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The map label for this gene is nth [H]

Identifier: 160875219

GI number: 160875219

Start: 2515413

End: 2516054

Strand: Reverse

Name: nth [H]

Synonym: Sbal195_2106

Alternate gene names: 160875219

Gene position: 2516054-2515413 (Counterclockwise)

Preceding gene: 160875220

Following gene: 160875217

Centisome position: 47.05

GC content: 47.04

Gene sequence:

>642_bases
ATGAATCAAGAAAAACGCATCCAGATCCTCACCCGTCTTCGCGATAACAATCCGAAACCTGAAACCGAACTCAATTTTTC
CAGTCCTTTCGAACTCTTAGTCGCGGTGACCTTATCCGCCCAAGCGACGGATGTGAGCGTTAACAAGGCAACGGACAAAC
TCTTTCCTGTGGCGAACACTGCCCATAGCATTTATGCCCTTGGCGTCGATGGTTTAAAACAATACATAAAAACCATAGGC
TTATATAACAACAAAGCCGTCAATGTGATTAAAGCCTGTGAGATCTTGATTGAGAAATACAATGGCGAAGTGCCAGAAAA
CCGCGAAGCCCTAGAGTCTCTGCCAGGCGTTGGCCGCAAAACGGCTAACGTGGTACTCAATACTGCATTTGGTTGGCCAA
CGATCGCCGTCGACACCCATATTTTCCGCCTCGCTAATCGCACTAAATTTGCGCCCGGCAAGAACGTGGTCGATGTGGAA
GATAAAATGCTGAAAGTCGTGCCTGCAGAATTTAAAGTCGATGTGCATCACTGGTTTATCTTGCACGGGCGCTACACTTG
CCTCGCGCGCAAACCTCGCTGCGGCAGCTGTATCATTGAAGACTTGTGCGAATTTAAAGAAAAAATCTATCCTGAAGAAT
AA

Upstream 100 bases:

>100_bases
ACATCAGCAGGTCTAGGTTTCAAGCTCGTTCAATCATCATTAATACCCTATTGATATTGATAATGACGAGTTGAACCCAA
CAACCGCGCAGGAATCGTAC

Downstream 100 bases:

>100_bases
ACCCAAGCAAGTTAGCCTTGATTTTTCTTCTCGAGCAAACTGACTAACTCTTCATATTTCGGCCCTTGCCCAAATTGCGG
CAAGGCCACTGCAAAGACCA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNQEKRIQILTRLRDNNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAHSIYALGVDGLKQYIKTIG
LYNNKAVNVIKACEILIEKYNGEVPENREALESLPGVGRKTANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVDVE
DKMLKVVPAEFKVDVHHWFILHGRYTCLARKPRCGSCIIEDLCEFKEKIYPEE

Sequences:

>Translated_213_residues
MNQEKRIQILTRLRDNNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAHSIYALGVDGLKQYIKTIG
LYNNKAVNVIKACEILIEKYNGEVPENREALESLPGVGRKTANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVDVE
DKMLKVVPAEFKVDVHHWFILHGRYTCLARKPRCGSCIIEDLCEFKEKIYPEE
>Mature_213_residues
MNQEKRIQILTRLRDNNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANTAHSIYALGVDGLKQYIKTIG
LYNNKAVNVIKACEILIEKYNGEVPENREALESLPGVGRKTANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVDVE
DKMLKVVPAEFKVDVHHWFILHGRYTCLARKPRCGSCIIEDLCEFKEKIYPEE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=183, Percent_Identity=28.4153005464481, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=79.4258373205742, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=31.0734463276836, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=25.668449197861, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550361, Length=187, Percent_Identity=33.1550802139037, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23970; Mature: 23970

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2 ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQEKRIQILTRLRDNNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEECCHHHHHHCCCCCC
AHSIYALGVDGLKQYIKTIGLYNNKAVNVIKACEILIEKYNGEVPENREALESLPGVGRK
CCEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVDVEDKMLKVVPAEFKVDVHHWFI
HHHEEEECCCCCCEEEHHHHHHHHHCCCCCCCCCCEECHHHHHHHHCCCCEEEEEEEEEE
LHGRYTCLARKPRCGSCIIEDLCEFKEKIYPEE
EECCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNQEKRIQILTRLRDNNPKPETELNFSSPFELLVAVTLSAQATDVSVNKATDKLFPVANT
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEECCHHHHHHCCCCCC
AHSIYALGVDGLKQYIKTIGLYNNKAVNVIKACEILIEKYNGEVPENREALESLPGVGRK
CCEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
TANVVLNTAFGWPTIAVDTHIFRLANRTKFAPGKNVVDVEDKMLKVVPAEFKVDVHHWFI
HHHEEEECCCCCCEEEHHHHHHHHHCCCCCCCCCCEECHHHHHHHHCCCCEEEEEEEEEE
LHGRYTCLARKPRCGSCIIEDLCEFKEKIYPEE
EECCEEEEECCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]