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The map label for this gene is rlmI

Identifier: 160876621

GI number: 160876621

Start: 4131037

End: 4132227

Strand: Reverse

Name: rlmI

Synonym: Sbal195_3515

Alternate gene names: 160876621

Gene position: 4132227-4131037 (Counterclockwise)

Preceding gene: 160876625

Following gene: 160876619

Centisome position: 77.28

GC content: 48.53

Gene sequence:

>1191_bases
ATGGCTATCAGAATCAAACTTAAACCCGGCCGCGAGAAGTCGCTCGAGCGTCGCCATCCTTGGGTATTTTCCAATGCTAT
TCATAACATTAAGGGAAAACCTGAAGCGGGTGAGACGGTCGATGTGGTCGCTCATGATGGTCATTGGTTAGGTCGTGGTG
CTTGGTCGCCTGAGTCGCAAATCCAAGTGCGGATTTGGACTTTCGATCGAGAAGAAGAAATTGATCGCGAGTTTTTTGCC
AGACGTTTACAGCGGGCACAAATTGGCCGTAATGATTTAATTCGTGAGCAAGGCTTAACGGGTTATCGTTTAGTGGCCGC
CGAGTCCGATGGTTTGCCAGGGATCACCATCGACAGATATGCCAATGTGTTGGTGTGCCAGTTACTGAGCACTGGGGCTG
ATTTATGGCGTGATACCTTAGTTGAGTTATTGGCTGAGCAATATCCTGATTGTGCTATCTATGAACGTTCCGATGTGGAT
TCCCGCAAGAAAGAAGGCCTGCTGCCAGTTACCGGTTTACTGCACGGCACGCTGCCTGAAATGCCGGTGATTATCGAAGA
AAACGGCATCAAGATCGCCGTCGATGTGATTAAAGGTCATAAGACAGGTTTCTATCTCGATCAACGTGATAATCGCGCCA
TTGCCGCCCGTTTTGTGAAAGATAAATCGGTATTGAACTGTTTCTGCTACACAGGCACCTTTGGTTTGTACGCCGCCAAA
GCAGGCGCTGCCAGCATAGAAAACGTCGATGTGTCTTCTTTAGCATTAGCGACGGCGCGTCTAAATATGCAAGTGAACGG
TTTGAGTGATGACAATGTGCATTACAACGAAGCCGATGTGTTCAAGTTGCTGCGTCTATACCGTGATGAAGGCAAAACCT
TCGATGTGATCGTGCTCGACCCGCCTAAGTTTGCCGATAACAAGGCACAGTTAAACGGGGCATGCCGTGGTTATAAAGAC
ATCAACATGATTGCATTGCAGTTGTTGAATCCTGGCGGGGTGTTATTGACCTTCTCTTGCTCGGGATTAATGCCTGCGGA
TCTGTTCCAAAAAATCGTGGCCGATGCGGCGTTGGACGCAAAACGCGAAATCCAGTTCATCGAACGCTTAAGCCAAGCAA
GTGATCACCCAATAGGTAGCGCCTTCCCAGAAGGATTCTACTTAAAAGGCTTAGTCGCCAGAGCTTGGTAA

Upstream 100 bases:

>100_bases
CGTTCGGTACTCGCACTCAGGTGACATTGGGTATATCATCCGTTTCCCATTTTTTAGACAGCAGCCCTTTTTTAGTGGCG
GCACTATTTTTAAGGCAACT

Downstream 100 bases:

>100_bases
GAGCCGCCAGAGCTTGGTAGATAGTCGCTAGAGCTTAAGCCAAGCGCTTGATATAAGCTGCCTGCGATGTGGGTTTAGTT
AGAGAATAGGCCTTAGTTAG

Product: PUA domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQIQVRIWTFDREEEIDREFFA
RRLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRYANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVD
SRKKEGLLPVTGLLHGTLPEMPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAK
AGAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRLYRDEGKTFDVIVLDPPKFADNKAQLNGACRGYKD
INMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDAKREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW

Sequences:

>Translated_396_residues
MAIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQIQVRIWTFDREEEIDREFFA
RRLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRYANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVD
SRKKEGLLPVTGLLHGTLPEMPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAK
AGAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRLYRDEGKTFDVIVLDPPKFADNKAQLNGACRGYKD
INMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDAKREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW
>Mature_395_residues
AIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQIQVRIWTFDREEEIDREFFAR
RLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRYANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVDS
RKKEGLLPVTGLLHGTLPEMPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAKA
GAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRLYRDEGKTFDVIVLDPPKFADNKAQLNGACRGYKDI
NMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDAKREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Escherichia coli, GI145693111, Length=394, Percent_Identity=58.1218274111675, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1787181, Length=250, Percent_Identity=28.4, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMI_SHEB5 (A3D7R1)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001051643.1
- ProteinModelPortal:   A3D7R1
- SMR:   A3D7R1
- STRING:   A3D7R1
- GeneID:   4841517
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_3294
- NMPDR:   fig|325240.3.peg.1178
- eggNOG:   COG1092
- HOGENOM:   HBG411897
- OMA:   RAAYSPH
- PhylomeDB:   A3D7R1
- ProtClustDB:   CLSK2462133
- GO:   GO:0005737
- HAMAP:   MF_01857
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614
- SMART:   SM00359

Pfam domain/function: PF10672 Methyltrans_SAM; SSF88697 PUA-like

EC number: =2.1.1.191

Molecular weight: Translated: 44144; Mature: 44013

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50890 PUA; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQ
CEEEEEECCCCCHHHHHCCCEEEHHHHHCCCCCCCCCCEEEEEEECCEEECCCCCCCCCC
IQVRIWTFDREEEIDREFFARRLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRY
EEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCCCEEHHHH
ANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVDSRKKEGLLPVTGLLHGTLPE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCHHHHHHCCCCC
MPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAK
CCEEEECCCCEEEEEEEECCCCCEEEECCCCCEEEHHHHHCCCCEEEEEEECCCHHHHHC
AGAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRLYRDEGKTFDVIVLD
CCCCCCCCCCHHHHEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEC
PPKFADNKAQLNGACRGYKDINMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDA
CCCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCH
KREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCC
>Mature Secondary Structure 
AIRIKLKPGREKSLERRHPWVFSNAIHNIKGKPEAGETVDVVAHDGHWLGRGAWSPESQ
EEEEEECCCCCHHHHHCCCEEEHHHHHCCCCCCCCCCEEEEEEECCEEECCCCCCCCCC
IQVRIWTFDREEEIDREFFARRLQRAQIGRNDLIREQGLTGYRLVAAESDGLPGITIDRY
EEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCCCEEHHHH
ANVLVCQLLSTGADLWRDTLVELLAEQYPDCAIYERSDVDSRKKEGLLPVTGLLHGTLPE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHCCCCCHHHHHHCCCCC
MPVIIEENGIKIAVDVIKGHKTGFYLDQRDNRAIAARFVKDKSVLNCFCYTGTFGLYAAK
CCEEEECCCCEEEEEEEECCCCCEEEECCCCCEEEHHHHHCCCCEEEEEEECCCHHHHHC
AGAASIENVDVSSLALATARLNMQVNGLSDDNVHYNEADVFKLLRLYRDEGKTFDVIVLD
CCCCCCCCCCHHHHEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEC
PPKFADNKAQLNGACRGYKDINMIALQLLNPGGVLLTFSCSGLMPADLFQKIVADAALDA
CCCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCH
KREIQFIERLSQASDHPIGSAFPEGFYLKGLVARAW
HHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA