
GCTAACGGAAGATATCTATCGCGTGATTTCTAAGAAAGAATCTACCGAGAAAGACATGGTGAAAATGGGACGCTTTGGCG
TAGCAGGCGTAGCAATTGTCGCGAGTCTACTGGCGCTCGACCGTTCTAGCAGTGTGCTATCACTGGTGAGTAATGCATGG
GCAGGATTCGGTGCCGCCTTCGGTCCGTTAGTTTTGTTTAGCTTGTACAAAGCAAACCTAACCCACAAAGCCGCGATTGC
CGGCATAGTGTCAGGCGCTGCTACAGTGTTGTTTTGGATTTATGCCCCGGTATTGGCAGATGGACAAGCCTTAACCACAG
TGGTGTATGAAATGATCCCCGGCTTTGCCGTGAGCAGTGTGGTGATTTGGATAGTCTCGTTACTCGATACTGATCCCTGT
GCCAAAACCACTAAATTATTCCATAAGGCTGGCCGTGTTTTAGCCGAAGACAGATGA

Upstream 100 bases:

>100_bases
GCAGTTTATGTGTTTACTTGCAATATTAAGCACCTATCTCTTTGTCATTTTGAAACCGAGTATTATTCAAAATAACAAAG
CGTTAGGAGAAAAATAACCA

Downstream 100 bases:

>100_bases
GGCGCGCAGGTATGACTGACTTACCCTGGAAACGCATTGTCGTTAAGGTAGGTAGTGCCCTGATTGCACCCCACAAACAA
GGTTGCAGCAGTCACTACTT

Product: sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MNYLAYISLAIYFIVMLAIGLFAYKKSTSDVSGYILGGRKVSPQVTALSAGASDMSGWMLMGLPGAMFLVGFETIYIALG
LLIGALINYLVVAPKLRVYTEVADNALTIPEFFAKRFGNANGSIRIISAVIIVIFFTLYTSAGLVAGGKLFESAFGLNYD
IGLVVTLAVVVSYTLLGGFLAVSLTDFVQGCIMFVALVLVPVVAYQEFTSADTMLNFAYQSIPHFTDAMQNVTMLGLISS
LSWGLGYFGQPHIIVRFMAIRSVADIKTARHIGMGWMTVTIVGALATGLVGIAYANKFGMKLTDPETIFIVFSELLFHPI
ISGFLLAAILAAIMSTISSQLLVSSSSLTEDIYRVISKKESTEKDMVKMGRFGVAGVAIVASLLALDRSSSVLSLVSNAW
AGFGAAFGPLVLFSLYKANLTHKAAIAGIVSGAATVLFWIYAPVLADGQALTTVVYEMIPGFAVSSVVIWIVSLLDTDPC
AKTTKLFHKAGRVLAEDR

Sequences:

>Translated_498_residues
MNYLAYISLAIYFIVMLAIGLFAYKKSTSDVSGYILGGRKVSPQVTALSAGASDMSGWMLMGLPGAMFLVGFETIYIALG
LLIGALINYLVVAPKLRVYTEVADNALTIPEFFAKRFGNANGSIRIISAVIIVIFFTLYTSAGLVAGGKLFESAFGLNYD
IGLVVTLAVVVSYTLLGGFLAVSLTDFVQGCIMFVALVLVPVVAYQEFTSADTMLNFAYQSIPHFTDAMQNVTMLGLISS
LSWGLGYFGQPHIIVRFMAIRSVADIKTARHIGMGWMTVTIVGALATGLVGIAYANKFGMKLTDPETIFIVFSELLFHPI
ISGFLLAAILAAIMSTISSQLLVSSSSLTEDIYRVISKKESTEKDMVKMGRFGVAGVAIVASLLALDRSSSVLSLVSNAW
AGFGAAFGPLVLFSLYKANLTHKAAIAGIVSGAATVLFWIYAPVLADGQALTTVVYEMIPGFAVSSVVIWIVSLLDTDPC
AKTTKLFHKAGRVLAEDR
>Mature_498_residues
MNYLAYISLAIYFIVMLAIGLFAYKKSTSDVSGYILGGRKVSPQVTALSAGASDMSGWMLMGLPGAMFLVGFETIYIALG
LLIGALINYLVVAPKLRVYTEVADNALTIPEFFAKRFGNANGSIRIISAVIIVIFFTLYTSAGLVAGGKLFESAFGLNYD
IGLVVTLAVVVSYTLLGGFLAVSLTDFVQGCIMFVALVLVPVVAYQEFTSADTMLNFAYQSIPHFTDAMQNVTMLGLISS
LSWGLGYFGQPHIIVRFMAIRSVADIKTARHIGMGWMTVTIVGALATGLVGIAYANKFGMKLTDPETIFIVFSELLFHPI
ISGFLLAAILAAIMSTISSQLLVSSSSLTEDIYRVISKKESTEKDMVKMGRFGVAGVAIVASLLALDRSSSVLSLVSNAW
AGFGAAFGPLVLFSLYKANLTHKAAIAGIVSGAATVLFWIYAPVLADGQALTTVVYEMIPGFAVSSVVIWIVSLLDTDPC
AKTTKLFHKAGRVLAEDR

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=498, Percent_Identity=50.4016064257028, Blast_Score=494, Evalue=1e-139,
Organism=Homo sapiens, GI14140236, Length=470, Percent_Identity=22.3404255319149, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1787251, Length=488, Percent_Identity=46.7213114754098, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1790503, Length=520, Percent_Identity=25.9615384615385, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87082237, Length=447, Percent_Identity=25.503355704698, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17539284, Length=426, Percent_Identity=22.3004694835681, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24645928, Length=380, Percent_Identity=24.4736842105263, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24640370, Length=356, Percent_Identity=25, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24651739, Length=465, Percent_Identity=22.5806451612903, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53183; Mature: 53183

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]