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The map label for this gene is aspA [H]

Identifier: 160873767

GI number: 160873767

Start: 774233

End: 775672

Strand: Reverse

Name: aspA [H]

Synonym: Sbal195_0645

Alternate gene names: 160873767

Gene position: 775672-774233 (Counterclockwise)

Preceding gene: 160873771

Following gene: 160873766

Centisome position: 14.51

GC content: 46.32

Gene sequence:

>1440_bases
ATGAAAGATACTAATAGCGTACGGATCGAAGAAGATTTATTGGGAACGAAAGAAGTGTCCAATGAATTTTATTACGGCAT
ACATACATTACGTGCCATCGAAAACTTTAAGCTGAGTAATCAAAAAATTTCCGATGTACCTGAATTAGTCCGTGGCATGA
TGCTAACAAAGAAAGCTGCTGCCATGGCCAATAGCGAATTAGGCGCCGTAAGTCCTGAAATCGCTGACAAGATTATTGAA
GCGTGCGATTTGATACTCACAACAGGCCGATTTGTGGATCAATTCCCAGTCGACGTGTATCAAGGAGGCGCAGGCACTTC
AGTCAACATGAATACCAATGAGGTATTAGCCAACGTTGCACTGGAGATCATGGGGCTTGAAAAAGGCCGTTACGATGTCA
TCAATCCTAATGACCATGTCAACAAGTGCCAATCGACCAACGATGCCTATCCAACAGGCTTCCGTATTGCGGTCATCAAC
AGTACCGATATTCTGTTAGATGCATTAACTGACCTTATCAGTGCCTTCGACACTAAAGCCGCAGAGTTTAAAACCGTCTT
AAAAATGGGCCGCACTCAGTTGCAAGATGCCGTGCCTATGACCTTAGGCCAAGAGTTTCATGCCTTTGCTGTCACACTGC
GAGAAGAAATTAAGTCCATCAAGCGTTGCCAAGAGTTGTTGTTAGAAGTGAACTTAGGCGCAACGGCCATTGGTACAGGC
CTTAACACGCCAGTGGGTTATTCCTCACTCGCCATTAAACATCTGGCTGAAATCACTGGCCGCGCTTATGTGCCAGCGGA
AGATTTAATCGAAGCTACCTCCGATTGTGGCGCCTACGTCATGCTGCACAGTGCGATTAAGCGCATGGCGATTAAAATGT
CCAAGATTTGTAATGACCTACGCTTACTGTCCTCGGGTCCACGTACAGGTCTGAAGGAGATCAATCTGCCTGAATTACAA
GCAGGTTCTTCTATCATGCCAGCTAAGGTTAACCCTGTGATCCCTGAGGTCGTCAATCAGGTTGCCTTTAAGATTGTCGG
CAACGATATCACTATCACTATGGCCGCCGAAGCGGGCCAGTTACAGCTTAACGTCATGGAACCTGTGATTGGTCAGGCGA
TGTTTGAGTCACTCAGTTTAATGGGTAATGCCTGTATCTCCCTACGTGAGAAATGCGTTGAAGGTATCACTGCAAACCCA
GAGATCTGTATGGCCCATGTGATGAACTCCATCGGGATCGTGACCTACCTTAACCCCATCATCGGCCACCATGAAGGTGA
CATCATCGGCAAGATTTGCGCCCAAACGGGTAAAAATGTCCGTGAAGTGGTACTCGAACGCGGTTTGTTAACGGTTGAAG
AACTCGATGACATTCTCTCGATTGAAAACCTGATGAATCCTAAGTACCAAGCTAAACGCAGTAGCAAAGCCAAAGTGTAA

Upstream 100 bases:

>100_bases
TCGATGAGTTATTTTTGAAGTGTTACGCATACTTATGTACGCACCACAACTCAATACTCGGAGATATATCTTACTATTAA
CCGGATTTAATAGGATCGAA

Downstream 100 bases:

>100_bases
CTCAGTGGGGCTGGGGAACCTTTCCCAGCCCAAAACTCGATGATTCAAGAATAAGCCTGCGCATAGTGAATATGCTCAGG
CTATTGAAATTAAAAACAAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKDTNSVRIEEDLLGTKEVSNEFYYGIHTLRAIENFKLSNQKISDVPELVRGMMLTKKAAAMANSELGAVSPEIADKIIE
ACDLILTTGRFVDQFPVDVYQGGAGTSVNMNTNEVLANVALEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRIAVIN
STDILLDALTDLISAFDTKAAEFKTVLKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTG
LNTPVGYSSLAIKHLAEITGRAYVPAEDLIEATSDCGAYVMLHSAIKRMAIKMSKICNDLRLLSSGPRTGLKEINLPELQ
AGSSIMPAKVNPVIPEVVNQVAFKIVGNDITITMAAEAGQLQLNVMEPVIGQAMFESLSLMGNACISLREKCVEGITANP
EICMAHVMNSIGIVTYLNPIIGHHEGDIIGKICAQTGKNVREVVLERGLLTVEELDDILSIENLMNPKYQAKRSSKAKV

Sequences:

>Translated_479_residues
MKDTNSVRIEEDLLGTKEVSNEFYYGIHTLRAIENFKLSNQKISDVPELVRGMMLTKKAAAMANSELGAVSPEIADKIIE
ACDLILTTGRFVDQFPVDVYQGGAGTSVNMNTNEVLANVALEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRIAVIN
STDILLDALTDLISAFDTKAAEFKTVLKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTG
LNTPVGYSSLAIKHLAEITGRAYVPAEDLIEATSDCGAYVMLHSAIKRMAIKMSKICNDLRLLSSGPRTGLKEINLPELQ
AGSSIMPAKVNPVIPEVVNQVAFKIVGNDITITMAAEAGQLQLNVMEPVIGQAMFESLSLMGNACISLREKCVEGITANP
EICMAHVMNSIGIVTYLNPIIGHHEGDIIGKICAQTGKNVREVVLERGLLTVEELDDILSIENLMNPKYQAKRSSKAKV
>Mature_479_residues
MKDTNSVRIEEDLLGTKEVSNEFYYGIHTLRAIENFKLSNQKISDVPELVRGMMLTKKAAAMANSELGAVSPEIADKIIE
ACDLILTTGRFVDQFPVDVYQGGAGTSVNMNTNEVLANVALEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRIAVIN
STDILLDALTDLISAFDTKAAEFKTVLKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTG
LNTPVGYSSLAIKHLAEITGRAYVPAEDLIEATSDCGAYVMLHSAIKRMAIKMSKICNDLRLLSSGPRTGLKEINLPELQ
AGSSIMPAKVNPVIPEVVNQVAFKIVGNDITITMAAEAGQLQLNVMEPVIGQAMFESLSLMGNACISLREKCVEGITANP
EICMAHVMNSIGIVTYLNPIIGHHEGDIIGKICAQTGKNVREVVLERGLLTVEELDDILSIENLMNPKYQAKRSSKAKV

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=469, Percent_Identity=39.6588486140725, Blast_Score=347, Evalue=1e-95,
Organism=Escherichia coli, GI87082375, Length=472, Percent_Identity=73.5169491525424, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=462, Percent_Identity=37.6623376623377, Blast_Score=310, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17553882, Length=465, Percent_Identity=38.9247311827957, Blast_Score=333, Evalue=8e-92,
Organism=Caenorhabditis elegans, GI32565146, Length=329, Percent_Identity=38.9057750759878, Blast_Score=239, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324993, Length=465, Percent_Identity=38.0645161290323, Blast_Score=318, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24662684, Length=472, Percent_Identity=37.0762711864407, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24640177, Length=467, Percent_Identity=37.2591006423983, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24640179, Length=467, Percent_Identity=37.2591006423983, Blast_Score=321, Evalue=7e-88,
Organism=Drosophila melanogaster, GI78710009, Length=470, Percent_Identity=37.4468085106383, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=32.1120689655172, Blast_Score=271, Evalue=9e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52055; Mature: 52055

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTNSVRIEEDLLGTKEVSNEFYYGIHTLRAIENFKLSNQKISDVPELVRGMMLTKKAA
CCCCCCEEEHHHHCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AMANSELGAVSPEIADKIIEACDLILTTGRFVDQFPVDVYQGGAGTSVNMNTNEVLANVA
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCEECCCHHHHHHHHH
LEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRIAVINSTDILLDALTDLISAFDTKA
HHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCHH
AEFKTVLKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTG
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
LNTPVGYSSLAIKHLAEITGRAYVPAEDLIEATSDCGAYVMLHSAIKRMAIKMSKICNDL
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRTGLKEINLPELQAGSSIMPAKVNPVIPEVVNQVAFKIVGNDITITMAAEAGQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
LQLNVMEPVIGQAMFESLSLMGNACISLREKCVEGITANPEICMAHVMNSIGIVTYLNPI
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGHHEGDIIGKICAQTGKNVREVVLERGLLTVEELDDILSIENLMNPKYQAKRSSKAKV
HCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKDTNSVRIEEDLLGTKEVSNEFYYGIHTLRAIENFKLSNQKISDVPELVRGMMLTKKAA
CCCCCCEEEHHHHCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AMANSELGAVSPEIADKIIEACDLILTTGRFVDQFPVDVYQGGAGTSVNMNTNEVLANVA
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCEECCCHHHHHHHHH
LEIMGLEKGRYDVINPNDHVNKCQSTNDAYPTGFRIAVINSTDILLDALTDLISAFDTKA
HHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCHH
AEFKTVLKMGRTQLQDAVPMTLGQEFHAFAVTLREEIKSIKRCQELLLEVNLGATAIGTG
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
LNTPVGYSSLAIKHLAEITGRAYVPAEDLIEATSDCGAYVMLHSAIKRMAIKMSKICNDL
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRTGLKEINLPELQAGSSIMPAKVNPVIPEVVNQVAFKIVGNDITITMAAEAGQ
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCC
LQLNVMEPVIGQAMFESLSLMGNACISLREKCVEGITANPEICMAHVMNSIGIVTYLNPI
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IGHHEGDIIGKICAQTGKNVREVVLERGLLTVEELDDILSIENLMNPKYQAKRSSKAKV
HCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]