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The map label for this gene is nfrA

Identifier: 16080862

GI number: 16080862

Start: 3911477

End: 3912226

Strand: Reverse

Name: nfrA

Synonym: BSU38110

Alternate gene names: 16080862

Gene position: 3912226-3911477 (Counterclockwise)

Preceding gene: 16080863

Following gene: 16080861

Centisome position: 92.8

GC content: 43.73

Gene sequence:

>750_bases
ATGAATAACACAATCGAAACCATTTTGAATCATCGGTCAATCCGGTCTTTTACTGATCAGCTTTTGACAGCTGAAGAAAT
TGATACATTAGTGAAAAGTGCGCAGGCTGCGTCTACATCCAGCTATGTGCAGGCATATTCGATTATCGGGGTTTCTGATC
CTGAGAAAAAACGTGAGCTGTCCGTGCTTGCTGGGAATCAGCCTTACGTTGAGAAGAACGGACACTTTTTTGTATTTTGT
GCGGATTTGTACCGCCATCAACAACTGGCTGAGGAAAAAGGTGAACATATTTCTGAGCTGCTCGAGAATACAGAAATGTT
TATGGTTAGCTTGATTGATGCGGCGCTGGCAGCGCAAAATATGAGCATTGCCGCTGAATCCATGGGGCTTGGCATTTGCT
ATATCGGCGGCATTCGGAATGAATTGGACAAGGTGACAGAGGTGCTGCAAACGCCTGACCATGTGCTCCCGCTTTTCGGA
CTGGCAGTAGGCCATCCTGCGAACCTTTCCGGCAAAAAACCGAGATTGCCAAAGCAGGCCGTTTATCATGAAAATACGTA
TAATGTGAACACAGACGATTTCCGTCATACTATGAATACGTATGACAAAACCATTTCTGATTACTATAGAGAAAGAACAA
ACGGGAAGCGGGAAGAAACGTGGTCCGATCAAATACTGAACTTTATGAAGCAAAAGCCGCGTACGTATTTAAATGACTAC
GTGAAAGAAAAGGGCTTTAATAAAAACTAA

Upstream 100 bases:

>100_bases
AGACAGCCTTTACAGAGGCTGTCTTTTTTTGTGCAGAGCATTTCACTTTTGAGATCACTTTTTTTCGGATATGATAAAAA
GTGATAAGGAGGTTTTTGTG

Downstream 100 bases:

>100_bases
AGCAAACCTCTCCGTCTGGAGGGGTTTTTATGTTTCACGTGTAACATATTCCGACAAATAGGGACATGCTTTCACAAATA
AATGGCGAATGATAGGGGAC

Product: FMN-containing NADPH-linked nitro/flavin reductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNNTIETILNHRSIRSFTDQLLTAEEIDTLVKSAQAASTSSYVQAYSIIGVSDPEKKRELSVLAGNQPYVEKNGHFFVFC
ADLYRHQQLAEEKGEHISELLENTEMFMVSLIDAALAAQNMSIAAESMGLGICYIGGIRNELDKVTEVLQTPDHVLPLFG
LAVGHPANLSGKKPRLPKQAVYHENTYNVNTDDFRHTMNTYDKTISDYYRERTNGKREETWSDQILNFMKQKPRTYLNDY
VKEKGFNKN

Sequences:

>Translated_249_residues
MNNTIETILNHRSIRSFTDQLLTAEEIDTLVKSAQAASTSSYVQAYSIIGVSDPEKKRELSVLAGNQPYVEKNGHFFVFC
ADLYRHQQLAEEKGEHISELLENTEMFMVSLIDAALAAQNMSIAAESMGLGICYIGGIRNELDKVTEVLQTPDHVLPLFG
LAVGHPANLSGKKPRLPKQAVYHENTYNVNTDDFRHTMNTYDKTISDYYRERTNGKREETWSDQILNFMKQKPRTYLNDY
VKEKGFNKN
>Mature_249_residues
MNNTIETILNHRSIRSFTDQLLTAEEIDTLVKSAQAASTSSYVQAYSIIGVSDPEKKRELSVLAGNQPYVEKNGHFFVFC
ADLYRHQQLAEEKGEHISELLENTEMFMVSLIDAALAAQNMSIAAESMGLGICYIGGIRNELDKVTEVLQTPDHVLPLFG
LAVGHPANLSGKKPRLPKQAVYHENTYNVNTDDFRHTMNTYDKTISDYYRERTNGKREETWSDQILNFMKQKPRTYLNDY
VKEKGFNKN

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family

Homologues:

Organism=Escherichia coli, GI1787075, Length=246, Percent_Identity=40.2439024390244, Blast_Score=187, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFRA1_BACSU (P39605)

Other databases:

- EMBL:   X73124
- EMBL:   AL009126
- PIR:   S39698
- RefSeq:   NP_391690.1
- PDB:   3N2S
- PDBsum:   3N2S
- ProteinModelPortal:   P39605
- SMR:   P39605
- EnsemblBacteria:   EBBACT00000003819
- GeneID:   937279
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU38110
- NMPDR:   fig|224308.1.peg.3816
- GenoList:   BSU38110
- GeneTree:   EBGT00050000001304
- HOGENOM:   HBG532063
- OMA:   TILTEYD
- PhylomeDB:   P39605
- ProtClustDB:   CLSK888095
- BioCyc:   BSUB:BSU38110-MONOMER
- InterPro:   IPR016446
- InterPro:   IPR000415
- PIRSF:   PIRSF005426

Pfam domain/function: PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: =1.5.1.29

Molecular weight: Translated: 28320; Mature: 28320

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTIETILNHRSIRSFTDQLLTAEEIDTLVKSAQAASTSSYVQAYSIIGVSDPEKKREL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHH
SVLAGNQPYVEKNGHFFVFCADLYRHQQLAEEKGEHISELLENTEMFMVSLIDAALAAQN
HHEECCCCCEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSIAAESMGLGICYIGGIRNELDKVTEVLQTPDHVLPLFGLAVGHPANLSGKKPRLPKQA
HHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VYHENTYNVNTDDFRHTMNTYDKTISDYYRERTNGKREETWSDQILNFMKQKPRTYLNDY
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHH
VKEKGFNKN
HHHCCCCCC
>Mature Secondary Structure
MNNTIETILNHRSIRSFTDQLLTAEEIDTLVKSAQAASTSSYVQAYSIIGVSDPEKKREL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHH
SVLAGNQPYVEKNGHFFVFCADLYRHQQLAEEKGEHISELLENTEMFMVSLIDAALAAQN
HHEECCCCCEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSIAAESMGLGICYIGGIRNELDKVTEVLQTPDHVLPLFGLAVGHPANLSGKKPRLPKQA
HHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VYHENTYNVNTDDFRHTMNTYDKTISDYYRERTNGKREETWSDQILNFMKQKPRTYLNDY
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHH
VKEKGFNKN
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433