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The map label for this gene is napA [H]

Identifier: 160873980

GI number: 160873980

Start: 1012747

End: 1015242

Strand: Reverse

Name: napA [H]

Synonym: Sbal195_0859

Alternate gene names: 160873980

Gene position: 1015242-1012747 (Counterclockwise)

Preceding gene: 160873981

Following gene: 160873979

Centisome position: 18.99

GC content: 50.08

Gene sequence:

>2496_bases
ATGAGCATAAGCCGCCGCGAGTTTCTCAAGGCCAACGCAGCGGTTGCCGCCGCAACAGCCGTTGGTGTTACGCTTCCCAT
TAAAATGGTTGAAGCCGCCGAACAGCCAGACAATATCAAATGGGATAAAGCCCCTTGCCGCTTCTGCGGTGTGGGTTGTA
GCGTATTAGTCGGCACCAAAGCGGGTAAAGTCGTTGCTACTAAGGGCGATCCTGAAAGTCCAGTCAACCGTGGCCTTAAC
TGTATTAAAGGTTATTTCCTCTCAAAAATCATGTACGGCAAGGACAGATTAACCACGCCCTTGCTGCGGATGAAAGACGG
CAAATACCATAAAGAAGGCGAGTTCACCCCAATCAGTTGGGATATGGCCTTTGATACTATGGCGGCTAAATGGAAACACA
GTCTCGCCACCAAAGGCCCGACGTCTGTAGGTATGTTTGGTTCAGGTCAATGGACTATTTGGGAAGGTTATGCCGCCTCT
AAACTTCATAAAGCAGGTTTCCTTACCAACAATATCGACCCTAACGCACGCCACTGCATGGCCTCTGCCGTAGGCGGCTT
TATGCGCACCTTCGGTATCGATGAACCCATGGGTTGTTATGACGATTTAGAAGCCGCCGATCAGTTTGTGCTTTGGGGCG
CGAACATGGCCGAGATGCACCCAATTCTATGGGCACGTTTATCCGATAGACGTTTAAGCAGTCCAACTTGCCGCGTGCAT
GTGCTGTCGACCTTTGAAAATCGCAGCTTCGATTTAGCCGATAATCCTATGGTGTTCCGCCCGCAATCGGACTTAGTGAT
CCTCAACTTTATTGCCAATTACATTATTCAAAATAACGCGGTTAATACCGACTTTGTCACTAAACACACTAAGTTTGCCT
TAGGCGTTGACGATATCGGCTATGGCCTGCGTCCAGATCATCCATTAGAGAAAAAAGCCAAAAATCCAGGCAATGGTAAA
TCGACACCGATTAGCTTTGACGAGTATGCCAAGTTTGTCAGCACATATACGCTGGAATACGCGGCTAAGATGAGTGGTGT
GGAGCCCGAGAAATTAGCAACCTTAGCCAAGGCCTATGCCGATCCTAACGTTAAAGTGATGAGTCTGTGGACCATGGGCA
TTAACCAACATGTTCGCGGCGTGTGGGCAAACAACATGCTCTACAACATCCACTTACTCACGGGCAAAATTGCTACACCG
GGTAACAGTCCATTCTCGTTAACGGGTCAACCTTCGGCTTGCGGGACTGCCCGTGAAGTGGGGACGTTCGCCCATCGTTT
ACCCGCCGATATGGAAGTGACCAACGACAAGCACCGCGCCATTACCGAAAAGCTATGGCAAGTGCCTGCTGGGACGATTC
CCGCAAAACCTGGCTACCATGCCGTACTCCAGAGCCGTATGCTCAAAGATGGCAAGCTCAACTGCTACTGGACCATGTGT
ACCAATAATATGCAAGCTGGCCCTAACATCAACGATGAAATCTATCCGGGTTTCCGTAATCCAGAAAACTTTATCGTGGT
TTCAGATCCCTACCCAACCATCACAGCCATGGCGGCGGATTTGATTTTGCCAACAGCAATGTGGGTCGAAAAAGAAGGAG
CTTACGGCAATGCCGAGCGCCGTACTCATATGTGGCATCAGCAAGTAAAAGCCCCCGAAGGCGCAACCTCAGATCTGTGG
CAACTAGTCGAGTTTTCAAAACGTTTCAAAGTCTCCGAAGTCTGGCCAGCCGAACTGATAGCCAAACAACCGGAATACGC
GGATAAAACCTTATATGAAGTGCTATTTGCCAATGGCGTGATCGACAAGTTCCCCACATCCGATTGCAAAGCCGAATTAA
ACGATGAGAGCGAACACTTTGGTTTCTATCTGCAAAAAGGCATTTTTGAAGAATACGCCACATTCGGTCGTGGTCACGGC
CATGATCTAGCTGACTTTGACCGTTACCACGAAACCCGCGGCCTACGCTGGCCAGTCGTTAATGGCAAAGAAACCCTGCG
CCGTTTCGTTGAGGGGAGCGACCCTTATGTCAAAGCGGGTGAAGGCTTTAAATTCTATGGCAAGCCAGATGGTAAAGCGG
TGATCTTCGCCCTGCCCTACGAACCCGCCGCCGAAGAGCCGAATAAAGAATACGATCTGTGGATGTCGACTGGCCGCGTG
CTGGAACATTGGCACACAGGCTCGATGACAGCCCGCGTACCTGAGCTTTACCGCGCCTTTCCCGACGCGCAGATTTTTAT
GCACCCAGAAGATGCCAAAGCCCGCGGTGTACAACGCGGTGATGAAGTCATAGTCGCATCACCACGGGGTGAAGTAAAAA
CTCGTGTCGAGACCAAAGGCCGTAACAAACCGCCACGCGGCGTGGTATTTATGCCTTTCTTCGATGCACGCCAGTTAGTC
AACAAACTGATATTGGATGCGACCGATCCCCTGTCAAAAGAAACTGACTTTAAAAAATGCCCAGTGAAAGTCTTCAAGGC
GGAGAATAACCAATGA

