Click here to switch to the map view.

The map label for this gene is proB1 [H]

Identifier: 160877525

GI number: 160877525

Start: 5227970

End: 5229070

Strand: Direct

Name: proB1 [H]

Synonym: Sbal195_4424

Alternate gene names: 160877525

Gene position: 5227970-5229070 (Clockwise)

Preceding gene: 160877524

Following gene: 160877526

Centisome position: 97.77

GC content: 48.5

Gene sequence:

>1101_bases
ATGACTGACTTACCCTGGAAACGCATTGTCGTTAAGGTAGGTAGTGCCCTGATTGCACCCCACAAACAAGGTTGCAGCAG
TCACTACTTACTCGGCATTGCCCAATTTATTACTTATTGTCGTGCCCAAGGGATCCAAGTTGTCTTGGTCTCTTCGGGCT
CAGTCGCCGCAGGATGGCATCACTTTGGCGAGGTTGATAAACCTAGCGTCACAGTGAAGAAAGCCATGGCAGCAGCAGGT
CAGGCCGATATGATGGCCACATGGAACAAGTTATTTGATTTCCCGACCGCACAGCTACTGTTAACCCACGGCGATTTACG
CAACCGAGATCGTTACATCAGTATTAAAGATACGATTTTCAGCCTACTCGAACATGGGCTACTGCCTATCATCAACGAGA
ACGATGCCGTGACCGCCGACAAGCTCAAAGTTGGCGATAACGATAATCTCTCTGCCATGGTAGCCGCAGCGGCCGATGCC
GATACCCTGATTATCTGTTCGGATGTGAATGGGCTTTACACACAAAACCCCCATGAAAATCCCGATGCACAGCTTATCAA
GCAGGTGACCGAGATTAACGCCGAAATATACGCCATGGCAGGCGGCGCCAGCAGCGCCGTCGGCACTGGTGGTATGCGGA
CTAAGATACAAGCCGCCAAGAAGGCAATTTCCCACGGGATCGAAACCTTTATTATCAATGGTTTTGATGCTGACTCATTC
AACCAATTGTTGAAGGGCCAAAACCCAGGAACCTTATTTACCCCCGATGAAAAACCAATGCAAGATCACTTGCACTGGAT
GACGCACACCTCGCAGGCTCAGGGCGAAGTCATTGTTGAAAATGACTTTAATATCTCCCTCGATATCCATAGCGAGCAAT
TGACCAGCGACGATGTCGTTGCCATCAATGGGGACTTTTCTGCCGGTGACACCATTTTAGTACGCAAAGGTGATGGTACT
AAATTGGCCAAGGCCAAATCCAATTACAGCAGTTGTTTACTTAGCTTTATTACGGACCAAGACGATCGCGAGTTTGCCAG
TGAATTTCAGCAAAAGACTGGACCGATCATTTCCGAGAAAAACATCGCCATTCTCGAATAA

Upstream 100 bases:

>100_bases
GGATAGTCTCGTTACTCGATACTGATCCCTGTGCCAAAACCACTAAATTATTCCATAAGGCTGGCCGTGTTTTAGCCGAA
GACAGATGAGGCGCGCAGGT

Downstream 100 bases:

>100_bases
GCATCAGCCGAATAAATATGAGCGAGTAAACATCTAAGCGGAGTAAATATGAATTTAATCAAAAACTTATCTCAAGAAGC
TGCCAAAGCGGCTCTTATTC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase 1; GK 1 [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTDLPWKRIVVKVGSALIAPHKQGCSSHYLLGIAQFITYCRAQGIQVVLVSSGSVAAGWHHFGEVDKPSVTVKKAMAAAG
QADMMATWNKLFDFPTAQLLLTHGDLRNRDRYISIKDTIFSLLEHGLLPIINENDAVTADKLKVGDNDNLSAMVAAAADA
DTLIICSDVNGLYTQNPHENPDAQLIKQVTEINAEIYAMAGGASSAVGTGGMRTKIQAAKKAISHGIETFIINGFDADSF
NQLLKGQNPGTLFTPDEKPMQDHLHWMTHTSQAQGEVIVENDFNISLDIHSEQLTSDDVVAINGDFSAGDTILVRKGDGT
KLAKAKSNYSSCLLSFITDQDDREFASEFQQKTGPIISEKNIAILE

Sequences:

>Translated_366_residues
MTDLPWKRIVVKVGSALIAPHKQGCSSHYLLGIAQFITYCRAQGIQVVLVSSGSVAAGWHHFGEVDKPSVTVKKAMAAAG
QADMMATWNKLFDFPTAQLLLTHGDLRNRDRYISIKDTIFSLLEHGLLPIINENDAVTADKLKVGDNDNLSAMVAAAADA
DTLIICSDVNGLYTQNPHENPDAQLIKQVTEINAEIYAMAGGASSAVGTGGMRTKIQAAKKAISHGIETFIINGFDADSF
NQLLKGQNPGTLFTPDEKPMQDHLHWMTHTSQAQGEVIVENDFNISLDIHSEQLTSDDVVAINGDFSAGDTILVRKGDGT
KLAKAKSNYSSCLLSFITDQDDREFASEFQQKTGPIISEKNIAILE
>Mature_365_residues
TDLPWKRIVVKVGSALIAPHKQGCSSHYLLGIAQFITYCRAQGIQVVLVSSGSVAAGWHHFGEVDKPSVTVKKAMAAAGQ
ADMMATWNKLFDFPTAQLLLTHGDLRNRDRYISIKDTIFSLLEHGLLPIINENDAVTADKLKVGDNDNLSAMVAAAADAD
TLIICSDVNGLYTQNPHENPDAQLIKQVTEINAEIYAMAGGASSAVGTGGMRTKIQAAKKAISHGIETFIINGFDADSFN
QLLKGQNPGTLFTPDEKPMQDHLHWMTHTSQAQGEVIVENDFNISLDIHSEQLTSDDVVAINGDFSAGDTILVRKGDGTK
LAKAKSNYSSCLLSFITDQDDREFASEFQQKTGPIISEKNIAILE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=291, Percent_Identity=30.5841924398625, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI21361368, Length=293, Percent_Identity=30.3754266211604, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1786437, Length=357, Percent_Identity=33.3333333333333, Blast_Score=218, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=33.7078651685393, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=33.7078651685393, Blast_Score=134, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6321822, Length=231, Percent_Identity=39.3939393939394, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6320506, Length=231, Percent_Identity=37.6623376623377, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21357643, Length=284, Percent_Identity=30.9859154929577, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39644; Mature: 39513

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPWKRIVVKVGSALIAPHKQGCSSHYLLGIAQFITYCRAQGIQVVLVSSGSVAAGWH
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCEEECHH
HFGEVDKPSVTVKKAMAAAGQADMMATWNKLFDFPTAQLLLTHGDLRNRDRYISIKDTIF
HCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHH
SLLEHGLLPIINENDAVTADKLKVGDNDNLSAMVAAAADADTLIICSDVNGLYTQNPHEN
HHHHCCCCEEECCCCCEEEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCC
PDAQLIKQVTEINAEIYAMAGGASSAVGTGGMRTKIQAAKKAISHGIETFIINGFDADSF
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHH
NQLLKGQNPGTLFTPDEKPMQDHLHWMTHTSQAQGEVIVENDFNISLDIHSEQLTSDDVV
HHHHCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCEEEECCCEEEEEECHHHCCCCCEE
AINGDFSAGDTILVRKGDGTKLAKAKSNYSSCLLSFITDQDDREFASEFQQKTGPIISEK
EEECCCCCCCEEEEECCCCCEEHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCC
NIAILE
CEEEEC
>Mature Secondary Structure 
TDLPWKRIVVKVGSALIAPHKQGCSSHYLLGIAQFITYCRAQGIQVVLVSSGSVAAGWH
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCEEECHH
HFGEVDKPSVTVKKAMAAAGQADMMATWNKLFDFPTAQLLLTHGDLRNRDRYISIKDTIF
HCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHH
SLLEHGLLPIINENDAVTADKLKVGDNDNLSAMVAAAADADTLIICSDVNGLYTQNPHEN
HHHHCCCCEEECCCCCEEEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCC
PDAQLIKQVTEINAEIYAMAGGASSAVGTGGMRTKIQAAKKAISHGIETFIINGFDADSF
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHH
NQLLKGQNPGTLFTPDEKPMQDHLHWMTHTSQAQGEVIVENDFNISLDIHSEQLTSDDVV
HHHHCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCEEEECCCEEEEEECHHHCCCCCEE
AINGDFSAGDTILVRKGDGTKLAKAKSNYSSCLLSFITDQDDREFASEFQQKTGPIISEK
EEECCCCCCCEEEEECCCCCEEHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCC
NIAILE
CEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA