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The map label for this gene is potF [H]

Identifier: 160874318

GI number: 160874318

Start: 1422454

End: 1423554

Strand: Direct

Name: potF [H]

Synonym: Sbal195_1199

Alternate gene names: 160874318

Gene position: 1422454-1423554 (Clockwise)

Preceding gene: 160874317

Following gene: 160874319

Centisome position: 26.6

GC content: 44.32

Gene sequence:

>1101_bases
ATGAAGCTATTTAATAAGATGACCACTCTAGCTCTGGTAACTGCGAGCGTATTAGCGAGCGCAGCGGCCCAAGCGGAAGA
AGTGGTTCGCGTGTATAACTGGTCAGATTATATCGCGGAAGATACCTTAGAAAACTTCAAGAAAGAAACGGGCATTCGGG
TTATTTACGATGTGTTCGATAGTAACGAAGTGCTTGAGGCTAAATTATTGTCTGGTCGAAGTGGCTACGATATTGTTGTC
CCTTCTAACCACTTTCTCGCTAAGCAAATCAAAGCGGGTGCTTTCAAACCTTTAGACCGCGCTAAGCTATCTAATTTCAA
AAATTTAAATCCCGCCCTGATGAAGCTACTTGAGAAAGCCGATCCGGGTAACCAGTATGCAGTGCCTTATTTATGGGGAA
CCAATGGTATTGGTTACAACATCGATAAAGTGAAAGCGGCTGTGGGTGAAGATGCGCCATTCAACTCAATGGAACTGATC
TTCAATCCTAAATATGCTGAAAAAATCTCTAAGTGTGGCTTTGCTATGCTGGACTCTGCCGACGATATGGTGCCCCAAGC
ACTGATTTATTTAGGTTTAGATCCTAACAGTTCCAACCCAAGCGATTATGAAAAAGCCGGTGAGTTACTGGAAAAAATCC
GTCCTTACGTGACCTATTTCCACTCATCTCGCTATATTTCTGACTTAGCAAACGGTGACATTTGTGTGGCCTTTGGTTTT
TCTGGTGACGTATTCCAAGCTAAAGCGCGTGCTGAAGAGGCGGGTAATGGCAATAAGATTGGTTACTCGATTCCAAAAGA
AGGCGCTAACCTGTGGTTTGATATGTTAGCTATCCCAGCCGATTCGACTAACGCAGATAATGCACTGACGCTGATTAACT
ATTTCCTCCGTCCAGAAGTCATAGCGCCTATCTCTAACTATGTGGCCTATGCTAACCCGAACGATCCTGCACAACCTCTG
GTTGATGAGGCTATCCGCACCGATCCCGCGATTTATCCACCGCAAGAAGTGTTAGATAAACTTTATATTGGTGAAATCCG
TCCTTTGAAAATCCAACGCGTATTAACCCGTGTTTGGACCAAAGTGAAGTCAGGACAATAG

Upstream 100 bases:

>100_bases
GGTGTAGCATTGGTCGAAAATAACAACAATATCCGGATAGCCCGGTGACACAATGTCAGAATTTGCTTAAGCAATTCTGC
TACCCGAGAAAGGAGAAAGC

Downstream 100 bases:

>100_bases
TCGATAAGGGGCAAGCCTAGCTTGCCCCTAGCTTGGCAGACTTGCTATGTCCAATCCTATGCATAGTTCATCCACGTATA
GGATTTGACACAGTTTGGTT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFDSNEVLEAKLLSGRSGYDIVV
PSNHFLAKQIKAGAFKPLDRAKLSNFKNLNPALMKLLEKADPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELI
FNPKYAEKISKCGFAMLDSADDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEVIAPISNYVAYANPNDPAQPL
VDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWTKVKSGQ

Sequences:

>Translated_366_residues
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFDSNEVLEAKLLSGRSGYDIVV
PSNHFLAKQIKAGAFKPLDRAKLSNFKNLNPALMKLLEKADPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELI
FNPKYAEKISKCGFAMLDSADDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEVIAPISNYVAYANPNDPAQPL
VDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWTKVKSGQ
>Mature_366_residues
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFDSNEVLEAKLLSGRSGYDIVV
PSNHFLAKQIKAGAFKPLDRAKLSNFKNLNPALMKLLEKADPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELI
FNPKYAEKISKCGFAMLDSADDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEVIAPISNYVAYANPNDPAQPL
VDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWTKVKSGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=370, Percent_Identity=53.2432432432432, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1787367, Length=339, Percent_Identity=33.6283185840708, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1787711, Length=355, Percent_Identity=23.0985915492958, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40476; Mature: 40476

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
SNEVLEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLDRAKLSNFKNLNPALMKLLEKA
CCHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHC
DPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELIFNPKYAEKISKCGFAMLDSA
CCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHCCC
DDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
HHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEV
CCCHHHHHHHHHHCCCCCEECCCCCCCCHHHEEHHHEECCCCCCCCHHHHHHHHHHCCHH
IAPISNYVAYANPNDPAQPLVDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWT
HHCHHHCEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
KVKSGQ
HHCCCC
>Mature Secondary Structure
MKLFNKMTTLALVTASVLASAAAQAEEVVRVYNWSDYIAEDTLENFKKETGIRVIYDVFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECC
SNEVLEAKLLSGRSGYDIVVPSNHFLAKQIKAGAFKPLDRAKLSNFKNLNPALMKLLEKA
CCHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHC
DPGNQYAVPYLWGTNGIGYNIDKVKAAVGEDAPFNSMELIFNPKYAEKISKCGFAMLDSA
CCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHCCHHHHCCC
DDMVPQALIYLGLDPNSSNPSDYEKAGELLEKIRPYVTYFHSSRYISDLANGDICVAFGF
HHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
SGDVFQAKARAEEAGNGNKIGYSIPKEGANLWFDMLAIPADSTNADNALTLINYFLRPEV
CCCHHHHHHHHHHCCCCCEECCCCCCCCHHHEEHHHEECCCCCCCCHHHHHHHHHHCCHH
IAPISNYVAYANPNDPAQPLVDEAIRTDPAIYPPQEVLDKLYIGEIRPLKIQRVLTRVWT
HHCHHHCEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
KVKSGQ
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]