Click here to switch to the map view.

The map label for this gene is puuD [H]

Identifier: 160874316

GI number: 160874316

Start: 1419732

End: 1420493

Strand: Direct

Name: puuD [H]

Synonym: Sbal195_1197

Alternate gene names: 160874316

Gene position: 1419732-1420493 (Clockwise)

Preceding gene: 160874315

Following gene: 160874317

Centisome position: 26.55

GC content: 48.29

Gene sequence:

>762_bases
ATGTCAGCCGATTTGCCCCTCATAGGTGTTATTGCTTGTAATCAACAACTGGGTTCACATCCCTTTAATATTGTTGGTGA
AAAATACCTTTTAGGTGTGGTTAATGGCGCGAAAGGCTGGCCGTTAGTGATCCCATCGTTAGGCGGCGATTTACCGATAG
ACGCGATTTTAGAACGTCTTGACGGTATTTTGTTTACTGGCTCTCCTTCTAATGTAGAACCCCATCTTTATGCAGGCCAA
CCGAGCGAGCTTGGTACACACCATGATCCTAAACGTGATGCGACGACTTTGCCGCTGATCCACGCTGCTATTGCTGCTGG
GGTACCTGTATTAGGTATTTGTCGCGGTTTTCAAGAAATGAATGTGGCCTTTGGTGGCAGTTTGCACCAGAAGTTATATG
AGGTGGGTGGCTTTATTGAACATAGAGAAGATAAAACCGCATCCTTAGATGTGCAATATGGGGCTTCTCACAGTATTACT
GTTGAGCCCGGGGGGCTTATTTACGAGGCCTGGGGCCGTAACTCTGCAGAGGTCAACTCTGTGCATACTCAAGGCGTGGA
GCGTCTGGGTGTTGGGTTGCGGCCAGAAGCTTATGCTCCAGATGGGTTAGTGGAGGCTTTCTCCGTAATAGGTACTAAAG
AATTTGCCCTCGGTGTGCAATGGCATCCAGAGTGGAAGGTGAGCGAAAATCCGTTTTACCTCTCCCTTTTCAATGCCTTC
GGTGATGCATGCCGACGCAGGGCTACAACTCGAGTGAAATAA

Upstream 100 bases:

>100_bases
CCTCTTGCCTAGTATGAAATTTCGGTGTACTGTGTGAAAAAATGAACATTTAAAGGCAAAGTGTTCGTTATGCTAGTAAA
ACGAATTCTAAAGGTGAGAT

Downstream 100 bases:

>100_bases
TAATGAATAAGCTAATCGCTTTTTTAAAAGAACGAAAAATCACAGAAGTCGAATGTGTTATCAGTGACATGACTGGCATC
GCCCGCGGCAAGATTGCACC

Product: peptidase C26

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSADLPLIGVIACNQQLGSHPFNIVGEKYLLGVVNGAKGWPLVIPSLGGDLPIDAILERLDGILFTGSPSNVEPHLYAGQ
PSELGTHHDPKRDATTLPLIHAAIAAGVPVLGICRGFQEMNVAFGGSLHQKLYEVGGFIEHREDKTASLDVQYGASHSIT
VEPGGLIYEAWGRNSAEVNSVHTQGVERLGVGLRPEAYAPDGLVEAFSVIGTKEFALGVQWHPEWKVSENPFYLSLFNAF
GDACRRRATTRVK

Sequences:

>Translated_253_residues
MSADLPLIGVIACNQQLGSHPFNIVGEKYLLGVVNGAKGWPLVIPSLGGDLPIDAILERLDGILFTGSPSNVEPHLYAGQ
PSELGTHHDPKRDATTLPLIHAAIAAGVPVLGICRGFQEMNVAFGGSLHQKLYEVGGFIEHREDKTASLDVQYGASHSIT
VEPGGLIYEAWGRNSAEVNSVHTQGVERLGVGLRPEAYAPDGLVEAFSVIGTKEFALGVQWHPEWKVSENPFYLSLFNAF
GDACRRRATTRVK
>Mature_252_residues
SADLPLIGVIACNQQLGSHPFNIVGEKYLLGVVNGAKGWPLVIPSLGGDLPIDAILERLDGILFTGSPSNVEPHLYAGQP
SELGTHHDPKRDATTLPLIHAAIAAGVPVLGICRGFQEMNVAFGGSLHQKLYEVGGFIEHREDKTASLDVQYGASHSITV
EPGGLIYEAWGRNSAEVNSVHTQGVERLGVGLRPEAYAPDGLVEAFSVIGTKEFALGVQWHPEWKVSENPFYLSLFNAFG
DACRRRATTRVK

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=250, Percent_Identity=43.6, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 27216; Mature: 27085

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADLPLIGVIACNQQLGSHPFNIVGEKYLLGVVNGAKGWPLVIPSLGGDLPIDAILERL
CCCCCCEEEEEEECHHHCCCCCHHCCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHHHH
DGILFTGSPSNVEPHLYAGQPSELGTHHDPKRDATTLPLIHAAIAAGVPVLGICRGFQEM
CCEEEECCCCCCCCEEECCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCHHHH
NVAFGGSLHQKLYEVGGFIEHREDKTASLDVQYGASHSITVEPGGLIYEAWGRNSAEVNS
HHHCCCHHHHHHHHHCCHHCCCCCCCEEEEEEECCCCEEEECCCCEEEECCCCCCCHHHH
VHTQGVERLGVGLRPEAYAPDGLVEAFSVIGTKEFALGVQWHPEWKVSENPFYLSLFNAF
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHEEEEEECCCCEECCCCEEEEHHHHH
GDACRRRATTRVK
HHHHHHHHHCCCC
>Mature Secondary Structure 
SADLPLIGVIACNQQLGSHPFNIVGEKYLLGVVNGAKGWPLVIPSLGGDLPIDAILERL
CCCCCEEEEEEECHHHCCCCCHHCCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHHHH
DGILFTGSPSNVEPHLYAGQPSELGTHHDPKRDATTLPLIHAAIAAGVPVLGICRGFQEM
CCEEEECCCCCCCCEEECCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCHHHH
NVAFGGSLHQKLYEVGGFIEHREDKTASLDVQYGASHSITVEPGGLIYEAWGRNSAEVNS
HHHCCCHHHHHHHHHCCHHCCCCCCCEEEEEEECCCCEEEECCCCEEEECCCCCCCHHHH
VHTQGVERLGVGLRPEAYAPDGLVEAFSVIGTKEFALGVQWHPEWKVSENPFYLSLFNAF
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHEEEEEECCCCEECCCCEEEEHHHHH
GDACRRRATTRVK
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]