Upstream 100 bases:

>100_bases
TCGACAATATTGAAGCCATCAATGCCCTGTCCGGCGTGTTATCTGCCAGTTTGATTTACCACCAAGTCGCACCTTTAGAA
CAAGAGAGTGAGGAAAGATT

Downstream 100 bases:

>100_bases
GAAAAATACTCACCTTAACTGCGTTACTCGTCGCCATTACCGGCTGTTCCGGTCAGCAAACCGACACCAATGCAGCGCCA
GTTAATATTCAATCCCTCGC

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 831; Mature: 830

Protein sequence:

>831_residues
MSISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTKAGKVVATKGDPESPVNRGLN
CIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISWDMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAAS
KLHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVH
VLSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIGYGLRPDHPLEKKAKNPGNGK
STPISFDEYAKFVSTYTLEYAAKMSGVEPEKLATLAKAYADPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMEVTNDKHRAITEKLWQVPAGTIPAKPGYHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGATSDLW
QLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVIDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRGHG
HDLADFDRYHETRGLRWPVVNGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNKEYDLWMSTGRV
LEHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLV
NKLILDATDPLSKETDFKKCPVKVFKAENNQ

Sequences:

>Translated_831_residues
MSISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTKAGKVVATKGDPESPVNRGLN
CIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISWDMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAAS
KLHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVH
VLSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIGYGLRPDHPLEKKAKNPGNGK
STPISFDEYAKFVSTYTLEYAAKMSGVEPEKLATLAKAYADPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMEVTNDKHRAITEKLWQVPAGTIPAKPGYHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGATSDLW
QLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVIDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRGHG
HDLADFDRYHETRGLRWPVVNGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNKEYDLWMSTGRV
LEHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLV
NKLILDATDPLSKETDFKKCPVKVFKAENNQ
>Mature_830_residues
SISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTKAGKVVATKGDPESPVNRGLNC
IKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISWDMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAASK
LHKAGFLTNNIDPNARHCMASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVHV
LSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIGYGLRPDHPLEKKAKNPGNGKS
TPISFDEYAKFVSTYTLEYAAKMSGVEPEKLATLAKAYADPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPG
NSPFSLTGQPSACGTAREVGTFAHRLPADMEVTNDKHRAITEKLWQVPAGTIPAKPGYHAVLQSRMLKDGKLNCYWTMCT
NNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGATSDLWQ
LVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGVIDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRGHGH
DLADFDRYHETRGLRWPVVNGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNKEYDLWMSTGRVL
EHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKGRNKPPRGVVFMPFFDARQLVN
KLILDATDPLSKETDFKKCPVKVFKAENNQ

