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The map label for this gene is ywbI [H]

Identifier: 160873977

GI number: 160873977

Start: 1010918

End: 1011805

Strand: Reverse

Name: ywbI [H]

Synonym: Sbal195_0856

Alternate gene names: 160873977

Gene position: 1011805-1010918 (Counterclockwise)

Preceding gene: 160873979

Following gene: 160873974

Centisome position: 18.92

GC content: 49.66

Gene sequence:

>888_bases
ATGAACATCCCAATCAAGACCTTACAGTGTTTTCTGACCTTAGTGGAAACTGAAAACTTTACCCGCGCCGCGCAGAAATG
TTTTATCACTCAGCCGACACTGAGCAAAATCATCCAAAGACTCGAGGAGAGCCTAGGGGAAACCTTGCTGATCCGAAATA
ACCAAAAAGTTGAGCTCACTCAGGCGGGGCAGCTATTTGAGCACAGTGCACGAGAAATCCTCGGCCAATGGCATAGATTG
CAGGAGGACATGAGTAACCTCAGCGGTCTCAAATCAGGGCGGTTACGACTGGGTGTATGCCCTATGATGAGCAGCCTTAT
CATAGGCTTGCTCACTAGCTACCGTAAACGTTATCCGGGGGTTGAACTACACATGTATGAGTACGGCGGATTCGGCTGCG
AGCAAGCCCTGCTCAATAACAGTCTCGATATCGCCTTTACCGCGCTACCCACAACCCATGAGACAGAACTCGTCAATCAA
CCCCTGACTAAGTACCCTTTGCTTGCATGCCTGCCTGAGGGGCATCCCTTAGCAGAGAAAGACCACTTAAACTGGCAGGA
CTTCCAACCCTATCCTTTTATCCTCTATAACGAAGATTTCTCTCTCGCTAAGTTGATCAATCGGCTCAGCCGCAAAGCAG
GTGTTCAGCTCAACATTGCCTTTCGCAGCGGCCAGTGGGATTTTCTCGCCGCCATGGTCGAAGCCGATATGGGTGTCGCC
ATTTTACCTGAGCCGATTTGCAATAGACTAAGCACCAGCAAACTGGTCTTTAAACCTATGCAACCCGCAATGACATGGGA
TCTGGCGCTTATTTGGCGACAAAGTTTACCTTTAACGCCCGCGGCCAAGGCCTTGCTCGAATTAAGTAAACAAAAGACTG
AACGCTGA

Upstream 100 bases:

>100_bases
ATTTCATCGGTATTCCCCTGTTTTGTTAACAAGGTCAAAGTAATACTTAGGCCATCATTAGTAAAATGAATAAATTCCAT
AATATGGATTCCATTTGAGC

Downstream 100 bases:

>100_bases
GAAGATAAAGCAAGAACTGACATTCTATTAAACAAAGGGGTACGGAGCTTCGTCAGCCGAAGCTGATAATGACCGTTGGA
TCTCACCCATAAAAAAACCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNIPIKTLQCFLTLVETENFTRAAQKCFITQPTLSKIIQRLEESLGETLLIRNNQKVELTQAGQLFEHSAREILGQWHRL
QEDMSNLSGLKSGRLRLGVCPMMSSLIIGLLTSYRKRYPGVELHMYEYGGFGCEQALLNNSLDIAFTALPTTHETELVNQ
PLTKYPLLACLPEGHPLAEKDHLNWQDFQPYPFILYNEDFSLAKLINRLSRKAGVQLNIAFRSGQWDFLAAMVEADMGVA
ILPEPICNRLSTSKLVFKPMQPAMTWDLALIWRQSLPLTPAAKALLELSKQKTER

Sequences:

>Translated_295_residues
MNIPIKTLQCFLTLVETENFTRAAQKCFITQPTLSKIIQRLEESLGETLLIRNNQKVELTQAGQLFEHSAREILGQWHRL
QEDMSNLSGLKSGRLRLGVCPMMSSLIIGLLTSYRKRYPGVELHMYEYGGFGCEQALLNNSLDIAFTALPTTHETELVNQ
PLTKYPLLACLPEGHPLAEKDHLNWQDFQPYPFILYNEDFSLAKLINRLSRKAGVQLNIAFRSGQWDFLAAMVEADMGVA
ILPEPICNRLSTSKLVFKPMQPAMTWDLALIWRQSLPLTPAAKALLELSKQKTER
>Mature_295_residues
MNIPIKTLQCFLTLVETENFTRAAQKCFITQPTLSKIIQRLEESLGETLLIRNNQKVELTQAGQLFEHSAREILGQWHRL
QEDMSNLSGLKSGRLRLGVCPMMSSLIIGLLTSYRKRYPGVELHMYEYGGFGCEQALLNNSLDIAFTALPTTHETELVNQ
PLTKYPLLACLPEGHPLAEKDHLNWQDFQPYPFILYNEDFSLAKLINRLSRKAGVQLNIAFRSGQWDFLAAMVEADMGVA
ILPEPICNRLSTSKLVFKPMQPAMTWDLALIWRQSLPLTPAAKALLELSKQKTER

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=289, Percent_Identity=26.9896193771626, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787879, Length=292, Percent_Identity=28.7671232876712, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790399, Length=293, Percent_Identity=27.3037542662116, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=26.1904761904762, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1788748, Length=293, Percent_Identity=24.9146757679181, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=25, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788297, Length=256, Percent_Identity=28.515625, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI2367136, Length=284, Percent_Identity=22.887323943662, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790208, Length=263, Percent_Identity=25.8555133079848, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789204, Length=272, Percent_Identity=26.1029411764706, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787530, Length=240, Percent_Identity=22.0833333333333, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790783, Length=291, Percent_Identity=26.1168384879725, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=173, Percent_Identity=30.0578034682081, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790262, Length=257, Percent_Identity=25.2918287937743, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=180, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=172, Percent_Identity=23.8372093023256, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789506, Length=287, Percent_Identity=20.5574912891986, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1786713, Length=278, Percent_Identity=23.3812949640288, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082184, Length=148, Percent_Identity=28.3783783783784, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33488; Mature: 33488

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS50931 HTH_LYSR ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPIKTLQCFLTLVETENFTRAAQKCFITQPTLSKIIQRLEESLGETLLIRNNQKVELT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCEEEHH
QAGQLFEHSAREILGQWHRLQEDMSNLSGLKSGRLRLGVCPMMSSLIIGLLTSYRKRYPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
VELHMYEYGGFGCEQALLNNSLDIAFTALPTTHETELVNQPLTKYPLLACLPEGHPLAEK
CEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCHHHHHCCCHHHCCCEEECCCCCCCCCC
DHLNWQDFQPYPFILYNEDFSLAKLINRLSRKAGVQLNIAFRSGQWDFLAAMVEADMGVA
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCEE
ILPEPICNRLSTSKLVFKPMQPAMTWDLALIWRQSLPLTPAAKALLELSKQKTER
ECCHHHHHHHCCCHHEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIPIKTLQCFLTLVETENFTRAAQKCFITQPTLSKIIQRLEESLGETLLIRNNQKVELT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCEEEHH
QAGQLFEHSAREILGQWHRLQEDMSNLSGLKSGRLRLGVCPMMSSLIIGLLTSYRKRYPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
VELHMYEYGGFGCEQALLNNSLDIAFTALPTTHETELVNQPLTKYPLLACLPEGHPLAEK
CEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCHHHHHCCCHHHCCCEEECCCCCCCCCC
DHLNWQDFQPYPFILYNEDFSLAKLINRLSRKAGVQLNIAFRSGQWDFLAAMVEADMGVA
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCEE
ILPEPICNRLSTSKLVFKPMQPAMTWDLALIWRQSLPLTPAAKALLELSKQKTER
ECCHHHHHHHCCCHHEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]