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The map label for this gene is yafC [C]

Identifier: 160875639

GI number: 160875639

Start: 2986671

End: 2987549

Strand: Direct

Name: yafC [C]

Synonym: Sbal195_2527

Alternate gene names: 160875639

Gene position: 2986671-2987549 (Clockwise)

Preceding gene: 160875636

Following gene: 160875641

Centisome position: 55.85

GC content: 49.49

Gene sequence:

>879_bases
ATGTACCTCTGGGATGGCATTTCTGAGTTTGTCGCAGTGGCCGAAGCCGAAAGCTTTACCTTAGCGGCGCAGCGACTGAA
TATCTCCACCGCCCACGTGAGCCGCCAAGTCAGCGCCTTAGAGAATCGCCTCGGCACTAAGCTGTTTTATCGCACCACCC
GCAAAGTGTCGCTAACCGAAGAAGGCACGATTTACTATCGCCATTGCCGCCAATTACAAACCGGCTTAGAAGAAGCTGAG
CGCGCCATCTCAAACCTAAAGGACTCACCCCAAGGCTTAGTCAAACTCACGGCTCCCGTGGCCTATGGCGAGAAATTTAT
CATGCCGTTACTCAATGATTTTATGGTGCAATATCCCAGCGTTGAATTTAGCGTCGACTTGACCAATCGCACCTTGGACT
TGATTGAAGGCGGTTATGATCTGGCCATTCGCCTCGGAAAACTCGCCGATTCGAGCCTTATGGCCAAACCGCTCAGCAGC
CGTACTCACTATGTCGCCGCCTCACCAAGTTATGTGGAAAAATACGGCCAGCCCCATACTTTATCTGAACTTAGCCAGCA
CAATTGTTTGATCGGTAATCATAATTACTGGCGCTTTGTGGAAAATGGCCGCGAGCGTAATATCAAAGTCCGTGGCAATC
TCAATTGTAACAGCGGCTACGCATTAAGGGATGCGGCACTAAAAGGCATAGGGATAGTTCAACTGCCTGATTATTATATT
GAAGATGATATAAATGCAGGACATTTACTGTCCTTACTCGACGGTCACCGCGAAGCCAAAGAAGGCATTTGGGCGCTCTA
TCCGCAAAACCGCCATTTATCGGCCAAGATCCGCGTATTGGTCGATTACCTCGCCGAAAAACTCATCGAACAGGACTAA

Upstream 100 bases:

>100_bases
ATGCCAATTGCACTGATGTTACTATATAGGAAGATTGTATTTGTTTCTCAGTGAGAGACAATCTAGTATTCAAGCAATTC
ATTTTTACTGAGTAACAACA

Downstream 100 bases:

>100_bases
TCTTTTTCTGAACAGCCGGTTGTTGAGCAGTATTTTGTGGAGCAGTCTGTTGCTAAAAAATCTATTGCTGAGAGGTTTAT
TGTGGAGCCGTCTGTTGTTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MYLWDGISEFVAVAEAESFTLAAQRLNISTAHVSRQVSALENRLGTKLFYRTTRKVSLTEEGTIYYRHCRQLQTGLEEAE
RAISNLKDSPQGLVKLTAPVAYGEKFIMPLLNDFMVQYPSVEFSVDLTNRTLDLIEGGYDLAIRLGKLADSSLMAKPLSS
RTHYVAASPSYVEKYGQPHTLSELSQHNCLIGNHNYWRFVENGRERNIKVRGNLNCNSGYALRDAALKGIGIVQLPDYYI
EDDINAGHLLSLLDGHREAKEGIWALYPQNRHLSAKIRVLVDYLAEKLIEQD

Sequences:

>Translated_292_residues
MYLWDGISEFVAVAEAESFTLAAQRLNISTAHVSRQVSALENRLGTKLFYRTTRKVSLTEEGTIYYRHCRQLQTGLEEAE
RAISNLKDSPQGLVKLTAPVAYGEKFIMPLLNDFMVQYPSVEFSVDLTNRTLDLIEGGYDLAIRLGKLADSSLMAKPLSS
RTHYVAASPSYVEKYGQPHTLSELSQHNCLIGNHNYWRFVENGRERNIKVRGNLNCNSGYALRDAALKGIGIVQLPDYYI
EDDINAGHLLSLLDGHREAKEGIWALYPQNRHLSAKIRVLVDYLAEKLIEQD
>Mature_292_residues
MYLWDGISEFVAVAEAESFTLAAQRLNISTAHVSRQVSALENRLGTKLFYRTTRKVSLTEEGTIYYRHCRQLQTGLEEAE
RAISNLKDSPQGLVKLTAPVAYGEKFIMPLLNDFMVQYPSVEFSVDLTNRTLDLIEGGYDLAIRLGKLADSSLMAKPLSS
RTHYVAASPSYVEKYGQPHTLSELSQHNCLIGNHNYWRFVENGRERNIKVRGNLNCNSGYALRDAALKGIGIVQLPDYYI
EDDINAGHLLSLLDGHREAKEGIWALYPQNRHLSAKIRVLVDYLAEKLIEQD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=282, Percent_Identity=37.2340425531915, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=32.2916666666667, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1789440, Length=281, Percent_Identity=30.2491103202847, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=29.616724738676, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=29.1666666666667, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=29.1525423728814, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1787589, Length=285, Percent_Identity=28.0701754385965, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786448, Length=281, Percent_Identity=24.1992882562278, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790011, Length=304, Percent_Identity=25, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788706, Length=189, Percent_Identity=30.1587301587302, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789173, Length=249, Percent_Identity=26.5060240963855, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=155, Percent_Identity=30.9677419354839, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788297, Length=113, Percent_Identity=33.6283185840708, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790783, Length=266, Percent_Identity=25.9398496240602, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=166, Percent_Identity=28.3132530120482, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787806, Length=271, Percent_Identity=24.3542435424354, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787879, Length=141, Percent_Identity=32.6241134751773, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32967; Mature: 32967

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLWDGISEFVAVAEAESFTLAAQRLNISTAHVSRQVSALENRLGTKLFYRTTRKVSLTE
CCCCCCHHHHHHHHCCCHHEEHHHHCCCHHHHHHHHHHHHHHHHCCHHEEEECCEEEECC
EGTIYYRHCRQLQTGLEEAERAISNLKDSPQGLVKLTAPVAYGEKFIMPLLNDFMVQYPS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCHHHHHHHHHHHHHHCCC
VEFSVDLTNRTLDLIEGGYDLAIRLGKLADSSLMAKPLSSRTHYVAASPSYVEKYGQPHT
EEEEEEECCCCHHHHCCCCEEEEEECHHCCCHHHHCCCCCCCEEEECCCHHHHHCCCCCC
LSELSQHNCLIGNHNYWRFVENGRERNIKVRGNLNCNSGYALRDAALKGIGIVQLPDYYI
HHHHHHCCCEECCCCEEEEECCCCCCEEEEEECCCCCCCCEEHHHHHCCCEEEECCCEEE
EDDINAGHLLSLLDGHREAKEGIWALYPQNRHLSAKIRVLVDYLAEKLIEQD
CCCCCHHHHHHHHHCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYLWDGISEFVAVAEAESFTLAAQRLNISTAHVSRQVSALENRLGTKLFYRTTRKVSLTE
CCCCCCHHHHHHHHCCCHHEEHHHHCCCHHHHHHHHHHHHHHHHCCHHEEEECCEEEECC
EGTIYYRHCRQLQTGLEEAERAISNLKDSPQGLVKLTAPVAYGEKFIMPLLNDFMVQYPS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHCCHHHHHHHHHHHHHHCCC
VEFSVDLTNRTLDLIEGGYDLAIRLGKLADSSLMAKPLSSRTHYVAASPSYVEKYGQPHT
EEEEEEECCCCHHHHCCCCEEEEEECHHCCCHHHHCCCCCCCEEEECCCHHHHHCCCCCC
LSELSQHNCLIGNHNYWRFVENGRERNIKVRGNLNCNSGYALRDAALKGIGIVQLPDYYI
HHHHHHCCCEECCCCEEEEECCCCCCEEEEEECCCCCCCCEEHHHHHCCCEEEECCCEEE
EDDINAGHLLSLLDGHREAKEGIWALYPQNRHLSAKIRVLVDYLAEKLIEQD
CCCCCHHHHHHHHHCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]