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The map label for this gene is napB [H]

Identifier: 160873979

GI number: 160873979

Start: 1012319

End: 1012750

Strand: Reverse

Name: napB [H]

Synonym: Sbal195_0858

Alternate gene names: 160873979

Gene position: 1012750-1012319 (Counterclockwise)

Preceding gene: 160873980

Following gene: 160873977

Centisome position: 18.94

GC content: 48.61

Gene sequence:

>432_bases
ATGAGAAAAATACTCACCTTAACTGCGTTACTCGTCGCCATTACCGGCTGTTCCGGTCAGCAAACCGACACCAATGCAGC
GCCAGTTAATATTCAATCCCTCGCAGGAAACATGGCGATCACCGACATTCGCCCAGCCGATGCCATGGCGATTTATCCCA
ATCGCGGCACCGCCATTGAGCGCAGCTTTAGCGAGCAACCACCGCTCATCCCACATAAGGACGATTACAAGATCACCTTA
GATAAAAATGGCTGTCTAAGCTGCCATAGTTGGGACAAAGCAAAACGCATGAAAGCCACGCCTGTTGCTAAGTCCCATGT
GATTGATGATAAAGGCACAGTTAACGGGCATAACTATTTCTGTACTCAATGCCACGTCGCTCAAGCCGAAAACAAACAAC
CACTCGTGGAGAATCAGTTCTCAGACAAGTAA

Upstream 100 bases:

>100_bases
AGTTAGTCAACAAACTGATATTGGATGCGACCGATCCCCTGTCAAAAGAAACTGACTTTAAAAAATGCCCAGTGAAAGTC
TTCAAGGCGGAGAATAACCA

Downstream 100 bases:

>100_bases
ATCACCTTCCCCTGCAAAGCAAAGCCACCCGAATTTATGAGGGTGGCTTTTTTGCTATTTAAGCGTCCTATCCTAATGAG
TAGGCTTAGAGTCAGTAATG

Product: nitrate reductase cytochrome c-type subunit NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MRKILTLTALLVAITGCSGQQTDTNAAPVNIQSLAGNMAITDIRPADAMAIYPNRGTAIERSFSEQPPLIPHKDDYKITL
DKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYFCTQCHVAQAENKQPLVENQFSDK

Sequences:

>Translated_143_residues
MRKILTLTALLVAITGCSGQQTDTNAAPVNIQSLAGNMAITDIRPADAMAIYPNRGTAIERSFSEQPPLIPHKDDYKITL
DKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYFCTQCHVAQAENKQPLVENQFSDK
>Mature_143_residues
MRKILTLTALLVAITGCSGQQTDTNAAPVNIQSLAGNMAITDIRPADAMAIYPNRGTAIERSFSEQPPLIPHKDDYKITL
DKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYFCTQCHVAQAENKQPLVENQFSDK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=85, Percent_Identity=36.4705882352941, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 15667; Mature: 15667

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILTLTALLVAITGCSGQQTDTNAAPVNIQSLAGNMAITDIRPADAMAIYPNRGTAIE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHCCCEEEEECCCCCEEEEECCCCCEEE
RSFSEQPPLIPHKDDYKITLDKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYF
CCCCCCCCCCCCCCCCEEEECCCCCEECCCCCCHHCEECCCCCCCEEECCCCCCCCCCEE
CTQCHVAQAENKQPLVENQFSDK
EEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKILTLTALLVAITGCSGQQTDTNAAPVNIQSLAGNMAITDIRPADAMAIYPNRGTAIE
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHCCCEEEEECCCCCEEEEECCCCCEEE
RSFSEQPPLIPHKDDYKITLDKNGCLSCHSWDKAKRMKATPVAKSHVIDDKGTVNGHNYF
CCCCCCCCCCCCCCCCEEEECCCCCEECCCCCCHHCEECCCCCCCEEECCCCCCCCCCEE
CTQCHVAQAENKQPLVENQFSDK
EEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]