Click here to switch to the map view.

The map label for this gene is 160874441

Identifier: 160874441

GI number: 160874441

Start: 1570582

End: 1572252

Strand: Direct

Name: 160874441

Synonym: Sbal195_1322

Alternate gene names: NA

Gene position: 1570582-1572252 (Clockwise)

Preceding gene: 160874440

Following gene: 160874443

Centisome position: 29.37

GC content: 48.77

Gene sequence:

>1671_bases
ATGGATAGACGTCAATTTCTAGCAGGCTCAGCCGCCCTAATGACGACCGCAGTTTGGGCAAGTTCAGTTGATAAATTACT
GGCGCTGGATAACGCACAAGCGGTCGCCCGTGAGCCATGGGCCATAGGTTTTAACGGGGCGGAAGGCGACTATCAGCCGC
TCACTATGCAGGTTTCGGGCGAATGGCCTGCACAATTGAGCGGCAATCTATATCGTAATGGGCCTGCAAAAATGTCTCGG
GCAGGTAAGGCATATCAACATTGGTTCGATGGCGATGGCATGGTGCAGCAGTTTAGTGTGGTTAATGGGCAAATTCAGCA
TCAGGCTAAGTTTATCCAAACAAAGAAGTTTAAAGTGGAGGAGGCCGCTGGCGCATTTAAGTACACCACCGCAGGCACAG
TGATCCCTAATGCTTTACCCATTCGCAATAACGATGACTTGAATGTGGCTAACACCAGTTTATTACCGCGCCACGGCGAG
TTGCTCGCTTTGTGGGAAGCGGGTTCACCCTATCGACTCGATGCGCAGACCTTAACCACGCTCGGTGTTAAAAGCTTTGG
CGACAAGTTTAAGGGCTTACCCTTTTCCGCCCATCCACTCGATGATGGACAAGGTGGATTGTGGAATTTTGGCGTCTGGT
ATGTCGGCGGCGAGAAGCAATTACTTGTGTATCAAGTGGCAGCCGATGACAGCATTGCCAGAATGGAAGTGATTGAATTA
CCACAGGCGAGTTATTTACATGCCTTCGCGCAGAGTGAAAATAAGCTGGTGTTTTATATTTCATCTTGCGTGTATGAAGA
GGGCGAAACTTATATCGATGCTTTTAAATGGCGCCCAGAATTGCCATCGCAATTACTGGTTATCGACAAAGCCGACTTTA
AAGCGCGCCAGTTACACCCGCTGCCTGCAGGATTTGTGTTTCACTTTGGCCAAGCCATTGAGAAGAATGGCGAACTATCA
GTGCAACTTTGCCTCTATCCCAATTCGGCAATTTTAACTCAAGGGATGAAGGGGTTACTCACGGGCGCCCGTAAAACAAA
AACACCCCATGCCGAGTTAGTGACGATCACAGTACCGCTAACCGCACCACTTACAGCTACTAGCAAAACGCAGCTTGCAG
AGACCTTGCCTTCTTCTGCACGAATTAACCGCAGCGGCGTGATGATGGAGTTCCCCCAGTTTGCGCAGCAAACTATGAGT
AACAAAGAGTCTGCACAGCAAACGAGCAATGCCGAAAAGCTTGCAGAGAAAACGGGTGGGGCTTCGGAATTTGTCCCGTT
ATTTGGCGTTGGAGCAAGATCAGACTCTGCTGCTAATTCTGGTGCTAATTCTAACTTTAAATATGAAGCCGAATCTGAAT
CAGGACTGAGTAATACTCTGTATTGTATTCATAATGCAAAAGACAGCAGCCAGCACGGTGTGGATACACTTGGCGACGCG
AGCTATAGCGCGTTTTATCTTGGCAAAGGCAAGATTGCCGAAGAGCCGCTCTATATCCCCGCCACTGAGCAGCATGAAGC
CTATTTGTTGATGACTTGGCTCGATTACCACAATGCACAATCTGGCTTGTCTTTGTTTCGTGCCAGCGACATCAGTGCGG
GACCCATTGCTTCGGCTCATATGAACAGGGTATTGCCGTTAGGGTTTCACGGTTGTTTCATTAACGCTTAA

Upstream 100 bases:

>100_bases
ACGCCGGCAGTTTTGGACCTGCGAGCTTTAAAGACGCCAGCTTTAGTTTGCAGGCCGATGCGCAAATCACTATACATATT
CGCTAAGGGAGCGCTGCACC

Downstream 100 bases:

>100_bases
TATGTTGACCTATAAAAAAACGCAGCCAAGGGCTGCGTTTTATTTCAGGCGAAAGCGGTGCTTAGTCTTCAATTTCCGCA
TTGTGATAGACGTTTTGTAC

Product: carotenoid oxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MDRRQFLAGSAALMTTAVWASSVDKLLALDNAQAVAREPWAIGFNGAEGDYQPLTMQVSGEWPAQLSGNLYRNGPAKMSR
AGKAYQHWFDGDGMVQQFSVVNGQIQHQAKFIQTKKFKVEEAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGE
LLALWEAGSPYRLDAQTLTTLGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHPLPAGFVFHFGQAIEKNGELS
VQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPLTAPLTATSKTQLAETLPSSARINRSGVMMEFPQFAQQTMS
NKESAQQTSNAEKLAEKTGGASEFVPLFGVGARSDSAANSGANSNFKYEAESESGLSNTLYCIHNAKDSSQHGVDTLGDA
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAHMNRVLPLGFHGCFINA

Sequences:

>Translated_556_residues
MDRRQFLAGSAALMTTAVWASSVDKLLALDNAQAVAREPWAIGFNGAEGDYQPLTMQVSGEWPAQLSGNLYRNGPAKMSR
AGKAYQHWFDGDGMVQQFSVVNGQIQHQAKFIQTKKFKVEEAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGE
LLALWEAGSPYRLDAQTLTTLGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHPLPAGFVFHFGQAIEKNGELS
VQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPLTAPLTATSKTQLAETLPSSARINRSGVMMEFPQFAQQTMS
NKESAQQTSNAEKLAEKTGGASEFVPLFGVGARSDSAANSGANSNFKYEAESESGLSNTLYCIHNAKDSSQHGVDTLGDA
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAHMNRVLPLGFHGCFINA
>Mature_556_residues
MDRRQFLAGSAALMTTAVWASSVDKLLALDNAQAVAREPWAIGFNGAEGDYQPLTMQVSGEWPAQLSGNLYRNGPAKMSR
AGKAYQHWFDGDGMVQQFSVVNGQIQHQAKFIQTKKFKVEEAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGE
LLALWEAGSPYRLDAQTLTTLGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHPLPAGFVFHFGQAIEKNGELS
VQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPLTAPLTATSKTQLAETLPSSARINRSGVMMEFPQFAQQTMS
NKESAQQTSNAEKLAEKTGGASEFVPLFGVGARSDSAANSGANSNFKYEAESESGLSNTLYCIHNAKDSSQHGVDTLGDA
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAHMNRVLPLGFHGCFINA

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI82617628, Length=359, Percent_Identity=23.1197771587744, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI82617624, Length=359, Percent_Identity=23.1197771587744, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI4506591, Length=382, Percent_Identity=23.5602094240838, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI74027270, Length=379, Percent_Identity=21.1081794195251, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71998242, Length=419, Percent_Identity=23.3890214797136, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI32564756, Length=427, Percent_Identity=23.4192037470726, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646669, Length=567, Percent_Identity=19.7530864197531, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 60516; Mature: 60516

