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The map label for this gene is metF [H]

Identifier: 160873684

GI number: 160873684

Start: 651401

End: 652288

Strand: Direct

Name: metF [H]

Synonym: Sbal195_0562

Alternate gene names: 160873684

Gene position: 651401-652288 (Clockwise)

Preceding gene: 160873683

Following gene: 160873686

Centisome position: 12.18

GC content: 50.45

Gene sequence:

>888_bases
ATGGCTTTTCATCACGCACAACATTCACAATCGCTGAACCAGAGCTTGTCTGAGCTTAATGGCGATATTAATGTTTCTTT
TGAGTTTTTTCCGCCATCGACCCCCGAAATGGAACAGATCCTGTGGAACTCTATTCGTCGTTTAGAGCCGCTTAATCCTA
AATTTGTGTCTGTGACCTATGGCGCTAACTCAGGTGTGCGCGACCGCACCCACGGCGTGATTGAGCGTATTCAACAGGAA
ACGGATCTTGTTGCCGCGCCGCACTTAACCTTAGTGGATGCTAGTGATGAAGAGCTATTAGAACTGGCTAAGCATTACTG
GAAATCGGGCATTCGTGACATAGTGGCACTGCGGGGTGATTTACCTGCGGGCAGCCCCAAGCCCACTCGTTTTGCGGTGG
ATTTAGTACGTTTGCTGCGCTCAGTGGCCGACTTTGATATTTCCGTTGCAGCTTATCCAGAAGTGCATCCCGATGCGAGC
AACGCACAGGCGGATTTGATCAACCTTAAGCGTAAGATTGATGCGGGCGCTAGCCGTGCCATTACCCAGTTTTTCTTCGA
TGTGGAATCTTACCTGCGTTTTCGTGACCGCTGCGTTGCCGCCGGTATCGACGTTGAAATCGTGCCAGGCATTTTGCCTG
TGACTAACTTTACCCAGTTAAAACGTTTCGCGGGCATGACCAATGTGTCGTTACCGGGCTGGCTACACAAGCAATTCGAT
GGTTTAGAAAACGATGCCGGTACACGCCAATTGGTGGGCGCAAACGTGGCAATCGACATGGTAAAAGTCCTGTCCCGTGA
AGGCGTGAAGGATTTCCACTTCTACACCTTAAACCGCGCCGAACTGACCTACGCCATTTGCCATACGTTAGGTGTGCGCC
CTAAGTAA

Upstream 100 bases:

>100_bases
CTCAGTTTCTCTAGTATATGGACATATAGACGTCCAAACGTCCATTTGAACTAATTCATACTAGCAAAGAGTAGCGCCAA
GGGCGGTTGAGGAAATACAC

Downstream 100 bases:

>100_bases
AACATCAACAACATAAACCAATAAAAACGCCGCAGATAATGCGGCGTTTTGCTTTGTGTCAGTGGTGTAGGTATTAGATC
CTAAACTGTGCCAGTAGCAC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAFHHAQHSQSLNQSLSELNGDINVSFEFFPPSTPEMEQILWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQQE
TDLVAAPHLTLVDASDEELLELAKHYWKSGIRDIVALRGDLPAGSPKPTRFAVDLVRLLRSVADFDISVAAYPEVHPDAS
NAQADLINLKRKIDAGASRAITQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAGMTNVSLPGWLHKQFD
GLENDAGTRQLVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPK

Sequences:

>Translated_295_residues
MAFHHAQHSQSLNQSLSELNGDINVSFEFFPPSTPEMEQILWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQQE
TDLVAAPHLTLVDASDEELLELAKHYWKSGIRDIVALRGDLPAGSPKPTRFAVDLVRLLRSVADFDISVAAYPEVHPDAS
NAQADLINLKRKIDAGASRAITQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAGMTNVSLPGWLHKQFD
GLENDAGTRQLVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPK
>Mature_294_residues
AFHHAQHSQSLNQSLSELNGDINVSFEFFPPSTPEMEQILWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQQET
DLVAAPHLTLVDASDEELLELAKHYWKSGIRDIVALRGDLPAGSPKPTRFAVDLVRLLRSVADFDISVAAYPEVHPDASN
AQADLINLKRKIDAGASRAITQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAGMTNVSLPGWLHKQFDG
LENDAGTRQLVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPK

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=286, Percent_Identity=32.8671328671329, Blast_Score=135, Evalue=4e-32,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=73.1292517006803, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=30.5755395683453, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25147436, Length=283, Percent_Identity=30.0353356890459, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=31.6326530612245, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321313, Length=290, Percent_Identity=29.6551724137931, Blast_Score=129, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24662658, Length=263, Percent_Identity=26.9961977186312, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32907; Mature: 32776

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFHHAQHSQSLNQSLSELNGDINVSFEFFPPSTPEMEQILWNSIRRLEPLNPKFVSVTY
CCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
GANSGVRDRTHGVIERIQQETDLVAAPHLTLVDASDEELLELAKHYWKSGIRDIVALRGD
CCCCCCCHHHHHHHHHHHHHHCEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
LPAGSPKPTRFAVDLVRLLRSVADFDISVAAYPEVHPDASNAQADLINLKRKIDAGASRA
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHH
ITQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAGMTNVSLPGWLHKQFD
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHC
GLENDAGTRQLVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPK
CCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AFHHAQHSQSLNQSLSELNGDINVSFEFFPPSTPEMEQILWNSIRRLEPLNPKFVSVTY
CCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEE
GANSGVRDRTHGVIERIQQETDLVAAPHLTLVDASDEELLELAKHYWKSGIRDIVALRGD
CCCCCCCHHHHHHHHHHHHHHCEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
LPAGSPKPTRFAVDLVRLLRSVADFDISVAAYPEVHPDASNAQADLINLKRKIDAGASRA
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHH
ITQFFFDVESYLRFRDRCVAAGIDVEIVPGILPVTNFTQLKRFAGMTNVSLPGWLHKQFD
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHC
GLENDAGTRQLVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPK
CCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]