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The map label for this gene is gloA [H]

Identifier: 160875217

GI number: 160875217

Start: 2514271

End: 2514681

Strand: Reverse

Name: gloA [H]

Synonym: Sbal195_2104

Alternate gene names: 160875217

Gene position: 2514681-2514271 (Counterclockwise)

Preceding gene: 160875219

Following gene: 160875214

Centisome position: 47.03

GC content: 47.2

Gene sequence:

>411_bases
ATGTCGCAACTATTACACACTATGATCCGCGTCGGTAACTTAGAACGCAGTATTGCTTTTTATACTGAAGTGCTCGGCAT
GAAACTCCTTCGCACCTCTGAAAACCCAGAATACAAATATTCTTTAGCTTTCGTAGGTTACGGTGAAGAGTCCACAGGCC
AAGCCGTGGTTGAACTGACCTATAACTGGGGCACAGACAAATACGATTTAGGCACAGGCTTTGGCCACTTAGCCATTGGC
GATGAAGATATTTACGCCCGTTGTGCTGCTATTGCTGCAGCCGGTGGCAAAGTAACACGCGCCCCAGGTCCAGTCGCAGG
CGGCACCACTGAGATTGCCTTTGTTGAAGATCCAGACGGTTATAAAATTGAGTTCATCCAAATGAAATCGGCGACTCAAG
GTTTAGGTTAA

Upstream 100 bases:

>100_bases
TTGGTCAAGTTCGATAAAATCCCCATAAATAACAATTGTGGTCAAACGCAGAGACATTTTTGATATACTCCTCACCGTAA
TCTATTTATAGAGAGGACCT

Downstream 100 bases:

>100_bases
ATCTTGCCACCTGAGCAAGAAGATTAACTGAGCTAGATTAAGTGAGCAGAACAGTTCGCTAAATGAGAAATGCCGAATCT
TGATTCGGCATTTTTGTTTT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENPEYKYSLAFVGYGEESTGQAVVELTYNWGTDKYDLGTGFGHLAIG
DEDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIEFIQMKSATQGLG

Sequences:

>Translated_136_residues
MSQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENPEYKYSLAFVGYGEESTGQAVVELTYNWGTDKYDLGTGFGHLAIG
DEDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIEFIQMKSATQGLG
>Mature_135_residues
SQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENPEYKYSLAFVGYGEESTGQAVVELTYNWGTDKYDLGTGFGHLAIGD
EDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDGYKIEFIQMKSATQGLG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=147, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=69.4029850746269, Blast_Score=190, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6323639, Length=140, Percent_Identity=42.8571428571429, Blast_Score=105, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129743, Length=149, Percent_Identity=35.5704697986577, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19920404, Length=139, Percent_Identity=31.6546762589928, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14652; Mature: 14521

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENPEYKYSLAFVGYGEESTGQAVVELT
CHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCCEEEEEE
YNWGTDKYDLGTGFGHLAIGDEDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDG
ECCCCCEEECCCCCEEEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIEFIQMKSATQGLG
CEEEEEEECCCCCCCC
>Mature Secondary Structure 
SQLLHTMIRVGNLERSIAFYTEVLGMKLLRTSENPEYKYSLAFVGYGEESTGQAVVELT
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCCCCCEEEEEE
YNWGTDKYDLGTGFGHLAIGDEDIYARCAAIAAAGGKVTRAPGPVAGGTTEIAFVEDPDG
ECCCCCEEECCCCCEEEEECCHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCC
YKIEFIQMKSATQGLG
CEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739; 8021208 [H]