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The map label for this gene is sacY

Identifier: 16080893

GI number: 16080893

Start: 3943667

End: 3944509

Strand: Direct

Name: sacY

Synonym: BSU38420

Alternate gene names: 16080893

Gene position: 3943667-3944509 (Clockwise)

Preceding gene: 16080892

Following gene: 16080896

Centisome position: 93.55

GC content: 39.98

Gene sequence:

>843_bases
ATGAAAATTAAAAGAATCTTAAATCATAATGCTATCGTCGTAAAGGATCAAAATGAAGAGAAGATTCTCTTGGGTGCAGG
AATTGCGTTTAACAAAAAGAAGAATGATATTGTCGATCCGTCAAAAATAGAAAAAACCTTTATCAGAAAAGATACACCTG
ACTATAAGCAGTTCGAAGAGATTTTAGAAACATTGCCTGAAGACCACATTCAGATTTCTGAGCAAATTATCTCTCATGCC
GAAAAAGAGCTGAACATCAAAATCAACGAGCGCATTCATGTCGCTTTTTCAGACCATCTTTCTTTTGCAATTGAACGCCT
GAGCAATGGGATGGTTATCAAAAATCCGCTGCTGAATGAAATCAAAGTCCTTTATCCAAAGGAGTTCCAGATCGGCTTAT
GGGCCAGAGCACTGATTAAAGATAAACTGGGGATTCACATTCCTGATGATGAAATCGGCAATATCGCCATGCATATCCAC
ACAGCAAGAAACAATGCCGGCGATATGACACAAACGCTTGATATTACAACAATGATCCGTGATATTATCGAGATTATCGA
AATTCAACTGTCTATTAATATCGTTGAAGATACCATCTCTTATGAAAGGCTCGTGACCCATCTCCGCTTTGCCATTCAGC
ATATCAAAGCAGGCGAATCCATTTACGAGCTGGACGCAGAAATGATTGACATCATTAAAGAGAAGTTTAAGGATGCCTTC
CTGTGTGCCCTAAGCATCGGCACCTTTGTGAAGAAGGAATACGGCTTTGAGTTTCCTGAAAAAGAATTGTGCTACATCGC
CATGCATATTCAGCGGTTCTACCAACGGTCAGTCGCACGCTGA

Upstream 100 bases:

>100_bases
CTTTTATAGCTACATTGTTTCTCGGGTTTAAAGAAGAGACAGAATAACTGGATTTATTCGATTTCATTCATAAAACGGGG
GATGAAAGGACAAAAAAGCT

Downstream 100 bases:

>100_bases
GACAAACAAAAAACGCTTTTGATCATCTCAAAAGCGTTTTTTTATCTGATTTATTGGTTGATCGCCGGATTCCCAATCGT
ATTCCACTTCGTATAGGACA

Product: transcriptional antiterminator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKIKRILNHNAIVVKDQNEEKILLGAGIAFNKKKNDIVDPSKIEKTFIRKDTPDYKQFEEILETLPEDHIQISEQIISHA
EKELNIKINERIHVAFSDHLSFAIERLSNGMVIKNPLLNEIKVLYPKEFQIGLWARALIKDKLGIHIPDDEIGNIAMHIH
TARNNAGDMTQTLDITTMIRDIIEIIEIQLSINIVEDTISYERLVTHLRFAIQHIKAGESIYELDAEMIDIIKEKFKDAF
LCALSIGTFVKKEYGFEFPEKELCYIAMHIQRFYQRSVAR

Sequences:

>Translated_280_residues
MKIKRILNHNAIVVKDQNEEKILLGAGIAFNKKKNDIVDPSKIEKTFIRKDTPDYKQFEEILETLPEDHIQISEQIISHA
EKELNIKINERIHVAFSDHLSFAIERLSNGMVIKNPLLNEIKVLYPKEFQIGLWARALIKDKLGIHIPDDEIGNIAMHIH
TARNNAGDMTQTLDITTMIRDIIEIIEIQLSINIVEDTISYERLVTHLRFAIQHIKAGESIYELDAEMIDIIKEKFKDAF
LCALSIGTFVKKEYGFEFPEKELCYIAMHIQRFYQRSVAR
>Mature_280_residues
MKIKRILNHNAIVVKDQNEEKILLGAGIAFNKKKNDIVDPSKIEKTFIRKDTPDYKQFEEILETLPEDHIQISEQIISHA
EKELNIKINERIHVAFSDHLSFAIERLSNGMVIKNPLLNEIKVLYPKEFQIGLWARALIKDKLGIHIPDDEIGNIAMHIH
TARNNAGDMTQTLDITTMIRDIIEIIEIQLSINIVEDTISYERLVTHLRFAIQHIKAGESIYELDAEMIDIIKEKFKDAF
LCALSIGTFVKKEYGFEFPEKELCYIAMHIQRFYQRSVAR

