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The map label for this gene is sacX

Identifier: 16080892

GI number: 16080892

Start: 3942234

End: 3943613

Strand: Direct

Name: sacX

Synonym: BSU38410

Alternate gene names: 16080892

Gene position: 3942234-3943613 (Clockwise)

Preceding gene: 16080891

Following gene: 16080893

Centisome position: 93.52

GC content: 48.41

Gene sequence:

>1380_bases
TTGCATAAAGAGATTGCAAAAGAATTACTGCTGCTCGCAGGAGGAAAAAACAATATTATCAGCATCAGCCATTGTACGAC
CCGTCTTCGTTTTGATGTAAAGGACGAGACGAAAATTGATATACATGCCATTGAGAACCTGCAAGGTGTGCAGGGCACCT
TTTTCCGCTATGGGCTTTTCCAGATTATCTTCGGAGCAGGCGTCGTCAACAAAATATATAAAGAAGTCGTTCATGTATGG
GAGACCGCGCCTTCTGAAGAGCCTGTCCACCAGAAAAAAGCCAGCCGGAAGCTGAACCCTGCCGCAGCTTTTGCGAAAAC
ACTGTCCGATATTTTTGTGCCGATTATTCCGGCAATAACAGCAAGCGGCCTGCTCATGGGGCTGATCGGCATGATTAAGG
TGTTTCATTGGTTCGCTGCCGGCAGCCCATGGATTAAAATGCTCGACCTCGTGTCCAGCACAGCCTTTATTTTACTGCCG
ATTTTAGTCGGCTTCAGTGCTGCACGGCAATTTGGCAGCAACCCATACCTAGGGGCAGTGATTGCAGGGCTTTTGACGCA
TCCTGATCTGCTGGACCCGTCTATGCTCGGCAGCAAAACCCCGTCTTCTTTAGACATATGGGGGCTTCATATCCCAATGA
TGGGCTATCAGGGTTCCATGATACCGATTCTTCTTTCCGTTTTTGTCATGAGCAAAATTGAGAAGCTATTAAAATCGATT
GTGCCGAAGTCGCTTGATGTCGTGATCATTCCCTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAA
TCCCGCCGCGTCCATCATCGGCCAGATCATGACACAATCGATCGTCTATATTTATGATCACGCCGGCATTGCTGCCGGAG
CTCTTTTCGGCGGCATCTACTCCACCATCGTCCTATCTGGATTGCATCATAGCTTTTATGCAATTGAGGCAACGCTGCTT
GCTAATCCGCATGTCGGTGTCAACTTTTTAGTGCCGATATGGTCGATGGCGAATGTGGCCCAAGGCGGAGCGGGGCTTGC
GGTATTCCTCAAAACGAAACAATCAAGCCTGAAGAAAATTGCGCTCCCCGCTTCTCTGACCGCGTTTTTAGGCATTGTTG
AGCCGATTGTATTCGGGGTAAATCTCAAATTGATCCGTCCCTTTATCGGAGCAGCCATCGGCGGTGCTATTGGCGGAGCA
TACGTGGTTGCGGTACAAGTTGTGGCAAATTCCTACGGACTGACAGGCATTCCGATGATTTCAATCGTGCTGCCGTTTGG
CGCCGCTAATTTTGTTCATTATATGATCGGTTTCTTGATCGCAGCCGTCTCTGCTTTTATAGCTACATTGTTTCTCGGGT
TTAAAGAAGAGACAGAATAA

Upstream 100 bases:

>100_bases
TTTCAGCCGGTATTTCTCACTTTTTTCATTTTGATTGTCAACTTTTCATTTGATACGATGAACAAGCCGACTTTTCTTCA
GCAAAGGAGGTTTTAATTGC

Downstream 100 bases:

>100_bases
CTGGATTTATTCGATTTCATTCATAAAACGGGGGATGAAAGGACAAAAAAGCTATGAAAATTAAAAGAATCTTAAATCAT
AATGCTATCGTCGTAAAGGA

Product: negative regulator of SacY

Products: NA

Alternate protein names: PTS system sac EIIBC component; Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MHKEIAKELLLLAGGKNNIISISHCTTRLRFDVKDETKIDIHAIENLQGVQGTFFRYGLFQIIFGAGVVNKIYKEVVHVW
ETAPSEEPVHQKKASRKLNPAAAFAKTLSDIFVPIIPAITASGLLMGLIGMIKVFHWFAAGSPWIKMLDLVSSTAFILLP
ILVGFSAARQFGSNPYLGAVIAGLLTHPDLLDPSMLGSKTPSSLDIWGLHIPMMGYQGSMIPILLSVFVMSKIEKLLKSI
VPKSLDVVIIPFITVMVTGCLALIVMNPAASIIGQIMTQSIVYIYDHAGIAAGALFGGIYSTIVLSGLHHSFYAIEATLL
ANPHVGVNFLVPIWSMANVAQGGAGLAVFLKTKQSSLKKIALPASLTAFLGIVEPIVFGVNLKLIRPFIGAAIGGAIGGA
YVVAVQVVANSYGLTGIPMISIVLPFGAANFVHYMIGFLIAAVSAFIATLFLGFKEETE

Sequences:

>Translated_459_residues
MHKEIAKELLLLAGGKNNIISISHCTTRLRFDVKDETKIDIHAIENLQGVQGTFFRYGLFQIIFGAGVVNKIYKEVVHVW
ETAPSEEPVHQKKASRKLNPAAAFAKTLSDIFVPIIPAITASGLLMGLIGMIKVFHWFAAGSPWIKMLDLVSSTAFILLP
ILVGFSAARQFGSNPYLGAVIAGLLTHPDLLDPSMLGSKTPSSLDIWGLHIPMMGYQGSMIPILLSVFVMSKIEKLLKSI
VPKSLDVVIIPFITVMVTGCLALIVMNPAASIIGQIMTQSIVYIYDHAGIAAGALFGGIYSTIVLSGLHHSFYAIEATLL
ANPHVGVNFLVPIWSMANVAQGGAGLAVFLKTKQSSLKKIALPASLTAFLGIVEPIVFGVNLKLIRPFIGAAIGGAIGGA
YVVAVQVVANSYGLTGIPMISIVLPFGAANFVHYMIGFLIAAVSAFIATLFLGFKEETE
>Mature_459_residues
MHKEIAKELLLLAGGKNNIISISHCTTRLRFDVKDETKIDIHAIENLQGVQGTFFRYGLFQIIFGAGVVNKIYKEVVHVW
ETAPSEEPVHQKKASRKLNPAAAFAKTLSDIFVPIIPAITASGLLMGLIGMIKVFHWFAAGSPWIKMLDLVSSTAFILLP
ILVGFSAARQFGSNPYLGAVIAGLLTHPDLLDPSMLGSKTPSSLDIWGLHIPMMGYQGSMIPILLSVFVMSKIEKLLKSI
VPKSLDVVIIPFITVMVTGCLALIVMNPAASIIGQIMTQSIVYIYDHAGIAAGALFGGIYSTIVLSGLHHSFYAIEATLL
ANPHVGVNFLVPIWSMANVAQGGAGLAVFLKTKQSSLKKIALPASLTAFLGIVEPIVFGVNLKLIRPFIGAAIGGAIGGA
YVVAVQVVANSYGLTGIPMISIVLPFGAANFVHYMIGFLIAAVSAFIATLFLGFKEETE

Specific function: Negatively regulates sacY

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain

Homologues:

Organism=Escherichia coli, GI1790159, Length=456, Percent_Identity=30.9210526315789, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI2367362, Length=429, Percent_Identity=28.6713286713287, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI48994906, Length=465, Percent_Identity=29.247311827957, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788769, Length=486, Percent_Identity=28.8065843621399, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SACX_BACSU (P15400)

Other databases:

- EMBL:   M29333
- EMBL:   X73124
- EMBL:   AL009126
- EMBL:   X52480
- PIR:   JU0293
- RefSeq:   NP_391720.1
- ProteinModelPortal:   P15400
- SMR:   P15400
- EnsemblBacteria:   EBBACT00000003259
- GeneID:   937333
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38410
- NMPDR:   fig|224308.1.peg.3846
- GenoList:   BSU38410
- GeneTree:   EBGT00050000000033
- HOGENOM:   HBG673521
- PhylomeDB:   P15400
- ProtClustDB:   CLSK491528
- BioCyc:   BSUB:BSU38410-MONOMER
- BRENDA:   2.7.1.69
- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973
- Gene3D:   G3DSA:3.30.1360.60
- TIGRFAMs:   TIGR00826
- TIGRFAMs:   TIGR01996

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC; SSF55604 PTS_EIIB

EC number: =2.7.1.69

Molecular weight: Translated: 49025; Mature: 49025

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS51098 PTS_EIIB_TYPE_1; PS01035 PTS_EIIB_TYPE_1_CYS; PS51103 PTS_EIIC_TYPE_1

Important sites: ACT_SITE 25-25

Signals:

None

Transmembrane regions:

HASH(0x13087bac)-; HASH(0x1224330c)-; HASH(0x12f970c0)-; HASH(0x133d0b28)-; HASH(0x133e0ce4)-; HASH(0x133cb558)-; HASH(0x12c0f2c4)-; HASH(0x12c75d9c)-; HASH(0x133d2fb0)-; HASH(0x133cb4c8)-; HASH(0x133c5248)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKEIAKELLLLAGGKNNIISISHCTTRLRFDVKDETKIDIHAIENLQGVQGTFFRYGLF
CCHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCCEEEHHHHHCCCCCCHHHHHHHHH
QIIFGAGVVNKIYKEVVHVWETAPSEEPVHQKKASRKLNPAAAFAKTLSDIFVPIIPAIT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ASGLLMGLIGMIKVFHWFAAGSPWIKMLDLVSSTAFILLPILVGFSAARQFGSNPYLGAV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
IAGLLTHPDLLDPSMLGSKTPSSLDIWGLHIPMMGYQGSMIPILLSVFVMSKIEKLLKSI
HHHHHCCCCCCCCHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
VPKSLDVVIIPFITVMVTGCLALIVMNPAASIIGQIMTQSIVYIYDHAGIAAGALFGGIY
CCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHH
STIVLSGLHHSFYAIEATLLANPHVGVNFLVPIWSMANVAQGGAGLAVFLKTKQSSLKKI
HHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHH
ALPASLTAFLGIVEPIVFGVNLKLIRPFIGAAIGGAIGGAYVVAVQVVANSYGLTGIPMI
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
SIVLPFGAANFVHYMIGFLIAAVSAFIATLFLGFKEETE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MHKEIAKELLLLAGGKNNIISISHCTTRLRFDVKDETKIDIHAIENLQGVQGTFFRYGLF
CCHHHHHHHHHHCCCCCCEEEEEECCEEEEEECCCCCEEEHHHHHCCCCCCHHHHHHHHH
QIIFGAGVVNKIYKEVVHVWETAPSEEPVHQKKASRKLNPAAAFAKTLSDIFVPIIPAIT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ASGLLMGLIGMIKVFHWFAAGSPWIKMLDLVSSTAFILLPILVGFSAARQFGSNPYLGAV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
IAGLLTHPDLLDPSMLGSKTPSSLDIWGLHIPMMGYQGSMIPILLSVFVMSKIEKLLKSI
HHHHHCCCCCCCCHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
VPKSLDVVIIPFITVMVTGCLALIVMNPAASIIGQIMTQSIVYIYDHAGIAAGALFGGIY
CCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHH
STIVLSGLHHSFYAIEATLLANPHVGVNFLVPIWSMANVAQGGAGLAVFLKTKQSSLKKI
HHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHH
ALPASLTAFLGIVEPIVFGVNLKLIRPFIGAAIGGAIGGAYVVAVQVVANSYGLTGIPMI
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
SIVLPFGAANFVHYMIGFLIAAVSAFIATLFLGFKEETE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2116367; 7934828; 9384377; 1806041