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=827, Percent_Identity=72.9141475211608, Blast_Score=1275, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=793, Percent_Identity=23.7074401008827, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI3868720, Length=663, Percent_Identity=23.3785822021116, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI3868719, Length=495, Percent_Identity=24.6464646464646, Blast_Score=106, Evalue=7e-24,
Organism=Escherichia coli, GI87081797, Length=832, Percent_Identity=21.6346153846154, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787870, Length=560, Percent_Identity=23.3928571428571, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92861; Mature: 92730

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTK
CCCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHCCCCCCCCCCCCCCHHHCCCCEEEEECC
AGKVVATKGDPESPVNRGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCEEEH
DMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAASKLHKAGFLTNNIDPNARHCM
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVH
HHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEE
VLSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIG
EEEECCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHCCCCCCCCCEECCCEEEECHHHCC
YGLRPDHPLEKKAKNPGNGKSTPISFDEYAKFVSTYTLEYAAKMSGVEPEKLATLAKAYA
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCHHHCEEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMEVTNDKHRAITEKLWQVPAGTIPAKPGYHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGATSDLWQLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
IDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRGHGHDLADFDRYHETRGLRWPVV
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
NGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNKEYDLWMSTGRV
CCHHHHHHHHCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCCEEHHHHHCHH
LEHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKG
HHHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEHHHCC
RNKPPRGVVFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVFKAENNQ
CCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SISRREFLKANAAVAAATAVGVTLPIKMVEAAEQPDNIKWDKAPCRFCGVGCSVLVGTK
CCCHHHHHHHHHHHHHHHHHCEEEHHHHHHHCCCCCCCCCCCCCCHHHCCCCEEEEECC
AGKVVATKGDPESPVNRGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGKYHKEGEFTPISW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCEEEH
DMAFDTMAAKWKHSLATKGPTSVGMFGSGQWTIWEGYAASKLHKAGFLTNNIDPNARHCM
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVGGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTCRVH
HHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEE
VLSTFENRSFDLADNPMVFRPQSDLVILNFIANYIIQNNAVNTDFVTKHTKFALGVDDIG
EEEECCCCCCCCCCCCEEECCCCCEEHHHHHHHHHHCCCCCCCCCEECCCEEEECHHHCC
YGLRPDHPLEKKAKNPGNGKSTPISFDEYAKFVSTYTLEYAAKMSGVEPEKLATLAKAYA
CCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNVKVMSLWTMGINQHVRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCHHHCEEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMEVTNDKHRAITEKLWQVPAGTIPAKPGYHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEIYPGFRNPENFIVVSDPYPTITAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGATSDLWQLVEFSKRFKVSEVWPAELIAKQPEYADKTLYEVLFANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
IDKFPTSDCKAELNDESEHFGFYLQKGIFEEYATFGRGHGHDLADFDRYHETRGLRWPVV
CCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
NGKETLRRFVEGSDPYVKAGEGFKFYGKPDGKAVIFALPYEPAAEEPNKEYDLWMSTGRV
CCHHHHHHHHCCCCCEEECCCCEEEECCCCCCEEEEEECCCCCCCCCCCCEEHHHHHCHH
LEHWHTGSMTARVPELYRAFPDAQIFMHPEDAKARGVQRGDEVIVASPRGEVKTRVETKG
HHHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEHHHCC
RNKPPRGVVFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVFKAENNQ
CCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]