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The map label for this gene is hutU

Identifier: 16080987

GI number: 16080987

Start: 4043574

End: 4045232

Strand: Direct

Name: hutU

Synonym: BSU39360

Alternate gene names: 16080987

Gene position: 4043574-4045232 (Clockwise)

Preceding gene: 16080986

Following gene: 16080988

Centisome position: 95.92

GC content: 52.26

Gene sequence:

>1659_bases
ATGACTGATGTAAAAAAATCAATCCGTGCCAACAGAGGAACAGAGCTTGAATGCTTAGGATGGGAGCAAGAGGCAGTGCT
TCGGATGCTTCGAAACAACCTTGATCCAGAAGTCGCCGAGAAGCCGGAGGACCTCATCGTATATGGCGGGATTGGCAAAG
CCGCACGGGATTGGGACGCTTTTCACGCCATTGAGCATTCCTTGAAAACATTAAAAAATGATGAAACATTGCTCGTGCAA
TCCGGAAAGCCCGTTGGCATGTTCCGTACACACCCGCAGGCCCCTCGCGTGCTCCTAGCAAATTCTGTTCTTGTTCCGAA
GTGGGCAGACTGGGAGCATTTCCACGAACTTGAGAAAAAAGGGTTAATGATGTACGGACAGATGACGGCTGGCAGCTGGA
TTTACATTGGCTCACAAGGCATATTGCAAGGAACCTATGAAACCTTCGCTGAGCTGGCGCGACAGCACTTCGGGGGCAGC
TTAAAAGGAACGTTGACGCTGACAGCGGGTCTCGGCGGAATGGGAGGCGCCCAGCCGCTGTCAGTCACGATGAATGAAGG
CGTCGTGATTGCCGTCGAGGTTGACGAGAAACGCATCGACAAGCGGATCGAAACAAAATACTGCGATCGAAAAACAGCAT
CGATTGAGGAAGCCTTAGCTTGGGCTGAAGAGGCAAAGCTGGCAGGAAAACCTTTGTCCATTGCACTTCTCGGAAACGCA
GCTGAGGTGCATCACACACTTTTGAACCGAGGCGTCAAAATTGATATTGTGACAGATCAGACATCTGCCCATGACCCTTT
AATCGGTTATGTTCCCGAGGGCTATTCCCTTGATGAAGCTGACCGTTTGCGTCAAGACACTCCAGAGCTTTACGTGCGCC
TTGCGAAGCAAAGCATGAAAAAACATGTCGAAGCCATGCTGGCCTTTCAGCAAAAGGGCTCTATTGTATTTGACTACGGC
AACAATATCCGGCAAGTCGCAAAGGATGAAGGGCTGGAGAATGCCTTCGATTTTCCGGGGTTTGTCCCTGCCTACATCCG
CCCCTTATTCTGTGAAGGAAAAGGGCCGTTCCGCTGGGCGGCGCTTTCCGGAGATCCGGCAGATATTTACCGTACGGATG
CGTTATTAAAAGAGCTGTTCCCGACAAATAAAGCCCTTCACCGCTGGATTGATATGGCACAGGAAAAAGTCACCTTCCAA
GGCTTGCCGTCCCGTATCTGCTGGCTTGGATATGGAGAACGAAAAAAGATGGGCCTCGCCATTAACGAGCTGGTTAGAAC
CGGGGAGCTAAAAGCGCCTGTCGTTATTGGGAGAGATCATTTGGACTGCGGGTCTGTCGCCTCTCCAAACCGGGAAACAG
AAGCGATGAAGGATGGAAGTGACGCTGTCGGTGATTGGGCTGTGCTAAATGCGCTTGTCAACACGGCGGCGGGTGCAAGC
TGGGTTTCCTTCCACCATGGCGGCGGCGTCGGAATGGGCTATTCCCTTCATGCCGGCATGGTTGCAGTGGCCGATGGAAG
TGAGCTGGCTGACGAGCGGCTCGCGCGGGTCTTAACGAGCGACCCAGGAATGGGCATTATCCGTCATGCGGATGCGGGTT
ATGAACGTGCCGTTGAAGTAGCGAAGGAACAGGACATTATCGTGCCGATGCAAAAATAA

Upstream 100 bases:

>100_bases
ACCGTGTTTTTTCCTATGACATTGAACGCTTGACTGACTGGCTGAAGAAAGAATCTTTAATACCTGATCATCAGAATAAA
GAACTAAGGGGGATGAACAT

Downstream 100 bases:

>100_bases
AGGAGTGATGACATGCCAAAGCAAATTGATACGATTCTCATCAACATCGGTCAGCTTTTGACGATGGAAAGCAGCGGTCC
GCGGGCAGGCAAAAGCATGC

Product: urocanate hydratase

Products: NA

Alternate protein names: Urocanase; Imidazolonepropionate hydrolase

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MTDVKKSIRANRGTELECLGWEQEAVLRMLRNNLDPEVAEKPEDLIVYGGIGKAARDWDAFHAIEHSLKTLKNDETLLVQ
SGKPVGMFRTHPQAPRVLLANSVLVPKWADWEHFHELEKKGLMMYGQMTAGSWIYIGSQGILQGTYETFAELARQHFGGS
LKGTLTLTAGLGGMGGAQPLSVTMNEGVVIAVEVDEKRIDKRIETKYCDRKTASIEEALAWAEEAKLAGKPLSIALLGNA
AEVHHTLLNRGVKIDIVTDQTSAHDPLIGYVPEGYSLDEADRLRQDTPELYVRLAKQSMKKHVEAMLAFQQKGSIVFDYG
NNIRQVAKDEGLENAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPADIYRTDALLKELFPTNKALHRWIDMAQEKVTFQ
GLPSRICWLGYGERKKMGLAINELVRTGELKAPVVIGRDHLDCGSVASPNRETEAMKDGSDAVGDWAVLNALVNTAAGAS
WVSFHHGGGVGMGYSLHAGMVAVADGSELADERLARVLTSDPGMGIIRHADAGYERAVEVAKEQDIIVPMQK

Sequences:

>Translated_552_residues
MTDVKKSIRANRGTELECLGWEQEAVLRMLRNNLDPEVAEKPEDLIVYGGIGKAARDWDAFHAIEHSLKTLKNDETLLVQ
SGKPVGMFRTHPQAPRVLLANSVLVPKWADWEHFHELEKKGLMMYGQMTAGSWIYIGSQGILQGTYETFAELARQHFGGS
LKGTLTLTAGLGGMGGAQPLSVTMNEGVVIAVEVDEKRIDKRIETKYCDRKTASIEEALAWAEEAKLAGKPLSIALLGNA
AEVHHTLLNRGVKIDIVTDQTSAHDPLIGYVPEGYSLDEADRLRQDTPELYVRLAKQSMKKHVEAMLAFQQKGSIVFDYG
NNIRQVAKDEGLENAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPADIYRTDALLKELFPTNKALHRWIDMAQEKVTFQ
GLPSRICWLGYGERKKMGLAINELVRTGELKAPVVIGRDHLDCGSVASPNRETEAMKDGSDAVGDWAVLNALVNTAAGAS
WVSFHHGGGVGMGYSLHAGMVAVADGSELADERLARVLTSDPGMGIIRHADAGYERAVEVAKEQDIIVPMQK
>Mature_551_residues
TDVKKSIRANRGTELECLGWEQEAVLRMLRNNLDPEVAEKPEDLIVYGGIGKAARDWDAFHAIEHSLKTLKNDETLLVQS
GKPVGMFRTHPQAPRVLLANSVLVPKWADWEHFHELEKKGLMMYGQMTAGSWIYIGSQGILQGTYETFAELARQHFGGSL
KGTLTLTAGLGGMGGAQPLSVTMNEGVVIAVEVDEKRIDKRIETKYCDRKTASIEEALAWAEEAKLAGKPLSIALLGNAA
EVHHTLLNRGVKIDIVTDQTSAHDPLIGYVPEGYSLDEADRLRQDTPELYVRLAKQSMKKHVEAMLAFQQKGSIVFDYGN
NIRQVAKDEGLENAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPADIYRTDALLKELFPTNKALHRWIDMAQEKVTFQG
LPSRICWLGYGERKKMGLAINELVRTGELKAPVVIGRDHLDCGSVASPNRETEAMKDGSDAVGDWAVLNALVNTAAGASW
VSFHHGGGVGMGYSLHAGMVAVADGSELADERLARVLTSDPGMGIIRHADAGYERAVEVAKEQDIIVPMQK

Specific function: Unknown

COG id: COG2987

COG function: function code E; Urocanate hydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the urocanase family

Homologues:

Organism=Homo sapiens, GI21389467, Length=561, Percent_Identity=32.620320855615, Blast_Score=300, Evalue=3e-81,
Organism=Homo sapiens, GI260306182, Length=621, Percent_Identity=30.1127214170692, Blast_Score=278, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI71997891, Length=545, Percent_Identity=32.1100917431193, Blast_Score=302, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HUTU_BACSU (P25503)

Other databases:

- EMBL:   D31856
- EMBL:   AL009126
- EMBL:   M20659
- PIR:   G69643
- RefSeq:   NP_391815.1
- PDB:   2FKN
- PDBsum:   2FKN
- ProteinModelPortal:   P25503
- SMR:   P25503
- EnsemblBacteria:   EBBACT00000000384
- GeneID:   937542
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39360
- NMPDR:   fig|224308.1.peg.3941
- GenoList:   BSU39360
- GeneTree:   EBGT00050000001977
- HOGENOM:   HBG305285
- OMA:   IRQMAFE
- ProtClustDB:   PRK05414
- BioCyc:   BSUB:BSU39360-MONOMER
- BioCyc:   MetaCyc:HUTUBACSU-MONOMER
- BRENDA:   4.2.1.49
- GO:   GO:0005737
- HAMAP:   MF_00577
- InterPro:   IPR000193
- PIRSF:   PIRSF001423
- TIGRFAMs:   TIGR01228

Pfam domain/function: PF01175 Urocanase; SSF111326 Urocanase

EC number: =4.2.1.49

Molecular weight: Translated: 60601; Mature: 60470

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01233 UROCANASE

Important sites: ACT_SITE 407-407

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVKKSIRANRGTELECLGWEQEAVLRMLRNNLDPEVAEKPEDLIVYGGIGKAARDWDA
CCCHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCHHHCCCCCEEEECCCCCCHHHHHH
FHAIEHSLKTLKNDETLLVQSGKPVGMFRTHPQAPRVLLANSVLVPKWADWEHFHELEKK
HHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCCEEEECCEECCCCCCHHHHHHHHHC
GLMMYGQMTAGSWIYIGSQGILQGTYETFAELARQHFGGSLKGTLTLTAGLGGMGGAQPL
CEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCE
SVTMNEGVVIAVEVDEKRIDKRIETKYCDRKTASIEEALAWAEEAKLAGKPLSIALLGNA
EEEECCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCH
AEVHHTLLNRGVKIDIVTDQTSAHDPLIGYVPEGYSLDEADRLRQDTPELYVRLAKQSMK
HHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
KHVEAMLAFQQKGSIVFDYGNNIRQVAKDEGLENAFDFPGFVPAYIRPLFCEGKGPFRWA
HHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHCEEEECCCCCEEEE
ALSGDPADIYRTDALLKELFPTNKALHRWIDMAQEKVTFQGLPSRICWLGYGERKKMGLA
EECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHH
INELVRTGELKAPVVIGRDHLDCGSVASPNRETEAMKDGSDAVGDWAVLNALVNTAAGAS
HHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
WVSFHHGGGVGMGYSLHAGMVAVADGSELADERLARVLTSDPGMGIIRHADAGYERAVEV
EEEEECCCCCCCCEEECCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
AKEQDIIVPMQK
HCCCCEEECCCC
>Mature Secondary Structure 
TDVKKSIRANRGTELECLGWEQEAVLRMLRNNLDPEVAEKPEDLIVYGGIGKAARDWDA
CCHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCHHHCCCCCEEEECCCCCCHHHHHH
FHAIEHSLKTLKNDETLLVQSGKPVGMFRTHPQAPRVLLANSVLVPKWADWEHFHELEKK
HHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCCEEEECCEECCCCCCHHHHHHHHHC
GLMMYGQMTAGSWIYIGSQGILQGTYETFAELARQHFGGSLKGTLTLTAGLGGMGGAQPL
CEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCE
SVTMNEGVVIAVEVDEKRIDKRIETKYCDRKTASIEEALAWAEEAKLAGKPLSIALLGNA
EEEECCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCH
AEVHHTLLNRGVKIDIVTDQTSAHDPLIGYVPEGYSLDEADRLRQDTPELYVRLAKQSMK
HHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
KHVEAMLAFQQKGSIVFDYGNNIRQVAKDEGLENAFDFPGFVPAYIRPLFCEGKGPFRWA
HHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCHHHHCEEEECCCCCEEEE
ALSGDPADIYRTDALLKELFPTNKALHRWIDMAQEKVTFQGLPSRICWLGYGERKKMGLA
EECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHH
INELVRTGELKAPVVIGRDHLDCGSVASPNRETEAMKDGSDAVGDWAVLNALVNTAAGAS
HHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
WVSFHHGGGVGMGYSLHAGMVAVADGSELADERLARVLTSDPGMGIIRHADAGYERAVEV
EEEEECCCCCCCCEEECCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
AKEQDIIVPMQK
HCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704263; 9384377; 2454913