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The map label for this gene is hutI

Identifier: 16080988

GI number: 16080988

Start: 4045245

End: 4046510

Strand: Direct

Name: hutI

Synonym: BSU39370

Alternate gene names: 16080988

Gene position: 4045245-4046510 (Clockwise)

Preceding gene: 16080987

Following gene: 16080989

Centisome position: 95.96

GC content: 48.26

Gene sequence:

>1266_bases
ATGCCAAAGCAAATTGATACGATTCTCATCAACATCGGTCAGCTTTTGACGATGGAAAGCAGCGGTCCGCGGGCAGGCAA
AAGCATGCAGGATCTTCATGTGATCGAAGACGCTGTTGTCGGCATCCATGAACAGAAAATTGTGTTCGCCGGACAAAAAG
GGGCTGAAGCCGGTTACGAGGCAGACGAAATCATTGACTGTTCGGGACGCCTTGTAACGCCTGGCCTCGTTGATCCGCAC
ACCCATCTTGTATTTGGCGGATCTCGTGAAAAGGAGATGAACCTTAAGCTTCAGGGCATTTCATATTTGGATATTCTGGC
GCAGGGCGGCGGGATTCTGTCTACAGTCAAGGATACCAGGGCGGCTTCAGAAGAAGAGCTGCTGCAAAAAGCCCATTTTC
ATCTGCAAAGAATGCTGTCCTACGGCACAACCACAGCAGAAGTGAAAAGCGGCTACGGCTTGGAAAAAGAGACAGAGCTC
AAACAGTTGCGTGTCGCAAAGAAACTGCATGAAAGCCAGCCTGTTGACCTTGTTTCCACATTTATGGGAGCCCATGCGAT
TCCTCCCGAATATCAAAACGATCCAGATGATTTTCTGGATCAAATGCTTTCCCTTTTGCCGGAAATCAAAGAGCAGGAGC
TCGCCAGCTTTGCTGATATTTTTACAGAAACAGGCGTTTTTACAGTCAGCCAATCCCGCCGTTATTTACAAAAAGCAGCT
GAGGCAGGCTTCGGATTAAAAATACACGCAGATGAAATCGATCCGCTTGGAGGTGCTGAATTAGCAGGTAAGCTAAAGGC
CGTCTCAGCCGACCATTTGGTAGGTACATCGGACGAAGGCATAAAGAAATTGGCTGAGGCAGGAACGATCGCCGTCCTCT
TGCCGGGCACGACATTTTATCTTGGCAAAAGCACATACGCCAGGGCAAGAGCTATGATTGATGAAGGTGTATGTGTCAGC
TTGGCAACGGATTTTAATCCTGGCAGCTCGCCGACTGAAAATATCCAGCTGATTATGTCCATTGCCGCGCTTCATCTGAA
AATGACTGCAGAAGAAATATGGCACGCGGTCACAGTCAATGCCGCATATGCCATAGGAAAAGGAGAAGAAGCCGGTCAGC
TCAAAGCTGGCCGCTCAGCTGATCTTGTCATTTGGCAAGCCCCGAACTATATGTATATTCCGTATCACTACGGGGTCAAT
CATGTACATCAAGTCATGAAAAATGGAACAATTGTTGTCAACAGGGAGGGAGCCATTCTTGGATAA

Upstream 100 bases:

>100_bases
GGGCATTATCCGTCATGCGGATGCGGGTTATGAACGTGCCGTTGAAGTAGCGAAGGAACAGGACATTATCGTGCCGATGC
AAAAATAAAGGAGTGATGAC

Downstream 100 bases:

>100_bases
GTACCCTTTTCTCAGAGAGGCCGGATCTTCGTTTAAAGACCGGGATGTCACAAAAATGAGCGACCTGATCGCAACATGGG
ACGGCCAGGACATTAAGGGA

Product: imidazolonepropionase

Products: NA

Alternate protein names: Imidazolone-5-propionate hydrolase

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPKQIDTILINIGQLLTMESSGPRAGKSMQDLHVIEDAVVGIHEQKIVFAGQKGAEAGYEADEIIDCSGRLVTPGLVDPH
THLVFGGSREKEMNLKLQGISYLDILAQGGGILSTVKDTRAASEEELLQKAHFHLQRMLSYGTTTAEVKSGYGLEKETEL
KQLRVAKKLHESQPVDLVSTFMGAHAIPPEYQNDPDDFLDQMLSLLPEIKEQELASFADIFTETGVFTVSQSRRYLQKAA
EAGFGLKIHADEIDPLGGAELAGKLKAVSADHLVGTSDEGIKKLAEAGTIAVLLPGTTFYLGKSTYARARAMIDEGVCVS
LATDFNPGSSPTENIQLIMSIAALHLKMTAEEIWHAVTVNAAYAIGKGEEAGQLKAGRSADLVIWQAPNYMYIPYHYGVN
HVHQVMKNGTIVVNREGAILG

Sequences:

>Translated_421_residues
MPKQIDTILINIGQLLTMESSGPRAGKSMQDLHVIEDAVVGIHEQKIVFAGQKGAEAGYEADEIIDCSGRLVTPGLVDPH
THLVFGGSREKEMNLKLQGISYLDILAQGGGILSTVKDTRAASEEELLQKAHFHLQRMLSYGTTTAEVKSGYGLEKETEL
KQLRVAKKLHESQPVDLVSTFMGAHAIPPEYQNDPDDFLDQMLSLLPEIKEQELASFADIFTETGVFTVSQSRRYLQKAA
EAGFGLKIHADEIDPLGGAELAGKLKAVSADHLVGTSDEGIKKLAEAGTIAVLLPGTTFYLGKSTYARARAMIDEGVCVS
LATDFNPGSSPTENIQLIMSIAALHLKMTAEEIWHAVTVNAAYAIGKGEEAGQLKAGRSADLVIWQAPNYMYIPYHYGVN
HVHQVMKNGTIVVNREGAILG
>Mature_420_residues
PKQIDTILINIGQLLTMESSGPRAGKSMQDLHVIEDAVVGIHEQKIVFAGQKGAEAGYEADEIIDCSGRLVTPGLVDPHT
HLVFGGSREKEMNLKLQGISYLDILAQGGGILSTVKDTRAASEEELLQKAHFHLQRMLSYGTTTAEVKSGYGLEKETELK
QLRVAKKLHESQPVDLVSTFMGAHAIPPEYQNDPDDFLDQMLSLLPEIKEQELASFADIFTETGVFTVSQSRRYLQKAAE
AGFGLKIHADEIDPLGGAELAGKLKAVSADHLVGTSDEGIKKLAEAGTIAVLLPGTTFYLGKSTYARARAMIDEGVCVSL
ATDFNPGSSPTENIQLIMSIAALHLKMTAEEIWHAVTVNAAYAIGKGEEAGQLKAGRSADLVIWQAPNYMYIPYHYGVNH
VHQVMKNGTIVVNREGAILG

Specific function: Unknown

COG id: COG1228

COG function: function code Q; Imidazolonepropionase and related amidohydrolases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hutI family

Homologues:

Organism=Homo sapiens, GI223972677, Length=362, Percent_Identity=39.7790055248619, Blast_Score=245, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI17555004, Length=386, Percent_Identity=36.5284974093264, Blast_Score=237, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HUTI_BACSU (P42084)

Other databases:

- EMBL:   D31856
- EMBL:   AL009126
- PIR:   D69643
- RefSeq:   NP_391816.1
- PDB:   2BB0
- PDB:   2G3F
- PDBsum:   2BB0
- PDBsum:   2G3F
- ProteinModelPortal:   P42084
- SMR:   P42084
- EnsemblBacteria:   EBBACT00000002263
- GeneID:   937531
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39370
- NMPDR:   fig|224308.1.peg.3942
- GenoList:   BSU39370
- GeneTree:   EBGT00050000002251
- HOGENOM:   HBG686142
- OMA:   MNMACTL
- ProtClustDB:   PRK09356
- BioCyc:   BSUB:BSU39370-MONOMER
- BioCyc:   MetaCyc:HUTIBACSU-MONOMER
- BRENDA:   3.5.2.7
- GO:   GO:0005737
- HAMAP:   MF_00372
- InterPro:   IPR006680
- InterPro:   IPR005920
- InterPro:   IPR011059
- TIGRFAMs:   TIGR01224

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: =3.5.2.7

Molecular weight: Translated: 45565; Mature: 45433

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: BINDING 89-89 BINDING 102-102 BINDING 152-152 BINDING 185-185 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQIDTILINIGQLLTMESSGPRAGKSMQDLHVIEDAVVGIHEQKIVFAGQKGAEAGYE
CCCCHHHHHEEHHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
ADEIIDCSGRLVTPGLVDPHTHLVFGGSREKEMNLKLQGISYLDILAQGGGILSTVKDTR
HHHEECCCCCEECCCCCCCCCEEEECCCCCCCCEEEECCCHHHHHHHCCCCEEHHHHHHH
AASEEELLQKAHFHLQRMLSYGTTTAEVKSGYGLEKETELKQLRVAKKLHESQPVDLVST
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
FMGAHAIPPEYQNDPDDFLDQMLSLLPEIKEQELASFADIFTETGVFTVSQSRRYLQKAA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHH
EAGFGLKIHADEIDPLGGAELAGKLKAVSADHLVGTSDEGIKKLAEAGTIAVLLPGTTFY
HCCCCEEEECCCCCCCCCHHHHHHHHEECCCCCCCCCHHHHHHHHHCCCEEEEECCCEEE
LGKSTYARARAMIDEGVCVSLATDFNPGSSPTENIQLIMSIAALHLKMTAEEIWHAVTVN
ECCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
AAYAIGKGEEAGQLKAGRSADLVIWQAPNYMYIPYHYGVNHVHQVMKNGTIVVNREGAIL
EEEEECCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCHHHHHHHHHCCEEEEECCCCCC
G
C
>Mature Secondary Structure 
PKQIDTILINIGQLLTMESSGPRAGKSMQDLHVIEDAVVGIHEQKIVFAGQKGAEAGYE
CCCHHHHHEEHHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
ADEIIDCSGRLVTPGLVDPHTHLVFGGSREKEMNLKLQGISYLDILAQGGGILSTVKDTR
HHHEECCCCCEECCCCCCCCCEEEECCCCCCCCEEEECCCHHHHHHHCCCCEEHHHHHHH
AASEEELLQKAHFHLQRMLSYGTTTAEVKSGYGLEKETELKQLRVAKKLHESQPVDLVST
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
FMGAHAIPPEYQNDPDDFLDQMLSLLPEIKEQELASFADIFTETGVFTVSQSRRYLQKAA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHH
EAGFGLKIHADEIDPLGGAELAGKLKAVSADHLVGTSDEGIKKLAEAGTIAVLLPGTTFY
HCCCCEEEECCCCCCCCCHHHHHHHHEECCCCCCCCCHHHHHHHHHCCCEEEEECCCEEE
LGKSTYARARAMIDEGVCVSLATDFNPGSSPTENIQLIMSIAALHLKMTAEEIWHAVTVN
ECCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
AAYAIGKGEEAGQLKAGRSADLVIWQAPNYMYIPYHYGVNHVHQVMKNGTIVVNREGAIL
EEEEECCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCHHHHHHHHHCCEEEEECCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704263; 9384377