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRQFLAGSAALMTTAVWASSVDKLLALDNAQAVAREPWAIGFNGAEGDYQPLTMQVSG
CCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEECCCCCCCCCEEEEEECC
EWPAQLSGNLYRNGPAKMSRAGKAYQHWFDGDGMVQQFSVVNGQIQHQAKFIQTKKFKVE
CCCCCCCCCEEECCCHHHHHCCHHHHHEECCCCHHHHHHHHCCHHHHHHHHHHHHCEEEH
EAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGELLALWEAGSPYRLDAQTLTT
HHCCCEEEEECCCCCCCEECCCCCCCCCEECCCCCCCCCCEEEEEECCCCEEECHHHHHH
LGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCHHEEHEECC
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHP
CCHHHHHHHHCCCCCEEEEEEEEHHCCCCCEEEHEECCCCCCCEEEEEECCCCCHHHCCC
LPAGFVFHFGQAIEKNGELSVQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPL
CCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCHHHHHCCCCCCCCCCCEEEEEEEEC
TAPLTATSKTQLAETLPSSARINRSGVMMEFPQFAQQTMSNKESAQQTSNAEKLAEKTGG
CCCCCCCCHHHHHHHCCCCCEECCCCCEEECHHHHHHHHCCHHHHHHHCCHHHHHHHCCC
ASEFVPLFGVGARSDSAANSGANSNFKYEAESESGLSNTLYCIHNAKDSSQHGVDTLGDA
CCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAH
CCEEEEECCCCCCCCCEECCCCCCCCEEEEEEEECCCCCHHHHHHEEECCCCCCCCCHHH
MNRVLPLGFHGCFINA
HCCCCEEEECCEEECC
>Mature Secondary Structure
MDRRQFLAGSAALMTTAVWASSVDKLLALDNAQAVAREPWAIGFNGAEGDYQPLTMQVSG
CCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEECCCCCCCCCEEEEEECC
EWPAQLSGNLYRNGPAKMSRAGKAYQHWFDGDGMVQQFSVVNGQIQHQAKFIQTKKFKVE
CCCCCCCCCEEECCCHHHHHCCHHHHHEECCCCHHHHHHHHCCHHHHHHHHHHHHCEEEH
EAAGAFKYTTAGTVIPNALPIRNNDDLNVANTSLLPRHGELLALWEAGSPYRLDAQTLTT
HHCCCEEEEECCCCCCCEECCCCCCCCCEECCCCCCCCCCEEEEEECCCCEEECHHHHHH
LGVKSFGDKFKGLPFSAHPLDDGQGGLWNFGVWYVGGEKQLLVYQVAADDSIARMEVIEL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCHHEEHEECC
PQASYLHAFAQSENKLVFYISSCVYEEGETYIDAFKWRPELPSQLLVIDKADFKARQLHP
CCHHHHHHHHCCCCCEEEEEEEEHHCCCCCEEEHEECCCCCCCEEEEEECCCCCHHHCCC
LPAGFVFHFGQAIEKNGELSVQLCLYPNSAILTQGMKGLLTGARKTKTPHAELVTITVPL
CCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHCHHHHHCCCCCCCCCCCEEEEEEEEC
TAPLTATSKTQLAETLPSSARINRSGVMMEFPQFAQQTMSNKESAQQTSNAEKLAEKTGG
CCCCCCCCHHHHHHHCCCCCEECCCCCEEECHHHHHHHHCCHHHHHHHCCHHHHHHHCCC
ASEFVPLFGVGARSDSAANSGANSNFKYEAESESGLSNTLYCIHNAKDSSQHGVDTLGDA
CCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
SYSAFYLGKGKIAEEPLYIPATEQHEAYLLMTWLDYHNAQSGLSLFRASDISAGPIASAH
CCEEEEECCCCCCCCCEECCCCCCCCEEEEEEEECCCCCHHHHHHEEECCCCCCCCCHHH
MNRVLPLGFHGCFINA
HCCCCEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]