Specific function: In the presence of sucrose, SacY is activated and prevents premature termination of transcription by binding to a RNA-antiterminator (RAT) sequence (partially overlapping with the terminator sequence) located upstream of the sacB gene. Formation of the Sa

COG id: COG3711

COG function: function code K; Transcriptional antiterminator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PRD domains

Homologues:

Organism=Escherichia coli, GI1790160, Length=273, Percent_Identity=35.1648351648352, Blast_Score=187, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SACY_BACSU (P15401)

Other databases:

- EMBL:   M29333
- EMBL:   X52480
- EMBL:   X73124
- EMBL:   AL009126
- PIR:   JU0294
- RefSeq:   NP_391721.1
- PDB:   1AUU
- PDBsum:   1AUU
- ProteinModelPortal:   P15401
- SMR:   P15401
- EnsemblBacteria:   EBBACT00000000830
- GeneID:   937335
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38420
- NMPDR:   fig|224308.1.peg.3847
- GenoList:   BSU38420
- GeneTree:   EBGT00050000000027
- HOGENOM:   HBG515578
- OMA:   ERIHVAF
- PhylomeDB:   P15401
- ProtClustDB:   CLSK888093
- BioCyc:   BSUB:BSU38420-MONOMER
- GO:   GO:0006350
- InterPro:   IPR004341
- InterPro:   IPR011608
- InterPro:   IPR001550
- Gene3D:   G3DSA:2.30.24.10
- Gene3D:   G3DSA:1.10.1790.10

Pfam domain/function: PF03123 CAT_RBD; PF00874 PRD; SSF50151 CAT_RBD; SSF63520 PRD

EC number: NA

Molecular weight: Translated: 32466; Mature: 32466

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00654 PRD_1; PS51372 PRD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRILNHNAIVVKDQNEEKILLGAGIAFNKKKNDIVDPSKIEKTFIRKDTPDYKQFEE
CCCHHHHCCCEEEEECCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
ILETLPEDHIQISEQIISHAEKELNIKINERIHVAFSDHLSFAIERLSNGMVIKNPLLNE
HHHHCCHHHHHHHHHHHHHHHHHCEEEECCEEEEEEHHHHHHHHHHHCCCEEEECCCHHH
IKVLYPKEFQIGLWARALIKDKLGIHIPDDEIGNIAMHIHTARNNAGDMTQTLDITTMIR
HEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
DIIEIIEIQLSINIVEDTISYERLVTHLRFAIQHIKAGESIYELDAEMIDIIKEKFKDAF
HHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LCALSIGTFVKKEYGFEFPEKELCYIAMHIQRFYQRSVAR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIKRILNHNAIVVKDQNEEKILLGAGIAFNKKKNDIVDPSKIEKTFIRKDTPDYKQFEE
CCCHHHHCCCEEEEECCCCCEEEEECCEEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
ILETLPEDHIQISEQIISHAEKELNIKINERIHVAFSDHLSFAIERLSNGMVIKNPLLNE
HHHHCCHHHHHHHHHHHHHHHHHCEEEECCEEEEEEHHHHHHHHHHHCCCEEEECCCHHH
IKVLYPKEFQIGLWARALIKDKLGIHIPDDEIGNIAMHIHTARNNAGDMTQTLDITTMIR
HEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
DIIEIIEIQLSINIVEDTISYERLVTHLRFAIQHIKAGESIYELDAEMIDIIKEKFKDAF
HHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LCALSIGTFVKKEYGFEFPEKELCYIAMHIQRFYQRSVAR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2116367; 1806041; 7934828; 9384377; 8535520; 9202047; 9261875; 9305643