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The map label for this gene is puuA [H]

Identifier: 160874317

GI number: 160874317

Start: 1420496

End: 1421851

Strand: Direct

Name: puuA [H]

Synonym: Sbal195_1198

Alternate gene names: 160874317

Gene position: 1420496-1421851 (Clockwise)

Preceding gene: 160874316

Following gene: 160874318

Centisome position: 26.56

GC content: 47.79

Gene sequence:

>1356_bases
ATGAATAAGCTAATCGCTTTTTTAAAAGAACGAAAAATCACAGAAGTCGAATGTGTTATCAGTGACATGACTGGCATCGC
CCGCGGCAAGATTGCACCAGTCGATAAGTTTCTCGATGAAAAAGGCATGCGTTTGCCCGAAAGCGTGCTGTTACAAACCG
TCACCGGCGATTTTGTGAATGAAGATGTTTACTACAGCTTGCTCGATGCTGCCGACATCGATTTTGTCTGTGTACCCGAT
GAAAACGCTGTCTTTATGTTGCCTTGGACCATAGAAGCCACGGCGCAGGTGATCCACGATTGCTACGACAGAATGGGTAA
TCCGATTGAGCTGTCACCACGTAACGTATTGAAAAAAGTCTTGTCTCTCTATGACGAACAGGGCTGGGAACCGGTTATCG
CTCCTGAAATGGAGTTCTATTTGACCCGTCGTAGTGACGACCACGATTTACCCCTAAAGCCACCAATTGGCCGTTCAGGT
CGACCCGAAGCGGGTCGCCAGTCTTTCTCTATCGATGCCGCCAACGAATACGATCCTATGTTTGAAGACATGTACGATTG
GTGTGAGCTTCAAGGTTTAGATATCGATACTTTAATCCATGAAGATGGCCCCGCGCAGATGGAGATTAACTTCAGCCATG
GCAATCCATTGTCCCTTGCTGATCAAGTGTTTATCTTTAAACGTACCTTGCGCGAAGCGGCACTGAAGCACGATGTTTGC
GCTACCTTTATGGCCAAACCTGTGACTGACGAACCTGGTAGTGCAATGCACATTCACCAGAGTGTGATTAATAAAGAGAC
AGGCAAAAATATTTTCACCAATGAAGATGGCACTAAGAGCCAGTTATTCTTGGGATATATCGCCGGTCTGCAAAAATTCA
TCCCTGAGTTGCTGCCGCTGATGGCGCCCAATGCTAACTCTTTCCGTCGTTTCCTACCCGGCACTTCAGCGCCAGTGAAC
TTAGAGTGGGGCGATGAAAACCGCACCTGTGGATTACGTATTCCTGAGTCTTCACCACAAAACCGTCGTATCGAAAACCG
TATTCCCGGCGCCGATGCTAACTGTTATTTGGCAATTGCCGCGAGCTTATTGTGTGGTTATATCGGCATGGTTGAGCAGC
TAAAACCTTCGACGCCAGTGCAGGGTAAAGCCAACGAAAGCCGCAGCAATAATACCCATTGTTTACCGCTGACTTTAGAA
GAAGCGTTAGCGGCGATGGAAGAAAGTGATGCCTGTAAGCAGTATTTAGGTGAAGCCTTCACCACAGGTTTTGTGGCGGT
GAAACAGGCCGAACTTGAAAACTTCCGCCGTGTGGTCAGCTCATGGGAACGTGAATTCCTACTGCTCACAGTGTAA

Upstream 100 bases:

>100_bases
ATCCAGAGTGGAAGGTGAGCGAAAATCCGTTTTACCTCTCCCTTTTCAATGCCTTCGGTGATGCATGCCGACGCAGGGCT
ACAACTCGAGTGAAATAATA

Downstream 100 bases:

>100_bases
TGTTTTTGGGATTGAGTGTACTGACAGTGTTGCCGCGTTAACCCAAGGAAAACTGAGGTTAACGTCGGTCATCCCAAGAC
TGAGCTTTGCACTGTTTTAG

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVNEDVYYSLLDAADIDFVCVPD
ENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKVLSLYDEQGWEPVIAPEMEFYLTRRSDDHDLPLKPPIGRSG
RPEAGRQSFSIDAANEYDPMFEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFIFKRTLREAALKHDVC
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTKSQLFLGYIAGLQKFIPELLPLMAPNANSFRRFLPGTSAPVN
LEWGDENRTCGLRIPESSPQNRRIENRIPGADANCYLAIAASLLCGYIGMVEQLKPSTPVQGKANESRSNNTHCLPLTLE
EALAAMEESDACKQYLGEAFTTGFVAVKQAELENFRRVVSSWEREFLLLTV

Sequences:

>Translated_451_residues
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVNEDVYYSLLDAADIDFVCVPD
ENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKVLSLYDEQGWEPVIAPEMEFYLTRRSDDHDLPLKPPIGRSG
RPEAGRQSFSIDAANEYDPMFEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFIFKRTLREAALKHDVC
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTKSQLFLGYIAGLQKFIPELLPLMAPNANSFRRFLPGTSAPVN
LEWGDENRTCGLRIPESSPQNRRIENRIPGADANCYLAIAASLLCGYIGMVEQLKPSTPVQGKANESRSNNTHCLPLTLE
EALAAMEESDACKQYLGEAFTTGFVAVKQAELENFRRVVSSWEREFLLLTV
>Mature_451_residues
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVNEDVYYSLLDAADIDFVCVPD
ENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKVLSLYDEQGWEPVIAPEMEFYLTRRSDDHDLPLKPPIGRSG
RPEAGRQSFSIDAANEYDPMFEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFIFKRTLREAALKHDVC
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTKSQLFLGYIAGLQKFIPELLPLMAPNANSFRRFLPGTSAPVN
LEWGDENRTCGLRIPESSPQNRRIENRIPGADANCYLAIAASLLCGYIGMVEQLKPSTPVQGKANESRSNNTHCLPLTLE
EALAAMEESDACKQYLGEAFTTGFVAVKQAELENFRRVVSSWEREFLLLTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=432, Percent_Identity=28.2407407407407, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI87081870, Length=437, Percent_Identity=34.7826086956522, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1790301, Length=447, Percent_Identity=25.9507829977629, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50536; Mature: 50536

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVN
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
EDVYYSLLDAADIDFVCVPDENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKV
CHHHHHHHHCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LSLYDEQGWEPVIAPEMEFYLTRRSDDHDLPLKPPIGRSGRPEAGRQSFSIDAANEYDPM
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHH
FEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFIFKRTLREAALKHDVC
HHHHHHHHHCCCCCHHHEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTKSQLFLGYIAGLQKFIPELLPL
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MAPNANSFRRFLPGTSAPVNLEWGDENRTCGLRIPESSPQNRRIENRIPGADANCYLAIA
CCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHH
ASLLCGYIGMVEQLKPSTPVQGKANESRSNNTHCLPLTLEEALAAMEESDACKQYLGEAF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
TTGFVAVKQAELENFRRVVSSWEREFLLLTV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MNKLIAFLKERKITEVECVISDMTGIARGKIAPVDKFLDEKGMRLPESVLLQTVTGDFVN
CHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
EDVYYSLLDAADIDFVCVPDENAVFMLPWTIEATAQVIHDCYDRMGNPIELSPRNVLKKV
CHHHHHHHHCCCCCEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECHHHHHHHH
LSLYDEQGWEPVIAPEMEFYLTRRSDDHDLPLKPPIGRSGRPEAGRQSFSIDAANEYDPM
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHH
FEDMYDWCELQGLDIDTLIHEDGPAQMEINFSHGNPLSLADQVFIFKRTLREAALKHDVC
HHHHHHHHHCCCCCHHHEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ATFMAKPVTDEPGSAMHIHQSVINKETGKNIFTNEDGTKSQLFLGYIAGLQKFIPELLPL
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MAPNANSFRRFLPGTSAPVNLEWGDENRTCGLRIPESSPQNRRIENRIPGADANCYLAIA
CCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHH
ASLLCGYIGMVEQLKPSTPVQGKANESRSNNTHCLPLTLEEALAAMEESDACKQYLGEAF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHH
TTGFVAVKQAELENFRRVVSSWEREFLLLTV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]